Q9STX5 (ENPL_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoplasmin homolog

Alternative name(s):
Glucose-regulated protein 94 homolog
Short name=GRP-94 homolog
HSP90-like protein 7
Protein SHEPHERD

Gene names

Name:	SHD
Synonyms:	HSP90-7
Ordered Locus Names:	At4g24190
ORF Names:	T19F6.1,T22A6.20

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	823 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May have a molecular chaperone role in the processing of secreted materials. Required for shoot apical meristem (SAM), root apical meristem (RAM) and floral meristem (FM) formation, probably by regulating the folding of CLAVATA proteins (CLVs). Also involved in pollen tube elongation. Ref.1
Subunit structure	Interacts with FKBP42. Ref.7
Subcellular location	Endoplasmic reticulum lumen By similarity.
Tissue specificity	Ubiquitous. Ref.1
Induction	Seems inhibited by heat shock. Ref.1
Sequence similarities	Belongs to the heat shock protein 90 family.
Sequence caution	The sequence AAB63606.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Coding sequence diversity	Alternative splicing
Domain	Signal
Ligand	ATP-binding Calcium Nucleotide-binding
Molecular function	Chaperone
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein folding Inferred from electronic annotation. Source: InterPro protein secretion Inferred from direct assay PubMed 17059403. Source: TAIR regulation of meristem growth Inferred from mutant phenotype Ref.1. Source: TAIR regulation of meristem structural organization Inferred from mutant phenotype Ref.1. Source: TAIR response to cadmium ion Inferred from expression pattern PubMed 16502469. Source: TAIR response to cold Inferred from expression pattern PubMed 14617066. Source: TAIR response to salt stress Inferred from mutant phenotype PubMed 19148673. Source: TAIR response to water deprivation Inferred from mutant phenotype PubMed 19148673. Source: TAIR
Cellular_component	apoplast Inferred from direct assay PubMed 21798377. Source: TAIR chloroplast Inferred from direct assay PubMed 15028209PubMed 18431481. Source: TAIR endoplasmic reticulum Inferred from direct assay PubMed 16618929PubMed 17059403PubMed 22923678. Source: TAIR endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from direct assay Ref.8. Source: TAIR nucleus Inferred from direct assay PubMed 14617066. Source: TAIR plasma membrane Inferred from direct assay PubMed 17644812. Source: TAIR plasmodesma Inferred from direct assay PubMed 21533090. Source: TAIR vacuolar membrane Inferred from direct assay PubMed 17151019. Source: TAIR
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9STX5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 823	800	Endoplasmin homolog		PRO_0000226071
Regions
	Motif	820 – 823	4	Prevents secretion from ER By similarity
Sites
	Binding site	110	1	ATP By similarity
	Binding site	154	1	ATP By similarity
	Binding site	167	1	ATP By similarity
	Binding site	173	1	ATP By similarity
	Binding site	199	1	ATP; via amide nitrogen By similarity
	Binding site	455	1	ATP By similarity
Amino acid modifications
	Glycosylation	110	1	N-linked (GlcNAc...) Potential
	Glycosylation	452	1	N-linked (GlcNAc...) Potential
	Glycosylation	620	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 54C88E182A251990	FASTA	823	94,204
