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Protein

Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial

Gene

At4g20930

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH.

Pathwayi: L-valine degradation

This protein is involved in the pathway L-valine degradation, which is part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the pathway L-valine degradation and in Amino-acid degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei134 – 1341NAD; via amide nitrogenBy similarity
Active sitei219 – 2191By similarity
Binding sitei294 – 2941NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi38 – 6730NADBy similarityAdd
BLAST
Nucleotide bindingi101 – 1022NADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Branched-chain amino acid catabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciARA:AT4G20930-MONOMER.
ReactomeiR-ATH-70895. Branched-chain amino acid catabolism.
UniPathwayiUPA00362.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial (EC:1.1.1.31)
Short name:
HIBADH
Gene namesi
Ordered Locus Names:At4g20930
ORF Names:T13K14.90
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G20930.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3434MitochondrionBy similarityAdd
BLAST
Chaini35 – 347313Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrialPRO_0000007162Add
BLAST

Proteomic databases

PaxDbiQ9SUC0.
PRIDEiQ9SUC0.

PTM databases

iPTMnetiQ9SUC0.

Expressioni

Gene expression databases

GenevisibleiQ9SUC0. AT.

Interactioni

Protein-protein interaction databases

BioGridi13132. 1 interaction.
STRINGi3702.AT4G20930.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SUC0.
SMRiQ9SUC0. Positions 40-338.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0409. Eukaryota.
COG2084. LUCA.
HOGENOMiHOG000219610.
InParanoidiQ9SUC0.
KOiK00020.
OMAiAVSESMC.
OrthoDBiEOG09360H9O.
PhylomeDBiQ9SUC0.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR002204. 3-OH-isobutyrate_DH-rel_CS.
IPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR006115. 6PGDH_NADP-bd.
IPR011548. HIBADH.
IPR015815. HIBADH-related.
IPR016040. NAD(P)-bd_dom.
IPR029154. NADP-bd.
[Graphical view]
PfamiPF14833. NAD_binding_11. 1 hit.
PF03446. NAD_binding_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000103. HIBADH. 1 hit.
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01692. HIBADH. 1 hit.
PROSITEiPS00895. 3_HYDROXYISOBUT_DH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SUC0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIRRAQTLL CLSKFKTNFV SGSLHRFSSS SQNSNQFQNV GFIGLGNMGF
60 70 80 90 100
RMVNNLIRAG YKVTVHDINR DVMKMFTEMG VSSRETPYEV AQDSEVVITM
110 120 130 140 150
LPSSSHVMDV YTGTNGLLLG ENDIRPALFI DSSTIDPQTT RKISLAVSNC
160 170 180 190 200
NLKEKRDNWE KPVMLDAPVS GGVLAAEAGT LTFMVGGPED AYLAARPILQ
210 220 230 240 250
SMGRTSIYCG GSGNGSAAKI CNNLAMAVSM LGTSEALALG QSLGISASTL
260 270 280 290 300
TEVLNTSSGR CWSSDAYNPV PGVMKGVPSS RDYNGGFASK LMAKDLNLAA
310 320 330 340
ASAEEVGHKS PLISKAQEIY KKMCEEGHET KDFSCVFRHF YNGKDEV
Length:347
Mass (Da):37,365
Last modified:April 30, 2003 - v3
Checksum:i6ED2E87CC3DE191C
GO

Sequence cautioni

The sequence CAB45888 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAB79093 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti21 – 211S → F in AAM63893 (Ref. 5) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL080282 Genomic DNA. Translation: CAB45888.1. Sequence problems.
AL161554 Genomic DNA. Translation: CAB79093.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE84377.1.
BT025657 mRNA. Translation: ABF74718.1.
AK228571 mRNA. Translation: BAF00489.1.
AY086845 mRNA. Translation: AAM63893.1.
RefSeqiNP_567617.1. NM_118211.4.
UniGeneiAt.32684.

Genome annotation databases

EnsemblPlantsiAT4G20930.1; AT4G20930.1; AT4G20930.
GeneIDi827841.
GrameneiAT4G20930.1; AT4G20930.1; AT4G20930.
KEGGiath:AT4G20930.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL080282 Genomic DNA. Translation: CAB45888.1. Sequence problems.
AL161554 Genomic DNA. Translation: CAB79093.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE84377.1.
BT025657 mRNA. Translation: ABF74718.1.
AK228571 mRNA. Translation: BAF00489.1.
AY086845 mRNA. Translation: AAM63893.1.
RefSeqiNP_567617.1. NM_118211.4.
UniGeneiAt.32684.

3D structure databases

ProteinModelPortaliQ9SUC0.
SMRiQ9SUC0. Positions 40-338.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi13132. 1 interaction.
STRINGi3702.AT4G20930.1.

PTM databases

iPTMnetiQ9SUC0.

Proteomic databases

PaxDbiQ9SUC0.
PRIDEiQ9SUC0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G20930.1; AT4G20930.1; AT4G20930.
GeneIDi827841.
GrameneiAT4G20930.1; AT4G20930.1; AT4G20930.
KEGGiath:AT4G20930.

Organism-specific databases

TAIRiAT4G20930.

Phylogenomic databases

eggNOGiKOG0409. Eukaryota.
COG2084. LUCA.
HOGENOMiHOG000219610.
InParanoidiQ9SUC0.
KOiK00020.
OMAiAVSESMC.
OrthoDBiEOG09360H9O.
PhylomeDBiQ9SUC0.

Enzyme and pathway databases

UniPathwayiUPA00362.
BioCyciARA:AT4G20930-MONOMER.
ReactomeiR-ATH-70895. Branched-chain amino acid catabolism.

Miscellaneous databases

PROiQ9SUC0.

Gene expression databases

GenevisibleiQ9SUC0. AT.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR002204. 3-OH-isobutyrate_DH-rel_CS.
IPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR006115. 6PGDH_NADP-bd.
IPR011548. HIBADH.
IPR015815. HIBADH-related.
IPR016040. NAD(P)-bd_dom.
IPR029154. NADP-bd.
[Graphical view]
PfamiPF14833. NAD_binding_11. 1 hit.
PF03446. NAD_binding_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000103. HIBADH. 1 hit.
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01692. HIBADH. 1 hit.
PROSITEiPS00895. 3_HYDROXYISOBUT_DH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry namei3HIDH_ARATH
AccessioniPrimary (citable) accession number: Q9SUC0
Secondary accession number(s): Q1ECP8, Q8LC25
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: April 30, 2003
Last modified: September 7, 2016
This is version 123 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.