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Protein

Probable transmembrane ascorbate ferrireductase 2

Gene

CYB561B

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Two-heme-containing cytochrome. Catalyzes ascorbate-dependent trans-membrane ferric-chelate reduction (By similarity).By similarity

Catalytic activityi

Ascorbate(Side 1) + Fe(III)(Side 2) = monodehydroascorbate(Side 1) + Fe(II)(Side 2).

Cofactori

hemeBy similarityNote: Binds 2 heme groups non-covalently.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi51 – 511Iron (heme axial ligand); via tele nitrogen1 Publication
Binding sitei77 – 771Ascorbate1 Publication
Binding sitei81 – 811Ascorbate1 Publication
Metal bindingi84 – 841Iron (heme axial ligand); via tele nitrogen1 Publication
Binding sitei105 – 1051Monodehydroascorbate1 Publication
Binding sitei106 – 1061Monodehydroascorbate1 Publication
Binding sitei115 – 1151Monodehydroascorbate1 Publication
Metal bindingi118 – 1181Iron (heme axial ligand); via tele nitrogen1 Publication
Binding sitei140 – 1401Ascorbate1 Publication
Binding sitei150 – 1501Ascorbate1 Publication
Binding sitei151 – 1511Ascorbate; via amide nitrogen1 Publication
Metal bindingi157 – 1571Iron (heme axial ligand); via tele nitrogen1 Publication
Binding sitei182 – 1821Monodehydroascorbate1 Publication
Binding sitei186 – 1861Monodehydroascorbate1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BRENDAi1.16.5.1. 399.
ReactomeiR-ATH-917937. Iron uptake and transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable transmembrane ascorbate ferrireductase 2 (EC:1.16.5.1)
Alternative name(s):
Cytochrome b561-1
Short name:
Artb561-2
Short name:
AtCytb561
Protein b561A.tha4
Gene namesi
Name:CYB561B
Synonyms:ACYB-1, CYB-1, CYB561B2, CYBASC2, CYTB561
Ordered Locus Names:At5g38630
ORF Names:MBB18.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G38630.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei5 – 2521Helical; Name=1Sequence analysisAdd
BLAST
Transmembranei50 – 7021Helical; Name=2Sequence analysisAdd
BLAST
Transmembranei82 – 10221Helical; Name=3Sequence analysisAdd
BLAST
Transmembranei120 – 14021Helical; Name=4Sequence analysisAdd
BLAST
Transmembranei157 – 17721Helical; Name=5Sequence analysisAdd
BLAST
Transmembranei198 – 21821Helical; Name=6Sequence analysisAdd
BLAST

GO - Cellular componenti

  • Golgi apparatus Source: TAIR
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi81 – 811K → A: Abrogates electron transfer; when associated with W-105/E-106/A-150. 1 Publication
Mutagenesisi105 – 1051F → W: Abrogates electron transfer; when associated with A-81/E-106/A-150. 1 Publication
Mutagenesisi106 – 1061H → E: Abrogates electron transfer; when associated with A-81/W-105/A-150. 1 Publication
Mutagenesisi150 – 1501R → A: Abrogates electron transfer; when associated with A-81/W-105/E-106. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 230230Probable transmembrane ascorbate ferrireductase 2PRO_0000412908Add
BLAST

Proteomic databases

PaxDbiQ9SWS1.
PRIDEiQ9SWS1.

Expressioni

Tissue specificityi

Expressed in roots, seedlings, leaves and flowers. Expressed in the L1 layer of the shoot apex, in the epidermis of leaf primordia and young leaves and in vascular bundles. In the differentiation zone of the root, detected in the pericycle and in the epidermis, but not in the cortex. Strongly expressed in the cortical region of the root tip, in the meristematic tissue and in the epidermal cell layer of lateral roots, but not in the root caps. Highly expressed in unfertilized ovules. In mature embryos, expressed in the epidermis, cotyledon tips and root tips.1 Publication

Developmental stagei

Strong redution in expression levels in flowers following fertilization.1 Publication

Gene expression databases

GenevisibleiQ9SWS1. AT.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi3702.AT5G38630.1.

Structurei

Secondary structure

1
230
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi11 – 3121Combined sources
Helixi44 – 5916Combined sources
Helixi61 – 677Combined sources
Helixi69 – 724Combined sources
Helixi77 – 10933Combined sources
Helixi117 – 14024Combined sources
Helixi147 – 18539Combined sources
Helixi194 – 21825Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4O6YX-ray1.70A/B1-230[»]
4O79X-ray2.00A/B1-230[»]
4O7GX-ray2.21A/B1-230[»]
ProteinModelPortaliQ9SWS1.
SMRiQ9SWS1. Positions 9-219.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 218205Cytochrome b561PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 cytochrome b561 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1619. Eukaryota.
ENOG4111FKI. LUCA.
HOGENOMiHOG000231044.
InParanoidiQ9SWS1.
KOiK08360.
OMAiTEGIVIN.
PhylomeDBiQ9SWS1.

