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Protein

Origin of replication complex subunit 1B

Gene

ORC1B

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential protein required for ovules fertilization (PubMed:15634699, PubMed:19171893). Component of the origin recognition complex (ORC) that binds origins of replication. It has a role in both chromosomal replication and mating type transcriptional silencing. Binds to the ARS consensus sequence (ACS) of origins of replication (By similarity). H3K4me3 effector that regulates positively the transcription of a subset of genes (PubMed:19171893).By similarity2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri166 – 21550PHD-typePROSITE-ProRule annotationAdd
BLAST
Nucleotide bindingi471 – 4788ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

  • DNA replication Source: TAIR
  • double fertilization forming a zygote and endosperm Source: TAIR
  • regulation of transcription, DNA-templated Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

DNA replication, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, DNA-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-113510. E2F mediated regulation of DNA replication.
R-ATH-68616. Assembly of the ORC complex at the origin of replication.
R-ATH-68689. CDC6 association with the ORC:origin complex.
R-ATH-68867. Assembly of the pre-replicative complex.
R-ATH-68949. Orc1 removal from chromatin.
R-ATH-68962. Activation of the pre-replicative complex.
R-ATH-69205. G1/S-Specific Transcription.
R-ATH-69298. Association of licensing factors with the pre-replicative complex.

Names & Taxonomyi

Protein namesi
Recommended name:
Origin of replication complex subunit 1B1 Publication
Short name:
AtORC1b1 Publication
Alternative name(s):
Origin recognition complex 1b protein
Protein UNFERTILIZED EMBRYO SAC 13
Gene namesi
Name:ORC1B1 Publication
Synonyms:UNE131 Publication
Ordered Locus Names:At4g12620Imported
ORF Names:T1P17.210Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)Imported
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G12620.

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

  • nucleus Source: UniProtKB-SubCell
  • origin recognition complex Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Lethal (PubMed:19171893). Unfertilized ovules but normal pollen tube attraction (PubMed:15634699).1 Publication1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi183 – 1864CDDC → ADDA in ORC1b-PHD(C/A); reduced H3K4me3 interaction. 1 Publication
Mutagenesisi190 – 1901F → A in ORC1b-PHD(F/A); reduced H3K4me3 interaction. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 813813Origin of replication complex subunit 1BPRO_0000431427Add
BLAST

Proteomic databases

PaxDbiQ9SU24.
PRIDEiQ9SU24.

PTM databases

iPTMnetiQ9SU24.

Expressioni

Tissue specificityi

Follow a cell-cycle regulation with a peak at the G1/S-phase (PubMed:16179646). Mostly expressed in flower buds, and, to a lower exent, in roots, leaves and stems (PubMed:16179646, PubMed:15358564).2 Publications

Developmental stagei

Restricted to proliferating cells. First active during embryogenesis, but inactive in mature embryos. Expressed in shoot and root apical meristems. Later observed in lateral root primordia and meristems. Detected in young flower buds, developing anthers and mature pollen.1 Publication

Inductioni

Regulated by E2F (PubMed:16179646, PubMed:16126853). Accumulates rapidly after cell cycle reactivation by sucrose addition following cell cycle arrest mediated by sucrose deprivation (PubMed:16179646, PubMed:15358564).3 Publications

Gene expression databases

GenevisibleiQ9SU24. AT.

Interactioni

Subunit structurei

Component of the origin recognition complex (ORC) composed of at least ORC1 (ORC1A or ORC1B), ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle and development dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase. Interacts directly with ORC2 and ORC5 (PubMed:16179646, PubMed:15358564). Binds H3K4me3 (PubMed:19171893).3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ABAP1B7U1792EBI-2114228,EBI-541722

Protein-protein interaction databases

DIPiDIP-46671N.
IntActiQ9SU24. 4 interactions.
MINTiMINT-7303885.
STRINGi3702.AT4G12620.1.

Structurei

Secondary structure

1
813
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi129 – 1379Combined sources
Beta strandi140 – 1434Combined sources
Beta strandi147 – 1504Combined sources
Turni170 – 1723Combined sources
Beta strandi180 – 1823Combined sources
Turni184 – 1863Combined sources
Beta strandi189 – 1913Combined sources
Helixi192 – 1943Combined sources
Beta strandi195 – 1973Combined sources
Helixi210 – 2145Combined sources
Helixi238 – 2436Combined sources
Beta strandi246 – 25813Combined sources
Turni259 – 2613Combined sources
Beta strandi264 – 2729Combined sources
Helixi274 – 2763Combined sources
Beta strandi291 – 30010Combined sources
Helixi301 – 3033Combined sources
Beta strandi304 – 3118Combined sources
Helixi313 – 3164Combined sources
Helixi317 – 3193Combined sources
Beta strandi326 – 3283Combined sources
Beta strandi331 – 3344Combined sources
Turni335 – 3384Combined sources
Beta strandi339 – 3424Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5HH7X-ray1.90A118-349[»]
ProteinModelPortaliQ9SU24.
SMRiQ9SU24. Positions 169-245, 440-801.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini226 – 344119BAHPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni436 – 803368Necessary and sufficient for ORC complex assemblyBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi102 – 1065Poly-LysSequence analysis
Compositional biasi122 – 1287Poly-LysSequence analysis

