Reviewed, UniProtKB/Swiss-Prot Q9SW11 (PUB35_ARATH)

Names and origin

Protein names

Recommended name:
    U-box domain-containing protein 35
Alternative name(s):
    Plant U-box protein 35
Including the following 2 domains:
    1- Recommended name:
            E3 ubiquitin ligase
              EC=6.3.2.-
    2- Recommended name:
            Serine/threonine-protein kinase
              EC=2.7.11.-

Gene names

Name:	PUB35
Ordered Locus Names:	At4g25160
ORF Names:	F24A6.13, F13M23.300

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	835 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Functions as an E3 ubiquitin ligase By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Pathway	Protein modification; protein ubiquitination.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain. Contains 1 U-box domain.
Sequence caution	The sequence BX828107 differs from that shown. Reason: Frameshift at position 728. The sequence CAB36758.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAB79425.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Ligase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro protein ubiquitination Inferred from electronic annotation. Source: InterPro response to stress Inferred from electronic annotation. Source: InterPro
Cellular component	ubiquitin ligase complex Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
At5g64330	Q0WSP1	1	EBI-626181,EBI-1553842

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 835	835	U-box domain-containing protein 35		PRO_0000322142
Regions
	Domain	480 – 745	266	Protein kinase
	Domain	767 – 835	69	U-box
	Nucleotide binding	486 – 494	9	ATP By similarity
	Coiled coil	340 – 459	120	Potential
	Compositional bias	11 – 18	8	Poly-Pro
Sites
	Active site	602	1	Proton acceptor By similarity
	Binding site	507	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9SW11-1 [UniParc]. Last modified February 26, 2008. Version 2. Checksum: 2BC570AB1A87024C	FASTA	835	93,754
	10 20 30 40 50 60 MSRSPDKLAL PPPPPPPPSR TVVVALSGSS KSKYVVTWAI EKFATEGNVG FKLLHIHPMI 70 80 90 100 110 120 TSVPTPMGNA IPISEVRDDV VTAYRQEILW QSEEMLKPYT KLFVRRKVAV EVLVIESDNV 130 140 150 160 170 180 AAAIAEEVTR DSIDRIVIGG SSRSFFSRKA DICSVISALM PNFCTVYVVS KGKLSCVRPS 190 200 210 220 230 240 DSDGNATIRE DGSERTNSSS GSSGPTSDSS DVMSSAHDSQ SRPLSLPVRR MQHFPAIAGQ 250 260 270 280 290 300 ASVPMETSSV GSDETRCMSL DAEEARDVSS INRSSTDTTS RWTPRRRDYE ERKEAMSSSS 310 320 330 340 350 360 SNREYGNFGT RFSWSGMGVD TTHSRASQQA SNMSDALSEQ SYTDNQVNLN FEVEKLRAEL 370 380 390 400 410 420 RHVQEMYAVA QTETFDASRK LGELNQRRLE EAIKLEELKL KEYEARELAE KEKQNFEKAR 430 440 450 460 470 480 RDAESMRERA EREIAQRREA ERKSARDTKE KEKLEGTLGS PQLQYQHFAW EEIMAATSSF 490 500 510 520 530 540 SEELKIGMGA YGAVYKCNLH HTTAVVKVLQ SAENQLSKQF QQELEILSKI RHPHLVLLLG 550 560 570 580 590 600 ACPEQGALVY EYMENGSLED RLFQVNNSPP LPWFERFRIA WEVAAALVFL HKSKPKPIIH 610 620 630 640 650 660 RDLKPANILL DHNFVSKVGD VGLSTMVQVD PLSTKFTIYK QTSPVGTLCY IDPEYQRTGR 670 680 690 700 710 720 ISSKSDIYSF GMILLQLLTA KPAIALTHFV ESAMDSNDEF LKILDQKAGN WPIEETRELA 730 740 750 760 770 780 ALALCCTELR GKDRPDLKDQ ILPALENLKK VAEKARNSFS GVSTQPPTHF ICPLLKDVMN 790 800 810 820 830 EPCVAADGYT YDRHAIEEWL KEHNTSPMTD SPLHSKNLLP NYTLYTAIME WRSTR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation." Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M. Genome Res. 14:406-413(2004) [PubMed: 14993207] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"The U-box protein family in plants." Azevedo C., Santos-Rosa M.J., Shirasu K. Trends Plant Sci. 6:354-358(2001) [PubMed: 11495788] [Abstract] Cited for: GENE FAMILY ORGANIZATION, NOMENCLATURE.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL035396 Genomic DNA. No translation available. AL035523 Genomic DNA. Translation: CAB36758.1. Sequence problems. AL161562 Genomic DNA. Translation: CAB79425.1. Sequence problems. BX828107 mRNA. No translation available.
IPI	IPI00540343.
PIR	T05537.
RefSeq	NP_194246.2.
UniGene	At.32287
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9SW11. 1 interaction.
Proteomic databases
PRIDE	Q9SW11.
Genome annotation databases
GeneID	828619.
GenomeReviews	Gene locus AT4G25160 in contig CT486007_GR.
KEGG	ath:AT4G25160.
Organism-specific databases
GeneFarm	2015. 148.
TAIR	At4g25160.
Phylogenomic databases
OMA	Q9SW11. FRIAWEV.
Gene expression databases
ArrayExpress	Q9SW11.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR014729. Rossmann-like_a/b/a_fold. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR003613. Ubox_domain. IPR006016. UspA. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. PF04564. U-box. 1 hit. PF00582. Usp. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00504. Ubox. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUB35_ARATH

Accession

Primary (citable) accession number: Q9SW11

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	February 26, 2008
Last modified:	June 16, 2009
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)