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Protein

NADPH--cytochrome P450 reductase 2

Gene

ATR2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Reduces a variety of substrates in vitro, such as cytochrome c, feericyanide and dichloroindophenol.UniRule annotation5 Publications

Catalytic activityi

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • FADUniRule annotation1 PublicationNote: Binds 1 FAD per monomer.UniRule annotation
  • FMNUniRule annotation1 PublicationNote: Binds 1 FMN per monomer.UniRule annotation

Kineticsi

  1. KM=23 µM for NADPH (at pH 7.7 and 28 degrees Celsius)4 Publications
  2. KM=2.0 µM for NADPH (at pH 7.25 and 25 degrees Celsius)4 Publications
  3. KM=15 µM for cytochrome c (at pH 7.7 and 30 degrees Celsius)4 Publications
  4. KM=16 µM for cytochrome c (at pH 7.0 and 25 degrees Celsius)4 Publications
  5. KM=22.5 µM for cytochrome c (at pH 7.7 and 28 degrees Celsius)4 Publications

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei239FMNUniRule annotation1
    Binding sitei330NADPUniRule annotation1
    Binding sitei570NADPUniRule annotation1
    Binding sitei673NADPUniRule annotation1
    Binding sitei711FADUniRule annotation1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi111 – 116FMNUniRule annotation6
    Nucleotide bindingi166 – 169FMNUniRule annotation4
    Nucleotide bindingi204 – 213FMNUniRule annotation10
    Nucleotide bindingi489 – 492FADUniRule annotation4
    Nucleotide bindingi507 – 509FADUniRule annotation3
    Nucleotide bindingi523 – 526FADUniRule annotation4
    Nucleotide bindingi631 – 632NADPUniRule annotation2
    Nucleotide bindingi637 – 641NADPUniRule annotation5

    GO - Molecular functioni

    • FMN binding Source: InterPro
    • NADPH-hemoprotein reductase activity Source: TAIR

    GO - Biological processi

    • phenylpropanoid metabolic process Source: TAIR

    Keywordsi

    Molecular functionOxidoreductase
    Biological processPhenylpropanoid metabolism
    LigandFAD, Flavoprotein, FMN, NADP

    Enzyme and pathway databases

    ReactomeiR-ATH-211897. Cytochrome P450 - arranged by substrate type.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADPH--cytochrome P450 reductase 2UniRule annotation (EC:1.6.2.4UniRule annotation)
    Short name:
    CPR 2UniRule annotation
    Short name:
    P450R 2UniRule annotation
    Gene namesi
    Name:ATR2
    Synonyms:AR2
    Ordered Locus Names:At4g30210
    ORF Names:F9N11.60
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 4

    Organism-specific databases

    TAIRilocus:2128951. AT4G30210.

    Subcellular locationi

    • Endoplasmic reticulum membrane UniRule annotation; Single-pass membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 51LumenalUniRule annotationAdd BLAST51
    Transmembranei52 – 72HelicalUniRule annotationAdd BLAST21
    Topological domaini73 – 711CytoplasmicUniRule annotationAdd BLAST639

    GO - Cellular componenti

    • chloroplast Source: TAIR
    • endoplasmic reticulum Source: TAIR
    • endoplasmic reticulum membrane Source: UniProtKB-SubCell
    • integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004168401 – 711NADPH--cytochrome P450 reductase 2Add BLAST711

    Proteomic databases

    PaxDbiQ9SUM3.

    PTM databases

    iPTMnetiQ9SUM3.

    Expressioni

    Tissue specificityi

    Expressed in roots, leaves, stems, flowers and siliques.1 Publication

    Inductioni

    By wounding and transition from dark to light.1 Publication

    Gene expression databases

    ExpressionAtlasiQ9SUM3. baseline and differential.
    GenevisibleiQ9SUM3. AT.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT4G30210.1.

