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Q9SW18 (CHLM_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Magnesium protoporphyrin IX methyltransferase, chloroplastic

EC=2.1.1.11
Gene names
Name:CHLM
Ordered Locus Names:At4g25080
ORF Names:F13M23.220
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length312 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Converts Mg-protoporphyrin IX to Mg-protoporphyrin IX methylester using S-adenosyl-L-methionine as a cofactor. Involved in chloroplast-to-nucleus signaling by acting as a negative effector of nuclear photosynthetic gene expression. Ref.5 Ref.6

Catalytic activity

S-adenosyl-L-methionine + magnesium protoporphyrin IX = S-adenosyl-L-homocysteine + magnesium protoporphyrin IX 13-methyl ester. Ref.5

Enzyme regulation

Regulated by the folate status via an increased concentration of S-adenosyl-homocysteine (AdoHcy), a potent inhibitor of most AdoMet-dependent methyltransferases. Ref.8

Pathway

Porphyrin-containing compound metabolism; chlorophyll biosynthesis.

Subcellular location

Plastidchloroplast membrane; Peripheral membrane protein. Plastidchloroplast thylakoid membrane; Peripheral membrane protein Ref.5.

Induction

Up-regulated by light. Down-regulated by the herbicide R-imazethapyr. Ref.7 Ref.8 Ref.9

Disruption phenotype

Lethal under normal growth conditions and stunted albino plants unable to produce seeds when grown in presence of sucrose. Ref.6

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. Magnesium protoporphyrin O-methyltransferase family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9SW18-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9SW18-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-67: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3939Chloroplast Potential
Chain40 – 312273Magnesium protoporphyrin IX methyltransferase, chloroplastic
PRO_0000422668

Natural variations

Alternative sequence1 – 6767Missing in isoform 2.
VSP_046549

Experimental info

Sequence conflict1061R → G in BAH19415. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 81A9C8611F2683A6

FASTA31233,796
        10         20         30         40         50         60 
MPFAPSLLSS SSSVSQFLPR FPNATRFNVT PRSRAATVVA ASVTDLAGVD STTIAVLGGG 

        70         80         90        100        110        120 
SVAALAAMVS LTDPERRRKL QAEEVGGGDK EVVREYFNST GFERWRKIYG ETDEVNRVQK 

       130        140        150        160        170        180 
DIRLGHAKTV ENTMLMLTED RSLAGVTVCD AGCGTGLLSI PLAKEGAIVS ASDISAAMVA 

       190        200        210        220        230        240 
EAEMKAKAQL PSENLPKFEV NDLESLTGKY DTVVCLDVLI HYPQNKADGM IAHLASLAEK 

       250        260        270        280        290        300 
RVILSFAPKT FYYDILKRIG ELFPGPSKAT RAYLHSEADV ERALGKVGWK ISKRGLTTTQ 

       310 
FYFSRLIEAV PM 

« Hide

Isoform 2 [UniParc].

Checksum: 255E601AB620DD8F
Show »

FASTA24527,148

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. expand/collapse author list , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[4]"Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: cv. Columbia.
[5]"The plant S-adenosyl-L-methionine:Mg-protoporphyrin IX methyltransferase is located in both envelope and thylakoid chloroplast membranes."
Block M.A., Tewari A.K., Albrieux C., Marechal E., Joyard J.
Eur. J. Biochem. 269:240-248(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, TOPOLOGY.
[6]"Knock-out of the magnesium protoporphyrin IX methyltransferase gene in Arabidopsis. Effects on chloroplast development and on chloroplast-to-nucleus signaling."
Pontier D., Albrieux C., Joyard J., Lagrange T., Block M.A.
J. Biol. Chem. 282:2297-2304(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
Strain: cv. Wassilewskija.
[7]"Light signalling pathways regulating the Mg-chelatase branchpoint of chlorophyll synthesis during de-etiolation in Arabidopsis thaliana."
Stephenson P.G., Terry M.J.
Photochem. Photobiol. Sci. 7:1243-1252(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY LIGHT.
[8]"C1 metabolism and chlorophyll synthesis: the Mg-protoporphyrin IX methyltransferase activity is dependent on the folate status."
Van Wilder V., De Brouwer V., Loizeau K., Gambonnet B., Albrieux C., Van Der Straeten D., Lambert W.E., Douce R., Block M.A., Rebeille F., Ravanel S.
New Phytol. 182:137-145(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ENZYME REGULATION.
[9]"Imazethapyr enantioselectively affects chlorophyll synthesis and photosynthesis in Arabidopsis thaliana."
Qian H., Han X., Zhang Q., Sun Z., Sun L., Fu Z.
J. Agric. Food Chem. 61:1172-1178(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY IMAZETHAPYR.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL035523 Genomic DNA. Translation: CAB36750.1.
AL161562 Genomic DNA. Translation: CAB79417.1.
CP002687 Genomic DNA. Translation: AEE85000.1.
CP002687 Genomic DNA. Translation: AEE85001.1.
CP002687 Genomic DNA. Translation: AEE85002.1.
CP002687 Genomic DNA. Translation: AEE85003.1.
CP002687 Genomic DNA. Translation: AEE85004.1.
AY034948 mRNA. Translation: AAK59454.1.
AY062991 mRNA. Translation: AAL34165.1.
AK316688 mRNA. Translation: BAH19415.1.
AK316669 mRNA. Translation: BAH19398.1.
AK319071 mRNA. Translation: BAH57186.1.
PIRT05529.
RefSeqNP_001119052.1. NM_001125580.1.
NP_001190832.1. NM_001203903.1.
NP_194238.1. NM_118640.2.
NP_849438.1. NM_179107.1.
NP_849439.1. NM_179108.2.
UniGeneAt.22023.

3D structure databases

ProteinModelPortalQ9SW18.
SMRQ9SW18. Positions 102-295.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT4G25080.1-P.

Proteomic databases

PRIDEQ9SW18.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT4G25080.1; AT4G25080.1; AT4G25080. [Q9SW18-1]
AT4G25080.2; AT4G25080.2; AT4G25080. [Q9SW18-1]
AT4G25080.3; AT4G25080.3; AT4G25080. [Q9SW18-1]
AT4G25080.5; AT4G25080.5; AT4G25080. [Q9SW18-1]
GeneID828611.
KEGGath:AT4G25080.

Organism-specific databases

TAIRAT4G25080.

Phylogenomic databases

eggNOGCOG2227.
HOGENOMHOG000154343.
InParanoidQ9SW18.
KOK03428.
OMALDVFIHY.
PhylomeDBQ9SW18.
ProtClustDBPLN02585.

Enzyme and pathway databases

BioCycARA:AT4G25080-MONOMER.
ARA:GQT-425-MONOMER.
ARA:GQT-426-MONOMER.
ARA:GQT-427-MONOMER.
ARA:GQT-428-MONOMER.
MetaCyc:AT4G25080-MONOMER.
UniPathwayUPA00668.

Gene expression databases

ArrayExpressQ9SW18.
GenevestigatorQ9SW18.

Family and domain databases

InterProIPR010251. Mg_prot_MeTrfase.
IPR010940. Mg_prot_MeTrfase_C.
[Graphical view]
PfamPF07109. Mg-por_mtran_C. 1 hit.
[Graphical view]
TIGRFAMsTIGR02021. BchM-ChlM. 1 hit.
PROSITEPS51556. SAM_MT_MG_PIX. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCHLM_ARATH
AccessionPrimary (citable) accession number: Q9SW18
Secondary accession number(s): B3H4K6, B9DF98
Entry history
Integrated into UniProtKB/Swiss-Prot: June 26, 2013
Last sequence update: May 1, 2000
Last modified: April 16, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names