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Protein

1-aminocyclopropane-1-carboxylate synthase 7

Gene

ACS7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

1-aminocyclopropane-1-carboxylate synthase (ACS) enzymes catalyze the conversion of S-adenosyl-L-methionine (SAM) into 1-aminocyclopropane-1-carboxylate (ACC), a direct precursor of ethylene.

Catalytic activityi

S-adenosyl-L-methionine = 1-aminocyclopropane-1-carboxylate + methylthioadenosine.1 Publication

Cofactori

Kineticsi

  1. KM=8.3 µM for AdoMet
  1. Vmax=13.5 µM/h/mg enzyme

pH dependencei

Optimum pH is 8.

Pathwayi: ethylene biosynthesis via S-adenosyl-L-methionine

This protein is involved in step 1 of the subpathway that synthesizes ethylene from S-adenosyl-L-methionine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 1-aminocyclopropane-1-carboxylate synthase 4 (ACS4), 1-aminocyclopropane-1-carboxylate synthase 8 (ACS8), 1-aminocyclopropane-1-carboxylate synthase 2 (ACS2), 1-aminocyclopropane-1-carboxylate synthase 7 (ACS7), 1-aminocyclopropane-1-carboxylate synthase 11 (ACS11), 1-aminocyclopropane-1-carboxylate synthase 9 (ACS9), 1-aminocyclopropane-1-carboxylate synthase 6 (ACS6), 1-aminocyclopropane-1-carboxylate synthase 5 (ACS5)
  2. 1-aminocyclopropane-1-carboxylate oxidase 2 (ACO2), 1-aminocyclopropane-1-carboxylate oxidase 3 (At1g12010), 1-aminocyclopropane-1-carboxylate oxidase 4 (ACO4), 1-aminocyclopropane-1-carboxylate oxidase 1 (ACO1), 1-aminocyclopropane-1-carboxylate oxidase 5 (At1g77330)
This subpathway is part of the pathway ethylene biosynthesis via S-adenosyl-L-methionine, which is itself part of Alkene biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ethylene from S-adenosyl-L-methionine, the pathway ethylene biosynthesis via S-adenosyl-L-methionine and in Alkene biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei61SubstrateBy similarity1
Binding sitei100SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • ethylene biosynthetic process Source: TAIR
  • fruit ripening Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Ethylene biosynthesis, Fruit ripening

Keywords - Ligandi

Pyridoxal phosphate, S-adenosyl-L-methionine

Enzyme and pathway databases

SABIO-RKQ9STR4.
UniPathwayiUPA00384; UER00562.

Names & Taxonomyi

Protein namesi
Recommended name:
1-aminocyclopropane-1-carboxylate synthase 7 (EC:4.4.1.14)
Short name:
ACC synthase 7
Alternative name(s):
S-adenosyl-L-methionine methylthioadenosine-lyase 7
Gene namesi
Name:ACS7
Ordered Locus Names:At4g26200
ORF Names:T25K17.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G26200.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001239011 – 4471-aminocyclopropane-1-carboxylate synthase 7Add BLAST447

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei285N6-(pyridoxal phosphate)lysineBy similarity1

Post-translational modificationi

Ubiquitinated by XBAT32. Ubiquitination probably leads to its subsequent degradation, thus controlling ethylene production.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ9STR4.

PTM databases

iPTMnetiQ9STR4.

Expressioni

Tissue specificityi

Expressed in roots.1 Publication

Inductioni

By cycloheximide (CHX).1 Publication

Gene expression databases

GenevisibleiQ9STR4. AT.

Interactioni

Subunit structurei

Homodimer and heterodimer. In vivo, the relevance of heterodimerization with other ACS enzymes is however unsure (By similarity). Interacts with XBAT32.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-2356842,EBI-2356842
ACS4Q433094EBI-2356842,EBI-2436015

Protein-protein interaction databases

BioGridi14013. 4 interactors.
IntActiQ9STR4. 2 interactors.
STRINGi3702.AT4G26200.1.

