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Protein

Amidophosphoribosyltransferase 2, chloroplastic

Gene

ASE2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the first committed step of 'de novo purine biosynthesis from glutamine. Required for chloroplast biogenesis and cell division. Confers sensitivity to the phenyltriazole acetic acid compound [5-(4-chlorophenyl)-1-isopropyl-1H-[1,2,4]triazol-3-yl]-acetic acid (DAS734), a bleaching herbicide.3 Publications

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.1 Publication

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster per subunit.By similarity
  • Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Enzyme regulationi

Inhibited by the phenyltriazole acetic acid compound [5-(4-chlorophenyl)-1-isopropyl-1H-[1,2,4]triazol-3-yl]-acetic acid (DAS734), a bleaching herbicide.1 Publication

Kineticsi

  1. KM=1.34 mM for glutamine1 Publication

    Pathwayi: IMP biosynthesis via de novo pathway

    This protein is involved in step 1 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. Amidophosphoribosyltransferase 2, chloroplastic (ASE2), Amidophosphoribosyltransferase (AXX17_At4g39730), Amidophosphoribosyltransferase 1, chloroplastic (ASE1), Amidophosphoribosyltransferase 3, chloroplastic (ASE3), Amidophosphoribosyltransferase (AXX17_At4g44210), Amidophosphoribosyltransferase (AXX17_At2g11630)
    2. Phosphoribosylamine--glycine ligase, chloroplastic (PUR2)
    This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei87NucleophilePROSITE-ProRule annotation1
    Metal bindingi323Iron-sulfur (4Fe-4S)By similarity1
    Metal bindingi469Iron-sulfur (4Fe-4S)By similarity1
    Metal bindingi520Iron-sulfur (4Fe-4S)By similarity1
    Metal bindingi523Iron-sulfur (4Fe-4S)By similarity1

    GO - Molecular functioni

    • amidophosphoribosyltransferase activity Source: TAIR
    • iron-sulfur cluster binding Source: UniProtKB-KW
    • metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    • 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
    • chloroplast organization Source: TAIR
    • glutamine metabolic process Source: UniProtKB-KW
    • nucleoside metabolic process Source: InterPro
    • purine nucleobase biosynthetic process Source: InterPro
    • purine nucleotide biosynthetic process Source: TAIR

    Keywordsi

    Molecular functionGlycosyltransferase, Transferase
    Biological processPurine biosynthesis
    LigandIron, Iron-sulfur, Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT4G34740-MONOMER
    BRENDAi2.4.2.14 399
    ReactomeiR-ATH-73817 Purine ribonucleoside monophosphate biosynthesis
    UniPathwayiUPA00074; UER00124

    Protein family/group databases

    MEROPSiC44.A03

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Amidophosphoribosyltransferase 2, chloroplastic (EC:2.4.2.14)
    Short name:
    AtATase2
    Short name:
    AtPURF2
    Short name:
    PRPP2
    Alternative name(s):
    Glutamine phosphoribosylpyrophosphate amidotransferase 2
    Short name:
    AtGPRAT2
    Protein CHLOROPLAST IMPORT APPARATUS 1
    Protein DIFFERENTIAL DEVELOPMENT OF VASCULAR ASSOCIATED CELLS
    Gene namesi
    Name:ASE2
    Synonyms:CIA1, DOV1, GPRAT2, PURF2
    Ordered Locus Names:At4g34740
    ORF Names:T4L20.320
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 4

    Organism-specific databases

    AraportiAT4G34740
    TAIRilocus:2139549 AT4G34740

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Chloroplast, Plastid

    Pathology & Biotechi

    Disruption phenotypei

    Strong growth retardation and severe chlorosis in leaves; white leaves, but green cotyledons. Leaves missing the palisade mesophyll layer, due to reduced cell number and size. Abnormal thylakoid membrane in chloroplasts, probably due to photo-oxidative damage. Defective in protein import into chloroplasts.2 Publications

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi187H → T in cia1-2; small plants with white leaves showing an irregular mosaic of green sectors. 1 Publication1
    Mutagenesisi264R → K: Strong resistance to the bleaching herbicides DAS073 and DAS734. 1 Publication1
    Mutagenesisi265P → S: Low resistance to the bleaching herbicides DAS073 and DAS734; when associated with F-494. 1 Publication1
    Mutagenesisi371G → S: Low resistance to the bleaching herbicides DAS073 and DAS734. 1 Publication1
    Mutagenesisi476P → S: Resistance to the bleaching herbicides DAS073 and DAS734. 1 Publication1
    Mutagenesisi494Y → F: Low resistance to the bleaching herbicides DAS073 and DAS734; when associated with S-265. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transit peptidei1 – 53ChloroplastSequence analysisAdd BLAST53
    ChainiPRO_000042028254 – 561Amidophosphoribosyltransferase 2, chloroplasticAdd BLAST508