	10 20 30 40 50 60 MRKRTLVSVL FLFSLLFLLP DQGRKLHANA EESSDDVTDP PKVEEKIGGH GGLSTDSDVV 70 80 90 100 110 120 HRESESMSKK TLRSNAEKFE FQAEVSRLMD IIINSLYSNK DIFLRELISN ASDALDKIRF 130 140 150 160 170 180 LALTDKDVLG EGDTAKLEIQ IKLDKAKKIL SIRDRGIGMT KEDLIKNLGT IAKSGTSAFV 190 200 210 220 230 240 EKMQSSGDLN LIGQFGVGFY SAYLVADYIE VISKHNDDSQ YVWESKANGK FAVSEDTWNE 250 260 270 280 290 300 PLGRGTEIRL HLRDEAGEYL EESKLKELVK RYSEFINFPI SLWASKEVET EVPVEEDESA 310 320 330 340 350 360 DEETETTSTE EEKEEDAEEE DGEKKQKTKK VKETVYEWEL LNDVKAIWLR SPKEVTEEEY 370 380 390 400 410 420 TKFYHSLSKD FTDEKPMAWS HFNAEGDVEF KAVLYVPPKA PHDLYESYYN SNKANLKLYV 430 440 450 460 470 480 RRVFISDEFD ELLPKYLSFL KGLVDSDTLP LNVSREMLQQ HSSLKTIKKK LIRKALDMIR 490 500 510 520 530 540 KLAEEDPDEI HDDEKKDVEK SGENDEKKGQ YTKFWNEFGK SVKLGIIEDA ANRNRLAKLL 550 560 570 580 590 600 RFETTKSDGK LTSLDQYIKR MKKSQKDIFY ITGSSKEQLE KSPFLERLIK KGYEVIFFTD 610 620 630 640 650 660 PVDEYLMQYL MDYEDKKFQN VSKEGLKVGK DSKDKELKEA FKELTKWWKG NLASENVDDV 670 680 690 700 710 720 KISNRLADTP CVVVTSKFGW SANMERIMQS QTLSDANKQA YMRGKRVLEI NPRHPIIKEL 730 740 750 760 770 780 KDRIASDPED ESVKETAQLM YQTALIESGF ILTDPKDFAA RIYNSVKSGL NISPDAVADE 790 800 810 820 EIEAAEEPET SEATETKSDD LAGGLNIEAE PVEQQEENTK DEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"SHEPHERD is the Arabidopsis GRP94 responsible for the formation of functional CLAVATA proteins." Ishiguro S., Watanabe Y., Ito N., Nonaka H., Takeda N., Sakai T., Kanaya H., Okada K. EMBO J. 21:898-908(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-238. Strain: cv. Columbia.
[6]	"The Hsp90 family of proteins in Arabidopsis thaliana." Krishna P., Gloor G. Cell Stress Chaperones 6:238-246(2001) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[7]	"Characterization of Arabidopsis thaliana AtFKBP42 that is membrane-bound and interacts with Hsp90." Kamphausen T., Fanghaenel J., Neumann D., Schulz B., Rahfeld J.-U. Plant J. 32:263-276(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH FKBP42.
[8]	"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins." Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H. Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Strain: cv. Landsberg erecta.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB064528 mRNA. Translation: BAB86369.1. AC002343 Genomic DNA. Translation: AAB63606.1. Sequence problems. AL078637 Genomic DNA. Translation: CAB45054.1. AL161561 Genomic DNA. Translation: CAB79329.1. CP002687 Genomic DNA. Translation: AEE84861.1. AY039895 mRNA. Translation: AAK63999.1. AY072394 mRNA. Translation: AAL62386.1. BT004527 mRNA. Translation: AAO42773.1. AK221485 mRNA. Translation: BAD94659.1.
IPI	IPI00524027.
PIR	T09882.
RefSeq	NP_194150.1. NM_118552.3.
UniGene	At.24111.
3D structure databases
ProteinModelPortal	Q9STX5.
SMR	Q9STX5. Positions 75-776.
ModBase	Search...
Proteomic databases
PaxDb	Q9STX5.
PRIDE	Q9STX5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT4G24190.1; AT4G24190.1; AT4G24190.
GeneID	828520.
KEGG	ath:AT4G24190.
Organism-specific databases
TAIR	At4g24190.
Phylogenomic databases
eggNOG	COG0326.
HOGENOM	HOG000031988.
InParanoid	Q9STX5.
OMA	HITDTGI.
PhylomeDB	Q9STX5.
ProtClustDB	CLSN2716298.
Gene expression databases
ArrayExpress	Q9STX5.
Genevestigator	Q9STX5.
Family and domain databases
Gene3D	3.30.565.10. 1 hit.
InterPro	IPR015566. Endoplasmin. IPR003594. HATPase_ATP-bd. IPR019805. Heat_shock_protein_90_CS. IPR001404. Hsp90. IPR020575. Hsp90_N. IPR020568. Ribosomal_S5_D2-typ_fold. [Graphical view]
PANTHER	PTHR11528. PTHR11528. 1 hit. PTHR11528:SF21. PTHR11528:SF21. 1 hit.
Pfam	PF00183. HSP90. 1 hit. [Graphical view]
PIRSF	PIRSF002583. Hsp90. 1 hit.
PRINTS	PR00775. HEATSHOCK90.
SMART	SM00387. HATPase_c. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
PROSITE	PS00014. ER_TARGET. 1 hit. PS00298. HSP90. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ENPL_ARATH

Accession

Primary (citable) accession number: Q9STX5
Secondary accession number(s): O22972, Q56Y38, Q8VY71

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program