Q9STL4 (CEP2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
KDEL-tailed cysteine endopeptidase CEP2
EC=3.4.22.-

Gene names

Name:	CEP2
Ordered Locus Names:	At3g48340
ORF Names:	T29H11.140

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the final stage of developmental programmed cell death and in intercalation of new cells. Cleaves extensins, thus probably supporing the final cell collapse By similarity. Ref.3
Subcellular location	Endoplasmic reticulum Probable.
Tissue specificity	Expressed in roots, stems, rosette and cauline leaves, flowers, buds and green siliques. Found in the tip of young primary leaves, in very young root tips and at later stages in all tissues of lateral root, including the vascular bundle. Not expressed in lateral root primordia, while directly emerging through the epidermis. Ref.3
Sequence similarities	Belongs to the peptidase C1 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 361	341	KDEL-tailed cysteine endopeptidase CEP2		PRO_0000403790
Regions
	Motif	358 – 361	4	Prevents secretion from ER Potential
Sites
	Active site	152	1	By similarity
	Active site	288	1	By similarity
	Active site	308	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9STL4 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: FEF1381FFE780C6E	FASTA	361	40,371
	10 20 30 40 50 60 MKKLLLIFLF SLVILQTACG FDYDDKEIES EEGLSTLYDR WRSHHSVPRS LNEREKRFNV 70 80 90 100 110 120 FRHNVMHVHN TNKKNRSYKL KLNKFADLTI NEFKNAYTGS NIKHHRMLQG PKRGSKQFMY 130 140 150 160 170 180 DHENLSKLPS SVDWRKKGAV TEIKNQGKCG SCWAFSTVAA VEGINKIKTN KLVSLSEQEL 190 200 210 220 230 240 VDCDTKQNEG CNGGLMEIAF EFIKKNGGIT TEDSYPYEGI DGKCDASKDN GVLVTIDGHE 250 260 270 280 290 300 DVPENDENAL LKAVANQPVS VAIDAGSSDF QFYSEGVFTG SCGTELNHGV AAVGYGSERG 310 320 330 340 350 360 KKYWIVRNSW GAEWGEGGYI KIEREIDEPE GRCGIAMEAS YPIKLSSSNP TPKDGDVKDE L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"KDEL-tailed cysteine endopeptidases involved in programmed cell death, intercalation of new cells, and dismantling of extensin scaffolds." Helm M., Schmid M., Hierl G., Terneus K., Tan L., Lottspeich F., Kieliszewski M.J., Gietl C. Am. J. Bot. 95:1049-1062(2008) Cited for: FUNCTION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL049659 Genomic DNA. Translation: CAB41164.1. CP002686 Genomic DNA. Translation: AEE78403.1.
IPI	IPI00524755.
PIR	T06708.
RefSeq	NP_680113.3. NM_148860.4.
UniGene	At.46818.
3D structure databases
ProteinModelPortal	Q9STL4.
SMR	Q9STL4. Positions 28-351.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9STL4. 1 interaction.
STRING	3702.AT3G48340.1-P.
Protein family/group databases
MEROPS	I29.003.
Proteomic databases
PaxDb	Q9STL4.
PRIDE	Q9STL4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G48340.1; AT3G48340.1; AT3G48340.
GeneID	823992.
KEGG	ath:AT3G48340.
Organism-specific databases
TAIR	At3g48340.
Phylogenomic databases
eggNOG	COG4870.
HOGENOM	HOG000230773.
InParanoid	Q9STL4.
KO	K16292.
OMA	GLMEIAF.
PhylomeDB	Q9STL4.
Gene expression databases
Genevestigator	Q9STL4.
Family and domain databases
InterPro	IPR025661. Pept_asp_AS. IPR000169. Pept_cys_AS. IPR025660. Pept_his_AS. IPR013128. Peptidase_C1A. IPR000668. Peptidase_C1A_C. IPR013201. Prot_inhib_I29. [Graphical view]
PANTHER	PTHR12411. PTHR12411. 1 hit.
Pfam	PF08246. Inhibitor_I29. 1 hit. PF00112. Peptidase_C1. 1 hit. [Graphical view]
PRINTS	PR00705. PAPAIN.
SMART	SM00848. Inhibitor_I29. 1 hit. SM00645. Pept_C1. 1 hit. [Graphical view]
PROSITE	PS00014. ER_TARGET. 1 hit. PS00640. THIOL_PROTEASE_ASN. 1 hit. PS00139. THIOL_PROTEASE_CYS. 1 hit. PS00639. THIOL_PROTEASE_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CEP2_ARATH

Accession

Primary (citable) accession number: Q9STL4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2011
Last sequence update:	May 1, 2000
Last modified:	May 29, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program