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Protein

Phosphoinositide phospholipase C 8

Gene

PLC8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.By similarity

Caution

Contains substitutions of several amino acids thought to be essential for catalytic activity. Its enzyme activity is therefore unsure.Curated

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Transducer
Biological processLipid degradation, Lipid metabolism

Enzyme and pathway databases

BRENDAi3.1.4.11 399
ReactomeiR-ATH-112043 PLC beta mediated events
R-ATH-114604 GPVI-mediated activation cascade
R-ATH-1855204 Synthesis of IP3 and IP4 in the cytosol
R-ATH-202433 Generation of second messenger molecules
R-ATH-399997 Acetylcholine regulates insulin secretion
R-ATH-416476 G alpha (q) signalling events
R-ATH-434316 Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion
R-ATH-5607764 CLEC7A (Dectin-1) signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoinositide phospholipase C 8 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase PLC8
Short name:
AtPLC8
Short name:
PI-PLC8
Gene namesi
Name:PLC8
Synonyms:PLC2
Ordered Locus Names:At3g47290
ORF Names:T21L8.40
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G47290
TAIRilocus:2099530 AT3G47290

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003241331 – 531Phosphoinositide phospholipase C 8Add BLAST531

Proteomic databases

PaxDbiQ9STZ3

Expressioni

Tissue specificityi

Expressed in leaves, roots, flowers and siliques.1 Publication

Gene expression databases

ExpressionAtlasiQ9STZ3 baseline and differential
GenevisibleiQ9STZ3 AT

Interactioni

Protein-protein interaction databases

BioGridi9202, 1 interactor
STRINGi3702.AT3G47290.1

Structurei

3D structure databases

ProteinModelPortaliQ9STZ3
SMRiQ9STZ3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini105 – 247PI-PLC X-boxPROSITE-ProRule annotationAdd BLAST143
Domaini267 – 385PI-PLC Y-boxPROSITE-ProRule annotationAdd BLAST119
Domaini390 – 496C2PROSITE-ProRule annotationAdd BLAST107

Phylogenomic databases

eggNOGiKOG0169 Eukaryota
ENOG410XPSW LUCA
HOGENOMiHOG000244119
InParanoidiQ9STZ3
OrthoDBiEOG0936050V
PhylomeDBiQ9STZ3

Family and domain databases

Gene3Di2.60.40.150, 1 hit
3.20.20.190, 1 hit
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR001192 PI-PLC_fam
IPR017946 PLC-like_Pdiesterase_TIM-brl
IPR000909 PLipase_C_PInositol-sp_X_dom
IPR001711 PLipase_C_Pinositol-sp_Y
PANTHERiPTHR10336 PTHR10336, 2 hits
PfamiView protein in Pfam
PF00168 C2, 1 hit
PF00388 PI-PLC-X, 1 hit
PF00387 PI-PLC-Y, 1 hit
PRINTSiPR00390 PHPHLIPASEC
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00148 PLCXc, 1 hit
SM00149 PLCYc, 1 hit
SUPFAMiSSF51695 SSF51695, 1 hit
PROSITEiView protein in PROSITE
PS50004 C2, 1 hit
PS50007 PIPLC_X_DOMAIN, 1 hit
PS50008 PIPLC_Y_DOMAIN, 1 hit

Sequencei

Sequence statusi: Complete.

Q9STZ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVTRRWESH PANSPDLILQ FFGNEFHGYG DDMPETLRRL TELLGYEKEE
60 70 80 90 100
DGAGMNAAKK IAAELNRRKD DIPAFRRLRC LELDQLNEFL FSTKLNPPIG
110 120 130 140 150
DQVHHDMHAP LSHYFIHTSL NSYFTGNVFG KYSILPIIEA LEQGVRVVEL
160 170 180 190 200
DLWPDGRGSI CVRPSWNFEK PLKLQECLDS IKEHAFTKCT YPLIITFKDG
210 220 230 240 250
LKPELQSKAT QMIQQTFNHM VYHHDPHSLE VFPSPQQLRN KILISRRPPK
260 270 280 290 300
ELLYANDDDG KVGVRNGVEI RQHPADPNYQ SLVSFHVVEP RGMLQNVLTG
310 320 330 340 350
KANKIQRPGW YETDIISFTQ KRFLRTRPQR KLLIYAPYKP QRAWMHGAQL
360 370 380 390 400
IALSRKEEKE KLWLMQGMFR ANGGCGYVKK PDFLLNAGPS GVFYPTVNPV
410 420 430 440 450
VVKILKVKIY MGDGWIVDFK KRIGRLSKPD LYVRISIAGV PHDENIMKTT
460 470 480 490 500
VKNNEWTPTW GEEFTFPLTY PDLALISFEV YDYEVSTADA FCGQTCLPVS
510 520 530
ELIEGIRAVP LYDERGKACS STMLLTRFKW S
Length:531
Mass (Da):61,076
Last modified:May 1, 2000 - v1
Checksum:iBA4C2C960029771F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti82E → K in CAA59962 (Ref. 1) Curated1
Sequence conflicti358E → D in CAA59962 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85973 mRNA Translation: CAA59962.1
AL096860 Genomic DNA Translation: CAB51201.1
CP002686 Genomic DNA Translation: AEE78261.1
BT004182 mRNA Translation: AAO42201.1
BT005470 mRNA Translation: AAO63890.1
PIRiT12984 S54098
RefSeqiNP_190313.1, NM_114596.4
UniGeneiAt.47563

Genome annotation databases

EnsemblPlantsiAT3G47290.1; AT3G47290.1; AT3G47290
GeneIDi823882
GrameneiAT3G47290.1; AT3G47290.1; AT3G47290
KEGGiath:AT3G47290

Similar proteinsi

Entry informationi

Entry nameiPLCD8_ARATH
AccessioniPrimary (citable) accession number: Q9STZ3
Secondary accession number(s): Q42582
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: May 1, 2000
Last modified: April 25, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health