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Q9STZ3

- PLCD8_ARATH

UniProt

Q9STZ3 - PLCD8_ARATH

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Protein
Phosphoinositide phospholipase C 8
Gene
PLC8, PLC2, At3g47290, T21L8.40
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes By similarity.

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

Calcium.

GO - Molecular functioni

  1. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
  2. signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  1. intracellular signal transduction Source: InterPro
  2. lipid catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transducer

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciARA:AT3G47290-MONOMER.
ReactomeiREACT_187614. Synthesis of IP3 and IP4 in the cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoinositide phospholipase C 8 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase PLC8
Short name:
AtPLC8
Short name:
PI-PLC8
Gene namesi
Name:PLC8
Synonyms:PLC2
Ordered Locus Names:At3g47290
ORF Names:T21L8.40
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G47290.

Subcellular locationi

GO - Cellular componenti

  1. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 531531Phosphoinositide phospholipase C 8
PRO_0000324133Add
BLAST

Expressioni

Tissue specificityi

Expressed in leaves, roots, flowers and siliques.1 Publication

Gene expression databases

GenevestigatoriQ9STZ3.

Interactioni

Protein-protein interaction databases

BioGridi9202. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9STZ3.
SMRiQ9STZ3. Positions 36-527.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini105 – 247143PI-PLC X-box
Add
BLAST
Domaini267 – 385119PI-PLC Y-box
Add
BLAST
Domaini390 – 496107C2
Add
BLAST

Sequence similaritiesi

Contains 1 C2 domain.

Phylogenomic databases

eggNOGiNOG149692.
HOGENOMiHOG000244119.
InParanoidiQ9STZ3.
OMAiEAGAIND.
PhylomeDBiQ9STZ3.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
3.20.20.190. 1 hit.
InterProiIPR000008. C2_dom.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PfamiPF00168. C2. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9STZ3-1 [UniParc]FASTAAdd to Basket

« Hide

MLVTRRWESH PANSPDLILQ FFGNEFHGYG DDMPETLRRL TELLGYEKEE    50
DGAGMNAAKK IAAELNRRKD DIPAFRRLRC LELDQLNEFL FSTKLNPPIG 100
DQVHHDMHAP LSHYFIHTSL NSYFTGNVFG KYSILPIIEA LEQGVRVVEL 150
DLWPDGRGSI CVRPSWNFEK PLKLQECLDS IKEHAFTKCT YPLIITFKDG 200
LKPELQSKAT QMIQQTFNHM VYHHDPHSLE VFPSPQQLRN KILISRRPPK 250
ELLYANDDDG KVGVRNGVEI RQHPADPNYQ SLVSFHVVEP RGMLQNVLTG 300
KANKIQRPGW YETDIISFTQ KRFLRTRPQR KLLIYAPYKP QRAWMHGAQL 350
IALSRKEEKE KLWLMQGMFR ANGGCGYVKK PDFLLNAGPS GVFYPTVNPV 400
VVKILKVKIY MGDGWIVDFK KRIGRLSKPD LYVRISIAGV PHDENIMKTT 450
VKNNEWTPTW GEEFTFPLTY PDLALISFEV YDYEVSTADA FCGQTCLPVS 500
ELIEGIRAVP LYDERGKACS STMLLTRFKW S 531
Length:531
Mass (Da):61,076
Last modified:May 1, 2000 - v1
Checksum:iBA4C2C960029771F
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti82 – 821E → K in CAA59962. 1 Publication
Sequence conflicti358 – 3581E → D in CAA59962. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X85973 mRNA. Translation: CAA59962.1.
AL096860 Genomic DNA. Translation: CAB51201.1.
CP002686 Genomic DNA. Translation: AEE78261.1.
BT004182 mRNA. Translation: AAO42201.1.
BT005470 mRNA. Translation: AAO63890.1.
PIRiT12984. S54098.
RefSeqiNP_190313.1. NM_114596.3.
UniGeneiAt.47563.

Genome annotation databases

EnsemblPlantsiAT3G47290.1; AT3G47290.1; AT3G47290.
GeneIDi823882.
KEGGiath:AT3G47290.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X85973 mRNA. Translation: CAA59962.1 .
AL096860 Genomic DNA. Translation: CAB51201.1 .
CP002686 Genomic DNA. Translation: AEE78261.1 .
BT004182 mRNA. Translation: AAO42201.1 .
BT005470 mRNA. Translation: AAO63890.1 .
PIRi T12984. S54098.
RefSeqi NP_190313.1. NM_114596.3.
UniGenei At.47563.

3D structure databases

ProteinModelPortali Q9STZ3.
SMRi Q9STZ3. Positions 36-527.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 9202. 1 interaction.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G47290.1 ; AT3G47290.1 ; AT3G47290 .
GeneIDi 823882.
KEGGi ath:AT3G47290.

Organism-specific databases

TAIRi AT3G47290.

Phylogenomic databases

eggNOGi NOG149692.
HOGENOMi HOG000244119.
InParanoidi Q9STZ3.
OMAi EAGAIND.
PhylomeDBi Q9STZ3.

Enzyme and pathway databases

BioCyci ARA:AT3G47290-MONOMER.
Reactomei REACT_187614. Synthesis of IP3 and IP4 in the cytosol.

Gene expression databases

Genevestigatori Q9STZ3.

Family and domain databases

Gene3Di 2.60.40.150. 1 hit.
3.20.20.190. 1 hit.
InterProi IPR000008. C2_dom.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view ]
PANTHERi PTHR10336. PTHR10336. 1 hit.
Pfami PF00168. C2. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view ]
PRINTSi PR00390. PHPHLIPASEC.
SMARTi SM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEi PS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Kopka J., Willmitzer L., Mueller-Roeber B.
    Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. C24.
  2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C."
    Mueller-Roeber B., Pical C.
    Plant Physiol. 130:22-46(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  6. "Gene-specific expression and calcium activation of Arabidopsis thaliana phospholipase C isoforms."
    Hunt L., Otterhag L., Lee J.C., Lasheen T., Hunt J., Seki M., Shinozaki K., Sommarin M., Gilmour D.J., Pical C., Gray J.E.
    New Phytol. 162:643-654(2004)
    [AGRICOLA] [Europe PMC]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiPLCD8_ARATH
AccessioniPrimary (citable) accession number: Q9STZ3
Secondary accession number(s): Q42582
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: May 1, 2000
Last modified: September 3, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

Contains substitutions of several amino acids thought to be essential for catalytic activity. Its enzyme activity is therefore unsure.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi