SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9SW18

- CHLM_ARATH

UniProt

Q9SW18 - CHLM_ARATH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Magnesium protoporphyrin IX methyltransferase, chloroplastic

Gene
CHLM, At4g25080, F13M23.220
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Converts Mg-protoporphyrin IX to Mg-protoporphyrin IX methylester using S-adenosyl-L-methionine as a cofactor. Involved in chloroplast-to-nucleus signaling by acting as a negative effector of nuclear photosynthetic gene expression.2 Publications

Catalytic activityi

S-adenosyl-L-methionine + magnesium protoporphyrin IX = S-adenosyl-L-homocysteine + magnesium protoporphyrin IX 13-methyl ester.1 Publication

Enzyme regulationi

Regulated by the folate status via an increased concentration of S-adenosyl-homocysteine (AdoHcy), a potent inhibitor of most AdoMet-dependent methyltransferases.1 Publication

Pathwayi

GO - Molecular functioni

  1. magnesium protoporphyrin IX methyltransferase activity Source: TAIR

GO - Biological processi

  1. chlorophyll biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Chlorophyll biosynthesis

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciARA:AT4G25080-MONOMER.
ARA:GQT-425-MONOMER.
ARA:GQT-426-MONOMER.
ARA:GQT-427-MONOMER.
ARA:GQT-428-MONOMER.
MetaCyc:AT4G25080-MONOMER.
UniPathwayiUPA00668.

Names & Taxonomyi

Protein namesi
Recommended name:
Magnesium protoporphyrin IX methyltransferase, chloroplastic (EC:2.1.1.11)
Gene namesi
Name:CHLM
Ordered Locus Names:At4g25080
ORF Names:F13M23.220
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 4

Organism-specific databases

TAIRiAT4G25080.

Subcellular locationi

Plastidchloroplast membrane; Peripheral membrane protein. Plastidchloroplast thylakoid membrane; Peripheral membrane protein 1 Publication

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast envelope Source: TAIR
  3. chloroplast membrane Source: UniProtKB-SubCell
  4. chloroplast thylakoid Source: TAIR
  5. chloroplast thylakoid membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Thylakoid

Pathology & Biotechi

Disruption phenotypei

Lethal under normal growth conditions and stunted albino plants unable to produce seeds when grown in presence of sucrose.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3939Chloroplast Reviewed predictionAdd
BLAST
Chaini40 – 312273Magnesium protoporphyrin IX methyltransferase, chloroplasticPRO_0000422668Add
BLAST

Proteomic databases

PRIDEiQ9SW18.

Expressioni

Inductioni

Up-regulated by light. Down-regulated by the herbicide R-imazethapyr.3 Publications

Gene expression databases

ArrayExpressiQ9SW18.
GenevestigatoriQ9SW18.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G25080.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9SW18.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG2227.
HOGENOMiHOG000154343.
InParanoidiQ9SW18.
KOiK03428.
OMAiKFEVNDL.
PhylomeDBiQ9SW18.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR010251. Mg_prot_MeTrfase.
IPR010940. Mg_prot_MeTrfase_C.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF07109. Mg-por_mtran_C. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR02021. BchM-ChlM. 1 hit.
PROSITEiPS51556. SAM_MT_MG_PIX. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9SW18-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MPFAPSLLSS SSSVSQFLPR FPNATRFNVT PRSRAATVVA ASVTDLAGVD    50
STTIAVLGGG SVAALAAMVS LTDPERRRKL QAEEVGGGDK EVVREYFNST 100
GFERWRKIYG ETDEVNRVQK DIRLGHAKTV ENTMLMLTED RSLAGVTVCD 150
AGCGTGLLSI PLAKEGAIVS ASDISAAMVA EAEMKAKAQL PSENLPKFEV 200
NDLESLTGKY DTVVCLDVLI HYPQNKADGM IAHLASLAEK RVILSFAPKT 250
FYYDILKRIG ELFPGPSKAT RAYLHSEADV ERALGKVGWK ISKRGLTTTQ 300
FYFSRLIEAV PM 312
Length:312
Mass (Da):33,796
Last modified:May 1, 2000 - v1
Checksum:i81A9C8611F2683A6
GO
Isoform 2 (identifier: Q9SW18-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-67: Missing.

Show »
Length:245
Mass (Da):27,148
Checksum:i255E601AB620DD8F
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 6767Missing in isoform 2. VSP_046549Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti106 – 1061R → G in BAH19415. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL035523 Genomic DNA. Translation: CAB36750.1.
AL161562 Genomic DNA. Translation: CAB79417.1.
CP002687 Genomic DNA. Translation: AEE85000.1.
CP002687 Genomic DNA. Translation: AEE85001.1.
CP002687 Genomic DNA. Translation: AEE85002.1.
CP002687 Genomic DNA. Translation: AEE85003.1.
CP002687 Genomic DNA. Translation: AEE85004.1.
AY034948 mRNA. Translation: AAK59454.1.
AY062991 mRNA. Translation: AAL34165.1.
AK316688 mRNA. Translation: BAH19415.1.
AK316669 mRNA. Translation: BAH19398.1.
AK319071 mRNA. Translation: BAH57186.1.
PIRiT05529.
RefSeqiNP_001119052.1. NM_001125580.1. [Q9SW18-2]
NP_001190832.1. NM_001203903.1. [Q9SW18-1]
NP_194238.1. NM_118640.2. [Q9SW18-1]
NP_849438.1. NM_179107.1. [Q9SW18-1]
NP_849439.1. NM_179108.2. [Q9SW18-1]
UniGeneiAt.22023.

