Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9STP8

- ACBP2_ARATH

UniProt

Q9STP8 - ACBP2_ARATH

Protein

Acyl-CoA-binding domain-containing protein 2

Gene

ACBP2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Binds medium- and long-chain acyl-CoA esters with very high affinity. Can interact in vitro with palmitoyl-CoA, but not with oleoyl-CoA. Binds to lead ions (Pb). May function as an intracellular carrier of acyl-CoA esters. Required for proper phospholipid and, to a lower extent, galactolipid composition.4 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei162 – 1621Acyl-CoA
    Binding sitei181 – 1811Acyl-CoA

    GO - Molecular functioni

    1. fatty-acyl-CoA binding Source: UniProtKB
    2. lead ion binding Source: TAIR
    3. lipid binding Source: UniProtKB-KW
    4. protein binding Source: UniProtKB

    GO - Biological processi

    1. lipid transport Source: TAIR
    2. response to lead ion Source: UniProtKB

    Keywords - Biological processi

    Transport

    Keywords - Ligandi

    Lead, Lipid-binding, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acyl-CoA-binding domain-containing protein 2
    Short name:
    Acyl-CoA binding protein 2
    Gene namesi
    Name:ACBP2
    Ordered Locus Names:At4g27780
    ORF Names:T27E11.20
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G27780.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: TAIR
    2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    3. glyoxysome Source: UniProtKB
    4. integral component of membrane Source: UniProtKB-KW
    5. peroxisomal membrane Source: UniProtKB-SubCell
    6. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Endoplasmic reticulum, Membrane, Peroxisome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi136 – 1361Y → A: Loss of palmitoyl-CoA-binding activity. 1 Publication
    Mutagenesisi140 – 1401K → A: Loss of palmitoyl-CoA-binding activity. 1 Publication
    Mutagenesisi142 – 1421A → D: Slight increase of palmitoyl-CoA-binding activity. 1 Publication
    Mutagenesisi145 – 1451G → A: Normal palmitoyl-CoA-binding activity. 1 Publication
    Mutagenesisi152 – 1521P → A: Slight increase of palmitoyl-CoA-binding activity. 1 Publication
    Mutagenesisi162 – 1621K → A: Loss of palmitoyl-CoA-binding activity. 1 Publication
    Mutagenesisi163 – 1631W → A: Normal palmitoyl-CoA-binding activity. 1 Publication
    Mutagenesisi166 – 1661W → A: Slight increase of palmitoyl-CoA-binding activity. 1 Publication
    Mutagenesisi177 – 1771A → S: Slight increase of palmitoyl-CoA-binding activity. 1 Publication
    Mutagenesisi181 – 1811Y → A: Loss of palmitoyl-CoA-binding activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 354354Acyl-CoA-binding domain-containing protein 2PRO_0000379901Add
    BLAST

    Proteomic databases

    PaxDbiQ9STP8.
    PRIDEiQ9STP8.

    Expressioni

    Tissue specificityi

    Mostly expressed in roots and flowers, and, to a lower extent, in stems, pods and leaves (at protein level).2 Publications

    Inductioni

    In roots by lead.1 Publication

    Gene expression databases

    GenevestigatoriQ9STP8.

    Interactioni

    Subunit structurei

    Interacts (via ankyrin repeats) with HIPP26 and the ethylene-responsive element-binding proteins RAP2-3/EBP and RAP2-12. Interacts with CSE.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    HIPP26Q9SZN75EBI-368234,EBI-2008207
    RAP2-3P427363EBI-368234,EBI-368243

    Protein-protein interaction databases

    BioGridi14178. 3 interactions.
    DIPiDIP-32848N.
    IntActiQ9STP8. 3 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9STP8.
    SMRiQ9STP8. Positions 108-338.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei11 – 3121Helical; Signal-anchorSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini104 – 19491ACBPROSITE-ProRule annotationAdd
    BLAST
    Repeati265 – 29430ANK 1Add
    BLAST
    Repeati298 – 32730ANK 2Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni136 – 1405Acyl-CoA binding

    Sequence similaritiesi

    Belongs to the ACBP family.Curated
    Contains 1 ACB (acyl-CoA-binding) domain.PROSITE-ProRule annotation
    Contains 2 ANK repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    ANK repeat, Repeat, Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0666.
    HOGENOMiHOG000241133.
    InParanoidiQ9STP8.
    OMAiDSAKQAD.
    PhylomeDBiQ9STP8.

