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Protein

Magnesium protoporphyrin IX methyltransferase, chloroplastic

Gene

CHLM

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Converts Mg-protoporphyrin IX to Mg-protoporphyrin IX methylester using S-adenosyl-L-methionine as a cofactor. Involved in chloroplast-to-nucleus signaling by acting as a negative effector of nuclear photosynthetic gene expression.2 Publications

Catalytic activityi

S-adenosyl-L-methionine + magnesium protoporphyrin IX = S-adenosyl-L-homocysteine + magnesium protoporphyrin IX 13-methyl ester.PROSITE-ProRule annotation1 Publication

Enzyme regulationi

Regulated by the folate status via an increased concentration of S-adenosyl-homocysteine (AdoHcy), a potent inhibitor of most AdoMet-dependent methyltransferases.1 Publication

Pathwayi: chlorophyll biosynthesis

This protein is involved in the pathway chlorophyll biosynthesis, which is part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the pathway chlorophyll biosynthesis and in Porphyrin-containing compound metabolism.

GO - Molecular functioni

  • magnesium protoporphyrin IX methyltransferase activity Source: TAIR

GO - Biological processi

  • chlorophyll biosynthetic process Source: CACAO

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processChlorophyll biosynthesis
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciARA:AT4G25080-MONOMER.
ARA:GQT-425-MONOMER.
MetaCyc:AT4G25080-MONOMER.
UniPathwayiUPA00668.

Names & Taxonomyi

Protein namesi
Recommended name:
Magnesium protoporphyrin IX methyltransferase, chloroplastic (EC:2.1.1.11)
Gene namesi
Name:CHLM
Ordered Locus Names:At4g25080
ORF Names:F13M23.220
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G25080.
TAIRilocus:2117388. AT4G25080.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Thylakoid

Pathology & Biotechi

Disruption phenotypei

Lethal under normal growth conditions and stunted albino plants unable to produce seeds when grown in presence of sucrose.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 39ChloroplastSequence analysisAdd BLAST39
ChainiPRO_000042266840 – 312Magnesium protoporphyrin IX methyltransferase, chloroplasticAdd BLAST273

Proteomic databases

PaxDbiQ9SW18.
PRIDEiQ9SW18.

Expressioni

Inductioni

Up-regulated by light. Down-regulated by the herbicide R-imazethapyr.2 Publications

Gene expression databases

ExpressionAtlasiQ9SW18. baseline and differential.
GenevisibleiQ9SW18. AT.

Interactioni

Protein-protein interaction databases

BioGridi13898. 2 interactors.
STRINGi3702.AT4G25080.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SW18.
SMRiQ9SW18.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Magnesium protoporphyrin O-methyltransferase family.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1270. Eukaryota.
COG2227. LUCA.
HOGENOMiHOG000154343.
InParanoidiQ9SW18.
KOiK03428.
OMAiLDVFIHY.
OrthoDBiEOG09360INC.
PhylomeDBiQ9SW18.

Family and domain databases

InterProiView protein in InterPro
IPR010251. Mg_prot_MeTrfase.
IPR010940. Mg_prot_MeTrfase_C.
IPR029063. SAM-dependent_MTases.
PANTHERiPTHR43591:SF9. PTHR43591:SF9. 1 hit.
PfamiView protein in Pfam
PF07109. Mg-por_mtran_C. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR02021. BchM-ChlM. 1 hit.
PROSITEiView protein in PROSITE
PS51556. SAM_MT_MG_PIX. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9SW18-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPFAPSLLSS SSSVSQFLPR FPNATRFNVT PRSRAATVVA ASVTDLAGVD
60 70 80 90 100
STTIAVLGGG SVAALAAMVS LTDPERRRKL QAEEVGGGDK EVVREYFNST
110 120 130 140 150
GFERWRKIYG ETDEVNRVQK DIRLGHAKTV ENTMLMLTED RSLAGVTVCD
160 170 180 190 200
AGCGTGLLSI PLAKEGAIVS ASDISAAMVA EAEMKAKAQL PSENLPKFEV
210 220 230 240 250
NDLESLTGKY DTVVCLDVLI HYPQNKADGM IAHLASLAEK RVILSFAPKT
260 270 280 290 300
FYYDILKRIG ELFPGPSKAT RAYLHSEADV ERALGKVGWK ISKRGLTTTQ
310
FYFSRLIEAV PM
Length:312
Mass (Da):33,796
Last modified:May 1, 2000 - v1
Checksum:i81A9C8611F2683A6
GO
Isoform 2 (identifier: Q9SW18-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-67: Missing.

Show »
Length:245
Mass (Da):27,148
Checksum:i255E601AB620DD8F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti106R → G in BAH19415 (PubMed:19423640).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0465491 – 67Missing in isoform 2. 1 PublicationAdd BLAST67

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL035523 Genomic DNA. Translation: CAB36750.1.
AL161562 Genomic DNA. Translation: CAB79417.1.
CP002687 Genomic DNA. Translation: AEE85000.1.
CP002687 Genomic DNA. Translation: AEE85001.1.
CP002687 Genomic DNA. Translation: AEE85002.1.
CP002687 Genomic DNA. Translation: AEE85003.1.
CP002687 Genomic DNA. Translation: AEE85004.1.
AY034948 mRNA. Translation: AAK59454.1.
AY062991 mRNA. Translation: AAL34165.1.
AK316688 mRNA. Translation: BAH19415.1.
AK316669 mRNA. Translation: BAH19398.1.
AK319071 mRNA. Translation: BAH57186.1.
PIRiT05529.
RefSeqiNP_001119052.1. NM_001125580.1. [Q9SW18-2]
NP_001190832.1. NM_001203903.1. [Q9SW18-1]
NP_194238.1. NM_118640.3. [Q9SW18-1]
NP_849438.1. NM_179107.2. [Q9SW18-1]
NP_849439.1. NM_179108.3. [Q9SW18-1]
UniGeneiAt.22023.

Genome annotation databases

EnsemblPlantsiAT4G25080.1; AT4G25080.1; AT4G25080. [Q9SW18-1]
AT4G25080.2; AT4G25080.2; AT4G25080. [Q9SW18-1]
AT4G25080.3; AT4G25080.3; AT4G25080. [Q9SW18-1]
AT4G25080.4; AT4G25080.4; AT4G25080. [Q9SW18-2]
AT4G25080.5; AT4G25080.5; AT4G25080. [Q9SW18-1]
GeneIDi828611.
GrameneiAT4G25080.1; AT4G25080.1; AT4G25080.
AT4G25080.2; AT4G25080.2; AT4G25080.
AT4G25080.3; AT4G25080.3; AT4G25080.
AT4G25080.4; AT4G25080.4; AT4G25080.
AT4G25080.5; AT4G25080.5; AT4G25080.
KEGGiath:AT4G25080.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCHLM_ARATH
AccessioniPrimary (citable) accession number: Q9SW18
Secondary accession number(s): B3H4K6, B9DF98
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 26, 2013
Last sequence update: May 1, 2000
Last modified: November 22, 2017
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families