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Protein

Origin of replication complex subunit 1B

Gene

ORC1B

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential protein required for ovules fertilization (PubMed:15634699, PubMed:19171893). Component of the origin recognition complex (ORC) that binds origins of replication. It has a role in both chromosomal replication and mating type transcriptional silencing. Binds to the ARS consensus sequence (ACS) of origins of replication (By similarity). H3K4me3 effector that regulates positively the transcription of a subset of genes (PubMed:19171893).By similarity2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri166 – 215PHD-typePROSITE-ProRule annotationAdd BLAST50
Nucleotide bindingi471 – 478ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

  • DNA replication Source: TAIR
  • double fertilization forming a zygote and endosperm Source: TAIR
  • regulation of transcription, DNA-templated Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, DNA-binding
Biological processDNA replication, Transcription, Transcription regulation
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-68616 Assembly of the ORC complex at the origin of replication
R-ATH-68689 CDC6 association with the ORC:origin complex
R-ATH-68867 Assembly of the pre-replicative complex
R-ATH-68949 Orc1 removal from chromatin
R-ATH-68962 Activation of the pre-replicative complex

Names & Taxonomyi

Protein namesi
Recommended name:
Origin of replication complex subunit 1B1 Publication
Short name:
AtORC1b1 Publication
Alternative name(s):
Origin recognition complex 1b protein
Protein UNFERTILIZED EMBRYO SAC 13
Gene namesi
Name:ORC1B1 Publication
Synonyms:UNE131 Publication
Ordered Locus Names:At4g12620Imported
ORF Names:T1P17.210Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)Imported
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G12620
TAIRilocus:2135575 AT4G12620

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Lethal (PubMed:19171893). Unfertilized ovules but normal pollen tube attraction (PubMed:15634699).1 Publication1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi183 – 186CDDC → ADDA in ORC1b-PHD(C/A); reduced H3K4me3 interaction. 1 Publication4
Mutagenesisi190F → A in ORC1b-PHD(F/A); reduced H3K4me3 interaction. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004314271 – 813Origin of replication complex subunit 1BAdd BLAST813

Proteomic databases

PaxDbiQ9SU24
PRIDEiQ9SU24

PTM databases

iPTMnetiQ9SU24

Expressioni

Tissue specificityi

Follow a cell-cycle regulation with a peak at the G1/S-phase (PubMed:16179646). Mostly expressed in flower buds, and, to a lower exent, in roots, leaves and stems (PubMed:16179646, PubMed:15358564).2 Publications

Developmental stagei

Restricted to proliferating cells. First active during embryogenesis, but inactive in mature embryos. Expressed in shoot and root apical meristems. Later observed in lateral root primordia and meristems. Detected in young flower buds, developing anthers and mature pollen.1 Publication

Inductioni

Regulated by E2F (PubMed:16179646, PubMed:16126853). Accumulates rapidly after cell cycle reactivation by sucrose addition following cell cycle arrest mediated by sucrose deprivation (PubMed:16179646, PubMed:15358564).3 Publications

Gene expression databases

ExpressionAtlasiQ9SU24 baseline and differential
GenevisibleiQ9SU24 AT

Interactioni

Subunit structurei

Component of the origin recognition complex (ORC) composed of at least ORC1 (ORC1A or ORC1B), ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle and development dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase. Interacts directly with ORC2 and ORC5 (PubMed:16179646, PubMed:15358564). Binds H3K4me3 (PubMed:19171893).3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ABAP1B7U1792EBI-2114228,EBI-541722

Protein-protein interaction databases

DIPiDIP-46671N
IntActiQ9SU24, 9 interactors
MINTiQ9SU24
STRINGi3702.AT4G12620.1

Structurei

Secondary structure

1813
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi129 – 137Combined sources9
Beta strandi140 – 143Combined sources4
Beta strandi147 – 150Combined sources4
Turni170 – 172Combined sources3
Beta strandi180 – 182Combined sources3
Turni184 – 186Combined sources3
Beta strandi189 – 191Combined sources3
Helixi192 – 194Combined sources3
Beta strandi195 – 197Combined sources3
Helixi210 – 214Combined sources5
Helixi238 – 243Combined sources6
Beta strandi246 – 258Combined sources13
Turni259 – 261Combined sources3
Beta strandi264 – 272Combined sources9
Helixi274 – 276Combined sources3
Beta strandi291 – 300Combined sources10
Helixi301 – 303Combined sources3
Beta strandi304 – 311Combined sources8
Helixi313 – 316Combined sources4
Helixi317 – 319Combined sources3
Beta strandi326 – 328Combined sources3
Beta strandi331 – 334Combined sources4
Turni335 – 338Combined sources4
Beta strandi339 – 342Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5HH7X-ray1.90A118-349[»]
ProteinModelPortaliQ9SU24
SMRiQ9SU24
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini226 – 344BAHPROSITE-ProRule annotationAdd BLAST119

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni436 – 803Necessary and sufficient for ORC complex assemblyBy similarityAdd BLAST368

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi102 – 106Poly-LysSequence analysis5
Compositional biasi122 – 128Poly-LysSequence analysis7

Sequence similaritiesi

Belongs to the ORC1 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri166 – 215PHD-typePROSITE-ProRule annotationAdd BLAST50

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1514 Eukaryota
COG1474 LUCA
HOGENOMiHOG000030288
InParanoidiQ9SU24
KOiK02603
OMAiHIESIWK
OrthoDBiEOG0936037M
PhylomeDBiQ9SU24

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR003959 ATPase_AAA_core
IPR001025 BAH_dom
IPR020793 ORC1
IPR027417 P-loop_NTPase
IPR019786 Zinc_finger_PHD-type_CS
IPR011011 Znf_FYVE_PHD
IPR001965 Znf_PHD
IPR019787 Znf_PHD-finger
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR10763:SF23 PTHR10763:SF23, 1 hit
PfamiView protein in Pfam
PF00004 AAA, 1 hit
PF01426 BAH, 1 hit
PF00628 PHD, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SM00439 BAH, 1 hit
SM00249 PHD, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
SSF57903 SSF57903, 1 hit
PROSITEiView protein in PROSITE
PS51038 BAH, 2 hits
PS01359 ZF_PHD_1, 1 hit
PS50016 ZF_PHD_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9SU24-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASTPRAKTF KSPTKTPSNI YRKSYLSPSS TSHTPQTPET HTPLRRSARH
60 70 80 90 100
VSRKIDLGND PIDAPGNDPI EGMNLIRKRE RAPRKPTTDV VPSKSKKTET
110 120 130 140 150
PKKKKKIDSF TPVSPIRSET IKKTKKKKRV YYNKVEFDET EFEIGDDVYV
160 170 180 190 200
KRREDSNSDE EEDPEIEDCQ ICFKSDTNIM IECDDCLGGF HLKCLKPPLK
210 220 230 240 250
EVPEGDWICQ FCEVKKSGQS QTLDLPKPPE GKKLARTMRE KLLSGDLWAA
260 270 280 290 300
RIDKLWKEVD DGVYWIRARW YMIPEETVSG RQPHNLKREL YLTNDFADIE
310 320 330 340 350
MECILRHCSV KCPKEFSKAS NDGDDVFLCE YEYDVHWRSF KRLAELADGD
360 370 380 390 400
SDSDQEWNGR KEEEVDDSDE EMELDDEVLK SKRGGLTSAR GGANSRKGRF
410 420 430 440 450
FGVEKVGMKL IPEHVRCHKQ SELEKAKATL LLATRPKSLP CRSKEMEEIT
460 470 480 490 500
SFIKGSISDD QCLGRCMYIH GVPGTGKTIS VLSVMKNLKA EVEEGSVSPY
510 520 530 540 550
CFVEINGLKL ASPENIYSVI YEALSGHRVG WKKALQCLNE RFAEGKRIGK
560 570 580 590 600
EDEKPCILLI DELDLLVTRN QSVLYNILDW PTKPNSKLVV LGIANTMDLP
610 620 630 640 650
EKLLPRISSR MGIQRLCFGP YNHTQLQEII STRLNGIDAF EKTAIEFASR
660 670 680 690 700
KVAAISGDAR RALEICRRAA EVADHRLNTN KSAKNQLVIM ADVEAAIQEM
710 720 730 740 750
FQAPHIQVMK SVSKLSKIFL TAMVHELYKT GMAETTFDRV ATTVSSICLT
760 770 780 790 800
NGEAFPGWDI LLKIGCDLGE CRIILCEPGE KHRLQKLQLN FPSDDVAFAL
810
KDNKDLPWLA NYL
Length:813
Mass (Da):92,170
Last modified:May 1, 2000 - v1
Checksum:i5F732C91B00944A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ426477 mRNA Translation: CAD20132.1
AL049730 Genomic DNA Translation: CAB53755.1
AL161534 Genomic DNA Translation: CAB78305.1
CP002687 Genomic DNA Translation: AEE83158.1
PIRiE85135
RefSeqiNP_192999.1, NM_117332.3
UniGeneiAt.48852

Genome annotation databases

EnsemblPlantsiAT4G12620.1; AT4G12620.1; AT4G12620
GeneIDi826875
GrameneiAT4G12620.1; AT4G12620.1; AT4G12620
KEGGiath:AT4G12620

Similar proteinsi

Entry informationi

Entry nameiORC1B_ARATH
AccessioniPrimary (citable) accession number: Q9SU24
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 7, 2015
Last sequence update: May 1, 2000
Last modified: May 23, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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