Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9SWW5 (PCS1_WHEAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione gamma-glutamylcysteinyltransferase 1

EC=2.3.2.15
Alternative name(s):
Cadmium tolerance protein
Phytochelatin synthase 1
TaPCS1
Gene names
Name:PCS1
OrganismTriticum aestivum (Wheat) [Complete proteome]
Taxonomic identifier4565 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeTriticum

Protein attributes

Sequence length500 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in the synthesis of phytochelatins (PC) and homophytochelatins (hPC), the heavy-metal-binding peptides of plants. Ref.1

Catalytic activity

Glutathione + (Glu(-Cys))(n)-Gly = Gly + (Glu(-Cys))(n+1)-Gly.

Enzyme regulation

Requires cadmium for activity By similarity.

Tissue specificity

Expressed in roots and shoots. Ref.1

Induction

5- to 10-fold by 100 µM cadmium. Ref.1

Sequence similarities

Belongs to the phytochelatin synthase family.

Contains 1 peptidase C83 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 500500Glutathione gamma-glutamylcysteinyltransferase 1
PRO_0000287212

Regions

Domain1 – 221221Peptidase C83
Compositional bias333 – 3386Poly-Ser

Sites

Active site561 By similarity
Active site1621 By similarity
Active site1801 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9SWW5 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 530B8B55FC46B008

FASTA50055,045
        10         20         30         40         50         60 
MEVASLYRRV LPSPPAVEFA SAEGKRLFAE ALQGGTMEGF FNLISYFQTQ SEPAFCGLAS 

        70         80         90        100        110        120 
LSVVLNALAI DPGRPWKGPW RWFDESMLDC CEPLHKVKAE GITFGKVVCL AHCAGARVQS 

       130        140        150        160        170        180 
FRADQTTIHD FRAHLTRCAS SQDCHLISSY HRSPFKQTGT GHFSPIGGYH AEKDMALILD 

       190        200        210        220        230        240 
VARFKYPPHW VPLTLLWDAM NTTDEATGLL RGFMLVSRRS SAPSLLYTVS CGHGSWKSMA 

       250        260        270        280        290        300 
KYCVEDVPNL LKDESLDNVT TLLSRLVESL PANAGDLIKC VIEVRRKEEG ESSLSKEEKE 

       310        320        330        340        350        360 
RLFLKEKVLQ QIRDTDLFRV VHELQYPKGL CGSCSSSSDE DSLAEIAATV CCQGAAFLSG 

       370        380        390        400        410        420 
NLVSRDGFCC RETCIKCIEA NGDGLKTVIS GTVVSKGNEQ AVDLLLPTSS SKTSLCNSNL 

       430        440        450        460        470        480 
KSKIVKYPSS TDVLTVLLLV LQPNTWLGIK DENVKAEFQS LVSTDNLPDL LKQEILHLRR 

       490        500 
QLHYLAGCKG QEACQEPPSP 

« Hide

References

[1]"Tolerance to toxic metals by a gene family of phytochelatin synthases from plants and yeast."
Clemens S., Kim E.J., Neumann D., Schroeder J.I.
EMBO J. 18:3325-3333(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION BY CADMIUM, TISSUE SPECIFICITY.
Tissue: Root.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF093752 mRNA. Translation: AAD50592.1.
UniGeneTa.232.

3D structure databases

ProteinModelPortalQ9SWW5.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSC83.002.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneQ9SWW5.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-16321.

Family and domain databases

InterProIPR007719. Phytochelatin_synthase.
IPR015407. Phytochelatin_synthase_C.
[Graphical view]
PfamPF05023. Phytochelatin. 1 hit.
PF09328. Phytochelatin_C. 1 hit.
[Graphical view]
PROSITEPS51443. PCS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePCS1_WHEAT
AccessionPrimary (citable) accession number: Q9SWW5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 1, 2000
Last modified: April 16, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries