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Protein

ATPase 4, plasma membrane-type

Gene

AHA4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

The plasma membrane H+ ATPase of plants and fungi generates a proton gradient that drives the active transport of nutrients by H+-symport. The resulting external acidification and/or internal alkinization may mediate growth responses (By similarity).By similarity

Catalytic activityi

ATP + H2O + H+(In) = ADP + phosphate + H+(Out).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei3374-aspartylphosphate intermediateBy similarity1
Metal bindingi596MagnesiumBy similarity1
Metal bindingi600MagnesiumBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • hydrogen-exporting ATPase activity, phosphorylative mechanism Source: TAIR
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • ATP biosynthetic process Source: InterPro
  • hydrogen ion transmembrane transport Source: GO_Central
  • regulation of intracellular pH Source: GO_Central
  • response to salt stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT3G47950-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATPase 4, plasma membrane-type (EC:3.6.3.6)
Alternative name(s):
Proton pump 4
Gene namesi
Name:AHA4
Ordered Locus Names:At3g47950
ORF Names:T17F15.180
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G47950.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 69CytoplasmicSequence analysisAdd BLAST69
Transmembranei70 – 89Helical; Name=1Sequence analysisAdd BLAST20
Topological domaini90 – 101ExtracellularSequence analysisAdd BLAST12
Transmembranei102 – 122Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini123 – 251CytoplasmicSequence analysisAdd BLAST129
Transmembranei252 – 272Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini273 – 281ExtracellularSequence analysis9
Transmembranei282 – 299Helical; Name=4Sequence analysisAdd BLAST18
Topological domaini300 – 651CytoplasmicSequence analysisAdd BLAST352
Transmembranei652 – 673Helical; Name=5Sequence analysisAdd BLAST22
Topological domaini674 – 678ExtracellularSequence analysis5
Transmembranei679 – 701Helical; Name=6Sequence analysisAdd BLAST23
Topological domaini702 – 717CytoplasmicSequence analysisAdd BLAST16
Transmembranei718 – 738Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini739 – 763ExtracellularSequence analysisAdd BLAST25
Transmembranei764 – 784Helical; Name=8Sequence analysisAdd BLAST21
Topological domaini785 – 796CytoplasmicSequence analysisAdd BLAST12
Transmembranei797 – 817Helical; Name=9Sequence analysisAdd BLAST21
Topological domaini818 – 825ExtracellularSequence analysis8
Transmembranei826 – 846Helical; Name=10Sequence analysisAdd BLAST21
Topological domaini847 – 960CytoplasmicSequence analysisAdd BLAST114

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000462771 – 960ATPase 4, plasma membrane-typeAdd BLAST960

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei893PhosphothreonineBy similarity1
Modified residuei942PhosphoserineBy similarity1
Modified residuei959PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9SU58.
PRIDEiQ9SU58.

PTM databases

iPTMnetiQ9SU58.

Expressioni

Gene expression databases

GenevisibleiQ9SU58. AT.

Interactioni

Subunit structurei

Binds to 14-3-3 proteins. The binding is induced by phosphorylation of Thr-959. Binding to 14-3-3 proteins activates the H+-ATPase (By similarity).By similarity

Protein-protein interaction databases

BioGridi9270. 1 interactor.
STRINGi3702.AT3G47950.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SU58.
SMRiQ9SU58.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni958 – 960Interaction with 14-3-3 proteinsBy similarity3

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0205. Eukaryota.
COG0474. LUCA.
HOGENOMiHOG000160005.
InParanoidiQ9SU58.
KOiK01535.
OMAiRTHVNEL.
OrthoDBiEOG093601UL.
PhylomeDBiQ9SU58.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006534. P-type_ATPase_IIIA.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01647. ATPase-IIIA_H. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SU58-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTTVEDNRE VLEAVLKEAV DLENVPIEEV FENLRCSKEG LTTQAADERL
60 70 80 90 100
ALFGHNKLEE KKESKFLKFL GFMWNPLSWV MEAAAIMAIA LANGGGKPPD
110 120 130 140 150
WQDFVGIITL LVINSTISFI EENNAGNAAA ALMARLAPKA KVLRDGRWGE
160 170 180 190 200
QDAAILVPGD IISIKLGDIV PADARLLEGD PLKIDQSALT GESLPVTKSS
210 220 230 240 250
GDGVYSGSTC KQGEIEAVVI ATGVHTFFGK AAHLVDTTNQ IGHFQQVLTA
260 270 280 290 300
IGNFCICSIA VGMLIEIVVM YPIQHRAYRP GIDNLLVLLI GGIPIAMPTV
310 320 330 340 350
LSVTMAIGSH RLSQQGAITK RMTAIEEMAG MDVLCSDKTG TLTLNKLTVD
360 370 380 390 400
KNLIEVFMKG VDADTVVLMA ARASRLENQD AIDAAIVGML ADPKDARAGI
410 420 430 440 450
QEVHFLPFNP TDKRTALTYI DNEGNTHRVS KGAPEQILNL AHNKSEIERR
460 470 480 490 500
VHAVIDKFAE RGLRSLAVAY QDVPEGRKDS AGGPWQFVGL MPLFDPPRHD
510 520 530 540 550
SAETIRRALN LGVSVKMITG DQLAIGKETG RRLGMGTNMY PSSALLGQNK
560 570 580 590 600
DESIVALPVD ELIEKADGFA GVFPEHKYEI VKRLQARKHI CGMTGDGVND
610 620 630 640 650
APALKKADIG IAVADATDAA RSASDIVLTE PGLSVIISAV LTSRAIFQRM
660 670 680 690 700
KNYTIYAVSI TIRIVLGFML LALIWQFDFP PFMVLIIAIL NDGTIMTISK
710 720 730 740 750
DRVKPSPLPD SWKLSEIFAT GVVFGSYMAM MTVIFFWVSY KTDFFPRTFG
760 770 780 790 800
VATLEKTAHD DFRKLASAIY LQVSIISQAL IFVTRSRSWS FVERPGIFLM
810 820 830 840 850
IAFILAQLVA TLIAVYANWS FAAIEGIGWG WAGVIWLYNI IFYIPLDFIK
860 870 880 890 900
FFIRYALSGR AWDLVIEQRV AFTRQKDFGK EQRELQWAHA QRTLHGLQAP
910 920 930 940 950
DTKMFTDRTH VSELNQMAEE AKRRAEIARL RELHTLKGHV ESVVRLKGLD
960
IETIQQAYTV
Length:960
Mass (Da):105,718
Last modified:December 6, 2005 - v2
Checksum:iB2E9548A74606E39
GO

Sequence cautioni

The sequence CAB41144 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL049658 Genomic DNA. Translation: CAB41144.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE78350.1.
AK118088 mRNA. Translation: BAC42716.1.
PIRiT06688.
RefSeqiNP_190378.2. NM_114664.4.
UniGeneiAt.20263.
At.71210.

Genome annotation databases

EnsemblPlantsiAT3G47950.1; AT3G47950.1; AT3G47950.
GeneIDi823950.
GrameneiAT3G47950.1; AT3G47950.1; AT3G47950.
KEGGiath:AT3G47950.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL049658 Genomic DNA. Translation: CAB41144.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE78350.1.
AK118088 mRNA. Translation: BAC42716.1.
PIRiT06688.
RefSeqiNP_190378.2. NM_114664.4.
UniGeneiAt.20263.
At.71210.

3D structure databases

ProteinModelPortaliQ9SU58.
SMRiQ9SU58.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi9270. 1 interactor.
STRINGi3702.AT3G47950.1.

PTM databases

iPTMnetiQ9SU58.

Proteomic databases

PaxDbiQ9SU58.
PRIDEiQ9SU58.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G47950.1; AT3G47950.1; AT3G47950.
GeneIDi823950.
GrameneiAT3G47950.1; AT3G47950.1; AT3G47950.
KEGGiath:AT3G47950.

Organism-specific databases

TAIRiAT3G47950.

Phylogenomic databases

eggNOGiKOG0205. Eukaryota.
COG0474. LUCA.
HOGENOMiHOG000160005.
InParanoidiQ9SU58.
KOiK01535.
OMAiRTHVNEL.
OrthoDBiEOG093601UL.
PhylomeDBiQ9SU58.

Enzyme and pathway databases

BioCyciARA:AT3G47950-MONOMER.

Miscellaneous databases

PROiQ9SU58.

Gene expression databases

GenevisibleiQ9SU58. AT.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006534. P-type_ATPase_IIIA.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01647. ATPase-IIIA_H. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPMA4_ARATH
AccessioniPrimary (citable) accession number: Q9SU58
Secondary accession number(s): Q8GXR3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: December 6, 2005
Last modified: November 30, 2016
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.