Reviewed, UniProtKB/Swiss-Prot Q9SWH5 (FUT1_ARATH)

Names and origin

Protein names

Recommended name:
    Galactoside 2-alpha-L-fucosyltransferase
    EC=2.4.1.69
Alternative name(s):
    Xyloglucan alpha-(1,2)-fucosyltransferase
      Short name=AtFUT1

Gene names

Name:	FUT1
Synonyms:	FT1
Ordered Locus Names:	At2g03220
ORF Names:	T18E12.11

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	558 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in cell wall biosynthesis. Adds the terminal fucosyl residue on xyloglucan side chains. Ref.1 Ref.4
Catalytic activity	GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein By similarity. Note: Membrane-bound form in trans cisternae of Golgi By similarity.
Tissue specificity	Expressed in roots, stems, leaves, flowers, siliques and seedlings. Ref.4
Sequence similarities	Belongs to the glycosyltransferase 37 family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall biogenesis Inferred from electronic annotation. Source: InterPro cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW xyloglucan biosynthetic process Ref.1 Inferred from direct assay. Source: TAIR
Cellular component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	galactoside 2-alpha-L-fucosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 558	558	Galactoside 2-alpha-L-fucosyltransferase		PRO_0000193909
Regions
	Topological domain	1 – 43	43	Cytoplasmic Potential
	Transmembrane	44 – 64	21	Signal-anchor for type II membrane protein Potential
	Topological domain	65 – 558	494	Lumenal Potential
Amino acid modifications
	Glycosylation	88	1	N-linked (GlcNAc...) Potential
	Glycosylation	504	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	270	1	V → F in AAD41092. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9SWH5-1 [UniParc]. Last modified March 5, 2002. Version 2. Checksum: 1BF0E372231A8ADF	FASTA	558	63,452
	10 20 30 40 50 60 MDQNSYRRRS SPIRTTTGGS KSVNFSELLQ MKYLSSGTMK LTRTFTTCLI VFSVLVAFSM 70 80 90 100 110 120 IFHQHPSDSN RIMGFAEARV LDAGVFPNVT NINSDKLLGG LLASGFDEDS CLSRYQSVHY 130 140 150 160 170 180 RKPSPYKPSS YLISKLRNYE KLHKRCGPGT ESYKKALKQL DQEHIDGDGE CKYVVWISFS 190 200 210 220 230 240 GLGNRILSLA SVFLYALLTD RVLLVDRGKD MDDLFCEPFL GMSWLLPLDF PMTDQFDGLN 250 260 270 280 290 300 QESSRCYGYM VKNQVIDTEG TLSHLYLHLV HDYGDHDKMF FCEGDQTFIG KVPWLIVKTD 310 320 330 340 350 360 NYFVPSLWLI PGFDDELNKL FPQKATVFHH LGRYLFHPTN QVWGLVTRYY EAYLSHADEK 370 380 390 400 410 420 IGIQVRVFDE DPGPFQHVMD QISSCTQKEK LLPEVDTLVE RSRHVNTPKH KAVLVTSLNA 430 440 450 460 470 480 GYAENLKSMY WEYPTSTGEI IGVHQPSQEG YQQTEKKMHN GKALAEMYLL SLTDNLVTSA 490 500 510 520 530 540 WSTFGYVAQG LGGLKPWILY RPENRTTPDP SCGRAMSMEP CFHSPPFYDC KAKTGIDTGT 550 LVPHVRHCED ISWGLKLV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Xyloglucan fucosyltransferase, an enzyme involved in plant cell wall biosynthesis." Perrin R.M., DeRocher A.E., Bar-Peled M., Zeng W., Norambuena L., Orellana A., Raikhel N.V., Keegstra K. Science 284:1976-1979(1999) [PubMed: 10373113] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Characterization of a family of Arabidopsis genes related to xyloglucan fucosyltransferase1." Sarria R., Wagner T.A., O'Neill M.A., Faik A., Wilkerson C.G., Keegstra K., Raikhel N.V. Plant Physiol. 127:1595-1606(2001) [PubMed: 11743104] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF154111 mRNA. Translation: AAD41092.1. AC005313 Genomic DNA. Translation: AAC34480.1. AY139993 mRNA. Translation: AAM98135.1. BT002477 mRNA. Translation: AAO00837.1. BT003401 mRNA. Translation: AAO30064.1.
IPI	IPI00530227.
PIR	T02704.
RefSeq	NP_178421.1.
UniGene	At.20298
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT37. Glycosyltransferase Family 37.
Proteomic databases
PRIDE	Q9SWH5.
Genome annotation databases
GeneID	814851.
GenomeReviews	Gene locus AT2G03220 in contig CT485783_GR.
KEGG	ath:AT2G03220.
NMPDR	fig|3702.1.peg.7919.
Organism-specific databases
TAIR	At2g03220.
Phylogenomic databases
OMA	Q9SWH5. DNYFVPS.
Enzyme and pathway databases
BRENDA	2.4.1.69. 302.
Gene expression databases
ArrayExpress	Q9SWH5.
GermOnline	AT2G03220. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR004938. XG_FTase. [Graphical view]
Pfam	PF03254. XG_FTase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUT1_ARATH

Accession

Primary (citable) accession number: Q9SWH5
Secondary accession number(s): O81052

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	March 5, 2002
Last modified:	June 16, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)