Reviewed, UniProtKB/Swiss-Prot Q9SU14 (NUDT7_ARATH)

Names and origin

Protein names

Recommended name:
    Nudix hydrolase 7
      Short name=AtNUDT7
    EC=3.6.1.-
Alternative name(s):
    ADP-ribose pyrophosphatase
    EC=3.6.1.13
    NADH pyrophosphatase
    EC=3.6.1.22
    Protein GROWTH FACTOR GENE 1

Gene names

Name:	NUDT7
Synonyms:	GFG1, NUDX7
Ordered Locus Names:	At4g12720
ORF Names:	T20K18.70

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Mediates the hydrolysis of some nucleoside diphosphate derivatives. Can use both NADH and ADP-ribose as substrates, but not 8-oxo-dGTP, cyclic ADP-ribose, GDP-manose, UDP-glucose, ATP, or GTP. Exerts negative control of EDS1 signaling.
Catalytic activity	ADP-ribose + H2O = AMP + D-ribose 5-phosphate. NAD+ + H2O = AMP + NMN.
Cofactor	Magnesium.
Enzyme regulation	Not inhibited by fluoride.
Subunit structure	Homodimer.
Tissue specificity	Expressed in stems, leaves, roots, flowers and siliques.
Induction	Rapid and transient induction by biotic and abiotic stresses. Not induced by H2O2.
Disruption phenotype	Growth retardation, constitutive pathogen resistance phenotype and increased levels of reactive oxygen species and NADH.
Sequence similarities	Belongs to the Nudix hydrolase family. Contains 1 nudix hydrolase domain.
Biophysicochemical properties	Kinetic parameters: KM=26.23 µM for NADH KM=25.8 µM for ADP-ribose Vmax=3.681 nmol/min/mg enzyme with NADH as substrate Vmax=2678 nmol/min/mg enzyme with ADP-ribose as substrate pH dependence: Optimum pH is 8.5. Temperature dependence: Optimum temperature is 50 degrees Celsius.