Sequence similaritiesi

Belongs to the ORC1 family.Curated
Contains 1 BAH domain.PROSITE-ProRule annotation
Contains 1 PHD-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri166 – 21550PHD-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1514. Eukaryota.
COG1474. LUCA.
HOGENOMiHOG000030288.
InParanoidiQ9SU24.
KOiK02603.
OMAiEMECILR.
PhylomeDBiQ9SU24.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR001025. BAH_dom.
IPR027417. P-loop_NTPase.
IPR019786. Zinc_finger_PHD-type_CS.
IPR011011. Znf_FYVE_PHD.
IPR001965. Znf_PHD.
IPR019787. Znf_PHD-finger.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF01426. BAH. 1 hit.
PF00628. PHD. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00439. BAH. 1 hit.
SM00249. PHD. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF57903. SSF57903. 1 hit.
PROSITEiPS51038. BAH. 2 hits.
PS01359. ZF_PHD_1. 1 hit.
PS50016. ZF_PHD_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SU24-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASTPRAKTF KSPTKTPSNI YRKSYLSPSS TSHTPQTPET HTPLRRSARH
60 70 80 90 100
VSRKIDLGND PIDAPGNDPI EGMNLIRKRE RAPRKPTTDV VPSKSKKTET
110 120 130 140 150
PKKKKKIDSF TPVSPIRSET IKKTKKKKRV YYNKVEFDET EFEIGDDVYV
160 170 180 190 200
KRREDSNSDE EEDPEIEDCQ ICFKSDTNIM IECDDCLGGF HLKCLKPPLK
210 220 230 240 250
EVPEGDWICQ FCEVKKSGQS QTLDLPKPPE GKKLARTMRE KLLSGDLWAA
260 270 280 290 300
RIDKLWKEVD DGVYWIRARW YMIPEETVSG RQPHNLKREL YLTNDFADIE
310 320 330 340 350
MECILRHCSV KCPKEFSKAS NDGDDVFLCE YEYDVHWRSF KRLAELADGD
360 370 380 390 400
SDSDQEWNGR KEEEVDDSDE EMELDDEVLK SKRGGLTSAR GGANSRKGRF
410 420 430 440 450
FGVEKVGMKL IPEHVRCHKQ SELEKAKATL LLATRPKSLP CRSKEMEEIT
460 470 480 490 500
SFIKGSISDD QCLGRCMYIH GVPGTGKTIS VLSVMKNLKA EVEEGSVSPY
510 520 530 540 550
CFVEINGLKL ASPENIYSVI YEALSGHRVG WKKALQCLNE RFAEGKRIGK
560 570 580 590 600
EDEKPCILLI DELDLLVTRN QSVLYNILDW PTKPNSKLVV LGIANTMDLP
610 620 630 640 650
EKLLPRISSR MGIQRLCFGP YNHTQLQEII STRLNGIDAF EKTAIEFASR
660 670 680 690 700
KVAAISGDAR RALEICRRAA EVADHRLNTN KSAKNQLVIM ADVEAAIQEM
710 720 730 740 750
FQAPHIQVMK SVSKLSKIFL TAMVHELYKT GMAETTFDRV ATTVSSICLT
760 770 780 790 800
NGEAFPGWDI LLKIGCDLGE CRIILCEPGE KHRLQKLQLN FPSDDVAFAL
810
KDNKDLPWLA NYL
Length:813
Mass (Da):92,170
Last modified:May 1, 2000 - v1
Checksum:i5F732C91B00944A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ426477 mRNA. Translation: CAD20132.1.
AL049730 Genomic DNA. Translation: CAB53755.1.
AL161534 Genomic DNA. Translation: CAB78305.1.
CP002687 Genomic DNA. Translation: AEE83158.1.
PIRiE85135.
RefSeqiNP_192999.1. NM_117332.2.
UniGeneiAt.48852.

Genome annotation databases

EnsemblPlantsiAT4G12620.1; AT4G12620.1; AT4G12620.
GeneIDi826875.
GrameneiAT4G12620.1; AT4G12620.1; AT4G12620.
KEGGiath:AT4G12620.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ426477 mRNA. Translation: CAD20132.1.
AL049730 Genomic DNA. Translation: CAB53755.1.
AL161534 Genomic DNA. Translation: CAB78305.1.
CP002687 Genomic DNA. Translation: AEE83158.1.
PIRiE85135.
RefSeqiNP_192999.1. NM_117332.2.
UniGeneiAt.48852.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5HH7X-ray1.90A118-349[»]
ProteinModelPortaliQ9SU24.
SMRiQ9SU24. Positions 169-245, 440-801.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-46671N.
IntActiQ9SU24. 4 interactions.
MINTiMINT-7303885.
STRINGi3702.AT4G12620.1.

PTM databases

iPTMnetiQ9SU24.

Proteomic databases

PaxDbiQ9SU24.
PRIDEiQ9SU24.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G12620.1; AT4G12620.1; AT4G12620.
GeneIDi826875.
GrameneiAT4G12620.1; AT4G12620.1; AT4G12620.
KEGGiath:AT4G12620.

Organism-specific databases

TAIRiAT4G12620.

Phylogenomic databases

eggNOGiKOG1514. Eukaryota.
COG1474. LUCA.
HOGENOMiHOG000030288.
InParanoidiQ9SU24.
KOiK02603.
OMAiEMECILR.
PhylomeDBiQ9SU24.

Enzyme and pathway databases

ReactomeiR-ATH-113510. E2F mediated regulation of DNA replication.
R-ATH-68616. Assembly of the ORC complex at the origin of replication.
R-ATH-68689. CDC6 association with the ORC:origin complex.
R-ATH-68867. Assembly of the pre-replicative complex.
R-ATH-68949. Orc1 removal from chromatin.
R-ATH-68962. Activation of the pre-replicative complex.
R-ATH-69205. G1/S-Specific Transcription.
R-ATH-69298. Association of licensing factors with the pre-replicative complex.

Miscellaneous databases

PROiQ9SU24.

Gene expression databases

GenevisibleiQ9SU24. AT.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR001025. BAH_dom.
IPR027417. P-loop_NTPase.
IPR019786. Zinc_finger_PHD-type_CS.
IPR011011. Znf_FYVE_PHD.
IPR001965. Znf_PHD.
IPR019787. Znf_PHD-finger.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF01426. BAH. 1 hit.
PF00628. PHD. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00439. BAH. 1 hit.
SM00249. PHD. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF57903. SSF57903. 1 hit.
PROSITEiPS51038. BAH. 2 hits.
PS01359. ZF_PHD_1. 1 hit.
PS50016. ZF_PHD_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genes encoding Arabidopsis ORC subunits are E2F targets and the two ORC1 genes are differently expressed in proliferating and endoreplicating cells."
    Diaz-Trivino S., Castellano M.M., Sanchez M.P., Ramirez-Parra E., Desvoyes B., Gutierrez C.
    Nucleic Acids Res. 33:5404-5414(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT, INTERACTION WITH ORC2 AND ORC5, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION BY E2F AND SUCROSE, GENE FAMILY, NOMENCLATURE.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Genome based identification and analysis of the pre-replicative complex of Arabidopsis thaliana."
    Masuda H.P., Ramos G.B.A., de Almeida-Engler J., Cabral L.M., Coqueiro V.M., Macrini C.M.T., Ferreira P.C.G., Hemerly A.S.
    FEBS Lett. 574:192-202(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, TISSUE SPECIFICITY, INDUCTION BY SUCROSE, GENE FAMILY.
    Strain: cv. Columbia.
  5. "Genetic and molecular identification of genes required for female gametophyte development and function in Arabidopsis."
    Pagnussat G.C., Yu H.-J., Ngo Q.A., Rajani S., Mayalagu S., Johnson C.S., Capron A., Xie L.-F., Ye D., Sundaresan V.
    Development 132:603-614(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  6. Cited for: INDUCTION BY E2F.
  7. "Genome-wide analysis of the core DNA replication machinery in the higher plants Arabidopsis and rice."
    Shultz R.W., Tatineni V.M., Hanley-Bowdoin L., Thompson W.F.
    Plant Physiol. 144:1697-1714(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW ON THE CORE DNA REPLICATION MACHINERY.
  8. "Arabidopsis ORC1 is a PHD-containing H3K4me3 effector that regulates transcription."
    de la Paz Sanchez M., Gutierrez C.
    Proc. Natl. Acad. Sci. U.S.A. 106:2065-2070(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH H3K4ME3, MUTAGENESIS OF 183-CYS--CYS-186 AND PHE-190.

Entry informationi

Entry nameiORC1B_ARATH
AccessioniPrimary (citable) accession number: Q9SU24
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 7, 2015
Last sequence update: May 1, 2000
Last modified: June 8, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.