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Q9SWW5

- PCS1_WHEAT

UniProt

Q9SWW5 - PCS1_WHEAT

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Protein

Glutathione gamma-glutamylcysteinyltransferase 1

Gene

PCS1

Organism
Triticum aestivum (Wheat)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Involved in the synthesis of phytochelatins (PC) and homophytochelatins (hPC), the heavy-metal-binding peptides of plants.1 Publication

Catalytic activityi

Glutathione + (Glu(-Cys))(n)-Gly = Gly + (Glu(-Cys))(n+1)-Gly.PROSITE-ProRule annotation

Enzyme regulationi

Requires cadmium for activity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei56 – 561PROSITE-ProRule annotation
Active sitei162 – 1621PROSITE-ProRule annotation
Active sitei180 – 1801PROSITE-ProRule annotation

GO - Molecular functioni

  1. glutathione gamma-glutamylcysteinyltransferase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. phytochelatin biosynthetic process Source: InterPro
  2. response to metal ion Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Ligandi

Cadmium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16321.

Protein family/group databases

MEROPSiC83.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione gamma-glutamylcysteinyltransferase 1 (EC:2.3.2.15)
Alternative name(s):
Cadmium tolerance protein
Phytochelatin synthase 1
TaPCS1
Gene namesi
Name:PCS1
OrganismiTriticum aestivum (Wheat)
Taxonomic identifieri4565 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeTriticum
ProteomesiUP000019116: Unplaced

Organism-specific databases

GrameneiQ9SWW5.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 500500Glutathione gamma-glutamylcysteinyltransferase 1PRO_0000287212Add
BLAST

Expressioni

Tissue specificityi

Expressed in roots and shoots.1 Publication

Inductioni

5- to 10-fold by 100 µM cadmium.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ9SWW5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 221221Peptidase C83PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi333 – 3386Poly-Ser

Sequence similaritiesi

Belongs to the phytochelatin synthase family.PROSITE-ProRule annotation
Contains 1 peptidase C83 domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR007719. Phytochelatin_synthase.
IPR015407. Phytochelatin_synthase_C.
[Graphical view]
PfamiPF05023. Phytochelatin. 1 hit.
PF09328. Phytochelatin_C. 1 hit.
[Graphical view]
PROSITEiPS51443. PCS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SWW5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEVASLYRRV LPSPPAVEFA SAEGKRLFAE ALQGGTMEGF FNLISYFQTQ
60 70 80 90 100
SEPAFCGLAS LSVVLNALAI DPGRPWKGPW RWFDESMLDC CEPLHKVKAE
110 120 130 140 150
GITFGKVVCL AHCAGARVQS FRADQTTIHD FRAHLTRCAS SQDCHLISSY
160 170 180 190 200
HRSPFKQTGT GHFSPIGGYH AEKDMALILD VARFKYPPHW VPLTLLWDAM
210 220 230 240 250
NTTDEATGLL RGFMLVSRRS SAPSLLYTVS CGHGSWKSMA KYCVEDVPNL
260 270 280 290 300
LKDESLDNVT TLLSRLVESL PANAGDLIKC VIEVRRKEEG ESSLSKEEKE
310 320 330 340 350
RLFLKEKVLQ QIRDTDLFRV VHELQYPKGL CGSCSSSSDE DSLAEIAATV
360 370 380 390 400
CCQGAAFLSG NLVSRDGFCC RETCIKCIEA NGDGLKTVIS GTVVSKGNEQ
410 420 430 440 450
AVDLLLPTSS SKTSLCNSNL KSKIVKYPSS TDVLTVLLLV LQPNTWLGIK
460 470 480 490 500
DENVKAEFQS LVSTDNLPDL LKQEILHLRR QLHYLAGCKG QEACQEPPSP
Length:500
Mass (Da):55,045
Last modified:May 1, 2000 - v1
Checksum:i530B8B55FC46B008
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF093752 mRNA. Translation: AAD50592.1.
UniGeneiTa.232.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF093752 mRNA. Translation: AAD50592.1 .
UniGenei Ta.232.

3D structure databases

ProteinModelPortali Q9SWW5.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C83.002.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

Gramenei Q9SWW5.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-16321.

Family and domain databases

InterProi IPR007719. Phytochelatin_synthase.
IPR015407. Phytochelatin_synthase_C.
[Graphical view ]
Pfami PF05023. Phytochelatin. 1 hit.
PF09328. Phytochelatin_C. 1 hit.
[Graphical view ]
PROSITEi PS51443. PCS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Tolerance to toxic metals by a gene family of phytochelatin synthases from plants and yeast."
    Clemens S., Kim E.J., Neumann D., Schroeder J.I.
    EMBO J. 18:3325-3333(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION BY CADMIUM, TISSUE SPECIFICITY.
    Tissue: Root.

Entry informationi

Entry nameiPCS1_WHEAT
AccessioniPrimary (citable) accession number: Q9SWW5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 1, 2000
Last modified: October 1, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3