Genome annotation databases

EnsemblPlantsiAT4G25080.1; AT4G25080.1; AT4G25080. [Q9SW18-1]
AT4G25080.2; AT4G25080.2; AT4G25080. [Q9SW18-1]
AT4G25080.3; AT4G25080.3; AT4G25080. [Q9SW18-1]
AT4G25080.5; AT4G25080.5; AT4G25080. [Q9SW18-1]
GeneIDi828611.
KEGGiath:AT4G25080.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL035523 Genomic DNA. Translation: CAB36750.1 .
AL161562 Genomic DNA. Translation: CAB79417.1 .
CP002687 Genomic DNA. Translation: AEE85000.1 .
CP002687 Genomic DNA. Translation: AEE85001.1 .
CP002687 Genomic DNA. Translation: AEE85002.1 .
CP002687 Genomic DNA. Translation: AEE85003.1 .
CP002687 Genomic DNA. Translation: AEE85004.1 .
AY034948 mRNA. Translation: AAK59454.1 .
AY062991 mRNA. Translation: AAL34165.1 .
AK316688 mRNA. Translation: BAH19415.1 .
AK316669 mRNA. Translation: BAH19398.1 .
AK319071 mRNA. Translation: BAH57186.1 .
PIRi T05529.
RefSeqi NP_001119052.1. NM_001125580.1. [Q9SW18-2 ]
NP_001190832.1. NM_001203903.1. [Q9SW18-1 ]
NP_194238.1. NM_118640.2. [Q9SW18-1 ]
NP_849438.1. NM_179107.1. [Q9SW18-1 ]
NP_849439.1. NM_179108.2. [Q9SW18-1 ]
UniGenei At.22023.

3D structure databases

ProteinModelPortali Q9SW18.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT4G25080.1-P.

Proteomic databases

PRIDEi Q9SW18.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT4G25080.1 ; AT4G25080.1 ; AT4G25080 . [Q9SW18-1 ]
AT4G25080.2 ; AT4G25080.2 ; AT4G25080 . [Q9SW18-1 ]
AT4G25080.3 ; AT4G25080.3 ; AT4G25080 . [Q9SW18-1 ]
AT4G25080.5 ; AT4G25080.5 ; AT4G25080 . [Q9SW18-1 ]
GeneIDi 828611.
KEGGi ath:AT4G25080.

Organism-specific databases

TAIRi AT4G25080.

Phylogenomic databases

eggNOGi COG2227.
HOGENOMi HOG000154343.
InParanoidi Q9SW18.
KOi K03428.
OMAi KFEVNDL.
PhylomeDBi Q9SW18.

Enzyme and pathway databases

UniPathwayi UPA00668 .
BioCyci ARA:AT4G25080-MONOMER.
ARA:GQT-425-MONOMER.
ARA:GQT-426-MONOMER.
ARA:GQT-427-MONOMER.
ARA:GQT-428-MONOMER.
MetaCyc:AT4G25080-MONOMER.

Gene expression databases

ArrayExpressi Q9SW18.
Genevestigatori Q9SW18.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR010251. Mg_prot_MeTrfase.
IPR010940. Mg_prot_MeTrfase_C.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF07109. Mg-por_mtran_C. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR02021. BchM-ChlM. 1 hit.
PROSITEi PS51556. SAM_MT_MG_PIX. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  4. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
    Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
    DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: cv. Columbia.
  5. "The plant S-adenosyl-L-methionine:Mg-protoporphyrin IX methyltransferase is located in both envelope and thylakoid chloroplast membranes."
    Block M.A., Tewari A.K., Albrieux C., Marechal E., Joyard J.
    Eur. J. Biochem. 269:240-248(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, TOPOLOGY.
  6. "Knock-out of the magnesium protoporphyrin IX methyltransferase gene in Arabidopsis. Effects on chloroplast development and on chloroplast-to-nucleus signaling."
    Pontier D., Albrieux C., Joyard J., Lagrange T., Block M.A.
    J. Biol. Chem. 282:2297-2304(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    Strain: cv. Wassilewskija.
  7. "Light signalling pathways regulating the Mg-chelatase branchpoint of chlorophyll synthesis during de-etiolation in Arabidopsis thaliana."
    Stephenson P.G., Terry M.J.
    Photochem. Photobiol. Sci. 7:1243-1252(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY LIGHT.
  8. "C1 metabolism and chlorophyll synthesis: the Mg-protoporphyrin IX methyltransferase activity is dependent on the folate status."
    Van Wilder V., De Brouwer V., Loizeau K., Gambonnet B., Albrieux C., Van Der Straeten D., Lambert W.E., Douce R., Block M.A., Rebeille F., Ravanel S.
    New Phytol. 182:137-145(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION.
  9. "Imazethapyr enantioselectively affects chlorophyll synthesis and photosynthesis in Arabidopsis thaliana."
    Qian H., Han X., Zhang Q., Sun Z., Sun L., Fu Z.
    J. Agric. Food Chem. 61:1172-1178(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY IMAZETHAPYR.

Entry informationi

Entry nameiCHLM_ARATH
AccessioniPrimary (citable) accession number: Q9SW18
Secondary accession number(s): B3H4K6, B9DF98
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2013
Last sequence update: May 1, 2000
Last modified: June 11, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi