Reviewed, UniProtKB/Swiss-Prot Q9SWI1 (PKS1_ARATH)

Names and origin

Protein names

Recommended name:
    Phytochrome kinase substrate 1

Gene names

Name:	PKS1
Ordered Locus Names:	At2g02950
ORF Names:	T17M13.12

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	439 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May be responsible for light-regulated cytoplasmic sequestration of phytochromes or may be a negative regulator of phytochrome B signaling.
Subunit structure	Interacts with the C-termini of both phytochromes A (phyA) and B (phyB). Binds both spectral forms of phytochrome, Pr and Pfr.
Subcellular location	Cytoplasm.
Tissue specificity	Expressed in young seedlings in both darkness and light. Moderate in leaves and very low in roots and flowers.
Developmental stage	Decreases with development.
Post-translational modification	Phosphorylated on Ser and to a lower extent on Thr by phytochromes. Phosphorylation is stimulated twofold by red light.

Ontologies

Keywords
Biological process	Phytochrome signaling pathway
Cellular component	Cytoplasm
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phototropism Inferred from genetic interaction. Source: TAIR positive gravitropism Inferred from mutant phenotype. Source: TAIR red or far red light signaling pathway Inferred from mutant phenotype. Source: TAIR red, far-red light phototransduction Ref.1 Inferred from mutant phenotype. Source: TAIR response to far red light Inferred from expression pattern. Source: TAIR response to red light Inferred from expression pattern. Source: TAIR
Cellular component	cytoplasm Ref.1 Non-traceable author statement. Source: TAIR plasma membrane Inferred from direct assay. Source: TAIR
Molecular function	protein binding Ref.1 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
At5g64330	Q0WSP1	1	EBI-626200,EBI-1553842
PHOT1	O48963	1	EBI-626200,EBI-1553849
PHYA	P14712	3	EBI-626200,EBI-624446
PHYB	P14713	1	EBI-626200,EBI-300727

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 439	439	Phytochrome kinase substrate 1		PRO_0000058450
Regions
	Compositional bias	31 – 34	4	Poly-Ser
	Compositional bias	365 – 369	5	Poly-Ser
Experimental info
	Sequence conflict	196	1	E → D in AAD38033. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9SWI1-1 [UniParc]. Last modified May 10, 2002. Version 2. Checksum: 7A0A825FF7D3F3FC	FASTA	439	48,085
	10 20 30 40 50 60 MVTLTPSSAS TPKTSFDFMK NNNSHSSLYV SSSSYLSSKE DALVTTKKLM EPSKTLNMSI 70 80 90 100 110 120 NPKQEEFGDE KKMVKKAPED PEIGVFGAEK YFNGDMDSDQ GSSVLSLTNP EVERTVVDSK 130 140 150 160 170 180 QSAKKSTGTP SVRSESSWNS QSVLLQNKLV NSCNSSFKEK KNSNGQIQKV TNNKKSFLAN 190 200 210 220 230 240 LGCKCACSDG DSVDVEEKTS VKRSADPNIS VITMRSSADM NTELIKIQKQ EELSQRKSLE 250 260 270 280 290 300 VFGSPVAIEK KSSVVQKKLP LPPWKSRTEE DDTKSEGSDS SSDLFEIEGL TGNPKPFLTR 310 320 330 340 350 360 QGSDPASPTC YAPSEVSVEW SIVTASAADF SVMSECATSP VRRNRPTQIP RIPITAKSAP 370 380 390 400 410 420 QRRKSSSSSG GNGFLMSCKS HKSVMVSGDL DRRSSMNKTQ PSYVPRFPME TTKPKSFETR 430 RRISNSSISH TQSSLLYSQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"PKS1, a substrate phosphorylated by phytochrome that modulates light signaling in Arabidopsis." Fankhauser C., Yeh K.C., Lagarias J.C., Zhang H., Elich T.D., Chory J. Science 284:1539-1541(1999) [PubMed: 10348744] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF149053 mRNA. Translation: AAD38033.1. AC004138 Genomic DNA. Translation: AAC32913.1. AF325064 mRNA. Translation: AAK17132.1. AY052708 mRNA. Translation: AAK96612.1. AY063721 mRNA. Translation: AAL36071.1.
IPI	IPI00530469.
PIR	E84442. T52304.
RefSeq	NP_565292.1.
UniGene	At.24751
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9SWI1. 4 interactions.
Proteomic databases
PRIDE	Q9SWI1.
Genome annotation databases
GeneID	814823.
GenomeReviews	Gene locus AT2G02950 in contig CT485783_GR.
KEGG	ath:AT2G02950.
NMPDR	fig|3702.1.peg.7889.
Organism-specific databases
TAIR	At2g02950.
Gene expression databases
ArrayExpress	Q9SWI1.
GermOnline	AT2G02950. Arabidopsis thaliana.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

PKS1_ARATH

Accession

Primary (citable) accession number: Q9SWI1
Secondary accession number(s): O80610

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2002
Last sequence update:	May 10, 2002
Last modified:	June 16, 2009
This is version 49 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)