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Q9SVN5 (SYM_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable methionine--tRNA ligase
EC=6.1.1.10
Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS
Gene names
Ordered Locus Names:At4g13780
ORF Names:F18A5.170
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length797 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).

Subcellular location

Cytoplasm Probable.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Contains 1 tRNA-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 797797Probable methionine--tRNA ligase
PRO_0000139266

Regions

Domain635 – 738104tRNA-binding
Motif26 – 3611"HIGH" region
Motif348 – 3525"KMSKS" region

Sites

Binding site3511ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9SVN5 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: B0F523C2E0C1017F

FASTA79789,854
        10         20         30         40         50         60 
MEDDGKSSPK LPIPGKRNIL ITSALPYVNN VPHLGNIIGC VLSADVYARY CRLRGYNAIY 

        70         80         90        100        110        120 
ICGTDEYGTA TETKALEENC TPKEICDKYH AIHKEVYDWF GISFDKFGRT STPEQTEVCQ 

       130        140        150        160        170        180 
AIFNKLWDNK WLSENTMQQL YCDTCKKFLA DRLVEGSCPF EGCNYDSARG DQCEKCGKLL 

       190        200        210        220        230        240 
NPTELKDPKC KVCQNTPRIR DTDHLFIELP LLKDRLEAYI KKTSVTGSWS QNAIQTTNAW 

       250        260        270        280        290        300 
LRDGLRQRCI TRDLKWGVPV PHEKYKDKVF YVWFDAPIGY VSITSCYTSE WEKWWKNPEN 

       310        320        330        340        350        360 
VELYQFMGKD NVPFHTVMFP STQLGTEENW TLMKTISVTE YLNYEDGKFS KSKGVGVFGN 

       370        380        390        400        410        420 
DVKDTNIPVE VWRYYLLTNR PEVSDTSFSW TDLQAKLNGE LLSNLGNFVN RVLSFIAKPD 

       430        440        450        460        470        480 
NAGYGSVIPD AHDAESHSLT KSLAEKVEKF VAEYVEAMEK VKLKQGLKTA MLISSEGNYY 

       490        500        510        520        530        540 
LQASQFWKLY KEDKPLCAVV IRTAAGLVHL LAQLLEPFMP SFSCEVFKQL NLPPQFSLSD 

       550        560        570        580        590        600 
ERGEVLLASR PWDILPPSHR IGTPQPLFKE LENDEVARYR EKFAGSQSDR RARDEAANLA 

       610        620        630        640        650        660 
DQLNKTKLSD AKKQKASSKG GGKPKPQPAA DREITMARLD IRVGKIVKAE KHPKADALYV 

       670        680        690        700        710        720 
EEIDVGGGEI RTVVSGLVKY IPLEEMQNRM VCVLCNLKPA KMRDIVSQAM VLAASSSDGS 

       730        740        750        760        770        780 
KVELVEPPKT ANIGERVTFP GFEGEPDDVL NPKKKVWETL LVDLNTKENL VACYKDVPFT 

       790 
TSAGVCKVSS ISNGTIR

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. expand/collapse author list , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
Nature 402:769-777(1999) [PubMed: 10617198] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AL035528 Genomic DNA. Translation: CAB36842.1.
AL161537 Genomic DNA. Translation: CAB78420.1.
CP002687 Genomic DNA. Translation: AEE83324.1.
AY064009 mRNA. Translation: AAL36365.1.
AY091454 mRNA. Translation: AAM14393.1.
IPIIPI00527871.
PIRT05247.
RefSeqNP_193114.1. NM_117452.4.
UniGeneAt.27407.

3D structure databases

ProteinModelPortalQ9SVN5.
SMRQ9SVN5. Positions 17-571, 633-797.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ9SVN5.

Proteomic databases

PRIDEQ9SVN5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT4G13780.1; AT4G13780.1; AT4G13780.
GeneID827012.
GenomeReviewsGene locus AT4G13780 in contig CT486007_GR.
KEGGath:AT4G13780.
NMPDRfig|3702.1.peg.19071.

Organism-specific databases

GeneFarm2798.
TAIRAt4g13780.

Phylogenomic databases

eggNOGKOG1247.
GeneTreeEPGT00050000011635.
HOGENOMHBG721667.
InParanoidQ9SVN5.
OMAHEIAPFK.
PhylomeDBQ9SVN5.
ProtClustDBPLN02610.

Gene expression databases

ArrayExpressQ9SVN5.
GenevestigatorQ9SVN5.
GermOnlineAT4G13780. Arabidopsis thaliana.

Family and domain databases

InterProIPR015413. aa-tRNA-synt_I.
IPR001412. aa-tRNA-synth_I_CS.
IPR014758. Met-tRNA_synth.
IPR023458. Met-tRNA_synth_1.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KOK01874.
PfamPF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSPR01041. TRNASYNTHMET.
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMsTIGR00398. MetG. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYM_ARATH
AccessionPrimary (citable) accession number: Q9SVN5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: May 1, 2000
Last modified: January 25, 2012
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents