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Q9STX5

- ENPL_ARATH

UniProt

Q9STX5 - ENPL_ARATH

Protein

Endoplasmin homolog

Gene

SHD

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    May have a molecular chaperone role in the processing of secreted materials. Required for shoot apical meristem (SAM), root apical meristem (RAM) and floral meristem (FM) formation, probably by regulating the folding of CLAVATA proteins (CLVs). Also involved in pollen tube elongation.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei110 – 1101ATPBy similarity
    Binding sitei154 – 1541ATPBy similarity
    Binding sitei167 – 1671ATPBy similarity
    Binding sitei173 – 1731ATPBy similarity
    Binding sitei199 – 1991ATP; via amide nitrogenBy similarity
    Binding sitei455 – 4551ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein folding Source: InterPro
    2. protein secretion Source: TAIR
    3. regulation of meristem growth Source: TAIR
    4. regulation of meristem structural organization Source: TAIR
    5. response to cadmium ion Source: TAIR
    6. response to cold Source: TAIR
    7. response to salt stress Source: TAIR
    8. response to water deprivation Source: TAIR

    Keywords - Molecular functioni

    Chaperone

    Keywords - Ligandi

    ATP-binding, Calcium, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_108190. ATF6-alpha activates chaperone genes.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoplasmin homolog
    Alternative name(s):
    Glucose-regulated protein 94 homolog
    Short name:
    GRP-94 homolog
    HSP90-like protein 7
    Protein SHEPHERD
    Gene namesi
    Name:SHD
    Synonyms:HSP90-7
    Ordered Locus Names:At4g24190
    ORF Names:T19F6.1,T22A6.20
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G24190.

    Subcellular locationi

    GO - Cellular componenti

    1. apoplast Source: TAIR
    2. chloroplast Source: TAIR
    3. endoplasmic reticulum Source: TAIR
    4. endoplasmic reticulum lumen Source: UniProtKB-SubCell
    5. membrane Source: TAIR
    6. mitochondrion Source: TAIR
    7. nucleus Source: TAIR
    8. plasma membrane Source: TAIR
    9. plasmodesma Source: TAIR
    10. vacuolar membrane Source: TAIR
    11. vacuole Source: TAIR

    Keywords - Cellular componenti

    Endoplasmic reticulum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 823800Endoplasmin homologPRO_0000226071Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi110 – 1101N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi452 – 4521N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi620 – 6201N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ9STX5.
    PRIDEiQ9STX5.

    Expressioni

    Tissue specificityi

    Ubiquitous.1 Publication

    Inductioni

    Seems inhibited by heat shock.1 Publication

    Gene expression databases

    ArrayExpressiQ9STX5.
    GenevestigatoriQ9STX5.

    Interactioni

    Subunit structurei

    Interacts with FKBP42.1 Publication

    Protein-protein interaction databases

    BioGridi13809. 1 interaction.
    IntActiQ9STX5. 9 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9STX5.
    SMRiQ9STX5. Positions 75-776.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi820 – 8234Prevents secretion from ERBy similarity

    Sequence similaritiesi

    Belongs to the heat shock protein 90 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0326.
    HOGENOMiHOG000031988.
    InParanoidiQ9STX5.
    KOiK09487.
    OMAiQSSHHPS.
    PhylomeDBiQ9STX5.

    Family and domain databases

    Gene3Di3.30.565.10. 1 hit.
    HAMAPiMF_00505. HSP90.
    InterProiIPR003594. HATPase_ATP-bd.
    IPR019805. Heat_shock_protein_90_CS.
    IPR001404. Hsp90_fam.
    IPR020575. Hsp90_N.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    [Graphical view]
    PANTHERiPTHR11528. PTHR11528. 1 hit.
    PfamiPF00183. HSP90. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002583. Hsp90. 1 hit.
    PRINTSiPR00775. HEATSHOCK90.
    SMARTiSM00387. HATPase_c. 1 hit.
    [Graphical view]
    SUPFAMiSSF54211. SSF54211. 1 hit.
    SSF55874. SSF55874. 1 hit.
    PROSITEiPS00014. ER_TARGET. 1 hit.
    PS00298. HSP90. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q9STX5-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRKRTLVSVL FLFSLLFLLP DQGRKLHANA EESSDDVTDP PKVEEKIGGH    50
    GGLSTDSDVV HRESESMSKK TLRSNAEKFE FQAEVSRLMD IIINSLYSNK 100
    DIFLRELISN ASDALDKIRF LALTDKDVLG EGDTAKLEIQ IKLDKAKKIL 150
    SIRDRGIGMT KEDLIKNLGT IAKSGTSAFV EKMQSSGDLN LIGQFGVGFY 200
    SAYLVADYIE VISKHNDDSQ YVWESKANGK FAVSEDTWNE PLGRGTEIRL 250
    HLRDEAGEYL EESKLKELVK RYSEFINFPI SLWASKEVET EVPVEEDESA 300
    DEETETTSTE EEKEEDAEEE DGEKKQKTKK VKETVYEWEL LNDVKAIWLR 350
    SPKEVTEEEY TKFYHSLSKD FTDEKPMAWS HFNAEGDVEF KAVLYVPPKA 400
    PHDLYESYYN SNKANLKLYV RRVFISDEFD ELLPKYLSFL KGLVDSDTLP 450
    LNVSREMLQQ HSSLKTIKKK LIRKALDMIR KLAEEDPDEI HDDEKKDVEK 500
    SGENDEKKGQ YTKFWNEFGK SVKLGIIEDA ANRNRLAKLL RFETTKSDGK 550
    LTSLDQYIKR MKKSQKDIFY ITGSSKEQLE KSPFLERLIK KGYEVIFFTD 600
    PVDEYLMQYL MDYEDKKFQN VSKEGLKVGK DSKDKELKEA FKELTKWWKG 650
    NLASENVDDV KISNRLADTP CVVVTSKFGW SANMERIMQS QTLSDANKQA 700
    YMRGKRVLEI NPRHPIIKEL KDRIASDPED ESVKETAQLM YQTALIESGF 750
    ILTDPKDFAA RIYNSVKSGL NISPDAVADE EIEAAEEPET SEATETKSDD 800
    LAGGLNIEAE PVEQQEENTK DEL 823
    Length:823
    Mass (Da):94,204
    Last modified:May 1, 2000 - v1
    Checksum:i54C88E182A251990
    GO

