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Protein

Nudix hydrolase 7

Gene

NUDT7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the hydrolysis of some nucleoside diphosphate derivatives. Can use both NADH and ADP-ribose as substrates, but not 8-oxo-dGTP, cyclic ADP-ribose, GDP-manose, UDP-glucose, ATP, or GTP. Exerts negative control of EDS1 signaling.5 Publications

Catalytic activityi

ADP-D-ribose + H2O = AMP + D-ribose 5-phosphate.
NAD(H) + H2O = AMP + NMN(H).

Cofactori

Enzyme regulationi

Not inhibited by fluoride.

Kineticsi

  1. KM=26.23 µM for NADH2 Publications
  2. KM=25.8 µM for ADP-ribose2 Publications
  1. Vmax=3.681 nmol/min/mg enzyme with NADH as substrate2 Publications
  2. Vmax=2678 nmol/min/mg enzyme with ADP-ribose as substrate2 Publications

pH dependencei

Optimum pH is 8.5.2 Publications

Temperature dependencei

Optimum temperature is 50 degrees Celsius.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi154MagnesiumBy similarity1
Metal bindingi158MagnesiumBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, NAD

Enzyme and pathway databases

BioCyciARA:AT4G12720-MONOMER.
BRENDAi3.6.1.13. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Nudix hydrolase 7 (EC:3.6.1.-)
Short name:
AtNUDT7
Alternative name(s):
ADP-ribose pyrophosphatase (EC:3.6.1.13)
NADH pyrophosphatase (EC:3.6.1.22)
Protein GROWTH FACTOR GENE 1
Gene namesi
Name:NUDT7
Synonyms:GFG1, NUDX7
Ordered Locus Names:At4g12720
ORF Names:T20K18.70
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G12720.

Subcellular locationi

  • Nucleus 1 Publication
  • Cytoplasm 1 Publication
  • Cell membrane 1 Publication

  • Note: Localized at the plasma membrane when in complex with GG2, but present in the cytoplasm when associated with GG1. Detected in the cytoplasm and nucleus when interacting with RACK1A.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleus Source: UniProtKB
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Disruption phenotypei

Growth retardation, constitutive pathogen resistance phenotype and increased levels of reactive oxygen species and NADH.3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi154E → Q: Loss of hydrolase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000571271 – 282Nudix hydrolase 7Add BLAST282

Expressioni

Tissue specificityi

Expressed in stems, leaves, roots, flowers and siliques.2 Publications

Inductioni

Rapid and transient induction by biotic and abiotic stresses. Not induced by H2O2.2 Publications

Gene expression databases

ExpressionAtlasiQ9SU14. baseline and differential.
GenevisibleiQ9SU14. AT.

Interactioni

Subunit structurei

Homodimer. Interacts with RACK1A, GG1 and GG2.2 Publications

Protein-protein interaction databases

BioGridi12181. 9 interactors.

Structurei

Secondary structure

1282
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi21 – 24Combined sources4
Helixi31 – 47Combined sources17
Beta strandi52 – 58Combined sources7
Helixi59 – 64Combined sources6
Helixi65 – 70Combined sources6
Beta strandi74 – 79Combined sources6
Beta strandi82 – 88Combined sources7
Beta strandi90 – 92Combined sources3
Beta strandi102 – 112Combined sources11
Turni113 – 116Combined sources4
Beta strandi117 – 126Combined sources10
Turni127 – 132Combined sources6
Beta strandi138 – 140Combined sources3
Helixi147 – 159Combined sources13
Beta strandi163 – 174Combined sources12
Beta strandi183 – 194Combined sources12
Beta strandi202 – 212Combined sources11
Helixi213 – 218Combined sources6
Helixi220 – 223Combined sources4
Helixi226 – 239Combined sources14
Beta strandi246 – 252Combined sources7
Beta strandi254 – 256Combined sources3
Beta strandi258 – 263Combined sources6
Helixi265 – 273Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4ZB3X-ray2.30A1-282[»]
4ZBPX-ray2.60A/B/C1-282[»]
ProteinModelPortaliQ9SU14.
SMRiQ9SU14.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini101 – 233Nudix hydrolasePROSITE-ProRule annotationAdd BLAST133

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi139 – 160Nudix boxAdd BLAST22

Sequence similaritiesi

Belongs to the Nudix hydrolase family.Curated
Contains 1 nudix hydrolase domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000240943.
InParanoidiQ9SU14.
PhylomeDBiQ9SU14.

