Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9SWE5

- HAL3A_ARATH

UniProt

Q9SWE5 - HAL3A_ARATH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Phosphopantothenoylcysteine decarboxylase

Gene

HAL3A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in plant growth and salt and osmotic tolerance. Catalyzes the decarboxylation of 4'-phosphopantothenoylcysteine to 4'-phosphopantetheine, a key step in coenzyme A biosynthesis. The enzyme is also able to decarboxylate pantothenoylcysteine to pantothenoylcysteamine.2 Publications

Catalytic activityi

N-((R)-4'-phosphopantothenoyl)-L-cysteine = pantotheine 4'-phosphate + CO2.

Cofactori

FMNNote: Binds 1 FMN per subunit.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei140 – 1401FMN; via carbonyl oxygen2 Publications
Binding sitei142 – 1421Substrate; via amide nitrogen
Active sitei175 – 1751Proton donor
Binding sitei183 – 1831Substrate; via amide nitrogen

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi28 – 303FMN2 Publications
Nucleotide bindingi53 – 553FMN2 Publications
Nucleotide bindingi106 – 1094FMN2 Publications

GO - Molecular functioni

  1. phosphopantothenoylcysteine decarboxylase activity Source: TAIR

GO - Biological processi

  1. coenzyme A biosynthetic process Source: TAIR
  2. hyperosmotic salinity response Source: TAIR
  3. regulation of growth Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Coenzyme A biosynthesis, Growth regulation, Stress response

Keywords - Ligandi

Flavoprotein, FMN

Enzyme and pathway databases

ReactomeiREACT_248597. Coenzyme A biosynthesis.
UniPathwayiUPA00241; UER00354.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphopantothenoylcysteine decarboxylase (EC:4.1.1.36)
Short name:
PPCDC
Alternative name(s):
AtCoaC1
Halotolerance protein Hal3a
Short name:
AtHal3a
Gene namesi
Name:HAL3A
Synonyms:COAC1
Ordered Locus Names:At3g18030
ORF Names:MBG14.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G18030.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: TAIR
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but homozygous double mutants hal3a-1 and hal3b are embryonic lethal.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi30 – 301V → I: No effect on activity. 1 Publication
Mutagenesisi33 – 331I → L or V: No effect on activity. 1 Publication
Mutagenesisi34 – 341K → N or R: No effect on activity. 1 Publication
Mutagenesisi34 – 341K → Q: Small decrease of activity. 1 Publication
Mutagenesisi90 – 901H → N: Complete loss of activity. 1 Publication
Mutagenesisi95 – 951R → Q: Very low activity. Can reduce the oxidied intermediate. 1 Publication
Mutagenesisi142 – 1421N → D: Complete loss of activity. 1 Publication
Mutagenesisi145 – 1451M → L: Complete loss of activity. 1 Publication
Mutagenesisi174 – 1741A → S: Significantly reduced activity. Can reduce the oxidied intermediate. 1 Publication
Mutagenesisi174 – 1741A → V: No effect. 1 Publication
Mutagenesisi175 – 1751C → S: Complete loss of activity. 1 Publication
Mutagenesisi177 – 1771D → N: Very low activity. Can reduce the oxidied intermediate. 1 Publication
Mutagenesisi179 – 1791G → A: Very low activity. Can reduce the oxidied intermediate. 1 Publication
Mutagenesisi181 – 1811G → A: Significantly reduced activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 209209Phosphopantothenoylcysteine decarboxylasePRO_0000182032Add
BLAST

Proteomic databases

PaxDbiQ9SWE5.
PRIDEiQ9SWE5.

Expressioni

Tissue specificityi

Expressed in roots, shoots, leaves, flowers, developing siliques and seeds with highest expression in seed embryos and phloem.1 Publication

Inductioni

By salt stress.1 Publication

Gene expression databases

GenevestigatoriQ9SWE5.

