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Q9SWW5

- PCS1_WHEAT

UniProt

Q9SWW5 - PCS1_WHEAT

Protein

Glutathione gamma-glutamylcysteinyltransferase 1

Gene

PCS1

Organism
Triticum aestivum (Wheat)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Involved in the synthesis of phytochelatins (PC) and homophytochelatins (hPC), the heavy-metal-binding peptides of plants.1 Publication

    Catalytic activityi

    Glutathione + (Glu(-Cys))(n)-Gly = Gly + (Glu(-Cys))(n+1)-Gly.PROSITE-ProRule annotation

    Enzyme regulationi

    Requires cadmium for activity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei56 – 561PROSITE-ProRule annotation
    Active sitei162 – 1621PROSITE-ProRule annotation
    Active sitei180 – 1801PROSITE-ProRule annotation

    GO - Molecular functioni

    1. glutathione gamma-glutamylcysteinyltransferase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. phytochelatin biosynthetic process Source: InterPro
    2. response to metal ion Source: InterPro

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Ligandi

    Cadmium, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-16321.

    Protein family/group databases

    MEROPSiC83.002.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione gamma-glutamylcysteinyltransferase 1 (EC:2.3.2.15)
    Alternative name(s):
    Cadmium tolerance protein
    Phytochelatin synthase 1
    TaPCS1
    Gene namesi
    Name:PCS1
    OrganismiTriticum aestivum (Wheat)
    Taxonomic identifieri4565 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeTriticum
    ProteomesiUP000019116: Unplaced

    Organism-specific databases

    GrameneiQ9SWW5.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 500500Glutathione gamma-glutamylcysteinyltransferase 1PRO_0000287212Add
    BLAST

    Expressioni

    Tissue specificityi

    Expressed in roots and shoots.1 Publication

    Inductioni

    5- to 10-fold by 100 µM cadmium.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SWW5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 221221Peptidase C83PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi333 – 3386Poly-Ser

    Sequence similaritiesi

    Belongs to the phytochelatin synthase family.PROSITE-ProRule annotation
    Contains 1 peptidase C83 domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR007719. Phytochelatin_synthase.
    IPR015407. Phytochelatin_synthase_C.
    [Graphical view]
    PfamiPF05023. Phytochelatin. 1 hit.
    PF09328. Phytochelatin_C. 1 hit.
    [Graphical view]
    PROSITEiPS51443. PCS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9SWW5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEVASLYRRV LPSPPAVEFA SAEGKRLFAE ALQGGTMEGF FNLISYFQTQ    50
    SEPAFCGLAS LSVVLNALAI DPGRPWKGPW RWFDESMLDC CEPLHKVKAE 100
    GITFGKVVCL AHCAGARVQS FRADQTTIHD FRAHLTRCAS SQDCHLISSY 150
    HRSPFKQTGT GHFSPIGGYH AEKDMALILD VARFKYPPHW VPLTLLWDAM 200
    NTTDEATGLL RGFMLVSRRS SAPSLLYTVS CGHGSWKSMA KYCVEDVPNL 250
    LKDESLDNVT TLLSRLVESL PANAGDLIKC VIEVRRKEEG ESSLSKEEKE 300
    RLFLKEKVLQ QIRDTDLFRV VHELQYPKGL CGSCSSSSDE DSLAEIAATV 350
    CCQGAAFLSG NLVSRDGFCC RETCIKCIEA NGDGLKTVIS GTVVSKGNEQ 400
    AVDLLLPTSS SKTSLCNSNL KSKIVKYPSS TDVLTVLLLV LQPNTWLGIK 450
    DENVKAEFQS LVSTDNLPDL LKQEILHLRR QLHYLAGCKG QEACQEPPSP 500
    Length:500
    Mass (Da):55,045
    Last modified:May 1, 2000 - v1
    Checksum:i530B8B55FC46B008
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF093752 mRNA. Translation: AAD50592.1.
    UniGeneiTa.232.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF093752 mRNA. Translation: AAD50592.1 .
    UniGenei Ta.232.

    3D structure databases

    ProteinModelPortali Q9SWW5.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C83.002.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    Gramenei Q9SWW5.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-16321.

    Family and domain databases

    InterProi IPR007719. Phytochelatin_synthase.
    IPR015407. Phytochelatin_synthase_C.
    [Graphical view ]
    Pfami PF05023. Phytochelatin. 1 hit.
    PF09328. Phytochelatin_C. 1 hit.
    [Graphical view ]
    PROSITEi PS51443. PCS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Tolerance to toxic metals by a gene family of phytochelatin synthases from plants and yeast."
      Clemens S., Kim E.J., Neumann D., Schroeder J.I.
      EMBO J. 18:3325-3333(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION BY CADMIUM, TISSUE SPECIFICITY.
      Tissue: Root.

    Entry informationi

    Entry nameiPCS1_WHEAT
    AccessioniPrimary (citable) accession number: Q9SWW5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 15, 2007
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3