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Protein

Adenine nucleotide transporter BT1, chloroplastic/mitochondrial

Gene

BT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable mitochondrial adenylate carrier that catalyzes the transport of ATP, ADP and AMP, but not ADP-glucose. Recombinant BT1 shows a unidirectional mode of transport in intact E.coli cells. May function as a plastidial nucleotide uniport carrier required to export newly synthesized adenylates into the cytosol. May be involved in abiotic stress response.3 Publications

Miscellaneous

The growth retardation observed in plants silencing BT1 is circumvented by adenosine feeding.1 Publication

Enzyme regulationi

Inhibited by pyridoxal 5-phosphate but not mersalyl.1 Publication

Kineticsi

  1. KM=424 µM for ATP (for the recombinant protein in intact E.coli cells)1 Publication
  2. KM=390 µM for ADP (for the recombinant protein in intact E.coli cells)1 Publication
  3. KM=331 µM for AMP (for the recombinant protein in intact E.coli cells)1 Publication
  1. Vmax=1.02 nmol/h/mg enzyme toward ATP (for the recombinant protein in intact E.coli cells)1 Publication
  2. Vmax=2.65 nmol/h/mg enzyme toward ADP (for the recombinant protein in intact E.coli cells)1 Publication
  3. Vmax=8.72 mmol/h/mg enzyme toward AMP (for the recombinant protein in intact E.coli cells)1 Publication

GO - Biological processi

Keywordsi

Biological processStress response, Transport

Protein family/group databases

TCDBi2.A.29.11.4. the mitochondrial carrier (mc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenine nucleotide transporter BT1, chloroplastic/mitochondrial
Alternative name(s):
Protein BRITTLE 1 homolog
Short name:
AtBT1
Protein EMBRYO DEFECTIVE 104
Protein EMBRYO DEFECTIVE 42
Protein SODIUM HYPERSENSITIVE 1
Gene namesi
Name:BT1
Synonyms:EMB104, EMB42, SHS1
Ordered Locus Names:At4g32400
ORF Names:F8B4.100
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G32400.
TAIRilocus:2127851. AT4G32400.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei113 – 133Helical; Name=1Sequence analysisAdd BLAST21
Transmembranei168 – 188Helical; Name=2Sequence analysisAdd BLAST21
Transmembranei204 – 224Helical; Name=3Sequence analysisAdd BLAST21
Transmembranei263 – 283Helical; Name=4Sequence analysisAdd BLAST21
Transmembranei302 – 322Helical; Name=5Sequence analysisAdd BLAST21
Transmembranei359 – 379Helical; Name=6Sequence analysisAdd BLAST21

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • chloroplast inner membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: GO_Central
  • mitochondrial inner membrane Source: GO_Central
  • mitochondrion Source: TAIR
  • plastid Source: TAIR

Keywords - Cellular componenti

Chloroplast, Membrane, Mitochondrion, Mitochondrion inner membrane, Plastid, Plastid inner membrane

Pathology & Biotechi

Disruption phenotypei

Impaired growth, flower and silique development, and production of shrunk and sterile seeds.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000420802? – 392Adenine nucleotide transporter BT1, chloroplastic/mitochondrial
Transit peptidei1 – ?Chloroplast and mitochondrionSequence analysis

Proteomic databases

PaxDbiQ9SUV1.

PTM databases

iPTMnetiQ9SUV1.

Expressioni

Tissue specificityi

Expressed in root tips, the central cylinder of young roots, and maturating and germinating pollen.1 Publication

Gene expression databases

ExpressionAtlasiQ9SUV1. baseline and differential.
GenevisibleiQ9SUV1. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G32400.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SUV1.
SMRiQ9SUV1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati108 – 191Solcar 1Add BLAST84
Repeati202 – 286Solcar 2Add BLAST85
Repeati296 – 384Solcar 3Add BLAST89

Sequence similaritiesi

Keywords - Domaini

Repeat, Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0752. Eukaryota.
ENOG410ZRF1. LUCA.
HOGENOMiHOG000165726.
InParanoidiQ9SUV1.
OMAiYMQVYSS.
OrthoDBiEOG09360G3P.
PhylomeDBiQ9SUV1.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiView protein in InterPro
IPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
PfamiView protein in Pfam
PF00153. Mito_carr. 3 hits.
PRINTSiPR00926. MITOCARRIER.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiView protein in PROSITE
PS50920. SOLCAR. 3 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SUV1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKTGIQLFD DSRNGFFSVS DLGFDSSLNS SNYHPIGGLF ASVNQTNPFA
60 70 80 90 100
SLSSSDLSNR GNNSFSTQLN DLYTKYMPGK EEEEEVVNGE KRKRKKKGGL
110 120 130 140 150
TLKIKIANPS LRRLLSGAVA GAVSRTVVAP LETIRTHLMV GSGGNSSTEV
160 170 180 190 200
FSDIMKHEGW TGLFRGNLVN VIRVAPARAV ELFVFETVNK KLSPPHGQES
210 220 230 240 250
KIPIPASLLA GACAGVSQTL LTYPLELVKT RLTIQRGVYK GIFDAFLKII
260 270 280 290 300
REEGPTELYR GLAPSLIGVV PYAATNYFAY DSLRKAYRSF SKQEKIGNIE
310 320 330 340 350
TLLIGSLAGA LSSTATFPLE VARKHMQVGA VSGRVVYKNM LHALVTILEH
360 370 380 390
EGILGWYKGL GPSCLKLVPA AGISFMCYEA CKKILIENNQ EA
Length:392
Mass (Da):42,571
Last modified:May 1, 2000 - v1
Checksum:i556269D8C67640C2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL034567 Genomic DNA. Translation: CAA22567.1.
AL161581 Genomic DNA. Translation: CAB79957.1.
CP002687 Genomic DNA. Translation: AEE86052.1.
AF372944 mRNA. Translation: AAK50084.1.
AY074831 mRNA. Translation: AAL69529.1.
AY084938 mRNA. Translation: AAM61499.1.
PIRiT05350.
RefSeqiNP_194966.1. NM_119392.4.
UniGeneiAt.2510.

Genome annotation databases

EnsemblPlantsiAT4G32400.1; AT4G32400.1; AT4G32400.
GeneIDi829375.
GrameneiAT4G32400.1; AT4G32400.1; AT4G32400.
KEGGiath:AT4G32400.

Similar proteinsi

Entry informationi

Entry nameiBRT1_ARATH
AccessioniPrimary (citable) accession number: Q9SUV1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: May 1, 2000
Last modified: June 7, 2017
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families