Q9SU14 (NUDT7_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nudix hydrolase 7
Short name=AtNUDT7
EC=3.6.1.-

Alternative name(s):
ADP-ribose pyrophosphatase
EC=3.6.1.13
NADH pyrophosphatase
EC=3.6.1.22
Protein GROWTH FACTOR GENE 1

Gene names

Name:	NUDT7
Synonyms:	GFG1, NUDX7
Ordered Locus Names:	At4g12720
ORF Names:	T20K18.70

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mediates the hydrolysis of some nucleoside diphosphate derivatives. Can use both NADH and ADP-ribose as substrates, but not 8-oxo-dGTP, cyclic ADP-ribose, GDP-manose, UDP-glucose, ATP, or GTP. Exerts negative control of EDS1 signaling. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9
Catalytic activity	ADP-ribose + H2O = AMP + D-ribose 5-phosphate. NAD+ + H2O = AMP + NMN.
Cofactor	Magnesium.
Enzyme regulation	Not inhibited by fluoride.
Subunit structure	Homodimer. Ref.6
Tissue specificity	Expressed in stems, leaves, roots, flowers and siliques. Ref.5 Ref.8
Induction	Rapid and transient induction by biotic and abiotic stresses. Not induced by H2O2. Ref.8 Ref.9
Disruption phenotype	Growth retardation, constitutive pathogen resistance phenotype and increased levels of reactive oxygen species and NADH. Ref.7 Ref.8 Ref.9
Sequence similarities	Belongs to the Nudix hydrolase family. Contains 1 nudix hydrolase domain.
Biophysicochemical properties	Kinetic parameters: KM=26.23 µM for NADH Ref.6 Ref.8 KM=25.8 µM for ADP-ribose Vmax=3.681 nmol/min/mg enzyme with NADH as substrate Vmax=2678 nmol/min/mg enzyme with ADP-ribose as substrate pH dependence: Optimum pH is 8.5. Temperature dependence: Optimum temperature is 50 degrees Celsius.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	Magnesium Metal-binding NAD
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	defense response signaling pathway, resistance gene-dependent Inferred from mutant phenotype Ref.7. Source: TAIR defense response to bacterium Inferred from mutant phenotype Ref.9. Source: TAIR plant-type hypersensitive response Inferred from mutant phenotype Ref.7. Source: TAIR protein poly-ADP-ribosylation Inferred from direct assay. Source: TAIR response to oxidative stress Inferred from expression pattern Ref.8. Source: TAIR response to ozone Inferred from expression pattern Ref.8. Source: TAIR response to salt stress Inferred from expression pattern Ref.8. Source: TAIR
Cellular component	cytosol Inferred from direct assay. Source: TAIR
Molecular function	ADP-ribose diphosphatase activity Inferred from direct assay Ref.5Ref.6. Source: TAIR NAD binding Inferred from direct assay Ref.5. Source: TAIR NAD+ diphosphatase activity Inferred from direct assay. Source: TAIR metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-diphosphatase activity Inferred from direct assay Ref.9. Source: TAIR protein homodimerization activity Inferred from direct assay Ref.6. Source: TAIR
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9SU14-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 282	282	Nudix hydrolase 7		PRO_0000057127
Regions
	Domain	101 – 233	133	Nudix hydrolase
	Motif	139 – 160	22	Nudix box
Sites
	Metal binding	154	1	Magnesium By similarity
	Metal binding	158	1	Magnesium By similarity
Experimental info
	Mutagenesis	154	1	E → Q: Loss of hydrolase acitvity. Ref.9
	Sequence conflict	6	1	Q → L in AAM62604. Ref.4
	Sequence conflict	217	1	V → I in AAM62604. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: EA37FC0F1C2FDCB7	FASTA	282	31,884
	10 20 30 40 50 60 MGTRAQQIPL LEGETDNYDG VTVTMVEPMD SEVFTESLRA SLSHWREEGK KGIWIKLPLG 70 80 90 100 110 120 LANLVEAAVS EGFRYHHAEP EYLMLVSWIS ETPDTIPANA SHVVGAGALV INKNTKEVLV 130 140 150 160 170 180 VQERSGFFKD KNVWKLPTGV INEGEDIWTG VAREVEEETG IIADFVEVLA FRQSHKAILK 190 200 210 220 230 240 KKTDMFFLCV LSPRSYDITE QKSEILQAKW MPIQEYVDQP WNKKNEMFKF MANICQKKCE 250 260 270 280 EEYLGFAIVP TTTSSGKESF IYCNADHAKR LKVSRDQASA SL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Comprehensive analysis of cytosolic nudix hydrolases in Arabidopsis thaliana." Ogawa T., Ueda Y., Yoshimura K., Shigeoka S. J. Biol. Chem. 280:25277-25283(2005) [PubMed: 15878881] [Abstract] Cited for: FUNCTION IN VITRO, TISSUE SPECIFICITY.
[6]	"Cloning and characterization of an Arabidopsis thaliana Nudix hydrolase homologous to the mammalian GFG protein." Olejnik K., Kraszewska E. Biochim. Biophys. Acta 1752:133-141(2005) [PubMed: 16154395] [Abstract] Cited for: FUNCTION, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.
[7]	"Salicylic acid-independent ENHANCED DISEASE SUSCEPTIBILITY1 signaling in Arabidopsis immunity and cell death is regulated by the monooxygenase FMO1 and the Nudix hydrolase NUDT7." Bartsch M., Gobbato E., Bednarek P., Debey S., Schultze J.L., Bautor J., Parker J.E. Plant Cell 18:1038-1051(2006) [PubMed: 16531493] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[8]	"Analysis of Arabidopsis growth factor gene 1 (GFG1) encoding a nudix hydrolase during oxidative signaling." Jambunathan N., Mahalingam R. Planta 224:1-11(2006) [PubMed: 16328543] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION, BIOPHYSICOCHEMICAL PROPERTIES, DISRUPTION PHENOTYPE.
[9]	"AtNUDT7, a negative regulator of basal immunity in Arabidopsis, modulates two distinct defense response pathways and is involved in maintaining redox homeostasis." Ge X., Li G.-J., Wang S.-B., Zhu H., Zhu T., Wang X., Xia Y. Plant Physiol. 145:204-215(2007) [PubMed: 17660350] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF GLU-154, INDUCTION, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL049640 Genomic DNA. Translation: CAB40989.1. AL161534 Genomic DNA. Translation: CAB78314.1. CP002687 Genomic DNA. Translation: AEE83167.1. CP002687 Genomic DNA. Translation: AEE83168.1. CP002687 Genomic DNA. Translation: AEE83169.1. AF325104 mRNA. Translation: AAK17172.1. AF370209 mRNA. Translation: AAK44024.1. AY056344 mRNA. Translation: AAL07193.1. AY085375 mRNA. Translation: AAM62604.1.
IPI	IPI00537463.
PIR	T06630.
RefSeq	NP_193008.1. NM_117341.4. NP_849367.1. NM_179036.3. NP_849368.1. NM_179037.3.
UniGene	At.23449.
3D structure databases
ProteinModelPortal	Q9SU14.
SMR	Q9SU14. Positions 9-91, 103-264.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9SU14.
Proteomic databases
PRIDE	Q9SU14.
ProMEX	Q9SU14.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT4G12720.1; AT4G12720.1; AT4G12720. AT4G12720.2; AT4G12720.2; AT4G12720. AT4G12720.3; AT4G12720.3; AT4G12720.
GeneID	826884.
GenomeReviews	Gene locus AT4G12720 in contig CT486007_GR.
KEGG	ath:AT4G12720.
NMPDR	fig|3702.1.peg.18947.
Organism-specific databases
TAIR	At4g12720.
Phylogenomic databases
eggNOG	KOG0648.
GeneTree	EPGT00050000010426.
HOGENOM	HBG593794.
InParanoid	Q9SU14.
OMA	FMANICQ.
PhylomeDB	Q9SU14.
ProtClustDB	CLSN2683982.
Enzyme and pathway databases
BioCyc	ARA:AT4G12720-MONOMER.
Gene expression databases
Genevestigator	Q9SU14.
GermOnline	AT4G12720. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR003293. Nudix_hydrolase6-like. IPR020084. NUDIX_hydrolase_CS. IPR000086. NUDIX_hydrolase_dom. IPR015797. NUDIX_hydrolase_dom-like. [Graphical view]
Gene3D	G3DSA:3.90.79.10. NUDIX_hydrolase. 1 hit.
Pfam	PF00293. NUDIX. 1 hit. [Graphical view]
PRINTS	PR01356. GFGPROTEIN.
SUPFAM	SSF55811. NUDIX_hydrolase. 1 hit.
PROSITE	PS51462. NUDIX. 1 hit. PS00893. NUDIX_BOX. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUDT7_ARATH

Accession

Primary (citable) accession number: Q9SU14
Secondary accession number(s): Q8LEK5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	May 1, 2000
Last modified:	October 19, 2011
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program