Structurei

3D structure databases

ProteinModelPortaliQ9STR4.
SMRiQ9STR4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0256. Eukaryota.
COG0436. LUCA.
HOGENOMiHOG000011234.
InParanoidiQ9STR4.
KOiK01762.
OMAiGEYIRTN.
OrthoDBiEOG09360ARA.
PhylomeDBiQ9STR4.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR004838. NHTrfase_class1_PyrdxlP-BS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00105. AA_TRANSFER_CLASS_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9STR4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLPLMMERS SNNNNVELSR VAVSDTHGED SPYFAGWKAY DENPYDESHN
60 70 80 90 100
PSGVIQMGLA ENQVSFDLLE TYLEKKNPEG SMWGSKGAPG FRENALFQDY
110 120 130 140 150
HGLKTFRQAM ASFMEQIRGG KARFDPDRIV LTAGATAANE LLTFILADPN
160 170 180 190 200
DALLVPTPYY PGFDRDLRWR TGVKIVPIHC DSSNHFQITP EALESAYQTA
210 220 230 240 250
RDANIRVRGV LITNPSNPLG ATVQKKVLED LLDFCVRKNI HLVSDEIYSG
260 270 280 290 300
SVFHASEFTS VAEIVENIDD VSVKERVHIV YSLSKDLGLP GFRVGTIYSY
310 320 330 340 350
NDNVVRTARR MSSFTLVSSQ TQHMLASMLS DEEFTEKYIR INRERLRRRY
360 370 380 390 400
DTIVEGLKKA GIECLKGNAG LFCWMNLGFL LEKKTKDGEL QLWDVILKEL
410 420 430 440
NLNISPGSSC HCSEVGWFRV CFANMSENTL EIALKRIHEF MDRRRRF
Length:447
Mass (Da):50,666
Last modified:May 1, 2000 - v1
Checksum:iE78CC1FC834FD4A9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL049171 Genomic DNA. Translation: CAB38949.1.
AL161564 Genomic DNA. Translation: CAB79475.1.
CP002687 Genomic DNA. Translation: AEE85169.1.
AF332390 mRNA. Translation: AAG48754.1.
PIRiT06004.
RefSeqiNP_194350.1. NM_118753.3.
UniGeneiAt.20362.

Genome annotation databases

EnsemblPlantsiAT4G26200.1; AT4G26200.1; AT4G26200.
GeneIDi828726.
GrameneiAT4G26200.1; AT4G26200.1; AT4G26200.
KEGGiath:AT4G26200.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL049171 Genomic DNA. Translation: CAB38949.1.
AL161564 Genomic DNA. Translation: CAB79475.1.
CP002687 Genomic DNA. Translation: AEE85169.1.
AF332390 mRNA. Translation: AAG48754.1.
PIRiT06004.
RefSeqiNP_194350.1. NM_118753.3.
UniGeneiAt.20362.

3D structure databases

ProteinModelPortaliQ9STR4.
SMRiQ9STR4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi14013. 4 interactors.
IntActiQ9STR4. 2 interactors.
STRINGi3702.AT4G26200.1.

PTM databases

iPTMnetiQ9STR4.

Proteomic databases

PaxDbiQ9STR4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G26200.1; AT4G26200.1; AT4G26200.
GeneIDi828726.
GrameneiAT4G26200.1; AT4G26200.1; AT4G26200.
KEGGiath:AT4G26200.

Organism-specific databases

TAIRiAT4G26200.

Phylogenomic databases

eggNOGiKOG0256. Eukaryota.
COG0436. LUCA.
HOGENOMiHOG000011234.
InParanoidiQ9STR4.
KOiK01762.
OMAiGEYIRTN.
OrthoDBiEOG09360ARA.
PhylomeDBiQ9STR4.

Enzyme and pathway databases

UniPathwayiUPA00384; UER00562.
SABIO-RKQ9STR4.

Miscellaneous databases

PROiQ9STR4.

Gene expression databases

GenevisibleiQ9STR4. AT.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR004838. NHTrfase_class1_PyrdxlP-BS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00105. AA_TRANSFER_CLASS_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry namei1A17_ARATH
AccessioniPrimary (citable) accession number: Q9STR4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The stability of ACS proteins, and the regulation of such stability, play a central role in ethylene biosynthesis.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.