Interactioni

Subunit structurei

Homotrimer.2 Publications

Protein-protein interaction databases

BioGridi6660. 1 interaction.

Structurei

Secondary structure

1
209
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi21 – 266Combined sources
Helixi30 – 345Combined sources
Helixi35 – 439Combined sources
Beta strandi46 – 527Combined sources
Helixi55 – 584Combined sources
Helixi62 – 643Combined sources
Beta strandi70 – 723Combined sources
Helixi76 – 805Combined sources
Helixi89 – 968Combined sources
Beta strandi98 – 1058Combined sources
Helixi107 – 1159Combined sources
Helixi121 – 1277Combined sources
Beta strandi135 – 1395Combined sources
Helixi143 – 1475Combined sources
Helixi149 – 16113Combined sources
Beta strandi169 – 1724Combined sources
Beta strandi178 – 1825Combined sources
Helixi186 – 19914Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1E20X-ray2.02A1-209[»]
1MVLX-ray2.00A1-209[»]
1MVNX-ray2.21A1-209[»]
ProteinModelPortaliQ9SWE5.
SMRiQ9SWE5. Positions 18-202.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9SWE5.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni172 – 1743Substrate binding

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0452.
HOGENOMiHOG000187629.
KOiK01598.
OMAiKHFYNPQ.
PhylomeDBiQ9SWE5.

Family and domain databases

Gene3Di3.40.50.1950. 1 hit.
InterProiIPR003382. Flavoprotein.
[Graphical view]
PfamiPF02441. Flavoprotein. 1 hit.
[Graphical view]
SUPFAMiSSF52507. SSF52507. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9SWE5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MENGKRDRQD MEVNTTPRKP RVLLAASGSV AAIKFGNLCH CFTEWAEVRA
60 70 80 90 100
VVTKSSLHFL DKLSLPQEVT LYTDEDEWSS WNKIGDPVLH IELRRWADVL
110 120 130 140 150
VIAPLSANTL GKIAGGLCDN LLTCIIRAWD YTKPLFVAPA MNTLMWNNPF
160 170 180 190 200
TERHLLSLDE LGITLIPPIK KRLACGDYGN GAMAEPSLIY STVRLFWESQ

AHQQTGGTS
Length:209
Mass (Da):23,355
Last modified:May 1, 2000 - v1
Checksum:i3AB1BB364F8E40DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF166262 Genomic DNA. Translation: AAD51616.1.
AB026641 Genomic DNA. Translation: BAB01331.1.
CP002686 Genomic DNA. Translation: AEE76036.1.
BT014781 mRNA. Translation: AAT41764.1.
BT015689 mRNA. Translation: AAU29466.1.
AK228559 mRNA. Translation: BAF00478.1.
RefSeqiNP_188430.1. NM_112684.4.
UniGeneiAt.24652.

Genome annotation databases

EnsemblPlantsiAT3G18030.1; AT3G18030.1; AT3G18030.
GeneIDi821327.
KEGGiath:AT3G18030.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF166262 Genomic DNA. Translation: AAD51616.1 .
AB026641 Genomic DNA. Translation: BAB01331.1 .
CP002686 Genomic DNA. Translation: AEE76036.1 .
BT014781 mRNA. Translation: AAT41764.1 .
BT015689 mRNA. Translation: AAU29466.1 .
AK228559 mRNA. Translation: BAF00478.1 .
RefSeqi NP_188430.1. NM_112684.4.
UniGenei At.24652.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1E20 X-ray 2.02 A 1-209 [» ]
1MVL X-ray 2.00 A 1-209 [» ]
1MVN X-ray 2.21 A 1-209 [» ]
ProteinModelPortali Q9SWE5.
SMRi Q9SWE5. Positions 18-202.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 6660. 1 interaction.

Proteomic databases

PaxDbi Q9SWE5.
PRIDEi Q9SWE5.

