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Q9SWE5 (HAL3A_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphopantothenoylcysteine decarboxylase

Short name=PPCDC
EC=4.1.1.36
Alternative name(s):
Halotolerance protein Hal3a
Short name=AtCoaC
Short name=AtHal3a
Gene names
Name:HAL3A
Ordered Locus Names:At3g18030
ORF Names:MBG14.2
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length209 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in plant growth and salt and osmotic tolerance. Catalyzes the decarboxylation of 4'-phosphopantothenoylcysteine to 4'-phosphopantetheine, a key step in coenzyme A biosynthesis. The enzyme is also able to decarboxylate pantothenoylcysteine to pantothenoylcysteamine.

Catalytic activity

N-((R)-4'-phosphopantothenoyl)-L-cysteine = pantotheine 4'-phosphate + CO2.

Cofactor

Binds 1 FMN per subunit.

Pathway

Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 3/5.

Subunit structure

Homotrimer.

Tissue specificity

Expressed in roots, shoots, leaves, flowers, developing siliques and seeds with highest expression in seed embryos and phloem. Ref.1

Induction

By salt stress. Ref.1

Sequence similarities

Belongs to the HFCD (homooligomeric flavin containing Cys decarboxylase) superfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 209209Phosphopantothenoylcysteine decarboxylase
PRO_0000182032

Regions

Nucleotide binding106 – 1094FMN

Sites

Active site1751Proton donor
Binding site591FMN
Binding site1421Substrate

Experimental info

Mutagenesis301V → I: No effect on activity. Ref.7
Mutagenesis331I → L or V: No effect on activity. Ref.7
Mutagenesis341K → N or R: No effect on activity. Ref.7
Mutagenesis341K → Q: Small decrease of activity. Ref.7
Mutagenesis901H → N: Complete loss of activity. Ref.6
Mutagenesis951R → Q: Very low activity. Can reduce the oxidied intermediate. Ref.7
Mutagenesis1421N → D: Complete loss of activity. Ref.7
Mutagenesis1451M → L: Complete loss of activity. Ref.7
Mutagenesis1741A → S: Significantly reduced activity. Can reduce the oxidied intermediate. Ref.7
Mutagenesis1741A → V: No effect. Ref.7
Mutagenesis1751C → S: Complete loss of activity. Ref.7
Mutagenesis1771D → N: Very low activity. Can reduce the oxidied intermediate. Ref.7
Mutagenesis1791G → A: Very low activity. Can reduce the oxidied intermediate. Ref.7
Mutagenesis1811G → A: Significantly reduced activity. Ref.7

Secondary structure

.................................... 209
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9SWE5 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 3AB1BB364F8E40DE

FASTA20923,355
        10         20         30         40         50         60 
MENGKRDRQD MEVNTTPRKP RVLLAASGSV AAIKFGNLCH CFTEWAEVRA VVTKSSLHFL 

        70         80         90        100        110        120 
DKLSLPQEVT LYTDEDEWSS WNKIGDPVLH IELRRWADVL VIAPLSANTL GKIAGGLCDN 

       130        140        150        160        170        180 
LLTCIIRAWD YTKPLFVAPA MNTLMWNNPF TERHLLSLDE LGITLIPPIK KRLACGDYGN 

       190        200 
GAMAEPSLIY STVRLFWESQ AHQQTGGTS 

« Hide

References

« Hide 'large scale' references
[1]"Arabidopsis thaliana AtHAL3: a flavoprotein related to salt and osmotic tolerance and plant growth."
Espinosa-Ruiz A., Belles J.M., Serrano R., Culianez-Macia F.A.
Plant J. 20:529-539(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, INDUCTION.
Strain: cv. Columbia.
[2]"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Arabidopsis ORF clones."
Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R.
Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Arabidopsis thaliana flavoprotein AtHAL3a catalyzes the decarboxylation of 4'-Phosphopantothenoylcysteine to 4'-phosphopantetheine, a key step in coenzyme A biosynthesis."
Kupke T., Hernandez-Acosta P., Steinbacher S., Culianez-Macia F.A.
J. Biol. Chem. 276:19190-19196(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION OF ACTIVITY, MUTAGENESIS OF HIS-90.
[7]"Molecular characterization of the Arabidopsis thaliana flavoprotein AtHAL3a reveals the general reaction mechanism of 4'-phosphopantothenoylcysteine decarboxylases."
Hernandez-Acosta P., Schmid D.G., Jung G., Culianez-Macia F.A., Kupke T.
J. Biol. Chem. 277:20490-20498(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION OF ACTIVITY, MUTAGENESIS OF VAL-30; ILE-33; LYS-34; ARG-95; ASN-142; MET-145; ALA-174; CYS-175; ASP-177; GLY-179 AND GLY-181.
[8]"The X-ray structure of the FMN-binding protein AtHal3 provides the structural basis for the activity of a regulatory subunit involved in signal transduction."
Albert A., Martinez-Ripoll M., Espinosa-Ruiz A., Yenush L., Culianez-Macia F.A., Serrano R.
Structure 8:961-969(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEX WITH FMN.
[9]"Crystal structure of the plant PPC decarboxylase AtHAL3a complexed with an ene-thiol reaction intermediate."
Steinbacher S., Hernandez-Acosta P., Bieseler B., Blaesse M., Huber R., Culianez-Macia F.A., Kupke T.
J. Mol. Biol. 327:193-202(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.21 ANGSTROMS) OF MUTANT SER-175 IN COMPLEX WITH SUBSTRATE AND FMN.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF166262 Genomic DNA. Translation: AAD51616.1.
AB026641 Genomic DNA. Translation: BAB01331.1.
CP002686 Genomic DNA. Translation: AEE76036.1.
BT014781 mRNA. Translation: AAT41764.1.
BT015689 mRNA. Translation: AAU29466.1.
AK228559 mRNA. Translation: BAF00478.1.
RefSeqNP_188430.1. NM_112684.4.
UniGeneAt.24652.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1E20X-ray2.02A1-209[»]
1MVLX-ray2.00A1-209[»]
1MVNX-ray2.21A1-209[»]
ProteinModelPortalQ9SWE5.
SMRQ9SWE5. Positions 18-202.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid6660. 1 interaction.

Proteomic databases

PaxDbQ9SWE5.
PRIDEQ9SWE5.

Protocols and materials databases

DNASU821327.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G18030.1; AT3G18030.1; AT3G18030.
GeneID821327.
KEGGath:AT3G18030.

Organism-specific databases

TAIRAT3G18030.

Phylogenomic databases

eggNOGCOG0452.
HOGENOMHOG000187629.
InParanoidQ9SWE5.
KOK01598.
OMAMEPKASC.
PhylomeDBQ9SWE5.
ProtClustDBPLN02496.

Enzyme and pathway databases

UniPathwayUPA00241; UER00354.

Gene expression databases

GenevestigatorQ9SWE5.

Family and domain databases

Gene3D3.40.50.1950. 1 hit.
InterProIPR003382. Flavoprotein.
[Graphical view]
PfamPF02441. Flavoprotein. 1 hit.
[Graphical view]
SUPFAMSSF52507. SSF52507. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ9SWE5.

Entry information

Entry nameHAL3A_ARATH
AccessionPrimary (citable) accession number: Q9SWE5
Secondary accession number(s): Q6ID92
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 1, 2000
Last modified: February 19, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names