    Proteomic databases

    PaxDbiQ9STG9
    PRIDEiQ9STG9

    PTM databases

    SwissPalmiQ9STG9

    Expressioni

    Tissue specificityi

    Mostly expressed in leaves, and, to a lower extent, in cotyledons.3 Publications

    Gene expression databases

    ExpressionAtlasiQ9STG9 baseline and differential
    GenevisibleiQ9STG9 AT

    Interactioni

    Protein-protein interaction databases

    BioGridi14908, 5 interactors
    IntActiQ9STG9, 4 interactors
    STRINGi3702.AT4G34740.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ9STG9
    SMRiQ9STG9
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini87 – 307Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST221

    Sequence similaritiesi

    In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated

    Keywords - Domaini

    Glutamine amidotransferase, Transit peptide

    Phylogenomic databases

    eggNOGiKOG0572 Eukaryota
    COG0034 LUCA
    HOGENOMiHOG000033688
    InParanoidiQ9STG9
    KOiK00764
    OMAiKHECGIA
    OrthoDBiEOG093609B8
    PhylomeDBiQ9STG9

    Family and domain databases

    CDDicd00715 GPATase_N, 1 hit
    cd06223 PRTases_typeI, 1 hit
    Gene3Di3.60.20.10, 3 hits
    HAMAPiMF_01931 PurF, 1 hit
    InterProiView protein in InterPro
    IPR017932 GATase_2_dom
    IPR029055 Ntn_hydrolases_N
    IPR000836 PRibTrfase_dom
    IPR029057 PRTase-like
    IPR005854 PurF
    IPR035584 PurF_N
    PfamiView protein in Pfam
    PF13537 GATase_7, 1 hit
    PF00156 Pribosyltran, 1 hit
    PIRSFiPIRSF000485 Amd_phspho_trans, 1 hit
    SUPFAMiSSF53271 SSF53271, 1 hit
    SSF56235 SSF56235, 1 hit
    TIGRFAMsiTIGR01134 purF, 1 hit
    PROSITEiView protein in PROSITE
    PS51278 GATASE_TYPE_2, 1 hit
    PS00103 PUR_PYR_PR_TRANSFER, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9STG9-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAATSSISSS LSLNAKPNKL SNNNNNNKPH RFLRNPFLNP SSSSFSPLPA
    60 70 80 90 100
    SISSSSSSPS FPLRVSNPLT LLAADNDDYD EKPREECGVV GIYGDSEASR
    110 120 130 140 150
    LCYLALHALQ HRGQEGAGIV TVSKDKVLQT ITGVGLVSEV FSESKLDQLP
    160 170 180 190 200
    GDIAIGHVRY STAGSSMLKN VQPFVAGYRF GSVGVAHNGN LVNYTKLRAD
    210 220 230 240 250
    LEENGSIFNT SSDTEVVLHL IAISKARPFF MRIVDACEKL QGAYSMVFVT
    260 270 280 290 300
    EDKLVAVRDP HGFRPLVMGR RSNGAVVFAS ETCALDLIEA TYEREVYPGE
    310 320 330 340 350
    VLVVDKDGVK CQCLMPHPEP KQCIFEHIYF SLPNSIVFGR SVYESRHVFG
    360 370 380 390 400
    EILATESPVD CDVVIAVPDS GVVAALGYAA KAGVAFQQGL IRSHYVGRTF
    410 420 430 440 450
    IEPSQKIRDF GVKLKLSPVR GVLEGKRVVV VDDSIVRGTT SSKIVRLLRE
    460 470 480 490 500
    AGAKEVHMRI ASPPIIASCY YGVDTPSSNE LISNRMSVDE IRDYIGCDSL
    510 520 530 540 550
    AFLSFETLKK HLGEDSRSFC YACFTGDYPV KPTEDKVKRG GDFIDDGLVG
    560
    GIHNIEGGWV R
    Length:561
    Mass (Da):61,030
    Last modified:May 1, 2000 - v1
    Checksum:iAE52BD02CE304796
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti5S → C in AAW28080 (Ref. 1) Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY842241 Genomic DNA Translation: AAW28080.1
    AL023094 Genomic DNA Translation: CAA18853.1
    AL161586 Genomic DNA Translation: CAB80191.1
    CP002687 Genomic DNA Translation: AEE86417.1
    AY065123 mRNA Translation: AAL38299.1
    AY081611 mRNA Translation: AAM10173.1
    D28869 mRNA Translation: BAA06024.1
    PIRiT05294
    RefSeqiNP_195200.1, NM_119640.3
    UniGeneiAt.21088

    Genome annotation databases

    EnsemblPlantsiAT4G34740.1; AT4G34740.1; AT4G34740
    GeneIDi829626
    GrameneiAT4G34740.1; AT4G34740.1; AT4G34740
    KEGGiath:AT4G34740

    Similar proteinsi

    Entry informationi

    Entry nameiASE2_ARATH
    AccessioniPrimary (citable) accession number: Q9STG9
    Secondary accession number(s): Q39000, Q5MAT8
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2012
    Last sequence update: May 1, 2000
    Last modified: May 23, 2018
    This is version 135 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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