Family and domain databases

InterProiIPR006593. Cyt_b561/ferric_Rdtase_TM.
[Graphical view]
PfamiPF03188. Cytochrom_B561. 1 hit.
[Graphical view]
SMARTiSM00665. B561. 1 hit.
[Graphical view]
PROSITEiPS50939. CYTOCHROME_B561. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SWS1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVPVLGGFP IFMVVRVLGF IIAALVLTWT VHYRGGLALS SDNKDHIFNV
60 70 80 90 100
HPVMMVIGLI LFNGEAMLAY KSVQGTKNLK KLVHLTLQLT AFILSLIGVW
110 120 130 140 150
AALKFHIDKG IENFYSLHSW LGLACLFLFA FQWAAGFVTY WYPGGSRNSR
160 170 180 190 200
ASLMPWHVFL GISIYALALV TATTGILEKV TFLQVNQVIT RYSTEAMLVN
210 220 230
TMGVLILILG GFVILGVVTP VSGKDQVLTQ
Length:230
Mass (Da):25,289
Last modified:May 1, 2000 - v1
Checksum:i544F69F682C9D259
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF132115 Genomic DNA. Translation: AAD45585.1.
AB049627 mRNA. Translation: BAB21521.1.
AB005231 Genomic DNA. Translation: BAB10153.1.
CP002688 Genomic DNA. Translation: AED94343.1.
BT024611 mRNA. Translation: ABD43009.1.
AY085028 mRNA. Translation: AAM61586.1.
AK117270 mRNA. Translation: BAC41943.1.
Z26702 mRNA. Translation: CAA81401.1.
RefSeqiNP_198679.1. NM_123224.3.
UniGeneiAt.49099.

Genome annotation databases

EnsemblPlantsiAT5G38630.1; AT5G38630.1; AT5G38630.
GeneIDi833853.
GrameneiAT5G38630.1; AT5G38630.1; AT5G38630.
KEGGiath:AT5G38630.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF132115 Genomic DNA. Translation: AAD45585.1.
AB049627 mRNA. Translation: BAB21521.1.
AB005231 Genomic DNA. Translation: BAB10153.1.
CP002688 Genomic DNA. Translation: AED94343.1.
BT024611 mRNA. Translation: ABD43009.1.
AY085028 mRNA. Translation: AAM61586.1.
AK117270 mRNA. Translation: BAC41943.1.
Z26702 mRNA. Translation: CAA81401.1.
RefSeqiNP_198679.1. NM_123224.3.
UniGeneiAt.49099.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4O6YX-ray1.70A/B1-230[»]
4O79X-ray2.00A/B1-230[»]
4O7GX-ray2.21A/B1-230[»]
ProteinModelPortaliQ9SWS1.
SMRiQ9SWS1. Positions 9-219.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G38630.1.

Proteomic databases

PaxDbiQ9SWS1.
PRIDEiQ9SWS1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G38630.1; AT5G38630.1; AT5G38630.
GeneIDi833853.
GrameneiAT5G38630.1; AT5G38630.1; AT5G38630.
KEGGiath:AT5G38630.

Organism-specific databases

TAIRiAT5G38630.

Phylogenomic databases

eggNOGiKOG1619. Eukaryota.
ENOG4111FKI. LUCA.
HOGENOMiHOG000231044.
InParanoidiQ9SWS1.
KOiK08360.
OMAiTEGIVIN.
PhylomeDBiQ9SWS1.

Enzyme and pathway databases

BRENDAi1.16.5.1. 399.
ReactomeiR-ATH-917937. Iron uptake and transport.

Miscellaneous databases

PROiQ9SWS1.

Gene expression databases

GenevisibleiQ9SWS1. AT.

Family and domain databases

InterProiIPR006593. Cyt_b561/ferric_Rdtase_TM.
[Graphical view]
PfamiPF03188. Cytochrom_B561. 1 hit.
[Graphical view]
SMARTiSM00665. B561. 1 hit.
[Graphical view]
PROSITEiPS50939. CYTOCHROME_B561. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Arabidopsis thaliana sequence analysis confirms the presence of cyt b-561 in plants: Evidence for a novel protein family."
    Asard H., Terol-Alcayde J., Preger V., Del Favero J., Verelst W., Sparla F., Perez-Alonso M., Trost P.
    Plant Physiol. Biochem. 38:905-912(2000)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Molecular cloning of cytochrome b561 from Arabidopsis thaliana."
    Asada A., Kusakawa T., Orii H., Watanabe K., Tsubaki M.
    Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
    Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
    DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Arabidopsis ORF clones."
    Shinn P., Chen H., Kim C.J., Ecker J.R.
    Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  8. "The Arabidopsis thaliana transcribed genome: the GDR cDNA program."
    Desprez T., Amselem J., Chiapello H., Caboche M., Hofte H.
    Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-75.
    Strain: cv. Columbia.
    Tissue: Seedling.
  9. "Higher-plant plasma membrane cytochrome b561: a protein in search of a function."
    Asard H., Kapila J., Verelst W., Berczi A.
    Protoplasma 217:77-93(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  10. "Tissue-specific expression and developmental regulation of cytochrome b561 genes in Arabidopsis thaliana and Raphanus sativus."
    Verelst W., Kapila J., De Almeida Engler J., Stone J.M., Caubergs R., Asard H.
    Physiol. Plantarum 120:312-318(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  11. "Cytochrome b561 protein family: expanding roles and versatile transmembrane electron transfer abilities as predicted by a new classification system and protein sequence motif analyses."
    Tsubaki M., Takeuchi F., Nakanishi N.
    Biochim. Biophys. Acta 1753:174-190(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  12. "Cytochromes b561: ascorbate-mediated trans-membrane electron transport."
    Asard H., Barbaro R., Trost P., Berczi A.
    Antioxid. Redox Signal. 19:1026-1035(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  13. "Structure and mechanism of a eukaryotic transmembrane ascorbate-dependent oxidoreductase."
    Lu P., Ma D., Yan C., Gong X., Du M., Shi Y.
    Proc. Natl. Acad. Sci. U.S.A. 111:1813-1818(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) IN COMPLEX WITH ASCORBATE; MONODEHYDROASCORBATE AND HEME, SUBUNIT, MUTAGENESIS OF LYS-81; PHE-105; HIS-106 AND ARG-150.

Entry informationi

Entry nameiACFR2_ARATH
AccessioniPrimary (citable) accession number: Q9SWS1
Secondary accession number(s): Q42137
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: May 1, 2000
Last modified: May 11, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.