    Family and domain databases

    Gene3Di1.20.80.10. 1 hit.
    1.25.40.20. 1 hit.
    InterProiIPR000582. Acyl-CoA-binding_protein.
    IPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
    [Graphical view]
    PfamiPF00887. ACBP. 1 hit.
    PF12796. Ank_2. 1 hit.
    [Graphical view]
    PRINTSiPR00689. ACOABINDINGP.
    PR01415. ANKYRIN.
    SMARTiSM00248. ANK. 2 hits.
    [Graphical view]
    SUPFAMiSSF47027. SSF47027. 1 hit.
    SSF48403. SSF48403. 1 hit.
    PROSITEiPS51228. ACB_2. 1 hit.
    PS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9STP8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGDWAQLAQS VILGLIFSYL LAKLISIVVT FKEDNLSLTR HPEESQLEIK    50
    PEGVDSRRLD SSCGGFGGEA DSLVAEQGSS RSDSVAGDDS EEDDDWEGVE 100
    STELDEAFSA ATLFVTTAAA DRLSQKVPSD VQQQLYGLYK IATEGPCTAP 150
    QPSALKMTAR AKWQAWQKLG AMPPEEAMEK YIEIVTQLYP TWLDGGVKAG 200
    SRGGDDAASN SRGTMGPVFS SLVYDEESEN ELKIDAIHGF AREGEVENLL 250
    KSIESGIPVN ARDSEGRTPL HWAIDRGHLN IAKVLVDKNA DVNAKDNEGQ 300
    TPLHYAVVCD REAIAEFLVK QNANTAAKDE DGNSPLDLCE SDWPWIRDSA 350
    KQAD 354
    Length:354
    Mass (Da):38,480
    Last modified:May 1, 2000 - v1
    Checksum:i84DF085DB5FBD05B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF178947 mRNA. Translation: AAG46056.1.
    AF192307 Genomic DNA. Translation: AAG46057.1.
    AF320561 mRNA. Translation: AAK38078.1.
    AL078579 Genomic DNA. Translation: CAB43966.1.
    AL161571 Genomic DNA. Translation: CAB81427.1.
    CP002687 Genomic DNA. Translation: AEE85391.1.
    AY090256 mRNA. Translation: AAL90917.1.
    AY122892 mRNA. Translation: AAM67425.1.
    AY088510 mRNA. Translation: AAM66045.1.
    PIRiT09017.
    RefSeqiNP_194507.1. NM_118916.2.
    UniGeneiAt.25382.

    Genome annotation databases

    EnsemblPlantsiAT4G27780.1; AT4G27780.1; AT4G27780.
    GeneIDi828891.
    KEGGiath:AT4G27780.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF178947 mRNA. Translation: AAG46056.1 .
    AF192307 Genomic DNA. Translation: AAG46057.1 .
    AF320561 mRNA. Translation: AAK38078.1 .
    AL078579 Genomic DNA. Translation: CAB43966.1 .
    AL161571 Genomic DNA. Translation: CAB81427.1 .
    CP002687 Genomic DNA. Translation: AEE85391.1 .
    AY090256 mRNA. Translation: AAL90917.1 .
    AY122892 mRNA. Translation: AAM67425.1 .
    AY088510 mRNA. Translation: AAM66045.1 .
    PIRi T09017.
    RefSeqi NP_194507.1. NM_118916.2.
    UniGenei At.25382.

    3D structure databases

    ProteinModelPortali Q9STP8.
    SMRi Q9STP8. Positions 108-338.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 14178. 3 interactions.
    DIPi DIP-32848N.
    IntActi Q9STP8. 3 interactions.

    Proteomic databases

    PaxDbi Q9STP8.
    PRIDEi Q9STP8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G27780.1 ; AT4G27780.1 ; AT4G27780 .
    GeneIDi 828891.
    KEGGi ath:AT4G27780.

    Organism-specific databases

    TAIRi AT4G27780.

    Phylogenomic databases

    eggNOGi COG0666.
    HOGENOMi HOG000241133.
    InParanoidi Q9STP8.
    OMAi DSAKQAD.
    PhylomeDBi Q9STP8.

    Gene expression databases

    Genevestigatori Q9STP8.

    Family and domain databases

    Gene3Di 1.20.80.10. 1 hit.
    1.25.40.20. 1 hit.
    InterProi IPR000582. Acyl-CoA-binding_protein.
    IPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
    [Graphical view ]
    Pfami PF00887. ACBP. 1 hit.
    PF12796. Ank_2. 1 hit.
    [Graphical view ]
    PRINTSi PR00689. ACOABINDINGP.
    PR01415. ANKYRIN.
    SMARTi SM00248. ANK. 2 hits.
    [Graphical view ]
    SUPFAMi SSF47027. SSF47027. 1 hit.
    SSF48403. SSF48403. 1 hit.
    PROSITEi PS51228. ACB_2. 1 hit.
    PS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Single amino acid substitutions at the acyl-CoA-binding domain interrupt 14[C]palmitoyl-CoA binding of ACBP2, an Arabidopsis acyl-CoA-binding protein with ankyrin repeats."
      Chye M.-L., Li H.-Y., Yung M.-H.
      Plant Mol. Biol. 44:711-721(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, MUTAGENESIS OF TYR-136; LYS-140; LYS-162 AND TYR-181, TISSUE SPECIFICITY.
      Strain: cv. C24 and cv. Columbia.
      Tissue: Flower.
    2. "The effects of down-regulating expression of Arabidopsis thaliana membrane-associated acyl-CoA binding protein 2 on acyl-lipid composition."
      Kojima M., Casteel J., Miernyk J.A., Thelen J.J.
      Plant Sci. 172:36-44(2007)
      [AGRICOLA] [Europe PMC]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, IDENTIFICATION BY MASS SPECTROMETRY.
    3. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    7. "Novel glyoxysomal protein kinase, GPK1, identified by proteomic analysis of glyoxysomes in etiolated cotyledons of Arabidopsis thaliana."
      Fukao Y., Hayashi M., Hara-Nishimura I., Nishimura M.
      Plant Cell Physiol. 44:1002-1012(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
    8. "Membrane localization of Arabidopsis acyl-CoA binding protein ACBP2."
      Li H.-Y., Chye M.-L.
      Plant Mol. Biol. 51:483-492(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, SIGNAL-ANCHOR.
    9. "Arabidopsis Acyl-CoA-binding protein ACBP2 interacts with an ethylene-responsive element-binding protein, AtEBP, via its ankyrin repeats."
      Li H.-Y., Chye M.-L.
      Plant Mol. Biol. 54:233-243(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH EBP.
    10. "ACBP4 and ACBP5, novel Arabidopsis acyl-CoA-binding proteins with kelch motifs that bind oleoyl-CoA."
      Leung K.-C., Li H.-Y., Mishra G., Chye M.-L.
      Plant Mol. Biol. 55:297-309(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF ALA-142; GLY-145; PRO-152; TRP-163; TRP-166 AND ALA-177.
    11. "Overexpression of membrane-associated acyl-CoA-binding protein ACBP1 enhances lead tolerance in Arabidopsis."
      Xiao S., Gao W., Chen Q.-F., Ramalingam S., Chye M.-L.
      Plant J. 54:141-151(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION BY LEAD.
    12. "Arabidopsis thaliana acyl-CoA-binding protein ACBP2 interacts with heavy-metal-binding farnesylated protein AtFP6."
      Gao W., Xiao S., Li H.Y., Tsao S.W., Chye M.L.
      New Phytol. 181:89-102(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HIPP26.
    13. "An Arabidopsis family of six acyl-CoA-binding proteins has three cytosolic members."
      Xiao S., Chye M.-L.
      Plant Physiol. Biochem. 47:479-484(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    14. "Acyl-CoA-binding protein 2 binds lysophospholipase 2 and lysoPC to promote tolerance to cadmium-induced oxidative stress in transgenic Arabidopsis."
      Gao W., Li H.Y., Xiao S., Chye M.L.
      Plant J. 62:989-1003(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CSE, SUBCELLULAR LOCATION.
    15. "Oxygen sensing in plants is mediated by an N-end rule pathway for protein destabilization."
      Licausi F., Kosmacz M., Weits D.A., Giuntoli B., Giorgi F.M., Voesenek L.A., Perata P., van Dongen J.T.
      Nature 479:419-422(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RAP2-12.

    Entry informationi

    Entry nameiACBP2_ARATH
    AccessioniPrimary (citable) accession number: Q9STP8
    Secondary accession number(s): Q9FR48
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3