    Sequence cautioni

    The sequence AAB63606.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB064528 mRNA. Translation: BAB86369.1.
    AC002343 Genomic DNA. Translation: AAB63606.1. Sequence problems.
    AL078637 Genomic DNA. Translation: CAB45054.1.
    AL161561 Genomic DNA. Translation: CAB79329.1.
    CP002687 Genomic DNA. Translation: AEE84861.1.
    AY039895 mRNA. Translation: AAK63999.1.
    AY072394 mRNA. Translation: AAL62386.1.
    BT004527 mRNA. Translation: AAO42773.1.
    AK221485 mRNA. Translation: BAD94659.1.
    PIRiT09882.
    RefSeqiNP_194150.1. NM_118552.3. [Q9STX5-1]
    UniGeneiAt.24111.

    Genome annotation databases

    EnsemblPlantsiAT4G24190.1; AT4G24190.1; AT4G24190. [Q9STX5-1]
    GeneIDi828520.
    KEGGiath:AT4G24190.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB064528 mRNA. Translation: BAB86369.1 .
    AC002343 Genomic DNA. Translation: AAB63606.1 . Sequence problems.
    AL078637 Genomic DNA. Translation: CAB45054.1 .
    AL161561 Genomic DNA. Translation: CAB79329.1 .
    CP002687 Genomic DNA. Translation: AEE84861.1 .
    AY039895 mRNA. Translation: AAK63999.1 .
    AY072394 mRNA. Translation: AAL62386.1 .
    BT004527 mRNA. Translation: AAO42773.1 .
    AK221485 mRNA. Translation: BAD94659.1 .
    PIRi T09882.
    RefSeqi NP_194150.1. NM_118552.3. [Q9STX5-1 ]
    UniGenei At.24111.

    3D structure databases

    ProteinModelPortali Q9STX5.
    SMRi Q9STX5. Positions 75-776.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 13809. 1 interaction.
    IntActi Q9STX5. 9 interactions.

    Proteomic databases

    PaxDbi Q9STX5.
    PRIDEi Q9STX5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G24190.1 ; AT4G24190.1 ; AT4G24190 . [Q9STX5-1 ]
    GeneIDi 828520.
    KEGGi ath:AT4G24190.

    Organism-specific databases

    TAIRi AT4G24190.

    Phylogenomic databases

    eggNOGi COG0326.
    HOGENOMi HOG000031988.
    InParanoidi Q9STX5.
    KOi K09487.
    OMAi QSSHHPS.
    PhylomeDBi Q9STX5.

    Enzyme and pathway databases

    Reactomei REACT_108190. ATF6-alpha activates chaperone genes.

    Miscellaneous databases

    PROi Q9STX5.

    Gene expression databases

    ArrayExpressi Q9STX5.
    Genevestigatori Q9STX5.

    Family and domain databases

    Gene3Di 3.30.565.10. 1 hit.
    HAMAPi MF_00505. HSP90.
    InterProi IPR003594. HATPase_ATP-bd.
    IPR019805. Heat_shock_protein_90_CS.
    IPR001404. Hsp90_fam.
    IPR020575. Hsp90_N.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    [Graphical view ]
    PANTHERi PTHR11528. PTHR11528. 1 hit.
    Pfami PF00183. HSP90. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002583. Hsp90. 1 hit.
    PRINTSi PR00775. HEATSHOCK90.
    SMARTi SM00387. HATPase_c. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54211. SSF54211. 1 hit.
    SSF55874. SSF55874. 1 hit.
    PROSITEi PS00014. ER_TARGET. 1 hit.
    PS00298. HSP90. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "SHEPHERD is the Arabidopsis GRP94 responsible for the formation of functional CLAVATA proteins."
      Ishiguro S., Watanabe Y., Ito N., Nonaka H., Takeda N., Sakai T., Kanaya H., Okada K.
      EMBO J. 21:898-908(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION.
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-238.
      Strain: cv. Columbia.
    6. "The Hsp90 family of proteins in Arabidopsis thaliana."
      Krishna P., Gloor G.
      Cell Stress Chaperones 6:238-246(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    7. "Characterization of Arabidopsis thaliana AtFKBP42 that is membrane-bound and interacts with Hsp90."
      Kamphausen T., Fanghaenel J., Neumann D., Schulz B., Rahfeld J.-U.
      Plant J. 32:263-276(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH FKBP42.

    Entry informationi

    Entry nameiENPL_ARATH
    AccessioniPrimary (citable) accession number: Q9STX5
    Secondary accession number(s): O22972, Q56Y38, Q8VY71
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 7, 2006
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3