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Protein

Probable transmembrane ascorbate ferrireductase 2

Gene

CYB561B

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Two-heme-containing cytochrome. Catalyzes ascorbate-dependent trans-membrane ferric-chelate reduction (By similarity).By similarity

Catalytic activityi

Ascorbate(Side 1) + Fe(III)(Side 2) = monodehydroascorbate(Side 1) + Fe(II)(Side 2).

Cofactori

hemeBy similarityNote: Binds 2 heme groups non-covalently.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi51Iron (heme axial ligand); via tele nitrogen1 Publication1
Binding sitei77Ascorbate1 Publication1
Binding sitei81Ascorbate1 Publication1
Metal bindingi84Iron (heme axial ligand); via tele nitrogen1 Publication1
Binding sitei105Monodehydroascorbate1 Publication1
Binding sitei106Monodehydroascorbate1 Publication1
Binding sitei115Monodehydroascorbate1 Publication1
Metal bindingi118Iron (heme axial ligand); via tele nitrogen1 Publication1
Binding sitei140Ascorbate1 Publication1
Binding sitei150Ascorbate1 Publication1
Binding sitei151Ascorbate; via amide nitrogen1 Publication1
Metal bindingi157Iron (heme axial ligand); via tele nitrogen1 Publication1
Binding sitei182Monodehydroascorbate1 Publication1
Binding sitei186Monodehydroascorbate1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BRENDAi1.16.5.1. 399.
ReactomeiR-ATH-917937. Iron uptake and transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable transmembrane ascorbate ferrireductase 2 (EC:1.16.5.1)
Alternative name(s):
Cytochrome b561-1
Short name:
Artb561-2
Short name:
AtCytb561
Protein b561A.tha4
Gene namesi
Name:CYB561B
Synonyms:ACYB-1, CYB-1, CYB561B2, CYBASC2, CYTB561
Ordered Locus Names:At5g38630
ORF Names:MBB18.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G38630.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei5 – 25Helical; Name=1Sequence analysisAdd BLAST21
Transmembranei50 – 70Helical; Name=2Sequence analysisAdd BLAST21
Transmembranei82 – 102Helical; Name=3Sequence analysisAdd BLAST21
Transmembranei120 – 140Helical; Name=4Sequence analysisAdd BLAST21
Transmembranei157 – 177Helical; Name=5Sequence analysisAdd BLAST21
Transmembranei198 – 218Helical; Name=6Sequence analysisAdd BLAST21

GO - Cellular componenti

  • Golgi apparatus Source: TAIR
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi81K → A: Abrogates electron transfer; when associated with W-105/E-106/A-150. 1 Publication1
Mutagenesisi105F → W: Abrogates electron transfer; when associated with A-81/E-106/A-150. 1 Publication1
Mutagenesisi106H → E: Abrogates electron transfer; when associated with A-81/W-105/A-150. 1 Publication1
Mutagenesisi150R → A: Abrogates electron transfer; when associated with A-81/W-105/E-106. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004129081 – 230Probable transmembrane ascorbate ferrireductase 2Add BLAST230

Proteomic databases

PaxDbiQ9SWS1.

Expressioni

Tissue specificityi

Expressed in roots, seedlings, leaves and flowers. Expressed in the L1 layer of the shoot apex, in the epidermis of leaf primordia and young leaves and in vascular bundles. In the differentiation zone of the root, detected in the pericycle and in the epidermis, but not in the cortex. Strongly expressed in the cortical region of the root tip, in the meristematic tissue and in the epidermal cell layer of lateral roots, but not in the root caps. Highly expressed in unfertilized ovules. In mature embryos, expressed in the epidermis, cotyledon tips and root tips.1 Publication

Developmental stagei

Strong redution in expression levels in flowers following fertilization.1 Publication

Gene expression databases

GenevisibleiQ9SWS1. AT.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi3702.AT5G38630.1.

Structurei

Secondary structure

1230
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi11 – 31Combined sources21
Helixi44 – 59Combined sources16
Helixi61 – 67Combined sources7
Helixi69 – 72Combined sources4
Helixi77 – 109Combined sources33
Helixi117 – 140Combined sources24
Helixi147 – 185Combined sources39
Helixi194 – 218Combined sources25

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4O6YX-ray1.70A/B1-230[»]
4O79X-ray2.00A/B1-230[»]
4O7GX-ray2.21A/B1-230[»]
ProteinModelPortaliQ9SWS1.
SMRiQ9SWS1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 218Cytochrome b561PROSITE-ProRule annotationAdd BLAST205

Sequence similaritiesi

Contains 1 cytochrome b561 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1619. Eukaryota.
ENOG4111FKI. LUCA.
HOGENOMiHOG000231044.
InParanoidiQ9SWS1.
KOiK08360.
OMAiPAAMENF.
OrthoDBiEOG09360NBB.
PhylomeDBiQ9SWS1.

