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Reviewed, UniProtKB/Swiss-Prot Q9SW95 (SYNC2_ARATH)

Last modified November 3, 2009. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Asparaginyl-tRNA synthetase, cytoplasmic 2
    EC=6.1.1.22
Alternative name(s):
    Asparagine--tRNA ligase 2
      Short name=AsnRS 2
Gene names
Name: SYNC2
Synonyms: NS2
Ordered Locus Names: At3g07420
ORF Names: F21O3.13
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length638 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Catalytic activity

ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn).

Subcellular location

Cytoplasm Probable.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.

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Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processtRNA aminoacylation for protein translation

Inferred from electronic annotation. Source: InterPro

   Cellular componentchloroplast Ref.1

Inferred from direct assay. Source: TAIR

mitochondrion Ref.1

Inferred from direct assay. Source: TAIR

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

asparagine-tRNA ligase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 638638Asparaginyl-tRNA synthetase, cytoplasmic 2
PRO_0000176492

Experimental info

Sequence conflict241S → L in AAD46682. Ref.1
Sequence conflict2841L → F in AAD46682. Ref.1
Sequence conflict3291R → Q in AAD46682. Ref.1
Sequence conflict4601S → L Ref.3
Sequence conflict4601S → L Ref.4

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Sequences

Sequence LengthMass (Da)Tools
Q9SW95-1 [UniParc].

Last modified January 31, 2002. Version 2.
Checksum: C90838CF5649FF8C

FASTA63871,364
        10         20         30         40         50         60 
MESHGKTHQK EHDNDLSPKP ITLSKYSKRV ELKTLLDRSD RGAGLAGKRV VIGGWVKSAR 

        70         80         90        100        110        120 
AVKKNSPPPP LPVVAAPSPS SGGDQAHTTA NIRCTEIIQS KMNIFKRFFD VLSGGGKTYP 

       130        140        150        160        170        180 
IFDKTELAGQ KAVPPPEYVF YFLISDGSSI SSLQVVVDSA LSTVPATQLM ALGTCIVAEG 

       190        200        210        220        230        240 
VLRLPLAASA KHVIELEAEK LLHVGTVDPE KYPLSKKQLP LHMLRDFSHF RPRTTTVGSV 

       250        260        270        280        290        300 
TRVHSALTLA SHTFLQYHGF QYVQVPVITT TTGFGEMFRV TTLLGKTDDK EEKKPPVQEK 

       310        320        330        340        350        360 
DGFSIDTVKA VIKEKTRLID HLKRSDSNRE TVVAAVHDLK KTNDLASQIE MKQKSKTGTL 

       370        380        390        400        410        420 
VKPEKLDFSK DFFGRDTYLT ASGRFHLESY ASALGKVYTF GPRFIADKID NARHLAEKWN 

       430        440        450        460        470        480 
VETEMAFAEL DDAMDCADEY FKFLCKYVLE NRDEDMKFIS KRVDKTITTR LEATASSSLL 

       490        500        510        520        530        540 
RFSYTEVISL LQKATTTKFE TKPEWGVALT TEHLSYLTDE IYKGPVIVHT YPKAIKQFYV 

       550        560        570        580        590        600 
RLNDDKKTVA AFDLVVPKVG VVITGSQNEE RFEILDARIG ESGFTREKFE WYLDLRRHGT 

       610        620        630 
VKHSGISLSM EQMLLYATGL PDIKDAIPFP RSWGKANN

« Hide

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References

« Hide 'large scale' references
[1]"Duplication and quadruplication of Arabidopsis thaliana cysteinyl- and asparaginyl-tRNA synthetase genes of organellar origin."
Peeters N.M., Chapron A., Giritch A., Grandjean O., Lancelin D., Lhomme T., Vivrel A., Small I.
J. Mol. Evol. 50:413-423(2000) [PubMed: 10824085] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed: 11130713] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]"Functional annotation of a full-length Arabidopsis cDNA collection."
Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K.
Science 296:141-145(2002) [PubMed: 11910074] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Structure and expression of an asparaginyl-tRNA synthetase gene located on chromosome IV of Arabidopsis thaliana and adjacent to a novel large gene of 15 exons."
Aubourg S., Cheron A., Kreis M., Lecharny A.
Biochim. Biophys. Acta 1398:225-231(1998) [PubMed: 9655910] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 363-638.
Strain: cv. Columbia.
Tissue: Leaf.
+Additional computationally mapped references.

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Cross-references

Sequence databases

AF170910 mRNA. Translation: AAD46682.1.
AC009853 Genomic DNA. Translation: AAF02166.1.
AK117215 mRNA. Translation: BAC41891.1.
BT005942 mRNA. Translation: AAO64877.1.
AJ222645 mRNA. Translation: CAA10905.1.
IPIIPI00530551.
RefSeqNP_187398.1.
UniGeneAt.24330

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGQ9SW95.

Proteomic databases

PRIDEQ9SW95.

Genome annotation databases

GeneID819930.
GenomeReviewsGene locus AT3G07420 in contig BA000014_GR.
KEGGath:AT3G07420.
NMPDRfig|3702.1.peg.12766.

Organism-specific databases

GeneFarm2802. 269.
TAIRAt3g07420.

Phylogenomic databases

OMACADEYFK.

Enzyme and pathway databases

BRENDA6.1.1.22. 302.

Gene expression databases

ArrayExpressQ9SW95.
GenevestigatorQ9SW95.
GermOnlineAT3G07420. Arabidopsis thaliana.

Family and domain databases

InterProIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
[Graphical view]
PANTHERPTHR22594. aa-tRNA-synt_II. 1 hit.
PfamPF00152. tRNA-synt_2. 1 hit.
[Graphical view]
PROSITEPS50862. AA_TRNA_LIGASE_II. False negative.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameSYNC2_ARATH
AccessionPrimary (citable) accession number: Q9SW95
Secondary accession number(s): O48594, Q8GZ48, Q9SRS4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: January 31, 2002
Last modified: November 3, 2009
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents