Q9SW96 (SYNC1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Asparagine--tRNA ligase, cytoplasmic 1
EC=6.1.1.22

Alternative name(s):
Asparaginyl-tRNA synthetase 1
Short name=AsnRS 1
Protein EMBRYO DEFECTIVE 2755

Gene names

Name:	SYNC1
Synonyms:	EMB2755
Ordered Locus Names:	At5g56680
ORF Names:	MIK19.13

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	572 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Potentially protective antigen in lymphatic filariasis.
Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn).
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Contains 1 WHEP-TRS domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro embryo development ending in seed dormancy Inferred from mutant phenotype. Source: TAIR response to cadmium ion Inferred from expression pattern. Source: TAIR
Cellular component	chloroplast Inferred from direct assay Ref.1. Source: TAIR cytosol Inferred from direct assay. Source: TAIR mitochondrion Inferred from direct assay Ref.1. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW asparagine-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 572	572	Asparagine--tRNA ligase, cytoplasmic 1		PRO_0000176491
Regions
	Domain	236 – 292	57	WHEP-TRS

Sequences

	Sequence		Length	Mass (Da)
	Q9SW96 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: DBFBCF0139A48770	FASTA	572	63,783
	10 20 30 40 50 60 MADEIVPPAT QLAAVSLEND GSTVQRAQFS NRVLIRTILD RPDGGAGLAG QTVRIGGWVK 70 80 90 100 110 120 SGRDQGKRTF SFLAVNDGSC PANLQVMVDP SLYDVSNLVA TGTCVTVDGV LKVPPKGKGT 130 140 150 160 170 180 QQQIELNVVK VIDVGTVDAS KYPLPKTKLT LETLRDVLHL RSRTNSISAV ARIRNALAFA 190 200 210 220 230 240 THSFFQEHSF LYIHTPIITT SDCEGAGEMF QATTLINYTE RLEQDLIDNP PPTEADVEAA 250 260 270 280 290 300 RLIVIERGNV VAELKAAKAS KEAITAAVAE LKIAKETFAH IDERSRLRPG LPKKDGNIDY 310 320 330 340 350 360 SKDFFGRQAF LTVSGQLQVE TYACALSNVY TFGPTFRAEN SHTSRHLAEF WMVEPEIAFA 370 380 390 400 410 420 DLEDDMNCAE AYVKYMCNWL LEKCYADMEL MAKNFDSGCI DRLKLVASTP FGRITYTKAI 430 440 450 460 470 480 ELLEEAVAKG KEFDNNVEWG IDLASEHERY LTEVLFQKPL IVYNYPKGIK AFYMRLNDDE 490 500 510 520 530 540 KTVAAMDVLV PKVGELIGGS QREERYDVIK KRIEEMGLPI EPYEWYLDLR RYGTVKHCGF 550 560 570 GLGFERMILF ATGLDNIRDV IPFPRYPGKA DL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Duplication and quadruplication of Arabidopsis thaliana cysteinyl- and asparaginyl-tRNA synthetase genes of organellar origin." Peeters N.M., Chapron A., Giritch A., Grandjean O., Lancelin D., Lhomme T., Vivrel A., Small I. J. Mol. Evol. 50:413-423(2000) [PubMed: 10824085] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones." Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S. DNA Res. 5:203-216(1998) [PubMed: 9734815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF170909 mRNA. Translation: AAD46681.1. AB013392 Genomic DNA. Translation: BAB09886.1. CP002688 Genomic DNA. Translation: AED96795.1.
IPI	IPI00548665.
RefSeq	NP_200479.1. NM_125051.3.
UniGene	At.22552.
3D structure databases
ProteinModelPortal	Q9SW96.
SMR	Q9SW96. Positions 44-571.
ModBase	Search...
Proteomic databases
PRIDE	Q9SW96.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G56680.1; AT5G56680.1; AT5G56680.
GeneID	835769.
GenomeReviews	Gene locus AT5G56680 in contig BA000015_GR.
KEGG	ath:AT5G56680.
NMPDR	fig|3702.1.peg.27608.
Organism-specific databases
GeneFarm	2800. 269.
TAIR	At5g56680.
Phylogenomic databases
GeneTree	EPGT00070000029076.
HOGENOM	HBG745843.
InParanoid	Q9SW96.
OMA	AVDYPIT.
PhylomeDB	Q9SW96.
ProtClustDB	PLN02221.
Gene expression databases
ArrayExpress	Q9SW96.
Genevestigator	Q9SW96.
GermOnline	AT5G56680. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. IPR009068. S15_NS1_RNA-bd. IPR000738. WHEP-TRS. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:1.10.287.10. S15_NS1_RNA_bd. 1 hit.
KO	K01893.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF6. PTHR22594:SF6. 1 hit.
Pfam	PF00152. tRNA-synt_2. 2 hits. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SMART	SM00991. WHEP-TRS. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00457. AsnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. PS00762. WHEP_TRS_1. False negative. PS51185. WHEP_TRS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYNC1_ARATH

Accession

Primary (citable) accession number: Q9SW96

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 31, 2002
Last sequence update:	May 1, 2000
Last modified:	January 25, 2012
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program