Reviewed, UniProtKB/Swiss-Prot Q9SWG0 (IVD_ARATH)

Names and origin

Protein names

Recommended name:
    Isovaleryl-CoA dehydrogenase, mitochondrial
      Short name=IVD
    EC=1.3.99.10

Gene names

Name:	IVD
Ordered Locus Names:	At3g45300
ORF Names:	F18N11.6

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	409 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	3-methylbutanoyl-CoA + acceptor = 3-methylbut-2-enoyl-CoA + reduced acceptor.
Cofactor	FAD.
Pathway	Amino-acid degradation; L-leucine degradation; HMG-CoA from 3-isovaleryl-CoA: step 1/3.
Subunit structure	Homodimer By similarity.
Subcellular location	Mitochondrion.
Sequence similarities	Belongs to the acyl-CoA dehydrogenase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	isovaleryl-CoA dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 25	25	Mitochondrion By similarity
	Chain	26 – 409	384	Isovaleryl-CoA dehydrogenase, mitochondrial		PRO_0000000535
Sites
	Active site	270	1	Proton acceptor By similarity
Experimental info
	Sequence conflict	49	1	N → I Ref.1 Ref.5
	Sequence conflict	282	1	M → V in AAL32645 and AAM91199. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q9SWG0-1 [UniParc]. Last modified May 30, 2006. Version 2. Checksum: 6D3D86C8F632067C	FASTA	409	44,773
	10 20 30 40 50 60 MQRFFSARSI LGYAVKTRRR SFSSRSSSLL FDDTQLQFKE SVSKFAQDNI APHAERIDKT 70 80 90 100 110 120 NSFPKDVNLW KLMGEFNLHG ITAPEEYGGL GLGYLYHCIA MEEISRASGS VALSYGAHSN 130 140 150 160 170 180 LCINQLVRNG TAAQKEKYLP KLISGEHVGA LAMSEPNAGS DVVGMKCKAE KVDGGYILNG 190 200 210 220 230 240 NKMWCTNGPS AETLVVYAKT DTKAGSKGIT AFIIEKGMTG FSTAQKLDKL GMRGSDTCEL 250 260 270 280 290 300 VFENCFVPEE NILDKEGKGV YVLMSGLDLE RLVLAAGPLG IMQACLDNVL PYIRQREQFG 310 320 330 340 350 360 RPVGEFQFIQ GKVADMYTAL QSSRSYVYSV ARDCDNGKVD PKDCAGTILC AAERATQVAL 370 380 390 400 QAIQCLGGNG YINEYATGRL LRDAKLYEIG AGTSEIRRIV IGRELFKEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"In plants a putative isovaleryl-CoA-dehydrogenase is located in mitochondria." Daeschner K., Thalheim C., Guha C., Brennicke A., Binder S. Plant Mol. Biol. 39:1275-1282(1999) [PubMed: 10380813] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
[2]	"Purification, characterization and cloning of isovaleryl-CoA dehydrogenase from higher plant mitochondria." Faivre-Nitschke S.E., Couee I., Vermel M., Grienenberger J.-M., Gualberto J.M. Eur. J. Biochem. 268:1332-1339(2001) [PubMed: 11231285] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT. Strain: cv. Columbia.
[3]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins." Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H. Plant Cell 16:241-256(2004) [PubMed: 14671022] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF160729 mRNA. Translation: AAD45605.1. Y12695 mRNA. Translation: CAA73227.1. AL132953 Genomic DNA. Translation: CAB72479.1. AY062567 mRNA. Translation: AAL32645.1. AY128799 mRNA. Translation: AAM91199.1. AY087286 mRNA. Translation: AAM64839.1.
PIR	T47470.
RefSeq	NP_190116.1.
UniGene	At.428
3D structure databases
HSSP	HSSP built from PDB template 1IVH based on UniProtKB P26440.
ModBase	Search...
Genome annotation databases
GeneID	823668.
GenomeReviews	Gene locus AT3G45300 in contig BA000014_GR.
KEGG	ath:AT3G45300.
NMPDR	fig|3702.1.peg.15742.
Organism-specific databases
GeneFarm	4384.
TAIR	At3g45300.
Gene expression databases
ArrayExpress	Q9SWG0.
GermOnline	AT3G45300. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR006091. Acyl-CoA_DHase/Oxase_M. IPR006089. Acyl-CoA_DHase_CS. IPR006092. Acyl-CoA_DHase_N. IPR006090. Acyl-CoA_Oxase/DHase_1. IPR013786. AcylCoA_DH/ox_N. IPR013764. AcylCoA_oxidase/DH_1/2_C. [Graphical view]
Gene3D	G3DSA:2.40.110.10. Acyl_CoA_DH/ox_M. 1 hit. G3DSA:1.10.540.10. AcylCoA_DH/ox_N. 1 hit. G3DSA:1.20.140.10. AcylCoA_DH_1/2_C. 1 hit.
Pfam	PF00441. Acyl-CoA_dh_1. 1 hit. PF02770. Acyl-CoA_dh_M. 1 hit. PF02771. Acyl-CoA_dh_N. 1 hit. [Graphical view]
PROSITE	PS00072. ACYL_COA_DH_1. 1 hit. PS00073. ACYL_COA_DH_2. 1 hit. [Graphical view]
BLOCKS	Search...
ProtoNet	Search...

Entry information

Entry name

IVD_ARATH

Accession

Primary (citable) accession number: Q9SWG0
Secondary accession number(s): Q8W4G7, Q9XFT2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	May 30, 2006
Last modified:	November 4, 2008
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)