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Protein

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Gene

DUT

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP, preventing uracil incorporation into DNA.1 Publication

Miscellaneous

Silencing of DUT leads to high seedling mortality and affects plant growth and flower organ morphology in surviving plants.

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.

Cofactori

Mg2+Note: Binds 1 Mg2+ per trimer.

Pathwayi: dUMP biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes dUMP from dCTP (dUTP route).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Deoxyuridine 5'-triphosphate nucleotidohydrolase (DUT)
This subpathway is part of the pathway dUMP biosynthesis, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dUMP from dCTP (dUTP route), the pathway dUMP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi138Magnesium; shared with trimeric partners1 Publication1

GO - Molecular functioni

  • dUTP diphosphatase activity Source: GO_Central
  • identical protein binding Source: UniProtKB
  • magnesium ion binding Source: UniProtKB

GO - Biological processi

  • DNA repair Source: TAIR
  • dUMP biosynthetic process Source: GO_Central
  • dUTP catabolic process Source: GO_Central

Keywordsi

Molecular functionHydrolase
Biological processNucleotide metabolism
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT3G46940-MONOMER
MetaCyc:AT3G46940-MONOMER
BRENDAi3.6.1.23 399
ReactomeiR-ATH-499943 Interconversion of nucleotide di- and triphosphates
UniPathwayiUPA00610; UER00666

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase (EC:3.6.1.23)
Short name:
dUTPase
Alternative name(s):
dUTP pyrophosphatase
dUTP-pyrophosphatase-like 1
Short name:
AtDUT1
Gene namesi
Name:DUT
Synonyms:DUT1
Ordered Locus Names:At3g46940
ORF Names:T6H20.30
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G46940
TAIRilocus:2102817 AT3G46940

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004013661 – 166Deoxyuridine 5'-triphosphate nucleotidohydrolaseAdd BLAST166

Proteomic databases

PaxDbiQ9STG6
PRIDEiQ9STG6

PTM databases

iPTMnetiQ9STG6

Expressioni

Gene expression databases

ExpressionAtlasiQ9STG6 baseline and differential
GenevisibleiQ9STG6 AT

Interactioni

Subunit structurei

Homotrimer.1 Publication

GO - Molecular functioni

  • identical protein binding Source: UniProtKB

Protein-protein interaction databases

BioGridi9167, 1 interactor
STRINGi3702.AT3G46940.1

Structurei

Secondary structure

1166
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi30 – 35Combined sources6
Beta strandi42 – 45Combined sources4
Beta strandi51 – 54Combined sources4
Beta strandi59 – 61Combined sources3
Beta strandi66 – 70Combined sources5
Beta strandi73 – 76Combined sources4
Beta strandi81 – 86Combined sources6
Helixi89 – 95Combined sources7
Beta strandi97 – 100Combined sources4
Beta strandi103 – 105Combined sources3
Beta strandi113 – 118Combined sources6
Beta strandi120 – 122Combined sources3
Beta strandi124 – 126Combined sources3
Beta strandi131 – 141Combined sources11
Beta strandi145 – 147Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PC5X-ray2.20A/B/C1-166[»]
4OOPX-ray1.50A/B/C1-166[»]
4OOQX-ray2.00A/B/C1-166[»]
ProteinModelPortaliQ9STG6
SMRiQ9STG6
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9STG6

Family & Domainsi

Sequence similaritiesi

Belongs to the dUTPase family.Curated

Phylogenomic databases

eggNOGiKOG3370 Eukaryota
COG0756 LUCA
HOGENOMiHOG000028966
KOiK01520
OMAiGVILINH
OrthoDBiEOG09360O3G
PhylomeDBiQ9STG6

Family and domain databases

CDDicd07557 trimeric_dUTPase, 1 hit
Gene3Di2.70.40.10, 1 hit
InterProiView protein in InterPro
IPR029054 dUTPase-like
IPR036157 dUTPase-like_sf
IPR008181 dUTPase_1
IPR033704 dUTPase_trimeric
PfamiView protein in Pfam
PF00692 dUTPase, 1 hit
SUPFAMiSSF51283 SSF51283, 1 hit
TIGRFAMsiTIGR00576 dut, 1 hit

Sequencei

Sequence statusi: Complete.

Q9STG6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MACVNEPSPK LQKLDRNGIH GDSSPSPFFK VKKLSEKAVI PTRGSPLSAG
60 70 80 90 100
YDLSSAVDSK VPARGKALIP TDLSIAVPEG TYARIAPRSG LAWKHSIDVG
110 120 130 140 150
AGVIDADYRG PVGVILFNHS DADFEVKFGD RIAQLIIEKI VTPDVVEVDD
160
LDETVRGDGG FGSTGV
Length:166
Mass (Da):17,557
Last modified:May 1, 2000 - v1
Checksum:i5487738DF4A55BFF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL096859 Genomic DNA Translation: CAB51171.1
CP002686 Genomic DNA Translation: AEE78222.1
AF370334 mRNA Translation: AAK44149.1
AY062989 mRNA Translation: AAL34163.1
PIRiT12954
RefSeqiNP_190278.1, NM_114561.4
UniGeneiAt.48742
At.71138

Genome annotation databases

EnsemblPlantsiAT3G46940.1; AT3G46940.1; AT3G46940
AT3G46940.2; AT3G46940.2; AT3G46940
GeneIDi823847
GrameneiAT3G46940.1; AT3G46940.1; AT3G46940
AT3G46940.2; AT3G46940.2; AT3G46940
KEGGiath:AT3G46940

Similar proteinsi

Entry informationi

Entry nameiDUT_ARATH
AccessioniPrimary (citable) accession number: Q9STG6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: May 1, 2000
Last modified: April 25, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

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