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Protein

Adenine nucleotide transporter BT1, chloroplastic/mitochondrial

Gene

BT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable mitochondrial adenylate carrier that catalyzes the transport of ATP, ADP and AMP, but not ADP-glucose. Recombinant BT1 shows a unidirectional mode of transport in intact E.coli cells. May function as a plastidial nucleotide uniport carrier required to export newly synthesized adenylates into the cytosol. May be involved in abiotic stress response.3 Publications

Miscellaneous

The growth retardation observed in plants silencing BT1 is circumvented by adenosine feeding.1 Publication

Enzyme regulationi

Inhibited by pyridoxal 5-phosphate but not mersalyl.1 Publication

Kineticsi

  1. KM=424 µM for ATP (for the recombinant protein in intact E.coli cells)1 Publication
  2. KM=390 µM for ADP (for the recombinant protein in intact E.coli cells)1 Publication
  3. KM=331 µM for AMP (for the recombinant protein in intact E.coli cells)1 Publication
  1. Vmax=1.02 nmol/h/mg enzyme toward ATP (for the recombinant protein in intact E.coli cells)1 Publication
  2. Vmax=2.65 nmol/h/mg enzyme toward ADP (for the recombinant protein in intact E.coli cells)1 Publication
  3. Vmax=8.72 mmol/h/mg enzyme toward AMP (for the recombinant protein in intact E.coli cells)1 Publication

GO - Biological processi

Keywordsi

Biological processStress response, Transport

Protein family/group databases

TCDBi2.A.29.11.4 the mitochondrial carrier (mc) family

Names & Taxonomyi

Protein namesi
Recommended name:
Adenine nucleotide transporter BT1, chloroplastic/mitochondrial
Alternative name(s):
Protein BRITTLE 1 homolog
Short name:
AtBT1
Protein EMBRYO DEFECTIVE 104
Protein EMBRYO DEFECTIVE 42
Protein SODIUM HYPERSENSITIVE 1
Gene namesi
Name:BT1
Synonyms:EMB104, EMB42, SHS1
Ordered Locus Names:At4g32400
ORF Names:F8B4.100
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G32400
TAIRilocus:2127851 AT4G32400

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei113 – 133Helical; Name=1Sequence analysisAdd BLAST21
Transmembranei168 – 188Helical; Name=2Sequence analysisAdd BLAST21
Transmembranei204 – 224Helical; Name=3Sequence analysisAdd BLAST21
Transmembranei263 – 283Helical; Name=4Sequence analysisAdd BLAST21
Transmembranei302 – 322Helical; Name=5Sequence analysisAdd BLAST21
Transmembranei359 – 379Helical; Name=6Sequence analysisAdd BLAST21

Keywords - Cellular componenti

Chloroplast, Membrane, Mitochondrion, Mitochondrion inner membrane, Plastid, Plastid inner membrane

Pathology & Biotechi

Disruption phenotypei

Impaired growth, flower and silique development, and production of shrunk and sterile seeds.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000420802? – 392Adenine nucleotide transporter BT1, chloroplastic/mitochondrial
Transit peptidei1 – ?Chloroplast and mitochondrionSequence analysis

Proteomic databases

PaxDbiQ9SUV1

PTM databases

iPTMnetiQ9SUV1

Expressioni

Tissue specificityi

Expressed in root tips, the central cylinder of young roots, and maturating and germinating pollen.1 Publication

Gene expression databases

ExpressionAtlasiQ9SUV1 baseline and differential
GenevisibleiQ9SUV1 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G32400.1

Structurei

3D structure databases

ProteinModelPortaliQ9SUV1
SMRiQ9SUV1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati108 – 191Solcar 1Add BLAST84
Repeati202 – 286Solcar 2Add BLAST85
Repeati296 – 384Solcar 3Add BLAST89

Sequence similaritiesi

Keywords - Domaini

Repeat, Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0752 Eukaryota
ENOG410ZRF1 LUCA
HOGENOMiHOG000165726
InParanoidiQ9SUV1
OMAiYMQVYSS
OrthoDBiEOG09360G3P
PhylomeDBiQ9SUV1

Family and domain databases

Gene3Di1.50.40.10, 1 hit
InterProiView protein in InterPro
IPR002067 Mit_carrier
IPR018108 Mitochondrial_sb/sol_carrier
IPR023395 Mt_carrier_dom_sf
PfamiView protein in Pfam
PF00153 Mito_carr, 3 hits
PRINTSiPR00926 MITOCARRIER
SUPFAMiSSF103506 SSF103506, 1 hit
PROSITEiView protein in PROSITE
PS50920 SOLCAR, 3 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SUV1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKTGIQLFD DSRNGFFSVS DLGFDSSLNS SNYHPIGGLF ASVNQTNPFA
60 70 80 90 100
SLSSSDLSNR GNNSFSTQLN DLYTKYMPGK EEEEEVVNGE KRKRKKKGGL
110 120 130 140 150
TLKIKIANPS LRRLLSGAVA GAVSRTVVAP LETIRTHLMV GSGGNSSTEV
160 170 180 190 200
FSDIMKHEGW TGLFRGNLVN VIRVAPARAV ELFVFETVNK KLSPPHGQES
210 220 230 240 250
KIPIPASLLA GACAGVSQTL LTYPLELVKT RLTIQRGVYK GIFDAFLKII
260 270 280 290 300
REEGPTELYR GLAPSLIGVV PYAATNYFAY DSLRKAYRSF SKQEKIGNIE
310 320 330 340 350
TLLIGSLAGA LSSTATFPLE VARKHMQVGA VSGRVVYKNM LHALVTILEH
360 370 380 390
EGILGWYKGL GPSCLKLVPA AGISFMCYEA CKKILIENNQ EA
Length:392
Mass (Da):42,571
Last modified:May 1, 2000 - v1
Checksum:i556269D8C67640C2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL034567 Genomic DNA Translation: CAA22567.1
AL161581 Genomic DNA Translation: CAB79957.1
CP002687 Genomic DNA Translation: AEE86052.1
AF372944 mRNA Translation: AAK50084.1
AY074831 mRNA Translation: AAL69529.1
AY084938 mRNA Translation: AAM61499.1
PIRiT05350
RefSeqiNP_194966.1, NM_119392.4
UniGeneiAt.2510

Genome annotation databases

EnsemblPlantsiAT4G32400.1; AT4G32400.1; AT4G32400
GeneIDi829375
GrameneiAT4G32400.1; AT4G32400.1; AT4G32400
KEGGiath:AT4G32400

Similar proteinsi

Entry informationi

Entry nameiBRT1_ARATH
AccessioniPrimary (citable) accession number: Q9SUV1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: May 1, 2000
Last modified: April 25, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health