    Structurei

    Secondary structure

    1711
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi104 – 110Combined sources7
    Beta strandi112 – 114Combined sources3
    Helixi115 – 130Combined sources16
    Turni131 – 133Combined sources3
    Beta strandi134 – 140Combined sources7
    Helixi141 – 144Combined sources4
    Helixi148 – 155Combined sources8
    Beta strandi159 – 168Combined sources10
    Helixi169 – 171Combined sources3
    Turni175 – 177Combined sources3
    Helixi178 – 185Combined sources8
    Beta strandi198 – 205Combined sources8
    Beta strandi209 – 211Combined sources3
    Helixi214 – 225Combined sources12
    Beta strandi229 – 232Combined sources4
    Beta strandi235 – 238Combined sources4
    Helixi243 – 259Combined sources17
    Beta strandi309 – 311Combined sources3
    Beta strandi313 – 322Combined sources10
    Beta strandi332 – 338Combined sources7
    Beta strandi351 – 354Combined sources4
    Helixi360 – 370Combined sources11
    Helixi403 – 409Combined sources7
    Helixi419 – 426Combined sources8
    Beta strandi429 – 431Combined sources3
    Helixi432 – 442Combined sources11
    Helixi444 – 446Combined sources3
    Helixi447 – 453Combined sources7
    Turni454 – 458Combined sources5
    Helixi461 – 467Combined sources7
    Helixi475 – 478Combined sources4
    Turni479 – 482Combined sources4
    Beta strandi489 – 492Combined sources4
    Turni497 – 499Combined sources3
    Beta strandi501 – 509Combined sources9
    Beta strandi512 – 514Combined sources3
    Beta strandi520 – 522Combined sources3
    Helixi524 – 531Combined sources8
    Beta strandi543 – 549Combined sources7
    Beta strandi563 – 566Combined sources4
    Helixi569 – 572Combined sources4
    Helixi573 – 586Combined sources14
    Turni587 – 589Combined sources3
    Beta strandi595 – 602Combined sources8
    Turni604 – 606Combined sources3
    Helixi611 – 619Combined sources9
    Beta strandi624 – 635Combined sources12
    Helixi640 – 646Combined sources7
    Helixi648 – 656Combined sources9
    Beta strandi660 – 665Combined sources6
    Turni667 – 669Combined sources3
    Helixi670 – 686Combined sources17
    Helixi690 – 703Combined sources14
    Beta strandi705 – 710Combined sources6

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    5GXUX-ray2.30A/B73-711[»]
    ProteinModelPortaliQ9SUM3.
    SMRiQ9SUM3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini105 – 255Flavodoxin-likeUniRule annotationAdd BLAST151
    Domaini310 – 556FAD-binding FR-typeUniRule annotationAdd BLAST247

    Sequence similaritiesi

    Belongs to the NADPH--cytochrome P450 reductase family.UniRule annotation
    In the N-terminal section; belongs to the flavodoxin family.UniRule annotation
    In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.UniRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1158. Eukaryota.
    COG0369. LUCA.
    HOGENOMiHOG000282027.
    InParanoidiQ9SUM3.
    KOiK00327.
    OMAiVWYENDP.
    PhylomeDBiQ9SUM3.

    Family and domain databases

    Gene3Di1.20.990.10. 1 hit.
    3.40.50.360. 1 hit.
    HAMAPiMF_03212. NCPR. 1 hit.
    InterProiView protein in InterPro
    IPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin-like.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like_dom.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR023208. P450R.
    IPR017938. Riboflavin_synthase-like_b-brl.
    PfamiView protein in Pfam
    PF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    PIRSFiPIRSF000208. P450R. 1 hit.
    PRINTSiPR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMiSSF52218. SSF52218. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEiView protein in PROSITE
    PS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q9SUM3-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MSSSSSSSTS MIDLMAAIIK GEPVIVSDPA NASAYESVAA ELSSMLIENR
    60 70 80 90 100
    QFAMIVTTSI AVLIGCIVML VWRRSGSGNS KRVEPLKPLV IKPREEEIDD
    110 120 130 140 150
    GRKKVTIFFG TQTGTAEGFA KALGEEAKAR YEKTRFKIVD LDDYAADDDE
    160 170 180 190 200
    YEEKLKKEDV AFFFLATYGD GEPTDNAARF YKWFTEGNDR GEWLKNLKYG
    210 220 230 240 250
    VFGLGNRQYE HFNKVAKVVD DILVEQGAQR LVQVGLGDDD QCIEDDFTAW
    260 270 280 290 300
    REALWPELDT ILREEGDTAV ATPYTAAVLE YRVSIHDSED AKFNDINMAN
    310 320 330 340 350
    GNGYTVFDAQ HPYKANVAVK RELHTPESDR SCIHLEFDIA GSGLTYETGD
    360 370 380 390 400
    HVGVLCDNLS ETVDEALRLL DMSPDTYFSL HAEKEDGTPI SSSLPPPFPP
    410 420 430 440 450
    CNLRTALTRY ACLLSSPKKS ALVALAAHAS DPTEAERLKH LASPAGKDEY
    460 470 480 490 500
    SKWVVESQRS LLEVMAEFPS AKPPLGVFFA GVAPRLQPRF YSISSSPKIA
    510 520 530 540 550
    ETRIHVTCAL VYEKMPTGRI HKGVCSTWMK NAVPYEKSEN CSSAPIFVRQ
    560 570 580 590 600
    SNFKLPSDSK VPIIMIGPGT GLAPFRGFLQ ERLALVESGV ELGPSVLFFG
    610 620 630 640 650
    CRNRRMDFIY EEELQRFVES GALAELSVAF SREGPTKEYV QHKMMDKASD
    660 670 680 690 700
    IWNMISQGAY LYVCGDAKGM ARDVHRSLHT IAQEQGSMDS TKAEGFVKNL
    710
    QTSGRYLRDV W
    Length:711
    Mass (Da):78,927
    Last modified:May 1, 2000 - v1
    Checksum:iF0503108EB7F508F
    GO
    Isoform 2 (identifier: Q9SUM3-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         447-447: K → KV

