Q9SUP6 (WRK53_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable WRKY transcription factor 53

Alternative name(s):
WRKY DNA-binding protein 53

Gene names

Name:	WRKY53
Ordered Locus Names:	At4g23810
ORF Names:	F9D16.280

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. May regulate the early events of leaf senescence. Negatively regulates the expression of ESR/ESP. Ref.9
Subunit structure	Interacts with ESR/ESP and UPL5. Ref.8 Ref.9
Subcellular location	Nucleus Ref.8.
Induction	By salicylic acid and strongly during leaf senescence. Down-regulated by jasmonate. Ref.1 Ref.7 Ref.8
Post-translational modification	Ubiquitinated by UPL5. Ubiquitination leads to its subsequent degradation, thus controlling the timing of leaf senescence. Ref.9
Disruption phenotype	Retarded leaf senescence, but no effects on pathogen resistance. Ref.8
Sequence similarities	Belongs to the WRKY group III family. Contains 1 WRKY DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
PTM	Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	defense response to bacterium, incompatible interaction Inferred from mutant phenotype. Source: TAIR leaf senescence Inferred from mutant phenotype Ref.9. Source: TAIR positive regulation of transcription, DNA-dependent Inferred from direct assay. Source: TAIR regulation of defense response Inferred from mutant phenotype. Source: TAIR response to chitin Inferred from expression pattern. Source: TAIR transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from direct assay. Source: TAIR nucleus Inferred from direct assay Ref.8Ref.9. Source: TAIR
Molecular function	protein binding Inferred from physical interaction Ref.9. Source: UniProtKB sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from mutant phenotype. Source: TAIR
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CAM1	P25854	2	EBI-1235980,EBI-1235664
CAM6	Q03509	2	EBI-1235980,EBI-1236097
CML9	Q9S744	2	EBI-1235980,EBI-1236048
ESP	Q8RY71	6	EBI-1235980,EBI-1997188
MEKK1	Q39008	5	EBI-1235980,EBI-994439

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	Probable WRKY transcription factor 53		PRO_0000133694
Regions
	DNA binding	152 – 220	69	WRKY

Sequences

	Sequence		Length	Mass (Da)
	Q9SUP6 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 017A5316B2C27578	FASTA	324	36,273
	10 20 30 40 50 60 MEGRDMLSWE QKTLLSELIN GFDAAKKLQA RLREAPSPSS SFSSPATAVA ETNEILVKQI 70 80 90 100 110 120 VSSYERSLLL LNWSSSPSVQ LIPTPVTVVP VANPGSVPES PASINGSPRS EEFADGGGSS 130 140 150 160 170 180 ESHHRQDYIF NSKKRKMLPK WSEKVRISPE RGLEGPQDDV FSWRKYGQKD ILGAKFPRSY 190 200 210 220 230 240 YRCTHRSTQN CWATKQVQRS DGDATVFEVT YRGTHTCSQA ITRTPPLASP EKRQDTRVKP 250 260 270 280 290 300 AITQKPKDIL ESLKSNLTVR TDGLDDGKDV FSFPDTPPFY NYGTINGEFG HVESSPIFDV 310 320 VDWFNPTVEI DTTFPAFLHE SIYY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evidence for an important role of WRKY DNA binding proteins in the regulation of NPR1 gene expression." Yu D., Chen C., Chen Z. Plant Cell 13:1527-1540(2001) [PubMed: 11449049] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
[2]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Arabidopsis ORF clones." De Los Reyes C., Quan R., Chen H., Bautista V.R., Kim C.J., Ecker J.R. Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]	"Identification of a transcription factor specifically expressed at the onset of leaf senescence." Hinderhofer K., Zentgraf U. Planta 213:469-473(2001) [PubMed: 11506370] [Abstract] Cited for: INDUCTION. Strain: cv. Columbia.
[8]	"The antagonist function of Arabidopsis WRKY53 and ESR/ESP in leaf senescence is modulated by the jasmonic and salicylic acid equilibrium." Miao Y., Zentgraf U. Plant Cell 19:819-830(2007) [PubMed: 17369373] [Abstract] Cited for: SUBCELLULAR LOCATION, INDUCTION, INTERACTION WITH ESP, DISRUPTION PHENOTYPE.
[9]	"A HECT E3 ubiquitin ligase negatively regulates Arabidopsis leaf senescence through degradation of the transcription factor WRKY53." Miao Y., Zentgraf U. Plant J. 63:179-188(2010) [PubMed: 20409006] [Abstract] Cited for: FUNCTION, INTERACTION WITH UPL5, UBIQUITINATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF272748 mRNA. Translation: AAK28442.1. AL035394 Genomic DNA. Translation: CAA23047.1. AL161560 Genomic DNA. Translation: CAB81299.1. CP002687 Genomic DNA. Translation: AEE84809.1. AF370614 mRNA. Translation: AAK43933.1. BT033113 mRNA. Translation: ACF20468.1. AK227938 mRNA. Translation: BAE99906.1.
IPI	IPI00538078.
PIR	T05613.
RefSeq	NP_194112.1. NM_118512.3.
UniGene	At.2572. At.71059.
3D structure databases
ProteinModelPortal	Q9SUP6.
SMR	Q9SUP6. Positions 155-220.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9SUP6. 10 interactions.
STRING	Q9SUP6.
Proteomic databases
PRIDE	Q9SUP6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT4G23810.1; AT4G23810.1; AT4G23810.
GeneID	828481.
GenomeReviews	Gene locus AT4G23810 in contig CT486007_GR.
KEGG	ath:AT4G23810.
NMPDR	fig|3702.1.peg.20260.
Organism-specific databases
TAIR	At4g23810.
Phylogenomic databases
GeneTree	EPGT00050000007685.
HOGENOM	HBG747767.
InParanoid	Q9SUP6.
OMA	SPASING.
PhylomeDB	Q9SUP6.
ProtClustDB	CLSN2686002.
Gene expression databases
ArrayExpress	Q9SUP6.
Genevestigator	Q9SUP6.
GermOnline	AT4G23810. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR003657. WRKY_DNA-bd. [Graphical view]
Gene3D	G3DSA:2.20.25.80. WRKY_DNA-bd. 1 hit.
Pfam	PF03106. WRKY. 1 hit. [Graphical view]
SMART	SM00774. WRKY. 1 hit. [Graphical view]
SUPFAM	SSF118290. SSF118290. 1 hit.
PROSITE	PS50811. WRKY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

WRK53_ARATH

Accession

Primary (citable) accession number: Q9SUP6
Secondary accession number(s): Q0WSJ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 11, 2003
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program