Protocols and materials databases

DNASUi 821327.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G18030.1 ; AT3G18030.1 ; AT3G18030 .
GeneIDi 821327.
KEGGi ath:AT3G18030.

Organism-specific databases

TAIRi AT3G18030.

Phylogenomic databases

eggNOGi COG0452.
HOGENOMi HOG000187629.
KOi K01598.
OMAi KHFYNPQ.
PhylomeDBi Q9SWE5.

Enzyme and pathway databases

UniPathwayi UPA00241 ; UER00354 .
Reactomei REACT_248597. Coenzyme A biosynthesis.

Miscellaneous databases

EvolutionaryTracei Q9SWE5.

Gene expression databases

Genevestigatori Q9SWE5.

Family and domain databases

Gene3Di 3.40.50.1950. 1 hit.
InterProi IPR003382. Flavoprotein.
[Graphical view ]
Pfami PF02441. Flavoprotein. 1 hit.
[Graphical view ]
SUPFAMi SSF52507. SSF52507. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Arabidopsis thaliana AtHAL3: a flavoprotein related to salt and osmotic tolerance and plant growth."
    Espinosa-Ruiz A., Belles J.M., Serrano R., Culianez-Macia F.A.
    Plant J. 20:529-539(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, INDUCTION.
    Strain: cv. Columbia.
  2. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
    DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Arabidopsis ORF clones."
    Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R.
    Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Arabidopsis thaliana flavoprotein AtHAL3a catalyzes the decarboxylation of 4'-Phosphopantothenoylcysteine to 4'-phosphopantetheine, a key step in coenzyme A biosynthesis."
    Kupke T., Hernandez-Acosta P., Steinbacher S., Culianez-Macia F.A.
    J. Biol. Chem. 276:19190-19196(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION OF ACTIVITY, MUTAGENESIS OF HIS-90.
  7. "Molecular characterization of the Arabidopsis thaliana flavoprotein AtHAL3a reveals the general reaction mechanism of 4'-phosphopantothenoylcysteine decarboxylases."
    Hernandez-Acosta P., Schmid D.G., Jung G., Culianez-Macia F.A., Kupke T.
    J. Biol. Chem. 277:20490-20498(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION OF ACTIVITY, MUTAGENESIS OF VAL-30; ILE-33; LYS-34; ARG-95; ASN-142; MET-145; ALA-174; CYS-175; ASP-177; GLY-179 AND GLY-181.
  8. "4'-phosphopantetheine and coenzyme A biosynthesis in plants."
    Kupke T., Hernandez-Acosta P., Culianez-Macia F.A.
    J. Biol. Chem. 278:38229-38237(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: cv. Columbia.
  9. "An Arabidopsis mutant impaired in coenzyme A biosynthesis is sugar dependent for seedling establishment."
    Rubio S., Larson T.R., Gonzalez-Guzman M., Alejandro S., Graham I.A., Serrano R., Rodriguez P.L.
    Plant Physiol. 140:830-843(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  10. "The X-ray structure of the FMN-binding protein AtHal3 provides the structural basis for the activity of a regulatory subunit involved in signal transduction."
    Albert A., Martinez-Ripoll M., Espinosa-Ruiz A., Yenush L., Culianez-Macia F.A., Serrano R.
    Structure 8:961-969(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEX WITH FMN.
  11. "Crystal structure of the plant PPC decarboxylase AtHAL3a complexed with an ene-thiol reaction intermediate."
    Steinbacher S., Hernandez-Acosta P., Bieseler B., Blaesse M., Huber R., Culianez-Macia F.A., Kupke T.
    J. Mol. Biol. 327:193-202(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.21 ANGSTROMS) OF MUTANT SER-175 IN COMPLEX WITH SUBSTRATE ANALOG AND FMN.

Entry informationi

Entry nameiHAL3A_ARATH
AccessioniPrimary (citable) accession number: Q9SWE5
Secondary accession number(s): Q6ID92
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 1, 2000
Last modified: November 26, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3