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Protein

Chloroplastic lipocalin

Gene

CHL

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Lipocalin that prevents thylakoidal membrane lipids peroxidation and confers protection against oxidative stress, especially mediated by singlet oxygen in response to high light and other stress (e.g. heat shocks) (PubMed:19674405, PubMed:23837879). Required for seed longevity by insuring polyunsaturated lipids integrity (PubMed:23837879).2 Publications

GO - Molecular functioni

GO - Biological processi

  • lipid metabolic process Source: UniProtKB-KW
  • negative regulation of fatty acid oxidation Source: UniProtKB
  • response to abscisic acid Source: UniProtKB
  • response to heat Source: UniProtKB
  • response to high light intensity Source: UniProtKB
  • response to oxidative stress Source: TAIR
  • response to paraquat Source: UniProtKB
  • response to water deprivation Source: UniProtKB
  • seed maturation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Seed storage protein, Storage protein

Keywords - Biological processi

Lipid metabolism

Enzyme and pathway databases

BioCyciARA:GQT-266-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chloroplastic lipocalin1 Publication
Short name:
AtCHL1 Publication
Gene namesi
Name:CHL1 Publication
Ordered Locus Names:At3g47860Imported
ORF Names:T23J7.190Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)Imported
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G47860.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast thylakoid lumen Source: UniProtKB-SubCell
  • chloroplast thylakoid membrane Source: TAIR
  • thylakoid lumen Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid, Thylakoid

Pathology & Biotechi

Disruption phenotypei

Increased sensitivity to photo-oxidative stress induced by drought, high light or paraquat, associated with a rapid accumulation of hydroxy fatty acids mediated by singlet oxygen (PubMed:19674405). When associated with disruption in TIL, highly sensitive to temperature, drought and light stresses than the single mutants, exhibiting intense lipid peroxidation. Seeds of this double mutant are very sensitive to natural and artificial aging, associated with the oxidation of polyunsaturated lipids (PubMed:23837879).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3939ChloroplastSequence analysisAdd
BLAST
Chaini40 – 353314Chloroplastic lipocalinSequence analysisPRO_0000434133Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi163 ↔ 299By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ9STS7.

PTM databases

iPTMnetiQ9STS7.

Expressioni

Tissue specificityi

Expressed in leaves at low levels (at protein levels) (PubMed:23837879). Present in seeds (PubMed:23837879).1 Publication

Inductioni

Accumulates in response to drought, high light, paraquat and abscisic acid (ABA) treatments (at protein level).1 Publication

Gene expression databases

GenevisibleiQ9STS7. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G47860.1.

Structurei

3D structure databases

ProteinModelPortaliQ9STS7.
SMRiQ9STS7. Positions 132-297.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi121 – 1299Met-richPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the calycin superfamily. Lipocalin family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG4824. Eukaryota.
COG3040. LUCA.
HOGENOMiHOG000029210.
OMAiAIQVDTF.
PhylomeDBiQ9STS7.

Family and domain databases

Gene3Di2.40.128.20. 1 hit.
InterProiIPR012674. Calycin.
IPR011038. Calycin-like.
IPR022272. Lipocalin_CS.
IPR000566. Lipocln_cytosolic_FA-bd_dom.
[Graphical view]
PfamiPF08212. Lipocalin_2. 1 hit.
[Graphical view]
SUPFAMiSSF50814. SSF50814. 1 hit.
PROSITEiPS00213. LIPOCALIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9STS7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILLSSSISL SRPVSSQSFS PPAATSTRRS HSSVTVKCCC SSRRLLKNPE
60 70 80 90 100
LKCSLENLFE IQALRKCFVS GFAAILLLSQ AGQGIALDLS SGYQNICQLG
110 120 130 140 150
SAAAVGENKL TLPSDGDSES MMMMMMRGMT AKNFDPVRYS GRWFEVASLK
160 170 180 190 200
RGFAGQGQED CHCTQGVYTF DMKESAIRVD TFCVHGSPDG YITGIRGKVQ
210 220 230 240 250
CVGAEDLEKS ETDLEKQEMI KEKCFLRFPT IPFIPKLPYD VIATDYDNYA
260 270 280 290 300
LVSGAKDKGF VQVYSRTPNP GPEFIAKYKN YLAQFGYDPE KIKDTPQDCE
310 320 330 340 350
VTDAELAAMM SMPGMEQTLT NQFPDLGLRK SVQFDPFTSV FETLKKLVPL

