Q9SVS8 (G3OX3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gibberellin 3-beta-dioxygenase 3
EC=1.14.11.15

Alternative name(s):
GA 3-oxidase 3
Short name=AtGA3ox3
Short name=AtGA3ox4
Gibberellin 3 beta-hydroxylase 3

Gene names

Ordered Locus Names:	At4g21690
ORF Names:	F17L22.150

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Converts the inactive gibbberellin precursors GA9 and GA20 in the bioactives gibberellins GA4 and GA1 By similarity.
Catalytic activity	Gibberellin 20 + 2-oxoglutarate + O2 = gibberellin 1 + succinate + CO2.
Cofactor	Ascorbate By similarity. Iron By similarity.
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family. GA3OX subfamily. Contains 1 Fe2OG dioxygenase domain.

Ontologies

Keywords
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	gibberellin 3-beta-dioxygenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 349	349	Gibberellin 3-beta-dioxygenase 3		PRO_0000067315
Regions
	Domain	201 – 305	105	Fe2OG dioxygenase
Sites
	Active site	296	1	Potential
	Metal binding	226	1	Iron By similarity
	Metal binding	228	1	Iron By similarity
	Metal binding	286	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9SVS8 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 7A8F92BC4816CFFC	FASTA	349	39,211
	10 20 30 40 50 60 MSSVTQLFKN NPVNRDRIIP LDFTNTKTLP DSHVWSKPEP ETTSGPIPVI SLSNPEEHGL 70 80 90 100 110 120 LRQACEEWGV FHITDHGVSH SLLHNVDCQM KRLFSLPMHR KILAVRSPDE STGYGVVRIS 130 140 150 160 170 180 MFYDKLMWSE GFSVMGSSLR RHATLLWPDD HAEFCNVMEE YQKAMDDLSH RLISMLMGSL 190 200 210 220 230 240 GLTHEDLGWL VPDKTGSGTD SIQSFLQLNS YPVCPDPHLA MGLAPHTDSS LLTILYQGNI 250 260 270 280 290 300 PGLEIESPQE EGSRWIGVEP IEGSLVVIMG DLSHIISNGQ FRSTMHRAVV NKTHHRVSAA 310 320 330 340 YFAGPPKNLQ IGPLTSDKNH PPIYRRLIWE EYLAAKATHF NKALTLFRC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations." Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P. Plant Biotechnol. J. 4:317-324(2006) [PubMed: 17147637] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL035527 Genomic DNA. Translation: CAB36813.1. AL161555 Genomic DNA. Translation: CAB81276.1. CP002687 Genomic DNA. Translation: AEE84490.1. DQ446858 mRNA. Translation: ABE66082.1.
IPI	IPI00532909.
PIR	T05844.
RefSeq	NP_193900.1. NM_118289.1.
UniGene	At.54461.
3D structure databases
ProteinModelPortal	Q9SVS8.
SMR	Q9SVS8. Positions 45-348.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT4G21690.1; AT4G21690.1; AT4G21690.
GeneID	828256.
KEGG	ath:AT4G21690.
NMPDR	fig|3702.1.peg.20013.
Organism-specific databases
GeneFarm	5169. 494.
TAIR	At4g21690.
Phylogenomic databases
GeneTree	EPGT00050000009701.
InParanoid	Q9SVS8.
OMA	RVPETHA.
PhylomeDB	Q9SVS8.
ProtClustDB	PLN02254.
Gene expression databases
ArrayExpress	Q9SVS8.
Genevestigator	Q9SVS8.
Family and domain databases
InterPro	IPR002283. Isopenicillin-N_synthase. IPR005123. Oxoglutarate/Fe-dep_oxygenase. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
PRINTS	PR00682. IPNSYNTHASE.
PROSITE	PS51471. FE2OG_OXY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3OX3_ARATH

Accession

Primary (citable) accession number: Q9SVS8
Secondary accession number(s): Q1PE60

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program