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Protein

DNA-directed RNA polymerase I subunit 1

Gene

NRPA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from NRPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition.By similarity

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi79Zinc 1By similarity1
Metal bindingi82Zinc 1By similarity1
Metal bindingi89Zinc 1By similarity1
Metal bindingi92Zinc 1; via tele nitrogenBy similarity1
Metal bindingi119Zinc 2By similarity1
Metal bindingi122Zinc 2By similarity1
Metal bindingi213Zinc 2By similarity1
Metal bindingi216Zinc 2By similarity1
Metal bindingi602Magnesium; catalyticBy similarity1
Metal bindingi604Magnesium; catalyticBy similarity1
Metal bindingi606Magnesium; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Ribosome biogenesis, Transcription

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase I subunit 1Curated
Alternative name(s):
DNA-directed RNA polymerase I subunit RPA1Curated (EC:2.7.7.6Curated)
Short name:
DNA polymerase I subunit A1Curated
Nuclear RNA polymerase A1Imported
Gene namesi
Name:NRPA1Imported
Synonyms:RPA1Curated
Ordered Locus Names:At3g57660Imported
ORF Names:F15B8.150Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G57660.

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

  • DNA-directed RNA polymerase I complex Source: GO_Central
  • nucleus Source: TAIR
  • plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004340081 – 1670DNA-directed RNA polymerase I subunit 1Add BLAST1670

Proteomic databases

PaxDbiQ9SVY0.
PRIDEiQ9SVY0.

PTM databases

iPTMnetiQ9SVY0.

Expressioni

Gene expression databases

GenevisibleiQ9SVY0. AT.

Interactioni

Subunit structurei

Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits.By similarity

Protein-protein interaction databases

IntActiQ9SVY0. 1 interactor.
STRINGi3702.AT3G57660.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SVY0.
SMRiQ9SVY0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1005 – 1017Bridging helixCuratedAdd BLAST13

Sequence similaritiesi

Belongs to the RNA polymerase beta' chain family.Curated

Phylogenomic databases

eggNOGiKOG0262. Eukaryota.
COG0086. LUCA.
HOGENOMiHOG000205401.
KOiK02999.
OMAiIREINHV.
OrthoDBiEOG093602I5.
PhylomeDBiQ9SVY0.

Family and domain databases

InterProiIPR015699. DNA-dir_RNA_pol1_lsu.
IPR000722. RNA_pol_asu.
IPR006592. RNA_pol_N.
IPR007080. RNA_pol_Rpb1_1.
IPR007066. RNA_pol_Rpb1_3.
IPR007083. RNA_pol_Rpb1_4.
IPR007081. RNA_pol_Rpb1_5.
[Graphical view]
PANTHERiPTHR19376:SF11. PTHR19376:SF11. 4 hits.
PfamiPF04997. RNA_pol_Rpb1_1. 1 hit.
PF00623. RNA_pol_Rpb1_2. 1 hit.
PF04983. RNA_pol_Rpb1_3. 1 hit.
PF05000. RNA_pol_Rpb1_4. 1 hit.
PF04998. RNA_pol_Rpb1_5. 1 hit.
[Graphical view]
SMARTiSM00663. RPOLA_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SVY0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHAQTTEVC LSFHRSLLFP MGASQVVESV RFSFMTEQDV RKHSFLKVTS
60 70 80 90 100
PILHDNVGNP FPGGLYDLKL GPKDDKQACN SCGQLKLACP GHCGHIELVF
110 120 130 140 150
PIYHPLLFNL LFNFLQRACF FCHHFMAKPE DVERAVSQLK LIIKGDIVSA
160 170 180 190 200
KQLESNTPTK SKSSDESCES VVTTDSSEEC EDSDVEDQRW TSLQFAEVTA
210 220 230 240 250
VLKNFMRLSS KSCSRCKGIN PKLEKPMFGW VRMRAMKDSD VGANVIRGLK
260 270 280 290 300
LKKSTSSVEN PDGFDDSGID ALSEVEDGDK ETREKSTEVA AEFEEHNSKR
310 320 330 340 350
DLLPSEVRNI LKHLWQNEHE FCSFIGDLWQ SGSEKIDYSM FFLESVLVPP
360 370 380 390 400
TKFRPPTTGG DSVMEHPQTV GLNKVIESNN ILGNACTNKL DQSKVIFRWR
410 420 430 440 450
NLQESVNVLF DSKTATVQSQ RDSSGICQLL EKKEGLFRQK MMGKRVNHAC
460 470 480 490 500
RSVISPDPYI AVNDIGIPPC FALKLTYPER VTPWNVEKLR EAIINGPDIH
510 520 530 540 550
PGATHYSDKS STMKLPSTEK ARRAIARKLL SSRGATTELG KTCDINFEGK
560 570 580 590 600
TVHRHMRDGD IVLVNRQPTL HKPSLMAHKV RVLKGEKTLR LHYANCSTYN
610 620 630 640 650
ADFDGDEMNV HFPQDEISRA EAYNIVNANN QYARPSNGEP LRALIQDHIV
660 670 680 690 700
SSVLLTKRDT FLDKDHFNQL LFSSGVTDMV LSTFSGRSGK KVMVSASDAE
710 720 730 740 750
LLTVTPAILK PVPLWTGKQV ITAVLNQITK GHPPFTVEKA TKLPVDFFKC
760 770 780 790 800
RSREVKPNSG DLTKKKEIDE SWKQNLNEDK LHIRKNEFVC GVIDKAQFAD
810 820 830 840 850
YGLVHTVHEL YGSNAAGNLL SVFSRLFTVF LQTHGFTCGV DDLIILKDMD
860 870 880 890 900
EERTKQLQEC ENVGERVLRK TFGIDVDVQI DPQDMRSRIE RILYEDGESA
910 920 930 940 950
LASLDRSIVN YLNQCSSKGV MNDLLSDGLL KTPGRNCISL MTISGAKGSK
960 970 980 990 1000
VNFQQISSHL GQQDLEGKRV PRMVSGKTLP CFHPWDWSPR AGGFISDRFL
1010 1020 1030 1040 1050
SGLRPQEYYF HCMAGREGLV DTAVKTSRSG YLQRCLMKNL ESLKVNYDCT
1060 1070 1080 1090 1100
VRDADGSIIQ FQYGEDGVDV HRSSFIEKFK ELTINQDMVL QKCSEDMLSG
1110 1120 1130 1140 1150
ASSYISDLPI SLKKGAEKFV EAMPMNERIA SKFVRQEELL KLVKSKFFAS
1160 1170 1180 1190 1200
LAQPGEPVGV LAAQSVGEPS TQMTLNTFHL AGRGEMNVTL GIPRLQEILM
1210 1220 1230 1240 1250
TAAANIKTPI MTCPLLKGKT KEDANDITDR LRKITVADII KSMELSVVPY
1260 1270 1280 1290 1300
TVYENEVCSI HKLKINLYKP EHYPKHTDIT EEDWEETMRA VFLRKLEDAI
1310 1320 1330 1340 1350
ETHMKMLHRI RGIHNDVTGP IAGNETDNDD SVSGKQNEDD GDDDGEGTEV
1360 1370 1380 1390 1400
DDLGSDAQKQ KKQETDEMDY EENSEDETNE PSSISGVEDP EMDSENEDTE
1410 1420 1430 1440 1450
VSKEDTPEPQ EESMEPQKEV KGVKNVKEQS KKKRRKFVRA KSDRHIFVKG
1460 1470 1480 1490 1500
EGEKFEVHFK FATDDPHILL AQIAQQTAQK VYIQNSGKIE RCTVANCGDP
1510 1520 1530 1540 1550
QVIYHGDNPK ERREISNDEK KASPALHASG VDFPALWEFQ DKLDVRYLYS
1560 1570 1580 1590 1600
NSIHDMLNIF GVEAARETII REINHVFKSY GISVSIRHLN LIADYMTFSG
1610 1620 1630 1640 1650
GYRPMSRMGG IAESTSPFCR MTFETATKFI VQAATYGEKD TLETPSARIC
1660 1670
LGLPALSGTG CFDLMQRVEL
Length:1,670
Mass (Da):187,576
Last modified:May 1, 2000 - v1
Checksum:i2F43F2907D4B2C8F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL049660 Genomic DNA. Translation: CAB41189.1.
CP002686 Genomic DNA. Translation: AEE79684.1.
PIRiT06754.
RefSeqiNP_191325.1. NM_115626.1.
UniGeneiAt.50286.

Genome annotation databases

EnsemblPlantsiAT3G57660.1; AT3G57660.1; AT3G57660.
GeneIDi824935.
GrameneiAT3G57660.1; AT3G57660.1; AT3G57660.
KEGGiath:AT3G57660.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL049660 Genomic DNA. Translation: CAB41189.1.
CP002686 Genomic DNA. Translation: AEE79684.1.
PIRiT06754.
RefSeqiNP_191325.1. NM_115626.1.
UniGeneiAt.50286.

3D structure databases

ProteinModelPortaliQ9SVY0.
SMRiQ9SVY0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9SVY0. 1 interactor.
STRINGi3702.AT3G57660.1.

PTM databases

iPTMnetiQ9SVY0.

Proteomic databases

PaxDbiQ9SVY0.
PRIDEiQ9SVY0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G57660.1; AT3G57660.1; AT3G57660.
GeneIDi824935.
GrameneiAT3G57660.1; AT3G57660.1; AT3G57660.
KEGGiath:AT3G57660.

Organism-specific databases

TAIRiAT3G57660.

Phylogenomic databases

eggNOGiKOG0262. Eukaryota.
COG0086. LUCA.
HOGENOMiHOG000205401.
KOiK02999.
OMAiIREINHV.
OrthoDBiEOG093602I5.
PhylomeDBiQ9SVY0.

Miscellaneous databases

PROiQ9SVY0.

Gene expression databases

GenevisibleiQ9SVY0. AT.

Family and domain databases

InterProiIPR015699. DNA-dir_RNA_pol1_lsu.
IPR000722. RNA_pol_asu.
IPR006592. RNA_pol_N.
IPR007080. RNA_pol_Rpb1_1.
IPR007066. RNA_pol_Rpb1_3.
IPR007083. RNA_pol_Rpb1_4.
IPR007081. RNA_pol_Rpb1_5.
[Graphical view]
PANTHERiPTHR19376:SF11. PTHR19376:SF11. 4 hits.
PfamiPF04997. RNA_pol_Rpb1_1. 1 hit.
PF00623. RNA_pol_Rpb1_2. 1 hit.
PF04983. RNA_pol_Rpb1_3. 1 hit.
PF05000. RNA_pol_Rpb1_4. 1 hit.
PF04998. RNA_pol_Rpb1_5. 1 hit.
[Graphical view]
SMARTiSM00663. RPOLA_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNRPA1_ARATH
AccessioniPrimary (citable) accession number: Q9SVY0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 14, 2015
Last sequence update: May 1, 2000
Last modified: November 2, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.