    Note: May be due to a competing donor splice site. No experimental confirmation available.
    Show »
    Length:712
    Mass (Da):79,026
    Checksum:i75656CFE0CC4DB63
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti297 – 298NM → TL in CAA46815 (PubMed:9235908).Curated2
    Sequence conflicti346 – 348YET → MKL in CAA46815 (PubMed:9235908).Curated3
    Sequence conflicti540 – 544NCSSA → KLFLGR in CAA46815 (PubMed:9235908).Curated5

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_042906447K → KV in isoform 2. Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X66017 mRNA. Translation: CAA46815.1.
    AL109796 Genomic DNA. Translation: CAB52465.1.
    AL161576 Genomic DNA. Translation: CAB81014.1.
    CP002687 Genomic DNA. Translation: AEE85737.1.
    CP002687 Genomic DNA. Translation: AEE85738.1.
    AF325101 mRNA. Translation: AAK17169.1.
    PIRiS21531.
    T14081.
    RefSeqiNP_194750.1. NM_119167.4. [Q9SUM3-1]
    NP_849472.2. NM_179141.2. [Q9SUM3-2]
    UniGeneiAt.145.

    Genome annotation databases

    EnsemblPlantsiAT4G30210.2; AT4G30210.2; AT4G30210. [Q9SUM3-1]
    GeneIDi829144.
    GrameneiAT4G30210.2; AT4G30210.2; AT4G30210.
    KEGGiath:AT4G30210.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X66017 mRNA. Translation: CAA46815.1.
    AL109796 Genomic DNA. Translation: CAB52465.1.
    AL161576 Genomic DNA. Translation: CAB81014.1.
    CP002687 Genomic DNA. Translation: AEE85737.1.
    CP002687 Genomic DNA. Translation: AEE85738.1.
    AF325101 mRNA. Translation: AAK17169.1.
    PIRiS21531.
    T14081.
    RefSeqiNP_194750.1. NM_119167.4. [Q9SUM3-1]
    NP_849472.2. NM_179141.2. [Q9SUM3-2]
    UniGeneiAt.145.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    5GXUX-ray2.30A/B73-711[»]
    ProteinModelPortaliQ9SUM3.
    SMRiQ9SUM3.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi3702.AT4G30210.1.

    PTM databases

    iPTMnetiQ9SUM3.

    Proteomic databases

    PaxDbiQ9SUM3.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT4G30210.2; AT4G30210.2; AT4G30210. [Q9SUM3-1]
    GeneIDi829144.
    GrameneiAT4G30210.2; AT4G30210.2; AT4G30210.
    KEGGiath:AT4G30210.

    Organism-specific databases

    AraportiAT4G30210.
    TAIRilocus:2128951. AT4G30210.

    Phylogenomic databases

    eggNOGiKOG1158. Eukaryota.
    COG0369. LUCA.
    HOGENOMiHOG000282027.
    InParanoidiQ9SUM3.
    KOiK00327.
    OMAiVWYENDP.
    PhylomeDBiQ9SUM3.

    Enzyme and pathway databases

    ReactomeiR-ATH-211897. Cytochrome P450 - arranged by substrate type.

    Miscellaneous databases

    PROiPR:Q9SUM3.

    Gene expression databases

    ExpressionAtlasiQ9SUM3. baseline and differential.
    GenevisibleiQ9SUM3. AT.

    Family and domain databases

    Gene3Di1.20.990.10. 1 hit.
    3.40.50.360. 1 hit.
    HAMAPiMF_03212. NCPR. 1 hit.
    InterProiView protein in InterPro
    IPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin-like.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like_dom.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR023208. P450R.
    IPR017938. Riboflavin_synthase-like_b-brl.
    PfamiView protein in Pfam
    PF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    PIRSFiPIRSF000208. P450R. 1 hit.
    PRINTSiPR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMiSSF52218. SSF52218. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEiView protein in PROSITE
    PS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiNCPR2_ARATH
    AccessioniPrimary (citable) accession number: Q9SUM3
    Secondary accession number(s): F4JPK2, Q39036
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 18, 2012
    Last sequence update: May 1, 2000
    Last modified: April 12, 2017
    This is version 115 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.