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Protein

Phosphoinositide phospholipase C 8

Gene

PLC8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.By similarity

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Transducer
Biological processLipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciARA:AT3G47290-MONOMER.
BRENDAi3.1.4.11. 399.
ReactomeiR-ATH-112043. PLC beta mediated events.
R-ATH-114604. GPVI-mediated activation cascade.
R-ATH-1855204. Synthesis of IP3 and IP4 in the cytosol.
R-ATH-202433. Generation of second messenger molecules.
R-ATH-399997. Acetylcholine regulates insulin secretion.
R-ATH-416476. G alpha (q) signalling events.
R-ATH-434316. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.
R-ATH-5607764. CLEC7A (Dectin-1) signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoinositide phospholipase C 8 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase PLC8
Short name:
AtPLC8
Short name:
PI-PLC8
Gene namesi
Name:PLC8
Synonyms:PLC2
Ordered Locus Names:At3g47290
ORF Names:T21L8.40
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G47290.
TAIRilocus:2099530. AT3G47290.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003241331 – 531Phosphoinositide phospholipase C 8Add BLAST531

Proteomic databases

PaxDbiQ9STZ3.

Expressioni

Tissue specificityi

Expressed in leaves, roots, flowers and siliques.1 Publication

Gene expression databases

ExpressionAtlasiQ9STZ3. baseline and differential.
GenevisibleiQ9STZ3. AT.

Interactioni

Protein-protein interaction databases

BioGridi9202. 1 interactor.
STRINGi3702.AT3G47290.1.

Structurei

3D structure databases

ProteinModelPortaliQ9STZ3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini105 – 247PI-PLC X-boxPROSITE-ProRule annotationAdd BLAST143
Domaini267 – 385PI-PLC Y-boxPROSITE-ProRule annotationAdd BLAST119
Domaini390 – 496C2PROSITE-ProRule annotationAdd BLAST107

Phylogenomic databases

eggNOGiKOG0169. Eukaryota.
ENOG410XPSW. LUCA.
HOGENOMiHOG000244119.
InParanoidiQ9STZ3.
OrthoDBiEOG0936050V.
PhylomeDBiQ9STZ3.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiView protein in InterPro
IPR000008. C2_dom.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
PANTHERiPTHR10336. PTHR10336. 1 hit.
PfamiView protein in Pfam
PF00168. C2. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
PRINTSiPR00390. PHPHLIPASEC.
SMARTiView protein in SMART
SM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiView protein in PROSITE
PS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9STZ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVTRRWESH PANSPDLILQ FFGNEFHGYG DDMPETLRRL TELLGYEKEE
60 70 80 90 100
DGAGMNAAKK IAAELNRRKD DIPAFRRLRC LELDQLNEFL FSTKLNPPIG
110 120 130 140 150
DQVHHDMHAP LSHYFIHTSL NSYFTGNVFG KYSILPIIEA LEQGVRVVEL
160 170 180 190 200
DLWPDGRGSI CVRPSWNFEK PLKLQECLDS IKEHAFTKCT YPLIITFKDG
210 220 230 240 250
LKPELQSKAT QMIQQTFNHM VYHHDPHSLE VFPSPQQLRN KILISRRPPK
260 270 280 290 300
ELLYANDDDG KVGVRNGVEI RQHPADPNYQ SLVSFHVVEP RGMLQNVLTG
310 320 330 340 350
KANKIQRPGW YETDIISFTQ KRFLRTRPQR KLLIYAPYKP QRAWMHGAQL
360 370 380 390 400
IALSRKEEKE KLWLMQGMFR ANGGCGYVKK PDFLLNAGPS GVFYPTVNPV
410 420 430 440 450
VVKILKVKIY MGDGWIVDFK KRIGRLSKPD LYVRISIAGV PHDENIMKTT
460 470 480 490 500
VKNNEWTPTW GEEFTFPLTY PDLALISFEV YDYEVSTADA FCGQTCLPVS
510 520 530
ELIEGIRAVP LYDERGKACS STMLLTRFKW S
Length:531
Mass (Da):61,076
Last modified:May 1, 2000 - v1
Checksum:iBA4C2C960029771F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti82E → K in CAA59962 (Ref. 1) Curated1
Sequence conflicti358E → D in CAA59962 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85973 mRNA. Translation: CAA59962.1.
AL096860 Genomic DNA. Translation: CAB51201.1.
CP002686 Genomic DNA. Translation: AEE78261.1.
BT004182 mRNA. Translation: AAO42201.1.
BT005470 mRNA. Translation: AAO63890.1.
PIRiT12984. S54098.
RefSeqiNP_190313.1. NM_114596.4.
UniGeneiAt.47563.

Genome annotation databases

EnsemblPlantsiAT3G47290.1; AT3G47290.1; AT3G47290.
GeneIDi823882.
GrameneiAT3G47290.1; AT3G47290.1; AT3G47290.
KEGGiath:AT3G47290.

Similar proteinsi

Entry informationi

Entry nameiPLCD8_ARATH
AccessioniPrimary (citable) accession number: Q9STZ3
Secondary accession number(s): Q42582
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: May 1, 2000
Last modified: August 30, 2017
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

Contains substitutions of several amino acids thought to be essential for catalytic activity. Its enzyme activity is therefore unsure.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names