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Protein

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Gene

DUT

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP, preventing uracil incorporation into DNA.1 Publication

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.

Cofactori

Mg2+Note: Binds 1 Mg2+ per trimer.

Pathwayi: dUMP biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes dUMP from dCTP (dUTP route).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Deoxyuridine 5'-triphosphate nucleotidohydrolase (DUT)
This subpathway is part of the pathway dUMP biosynthesis, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dUMP from dCTP (dUTP route), the pathway dUMP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi138Magnesium; shared with trimeric partners1 Publication1

GO - Molecular functioni

  • dUTP diphosphatase activity Source: GO_Central
  • identical protein binding Source: UniProtKB
  • magnesium ion binding Source: UniProtKB

GO - Biological processi

  • DNA repair Source: TAIR
  • dUMP biosynthetic process Source: GO_Central
  • dUTP catabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT3G46940-MONOMER.
MetaCyc:AT3G46940-MONOMER.
BRENDAi3.6.1.23. 399.
ReactomeiR-ATH-500753. Pyrimidine biosynthesis.
UniPathwayiUPA00610; UER00666.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase (EC:3.6.1.23)
Short name:
dUTPase
Alternative name(s):
dUTP pyrophosphatase
dUTP-pyrophosphatase-like 1
Short name:
AtDUT1
Gene namesi
Name:DUT
Synonyms:DUT1
Ordered Locus Names:At3g46940
ORF Names:T6H20.30
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G46940.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004013661 – 166Deoxyuridine 5'-triphosphate nucleotidohydrolaseAdd BLAST166

Proteomic databases

PaxDbiQ9STG6.
PRIDEiQ9STG6.

PTM databases

iPTMnetiQ9STG6.

Expressioni

Gene expression databases

GenevisibleiQ9STG6. AT.

Interactioni

Subunit structurei

Homotrimer.1 Publication

GO - Molecular functioni

  • identical protein binding Source: UniProtKB

Protein-protein interaction databases

BioGridi9167. 1 interactor.
STRINGi3702.AT3G46940.1.

Structurei

Secondary structure

1166
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi30 – 35Combined sources6
Beta strandi42 – 45Combined sources4
Beta strandi51 – 54Combined sources4
Beta strandi59 – 61Combined sources3
Beta strandi66 – 70Combined sources5
Beta strandi73 – 76Combined sources4
Beta strandi81 – 86Combined sources6
Helixi89 – 95Combined sources7
Beta strandi97 – 100Combined sources4
Beta strandi103 – 105Combined sources3
Beta strandi113 – 118Combined sources6
Beta strandi120 – 122Combined sources3
Beta strandi124 – 126Combined sources3
Beta strandi131 – 141Combined sources11
Beta strandi145 – 147Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PC5X-ray2.20A/B/C1-166[»]
4OOPX-ray1.50A/B/C1-166[»]
4OOQX-ray2.00A/B/C1-166[»]
ProteinModelPortaliQ9STG6.
SMRiQ9STG6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9STG6.

Family & Domainsi

Sequence similaritiesi

Belongs to the dUTPase family.Curated

Phylogenomic databases

eggNOGiKOG3370. Eukaryota.
COG0756. LUCA.
HOGENOMiHOG000028966.
KOiK01520.
OMAiKICYPEL.
OrthoDBiEOG09360O3G.
PhylomeDBiQ9STG6.

Family and domain databases

CDDicd07557. trimeric_dUTPase. 1 hit.
Gene3Di2.70.40.10. 1 hit.
InterProiIPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR008181. dUTPase_1.
IPR033704. dUTPase_trimeric.
[Graphical view]
PfamiPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMiSSF51283. SSF51283. 1 hit.
TIGRFAMsiTIGR00576. dut. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9STG6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MACVNEPSPK LQKLDRNGIH GDSSPSPFFK VKKLSEKAVI PTRGSPLSAG
60 70 80 90 100
YDLSSAVDSK VPARGKALIP TDLSIAVPEG TYARIAPRSG LAWKHSIDVG
110 120 130 140 150
AGVIDADYRG PVGVILFNHS DADFEVKFGD RIAQLIIEKI VTPDVVEVDD
160
LDETVRGDGG FGSTGV
Length:166
Mass (Da):17,557
Last modified:May 1, 2000 - v1
Checksum:i5487738DF4A55BFF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL096859 Genomic DNA. Translation: CAB51171.1.
CP002686 Genomic DNA. Translation: AEE78222.1.
AF370334 mRNA. Translation: AAK44149.1.
AY062989 mRNA. Translation: AAL34163.1.
PIRiT12954.
RefSeqiNP_190278.1. NM_114561.4.
UniGeneiAt.48742.
At.71138.

Genome annotation databases

EnsemblPlantsiAT3G46940.1; AT3G46940.1; AT3G46940.
GeneIDi823847.
GrameneiAT3G46940.1; AT3G46940.1; AT3G46940.
KEGGiath:AT3G46940.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL096859 Genomic DNA. Translation: CAB51171.1.
CP002686 Genomic DNA. Translation: AEE78222.1.
AF370334 mRNA. Translation: AAK44149.1.
AY062989 mRNA. Translation: AAL34163.1.
PIRiT12954.
RefSeqiNP_190278.1. NM_114561.4.
UniGeneiAt.48742.
At.71138.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PC5X-ray2.20A/B/C1-166[»]
4OOPX-ray1.50A/B/C1-166[»]
4OOQX-ray2.00A/B/C1-166[»]
ProteinModelPortaliQ9STG6.
SMRiQ9STG6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi9167. 1 interactor.
STRINGi3702.AT3G46940.1.

PTM databases

iPTMnetiQ9STG6.

Proteomic databases

PaxDbiQ9STG6.
PRIDEiQ9STG6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G46940.1; AT3G46940.1; AT3G46940.
GeneIDi823847.
GrameneiAT3G46940.1; AT3G46940.1; AT3G46940.
KEGGiath:AT3G46940.

Organism-specific databases

TAIRiAT3G46940.

Phylogenomic databases

eggNOGiKOG3370. Eukaryota.
COG0756. LUCA.
HOGENOMiHOG000028966.
KOiK01520.
OMAiKICYPEL.
OrthoDBiEOG09360O3G.
PhylomeDBiQ9STG6.

Enzyme and pathway databases

UniPathwayiUPA00610; UER00666.
BioCyciARA:AT3G46940-MONOMER.
MetaCyc:AT3G46940-MONOMER.
BRENDAi3.6.1.23. 399.
ReactomeiR-ATH-500753. Pyrimidine biosynthesis.

Miscellaneous databases

EvolutionaryTraceiQ9STG6.
PROiQ9STG6.

Gene expression databases

GenevisibleiQ9STG6. AT.

Family and domain databases

CDDicd07557. trimeric_dUTPase. 1 hit.
Gene3Di2.70.40.10. 1 hit.
InterProiIPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR008181. dUTPase_1.
IPR033704. dUTPase_trimeric.
[Graphical view]
PfamiPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMiSSF51283. SSF51283. 1 hit.
TIGRFAMsiTIGR00576. dut. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDUT_ARATH
AccessioniPrimary (citable) accession number: Q9STG6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Silencing of DUT leads to high seedling mortality and affects plant growth and flower organ morphology in surviving plants.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.