Family and domain databases

InterProiIPR006593. Cyt_b561/ferric_Rdtase_TM.
[Graphical view]
PfamiPF03188. Cytochrom_B561. 1 hit.
[Graphical view]
SMARTiSM00665. B561. 1 hit.
[Graphical view]
PROSITEiPS50939. CYTOCHROME_B561. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SWS1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVPVLGGFP IFMVVRVLGF IIAALVLTWT VHYRGGLALS SDNKDHIFNV
60 70 80 90 100
HPVMMVIGLI LFNGEAMLAY KSVQGTKNLK KLVHLTLQLT AFILSLIGVW
110 120 130 140 150
AALKFHIDKG IENFYSLHSW LGLACLFLFA FQWAAGFVTY WYPGGSRNSR
160 170 180 190 200
ASLMPWHVFL GISIYALALV TATTGILEKV TFLQVNQVIT RYSTEAMLVN
210 220 230
TMGVLILILG GFVILGVVTP VSGKDQVLTQ
Length:230
Mass (Da):25,289
Last modified:May 1, 2000 - v1
Checksum:i544F69F682C9D259
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF132115 Genomic DNA. Translation: AAD45585.1.
AB049627 mRNA. Translation: BAB21521.1.
AB005231 Genomic DNA. Translation: BAB10153.1.
CP002688 Genomic DNA. Translation: AED94343.1.
BT024611 mRNA. Translation: ABD43009.1.
AY085028 mRNA. Translation: AAM61586.1.
AK117270 mRNA. Translation: BAC41943.1.
Z26702 mRNA. Translation: CAA81401.1.
RefSeqiNP_198679.1. NM_123224.3.
UniGeneiAt.49099.

Genome annotation databases

EnsemblPlantsiAT5G38630.1; AT5G38630.1; AT5G38630.
GeneIDi833853.
GrameneiAT5G38630.1; AT5G38630.1; AT5G38630.
KEGGiath:AT5G38630.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF132115 Genomic DNA. Translation: AAD45585.1.
AB049627 mRNA. Translation: BAB21521.1.
AB005231 Genomic DNA. Translation: BAB10153.1.
CP002688 Genomic DNA. Translation: AED94343.1.
BT024611 mRNA. Translation: ABD43009.1.
AY085028 mRNA. Translation: AAM61586.1.
AK117270 mRNA. Translation: BAC41943.1.
Z26702 mRNA. Translation: CAA81401.1.
RefSeqiNP_198679.1. NM_123224.3.
UniGeneiAt.49099.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4O6YX-ray1.70A/B1-230[»]
4O79X-ray2.00A/B1-230[»]
4O7GX-ray2.21A/B1-230[»]
ProteinModelPortaliQ9SWS1.
SMRiQ9SWS1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G38630.1.

Proteomic databases

PaxDbiQ9SWS1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G38630.1; AT5G38630.1; AT5G38630.
GeneIDi833853.
GrameneiAT5G38630.1; AT5G38630.1; AT5G38630.
KEGGiath:AT5G38630.

Organism-specific databases

TAIRiAT5G38630.

Phylogenomic databases

eggNOGiKOG1619. Eukaryota.
ENOG4111FKI. LUCA.
HOGENOMiHOG000231044.
InParanoidiQ9SWS1.
KOiK08360.
OMAiPAAMENF.
OrthoDBiEOG09360NBB.
PhylomeDBiQ9SWS1.

Enzyme and pathway databases

BRENDAi1.16.5.1. 399.
ReactomeiR-ATH-917937. Iron uptake and transport.

Miscellaneous databases

PROiQ9SWS1.

Gene expression databases

GenevisibleiQ9SWS1. AT.

Family and domain databases

InterProiIPR006593. Cyt_b561/ferric_Rdtase_TM.
[Graphical view]
PfamiPF03188. Cytochrom_B561. 1 hit.
[Graphical view]
SMARTiSM00665. B561. 1 hit.
[Graphical view]
PROSITEiPS50939. CYTOCHROME_B561. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACFR2_ARATH
AccessioniPrimary (citable) accession number: Q9SWS1
Secondary accession number(s): Q42137
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: May 1, 2000
Last modified: November 2, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.