YFK
Length:353
Mass (Da):39,116
Last modified:May 1, 2000 - v1
Checksum:i14A83B0CF1BA8D0D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL049746 Genomic DNA. Translation: CAB41869.1.
CP002686 Genomic DNA. Translation: AEE78341.1.
AY035165 mRNA. Translation: AAK59669.1.
AY044334 mRNA. Translation: AAK73275.1.
BT003002 mRNA. Translation: AAO22810.1.
PIRiT07725.
RefSeqiNP_190370.1. NM_114656.3.
UniGeneiAt.3254.

Genome annotation databases

EnsemblPlantsiAT3G47860.1; AT3G47860.1; AT3G47860.
GeneIDi823942.
GrameneiAT3G47860.1; AT3G47860.1; AT3G47860.
KEGGiath:AT3G47860.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL049746 Genomic DNA. Translation: CAB41869.1.
CP002686 Genomic DNA. Translation: AEE78341.1.
AY035165 mRNA. Translation: AAK59669.1.
AY044334 mRNA. Translation: AAK73275.1.
BT003002 mRNA. Translation: AAO22810.1.
PIRiT07725.
RefSeqiNP_190370.1. NM_114656.3.
UniGeneiAt.3254.

3D structure databases

ProteinModelPortaliQ9STS7.
SMRiQ9STS7. Positions 132-297.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G47860.1.

PTM databases

iPTMnetiQ9STS7.

Proteomic databases

PaxDbiQ9STS7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G47860.1; AT3G47860.1; AT3G47860.
GeneIDi823942.
GrameneiAT3G47860.1; AT3G47860.1; AT3G47860.
KEGGiath:AT3G47860.

Organism-specific databases

TAIRiAT3G47860.

Phylogenomic databases

eggNOGiKOG4824. Eukaryota.
COG3040. LUCA.
HOGENOMiHOG000029210.
OMAiAIQVDTF.
PhylomeDBiQ9STS7.

Enzyme and pathway databases

BioCyciARA:GQT-266-MONOMER.

Miscellaneous databases

PROiQ9STS7.

Gene expression databases

GenevisibleiQ9STS7. AT.

Family and domain databases

Gene3Di2.40.128.20. 1 hit.
InterProiIPR012674. Calycin.
IPR011038. Calycin-like.
IPR022272. Lipocalin_CS.
IPR000566. Lipocln_cytosolic_FA-bd_dom.
[Graphical view]
PfamiPF08212. Lipocalin_2. 1 hit.
[Graphical view]
SUPFAMiSSF50814. SSF50814. 1 hit.
PROSITEiPS00213. LIPOCALIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "The chloroplastic lipocalin AtCHL prevents lipid peroxidation and protects Arabidopsis against oxidative stress."
    Levesque-Tremblay G., Havaux M., Ouellet F.
    Plant J. 60:691-702(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, INDUCTION BY DROUGHT; HIGH LIGHT; PARAQUAT AND ABSCISIC ACID TREATMENTS.
    Strain: cv. Columbia.
  5. "Arabidopsis lipocalins AtCHL and AtTIL have distinct but overlapping functions essential for lipid protection and seed longevity."
    Boca S., Koestler F., Ksas B., Chevalier A., Leymarie J., Fekete A., Mueller M.J., Havaux M.
    Plant Cell Environ. 37:368-381(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
    Strain: cv. Columbia.

Entry informationi

Entry nameiCHL_ARATH
AccessioniPrimary (citable) accession number: Q9STS7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 14, 2015
Last sequence update: May 1, 2000
Last modified: April 13, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.