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Protein

DNA replication licensing factor MCM3 homolog 2

Gene

ROA2

Organism
Zea mays (Maize)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Acts as a factor that allows the DNA to undergo a single round of replication per cell cycle. Required for DNA replication and cell proliferation (By similarity). May act as a component of the MCM complex which is the putative replicative helicase of the replication licensing system in eukaryotic cells.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi340 – 3478ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Cell cycle, DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor MCM3 homolog 2 (EC:3.6.4.12)
Alternative name(s):
Replication origin activator 2
Short name:
ROA-2
Gene namesi
Name:ROA2
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogonodaeAndropogoneaeTripsacinaeZea
Proteomesi
  • UP000007305 Componenti: Chromosome 6

Organism-specific databases

MaizeGDBi131629.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 768768DNA replication licensing factor MCM3 homolog 2PRO_0000292945Add
BLAST

Proteomic databases

PaxDbiQ9SX04.
PRIDEiQ9SX04.

Expressioni

Gene expression databases

ExpressionAtlasiQ9SX04. baseline and differential.
GenevisibleiQ9SX04. ZM.

Interactioni

Protein-protein interaction databases

STRINGi4577.GRMZM2G100639_P01.

Structurei

3D structure databases

ProteinModelPortaliQ9SX04.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini290 – 497208MCMAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi472 – 4754Arginine finger

Sequence similaritiesi

Belongs to the MCM family.Curated
Contains 1 MCM domain.Curated

Phylogenomic databases

eggNOGiKOG0479. Eukaryota.
COG1241. LUCA.
KOiK02541.
OMAiLTIGMDD.

Family and domain databases

Gene3Di2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR031327. MCM.
IPR008046. Mcm3.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01659. MCMPROTEIN3.
SMARTiSM00382. AAA. 1 hit.
SM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SX04-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEINEEAMAA HKRAFLDFLD QDVGKGVYMQ AVRDMVQNKR HRLIIGMDDL
60 70 80 90 100
RNHNLDLARR VIRTPGEYMQ PASDAVSEVA RNLDPKFLKE GERVMVGFSG
110 120 130 140 150
PFGFHRVTPR DLMSSFIGTM VCVEGIVTKC SLVRPKVVKS VHFCPVTGDF
160 170 180 190 200
LSREYRDITS FVGLPTGSVY PTRDDNGNLL VTEYGMCEYK DHQTLSMQEV
210 220 230 240 250
PENSAPGQLP RTVDVIVEDD LVDCCKPGDR VSIVGVYKAL PGKSKGSVSG
260 270 280 290 300
VFRTVLIANN VSLLNKEANA PVYTREDLKR MKEISRRNDT FDLLGNSLAP
310 320 330 340 350
SIYGHLWIKK AVVLLMLGGV EKNLKNGTHL RGDINMMMVG DPSVAKSQLL
360 370 380 390 400
RAVMNIAPLA ISTTGRGSSG VGLTAAVTSD QETGERRLEA GAMVLADRGV
410 420 430 440 450
VCIDEFDKMN DQDRVAIHEV MEQQTVTIAK AGIHASLNAR CSVIAAANPI
460 470 480 490 500
YGTYDRSLTP TKNIGLPDSL LSRFDLLFIV LDQMDPEIDR QISEHVARMH
510 520 530 540 550
RYCTDDGGAR SLDKEGYAEE DDGDANAAIF VKYDRMLHGQ DRRRGKKSKQ
560 570 580 590 600
DRLTVKFLKK YIHYAKNLIQ PRLTDEASDH IATSYAELRD GSANAKSGGG
610 620 630 640 650
TLPITARTLE TIIRLSTAHA KMKLRHEVLK SDVEAALQVL NFAIYHKELT
660 670 680 690 700
EMEEREQREM EMKQQADHDA GATGGTVDGH GSSGNDPMDV DVGSNDQNVS
710 720 730 740 750
AERIEAFEAL LGQHVLANHI DQMSIDDIEQ MVNRESTAPY TRSQVEFILE
760
RMQDANRVMI RDGVVRII
Length:768
Mass (Da):85,196
Last modified:May 1, 2000 - v1
Checksum:iA82ACAC0BD7B64E0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF073330 mRNA. Translation: AAD48086.1.
RefSeqiNP_001105718.1. NM_001112248.2.
UniGeneiZm.138412.
Zm.174.

Genome annotation databases

EnsemblPlantsiGRMZM2G100639_T01; GRMZM2G100639_P01; GRMZM2G100639.
GeneIDi542738.
GrameneiGRMZM2G100639_T01; GRMZM2G100639_P01; GRMZM2G100639.
KEGGizma:542738.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF073330 mRNA. Translation: AAD48086.1.
RefSeqiNP_001105718.1. NM_001112248.2.
UniGeneiZm.138412.
Zm.174.

3D structure databases

ProteinModelPortaliQ9SX04.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4577.GRMZM2G100639_P01.

Proteomic databases

PaxDbiQ9SX04.
PRIDEiQ9SX04.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiGRMZM2G100639_T01; GRMZM2G100639_P01; GRMZM2G100639.
GeneIDi542738.
GrameneiGRMZM2G100639_T01; GRMZM2G100639_P01; GRMZM2G100639.
KEGGizma:542738.

Organism-specific databases

MaizeGDBi131629.

Phylogenomic databases

eggNOGiKOG0479. Eukaryota.
COG1241. LUCA.
KOiK02541.
OMAiLTIGMDD.

Gene expression databases

ExpressionAtlasiQ9SX04. baseline and differential.
GenevisibleiQ9SX04. ZM.

Family and domain databases

Gene3Di2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR031327. MCM.
IPR008046. Mcm3.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01659. MCMPROTEIN3.
SMARTiSM00382. AAA. 1 hit.
SM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "cDNA and promoter sequences for MCM3 homologues from maize, and protein localization in cycling cells."
    Sabelli P.A., Parker J.S., Barlow P.W.
    J. Exp. Bot. 50:1315-1322(1999)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. LG11.
    Tissue: Root.

Entry informationi

Entry nameiMCM32_MAIZE
AccessioniPrimary (citable) accession number: Q9SX04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 12, 2007
Last sequence update: May 1, 2000
Last modified: June 8, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.