Ontologies

Keywords
Ligand	Magnesium Metal-binding NAD
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	defense response signaling pathway, resistance gene-dependent Ref.6 Inferred from mutant phenotype. Source: TAIR defense response to bacterium Ref.8 Inferred from mutant phenotype. Source: TAIR plant-type hypersensitive response Ref.6 Inferred from mutant phenotype. Source: TAIR response to oxidative stress Ref.7 Inferred from expression pattern. Source: TAIR response to ozone Ref.7 Inferred from expression pattern. Source: TAIR response to salt stress Ref.7 Inferred from expression pattern. Source: TAIR
Molecular function	ADP-ribose diphosphatase activity Ref.4 Ref.5 Inferred from direct assay. Source: TAIR NAD or NADH binding Ref.4 Inferred from direct assay. Source: TAIR NAD+ diphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-diphosphatase activity Ref.8 Inferred from direct assay. Source: TAIR protein homodimerization activity Ref.5 Inferred from direct assay. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 282	282	Nudix hydrolase 7		PRO_0000057127
Regions
	Domain	102 – 237	136	Nudix hydrolase
	Motif	139 – 160	22	Nudix box
Sites
	Metal binding	154	1	Magnesium By similarity
	Metal binding	158	1	Magnesium By similarity
Experimental info
	Mutagenesis	154	1	E → Q: Loss of hydrolase acitvity.
	Sequence conflict	6	1	Q → L in AAM62604. Ref.3
	Sequence conflict	217	1	V → I in AAM62604. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9SU14-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: EA37FC0F1C2FDCB7	FASTA	282	31,884
	10 20 30 40 50 60 MGTRAQQIPL LEGETDNYDG VTVTMVEPMD SEVFTESLRA SLSHWREEGK KGIWIKLPLG 70 80 90 100 110 120 LANLVEAAVS EGFRYHHAEP EYLMLVSWIS ETPDTIPANA SHVVGAGALV INKNTKEVLV 130 140 150 160 170 180 VQERSGFFKD KNVWKLPTGV INEGEDIWTG VAREVEEETG IIADFVEVLA FRQSHKAILK 190 200 210 220 230 240 KKTDMFFLCV LSPRSYDITE QKSEILQAKW MPIQEYVDQP WNKKNEMFKF MANICQKKCE 250 260 270 280 EEYLGFAIVP TTTSSGKESF IYCNADHAKR LKVSRDQASA SL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Comprehensive analysis of cytosolic nudix hydrolases in Arabidopsis thaliana." Ogawa T., Ueda Y., Yoshimura K., Shigeoka S. J. Biol. Chem. 280:25277-25283(2005) [PubMed: 15878881] [Abstract] Cited for: FUNCTION IN VITRO, TISSUE SPECIFICITY.
[5]	"Cloning and characterization of an Arabidopsis thaliana Nudix hydrolase homologous to the mammalian GFG protein." Olejnik K., Kraszewska E. Biochim. Biophys. Acta 1752:133-141(2005) [PubMed: 16154395] [Abstract] Cited for: FUNCTION, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.
[6]	"Salicylic acid-independent ENHANCED DISEASE SUSCEPTIBILITY1 signaling in Arabidopsis immunity and cell death is regulated by the monooxygenase FMO1 and the Nudix hydrolase NUDT7." Bartsch M., Gobbato E., Bednarek P., Debey S., Schultze J.L., Bautor J., Parker J.E. Plant Cell 18:1038-1051(2006) [PubMed: 16531493] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[7]	"Analysis of Arabidopsis growth factor gene 1 (GFG1) encoding a nudix hydrolase during oxidative signaling." Jambunathan N., Mahalingam R. Planta 224:1-11(2006) [PubMed: 16328543] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION, BIOPHYSICOCHEMICAL PROPERTIES, DISRUPTION PHENOTYPE.
[8]	"AtNUDT7, a negative regulator of basal immunity in Arabidopsis, modulates two distinct defense response pathways and is involved in maintaining redox homeostasis." Ge X., Li G.-J., Wang S.-B., Zhu H., Zhu T., Wang X., Xia Y. Plant Physiol. 145:204-215(2007) [PubMed: 17660350] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF GLU-154, INDUCTION, DISRUPTION PHENOTYPE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL049640 Genomic DNA. Translation: CAB40989.1. AL161534 Genomic DNA. Translation: CAB78314.1. AF325104 mRNA. Translation: AAK17172.1. AF370209 mRNA. Translation: AAK44024.1. AY056344 mRNA. Translation: AAL07193.1. AY085375 mRNA. Translation: AAM62604.1.
IPI	IPI00537463.
PIR	T06630.
RefSeq	NP_193008.1. NP_849367.1. NP_849368.1.
UniGene	At.23449
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q9SU14.
ProMEX	Q9SU14.
Genome annotation databases
GeneID	826884.
GenomeReviews	Gene locus AT4G12720 in contig CT486007_GR.
KEGG	ath:AT4G12720.
NMPDR	fig|3702.1.peg.18947.
Organism-specific databases
TAIR	At4g12720.
Phylogenomic databases
OMA	Q9SU14. ANASHIV.
Enzyme and pathway databases
BRENDA	3.6.1.13. 302. 3.6.1.22. 302.
Gene expression databases
GermOnline	AT4G12720. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR003293. GFG_antis. IPR000086. NUDIX_hydrolase_core. [Graphical view]
Gene3D	G3DSA:3.90.79.10. NUDIX_hydrolase. 1 hit.
Pfam	PF00293. NUDIX. 1 hit. [Graphical view]
PRINTS	PR01356. GFGPROTEIN.
PROSITE	PS00893. NUDIX. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUDT7_ARATH

Accession

Primary (citable) accession number: Q9SU14
Secondary accession number(s): Q8LEK5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	May 1, 2000
Last modified:	July 7, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)