Reviewed, UniProtKB/Swiss-Prot Q9SWB4 (PARP3_SOYBN)

Names and origin

Protein names

Recommended name:
    Poly [ADP-ribose] polymerase 3
      Short name=PARP-3
    EC=2.4.2.30
Alternative name(s):
    ADPRT 3
    NAD(+) ADP-ribosyltransferase 3
    Poly[ADP-ribose] synthetase 3

Gene names

Name:	PARP3
Synonyms:	PM38

Organism

Glycine max (Soybean)

Taxonomic identifier

3847 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Glycine

Protein attributes

Sequence length	815 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks By similarity.
Catalytic activity	NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.
Subcellular location	Nucleus Potential.
Sequence similarities	Contains 1 BRCT domain. Contains 1 PARP alpha-helical domain. Contains 1 PARP catalytic domain. Contains 1 SAP domain.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	DNA-binding NAD
Molecular function	Glycosyltransferase Transferase
PTM	ADP-ribosylation
Gene Ontology (GO)
Biological process	protein amino acid ADP-ribosylation Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ ADP-ribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 815	815	Poly [ADP-ribose] polymerase 3		PRO_0000260506
Regions
	Domain	69 – 103	35	SAP
	Domain	182 – 274	93	BRCT
	Domain	449 – 568	120	PARP alpha-helical
	Domain	577 – 808	232	PARP catalytic

Sequences

	Sequence		Length	Mass (Da)
	Q9SWB4-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 2795159D82B70D12	FASTA	815	91,689
	10 20 30 40 50 60 MKVQETRSHV HALGEEEKVM TRKQKAESKA HEVEHSPKKA KVEKEDGHIN GKSETGVAEE 70 80 90 100 110 120 YDEFCKATTE HLPLEQMRDI LEANGLDSSG SDLEITRRCQ DLLFYGALDK CSVCNGSLEF 130 140 150 160 170 180 DGRRYVCRGF YSEWASCTFS TRNPPRKQEP IKLPDSVQNS LASDLLKKYQ DPSHRPHRDL 190 200 210 220 230 240 GLAEKPFTGM MISLMGRLTR THHYWKTTIE KHGGKVANSI IGSTCLVASP AERERGGTSK 250 260 270 280 290 300 LAEAMERSIP VVREAWLIDS IEKQEPQPLE AYDLVSDLSV DGKGIPWDKQ DPGEEAIESL 310 320 330 340 350 360 SAELKLYGKR GVYKDTKLQE QGGKIFERDG ILYNCAFSVC DQGRGLNDYC VMQLIVVPEN 370 380 390 400 410 420 RLHLYFKKGR VGDDPNAEER LEEWDNVDGA LKEFVRLFEE ITGNEFEPWE REKKFQKKPL 430 440 450 460 470 480 KFYPIDMDDG IEVRHGALGL RQLGIAATHC KLEPLVANFM KVLCSQEIYK YALMEMGYDC 490 500 510 520 530 540 PDLPIGMVTN LHLKKCEDVL LEFIDKVKSL KETGPKAEAV WTDFSQRWFT LMHSTRPFNF 550 560 570 580 590 600 RDYQEIADHA AAALEGVRDI TQASHLIGDM TGSTIDDPLS ETYKKLGCSI SALDKSSDDY 610 620 630 640 650 660 EMIVKYLEKT YEPVKVGDIE YGVSVENIFA VQTGGCPSYE DIIKLPNKVL LWCGSRSSNL 670 680 690 700 710 720 LRHLQKGFLP AICSLPIPGY MFGKAIVCSD AAAEAARYGF TAVDRPEGFL VLAIASLGNE 730 740 750 760 770 780 ITELKTPPED ASSLEEKKVG VKGPGKKKTD ESEHFVWKDD IKVPCGKLVA SDHQDSPLEY 790 800 810 NEYAVYDKKR ARISYLVGVK YEEKEEKGAV IDTAE

References

[1]	"Characterization of a soybean seed maturation protein, PM38." Chow T.Y., Lin T.Y., Lin T.Y., Liu S.M., Hsing Y.I.C. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Shi-shi.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF169023 mRNA. Translation: AAD51626.1.
UniGene	Gma.8042
3D structure databases
HSSP	HSSP built from PDB template 1GS0 based on UniProtKB O88554.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.4.2.30. 299.
Gene expression databases
Genevestigator	Q9SWB4.
Family and domain databases
InterPro	IPR001357. BRCT. IPR012982. PADR1. IPR012317. PARP_catalytic. IPR004102. PARP_reg. IPR003034. SAP_DNA_bd. IPR008893. WGR. [Graphical view]
Gene3D	G3DSA:1.20.142.10. PARP_reg. 1 hit.
Pfam	PF00533. BRCT. 1 hit. PF08063. PADR1. 1 hit. PF00644. PARP. 1 hit. PF05406. WGR. 1 hit. [Graphical view]
SMART	SM00292. BRCT. 1 hit. SM00773. WGR. 1 hit. [Graphical view]
PROSITE	PS50172. BRCT. 1 hit. PS51060. PARP_ALPHA_HD. 1 hit. PS51059. PARP_CATALYTIC. 1 hit. PS50800. SAP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PARP3_SOYBN

Accession

Primary (citable) accession number: Q9SWB4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	May 1, 2000
Last modified:	October 13, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)