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Q9STZ3

- PLCD8_ARATH

UniProt

Q9STZ3 - PLCD8_ARATH

Protein

Phosphoinositide phospholipase C 8

Gene

PLC8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.By similarity

    Catalytic activityi

    1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

    Cofactori

    Calcium.

    GO - Molecular functioni

    1. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
    2. signal transducer activity Source: UniProtKB-KW

    GO - Biological processi

    1. intracellular signal transduction Source: InterPro
    2. lipid catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Transducer

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Enzyme and pathway databases

    BioCyciARA:AT3G47290-MONOMER.
    ReactomeiREACT_187614. Synthesis of IP3 and IP4 in the cytosol.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoinositide phospholipase C 8 (EC:3.1.4.11)
    Alternative name(s):
    Phosphoinositide phospholipase PLC8
    Short name:
    AtPLC8
    Short name:
    PI-PLC8
    Gene namesi
    Name:PLC8
    Synonyms:PLC2
    Ordered Locus Names:At3g47290
    ORF Names:T21L8.40
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G47290.

    Subcellular locationi

    Cell membrane By similarity; Peripheral membrane protein By similarity

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 531531Phosphoinositide phospholipase C 8PRO_0000324133Add
    BLAST

    Expressioni

    Tissue specificityi

    Expressed in leaves, roots, flowers and siliques.1 Publication

    Gene expression databases

    GenevestigatoriQ9STZ3.

    Interactioni

    Protein-protein interaction databases

    BioGridi9202. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9STZ3.
    SMRiQ9STZ3. Positions 36-527.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini105 – 247143PI-PLC X-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini267 – 385119PI-PLC Y-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini390 – 496107C2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 C2 domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG149692.
    HOGENOMiHOG000244119.
    InParanoidiQ9STZ3.
    OMAiEAGAIND.
    PhylomeDBiQ9STZ3.

    Family and domain databases

    Gene3Di2.60.40.150. 1 hit.
    3.20.20.190. 1 hit.
    InterProiIPR000008. C2_dom.
    IPR001192. PI-PLC_fam.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view]
    PANTHERiPTHR10336. PTHR10336. 1 hit.
    PfamiPF00168. C2. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view]
    PRINTSiPR00390. PHPHLIPASEC.
    SMARTiSM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEiPS50004. C2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9STZ3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLVTRRWESH PANSPDLILQ FFGNEFHGYG DDMPETLRRL TELLGYEKEE    50
    DGAGMNAAKK IAAELNRRKD DIPAFRRLRC LELDQLNEFL FSTKLNPPIG 100
    DQVHHDMHAP LSHYFIHTSL NSYFTGNVFG KYSILPIIEA LEQGVRVVEL 150
    DLWPDGRGSI CVRPSWNFEK PLKLQECLDS IKEHAFTKCT YPLIITFKDG 200
    LKPELQSKAT QMIQQTFNHM VYHHDPHSLE VFPSPQQLRN KILISRRPPK 250
    ELLYANDDDG KVGVRNGVEI RQHPADPNYQ SLVSFHVVEP RGMLQNVLTG 300
    KANKIQRPGW YETDIISFTQ KRFLRTRPQR KLLIYAPYKP QRAWMHGAQL 350
    IALSRKEEKE KLWLMQGMFR ANGGCGYVKK PDFLLNAGPS GVFYPTVNPV 400
    VVKILKVKIY MGDGWIVDFK KRIGRLSKPD LYVRISIAGV PHDENIMKTT 450
    VKNNEWTPTW GEEFTFPLTY PDLALISFEV YDYEVSTADA FCGQTCLPVS 500
    ELIEGIRAVP LYDERGKACS STMLLTRFKW S 531
    Length:531
    Mass (Da):61,076
    Last modified:May 1, 2000 - v1
    Checksum:iBA4C2C960029771F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti82 – 821E → K in CAA59962. 1 PublicationCurated
    Sequence conflicti358 – 3581E → D in CAA59962. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X85973 mRNA. Translation: CAA59962.1.
    AL096860 Genomic DNA. Translation: CAB51201.1.
    CP002686 Genomic DNA. Translation: AEE78261.1.
    BT004182 mRNA. Translation: AAO42201.1.
    BT005470 mRNA. Translation: AAO63890.1.
    PIRiT12984. S54098.
    RefSeqiNP_190313.1. NM_114596.3.
    UniGeneiAt.47563.

    Genome annotation databases

    EnsemblPlantsiAT3G47290.1; AT3G47290.1; AT3G47290.
    GeneIDi823882.
    KEGGiath:AT3G47290.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X85973 mRNA. Translation: CAA59962.1 .
    AL096860 Genomic DNA. Translation: CAB51201.1 .
    CP002686 Genomic DNA. Translation: AEE78261.1 .
    BT004182 mRNA. Translation: AAO42201.1 .
    BT005470 mRNA. Translation: AAO63890.1 .
    PIRi T12984. S54098.
    RefSeqi NP_190313.1. NM_114596.3.
    UniGenei At.47563.

    3D structure databases

    ProteinModelPortali Q9STZ3.
    SMRi Q9STZ3. Positions 36-527.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 9202. 1 interaction.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G47290.1 ; AT3G47290.1 ; AT3G47290 .
    GeneIDi 823882.
    KEGGi ath:AT3G47290.

    Organism-specific databases

    TAIRi AT3G47290.

    Phylogenomic databases

    eggNOGi NOG149692.
    HOGENOMi HOG000244119.
    InParanoidi Q9STZ3.
    OMAi EAGAIND.
    PhylomeDBi Q9STZ3.

    Enzyme and pathway databases

    BioCyci ARA:AT3G47290-MONOMER.
    Reactomei REACT_187614. Synthesis of IP3 and IP4 in the cytosol.

    Gene expression databases

    Genevestigatori Q9STZ3.

    Family and domain databases

    Gene3Di 2.60.40.150. 1 hit.
    3.20.20.190. 1 hit.
    InterProi IPR000008. C2_dom.
    IPR001192. PI-PLC_fam.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view ]
    PANTHERi PTHR10336. PTHR10336. 1 hit.
    Pfami PF00168. C2. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view ]
    PRINTSi PR00390. PHPHLIPASEC.
    SMARTi SM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEi PS50004. C2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Kopka J., Willmitzer L., Mueller-Roeber B.
      Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. C24.
    2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C."
      Mueller-Roeber B., Pical C.
      Plant Physiol. 130:22-46(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    6. "Gene-specific expression and calcium activation of Arabidopsis thaliana phospholipase C isoforms."
      Hunt L., Otterhag L., Lee J.C., Lasheen T., Hunt J., Seki M., Shinozaki K., Sommarin M., Gilmour D.J., Pical C., Gray J.E.
      New Phytol. 162:643-654(2004)
      [AGRICOLA] [Europe PMC]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiPLCD8_ARATH
    AccessioniPrimary (citable) accession number: Q9STZ3
    Secondary accession number(s): Q42582
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Caution

    Contains substitutions of several amino acids thought to be essential for catalytic activity. Its enzyme activity is therefore unsure.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3