Family and domain databases

Gene3Di3.90.79.10. 1 hit.
InterProiIPR003293. Nudix_hydrolase6-like.
IPR020084. NUDIX_hydrolase_CS.
IPR000086. NUDIX_hydrolase_dom.
IPR015797. NUDIX_hydrolase_dom-like.
[Graphical view]
PfamiPF00293. NUDIX. 1 hit.
[Graphical view]
PRINTSiPR01356. GFGPROTEIN.
SUPFAMiSSF55811. SSF55811. 1 hit.
PROSITEiPS51462. NUDIX. 1 hit.
PS00893. NUDIX_BOX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9SU14-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGTRAQQIPL LEGETDNYDG VTVTMVEPMD SEVFTESLRA SLSHWREEGK
60 70 80 90 100
KGIWIKLPLG LANLVEAAVS EGFRYHHAEP EYLMLVSWIS ETPDTIPANA
110 120 130 140 150
SHVVGAGALV INKNTKEVLV VQERSGFFKD KNVWKLPTGV INEGEDIWTG
160 170 180 190 200
VAREVEEETG IIADFVEVLA FRQSHKAILK KKTDMFFLCV LSPRSYDITE
210 220 230 240 250
QKSEILQAKW MPIQEYVDQP WNKKNEMFKF MANICQKKCE EEYLGFAIVP
260 270 280
TTTSSGKESF IYCNADHAKR LKVSRDQASA SL
Length:282
Mass (Da):31,884
Last modified:May 1, 2000 - v1
Checksum:iEA37FC0F1C2FDCB7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6Q → L in AAM62604 (Ref. 4) Curated1
Sequence conflicti217V → I in AAM62604 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL049640 Genomic DNA. Translation: CAB40989.1.
AL161534 Genomic DNA. Translation: CAB78314.1.
CP002687 Genomic DNA. Translation: AEE83167.1.
CP002687 Genomic DNA. Translation: AEE83168.1.
CP002687 Genomic DNA. Translation: AEE83169.1.
AF325104 mRNA. Translation: AAK17172.1.
AF370209 mRNA. Translation: AAK44024.1.
AY056344 mRNA. Translation: AAL07193.1.
AY085375 mRNA. Translation: AAM62604.1.
PIRiT06630.
RefSeqiNP_193008.1. NM_117341.5. [Q9SU14-1]
NP_849367.1. NM_179036.4. [Q9SU14-1]
NP_849368.1. NM_179037.3. [Q9SU14-1]
UniGeneiAt.23449.

Genome annotation databases

EnsemblPlantsiAT4G12720.1; AT4G12720.1; AT4G12720. [Q9SU14-1]
AT4G12720.2; AT4G12720.2; AT4G12720. [Q9SU14-1]
AT4G12720.3; AT4G12720.3; AT4G12720. [Q9SU14-1]
GeneIDi826884.
GrameneiAT4G12720.1; AT4G12720.1; AT4G12720.
AT4G12720.2; AT4G12720.2; AT4G12720.
AT4G12720.3; AT4G12720.3; AT4G12720.
KEGGiath:AT4G12720.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL049640 Genomic DNA. Translation: CAB40989.1.
AL161534 Genomic DNA. Translation: CAB78314.1.
CP002687 Genomic DNA. Translation: AEE83167.1.
CP002687 Genomic DNA. Translation: AEE83168.1.
CP002687 Genomic DNA. Translation: AEE83169.1.
AF325104 mRNA. Translation: AAK17172.1.
AF370209 mRNA. Translation: AAK44024.1.
AY056344 mRNA. Translation: AAL07193.1.
AY085375 mRNA. Translation: AAM62604.1.
PIRiT06630.
RefSeqiNP_193008.1. NM_117341.5. [Q9SU14-1]
NP_849367.1. NM_179036.4. [Q9SU14-1]
NP_849368.1. NM_179037.3. [Q9SU14-1]
UniGeneiAt.23449.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4ZB3X-ray2.30A1-282[»]
4ZBPX-ray2.60A/B/C1-282[»]
ProteinModelPortaliQ9SU14.
SMRiQ9SU14.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi12181. 9 interactors.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G12720.1; AT4G12720.1; AT4G12720. [Q9SU14-1]
AT4G12720.2; AT4G12720.2; AT4G12720. [Q9SU14-1]
AT4G12720.3; AT4G12720.3; AT4G12720. [Q9SU14-1]
GeneIDi826884.
GrameneiAT4G12720.1; AT4G12720.1; AT4G12720.
AT4G12720.2; AT4G12720.2; AT4G12720.
AT4G12720.3; AT4G12720.3; AT4G12720.
KEGGiath:AT4G12720.

Organism-specific databases

TAIRiAT4G12720.

Phylogenomic databases

HOGENOMiHOG000240943.
InParanoidiQ9SU14.
PhylomeDBiQ9SU14.

Enzyme and pathway databases

BioCyciARA:AT4G12720-MONOMER.
BRENDAi3.6.1.13. 399.

Miscellaneous databases

PROiQ9SU14.

Gene expression databases

ExpressionAtlasiQ9SU14. baseline and differential.
GenevisibleiQ9SU14. AT.

Family and domain databases

Gene3Di3.90.79.10. 1 hit.
InterProiIPR003293. Nudix_hydrolase6-like.
IPR020084. NUDIX_hydrolase_CS.
IPR000086. NUDIX_hydrolase_dom.
IPR015797. NUDIX_hydrolase_dom-like.
[Graphical view]
PfamiPF00293. NUDIX. 1 hit.
[Graphical view]
PRINTSiPR01356. GFGPROTEIN.
SUPFAMiSSF55811. SSF55811. 1 hit.
PROSITEiPS51462. NUDIX. 1 hit.
PS00893. NUDIX_BOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUDT7_ARATH
AccessioniPrimary (citable) accession number: Q9SU14
Secondary accession number(s): Q8LEK5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.