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Q9STG6

- DUT_ARATH

UniProt

Q9STG6 - DUT_ARATH

Protein

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Gene

DUT

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP, preventing uracil incorporation into DNA.1 Publication

    Catalytic activityi

    dUTP + H2O = dUMP + diphosphate.

    Cofactori

    Binds 1 magnesium per trimer.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi138 – 1381Magnesium; shared with trimeric partners1 Publication

    GO - Molecular functioni

    1. dUTP diphosphatase activity Source: UniProtKB-EC
    2. identical protein binding Source: UniProtKB
    3. magnesium ion binding Source: UniProtKB

    GO - Biological processi

    1. DNA repair Source: TAIR
    2. dUMP biosynthetic process Source: UniProtKB-UniPathway
    3. dUTP metabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Nucleotide metabolism

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT3G46940-MONOMER.
    MetaCyc:AT3G46940-MONOMER.
    UniPathwayiUPA00610; UER00666.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deoxyuridine 5'-triphosphate nucleotidohydrolase (EC:3.6.1.23)
    Short name:
    dUTPase
    Alternative name(s):
    dUTP pyrophosphatase
    dUTP-pyrophosphatase-like 1
    Short name:
    AtDUT1
    Gene namesi
    Name:DUT
    Synonyms:DUT1
    Ordered Locus Names:At3g46940
    ORF Names:T6H20.30
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G46940.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: TAIR

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 166166Deoxyuridine 5'-triphosphate nucleotidohydrolasePRO_0000401366Add
    BLAST

    Proteomic databases

    PaxDbiQ9STG6.
    PRIDEiQ9STG6.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9STG6.

    Interactioni

    Subunit structurei

    Homotrimer.1 Publication

    Protein-protein interaction databases

    STRINGi3702.AT3G46940.1-P.

    Structurei

    Secondary structure

    1
    166
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi30 – 356
    Beta strandi51 – 544
    Beta strandi59 – 613
    Beta strandi66 – 7611
    Beta strandi81 – 866
    Helixi89 – 957
    Beta strandi97 – 1004
    Beta strandi103 – 1053
    Beta strandi112 – 1187
    Beta strandi120 – 1223
    Beta strandi124 – 1263
    Beta strandi131 – 14111
    Beta strandi145 – 1473

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2P9OX-ray2.00A/B/C1-166[»]
    2PC5X-ray2.20A/B/C1-166[»]
    ProteinModelPortaliQ9STG6.
    SMRiQ9STG6. Positions 26-153.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9STG6.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the dUTPase family.Curated

    Phylogenomic databases

    eggNOGiCOG0756.
    HOGENOMiHOG000028966.
    InParanoidiQ9STG6.
    KOiK01520.
    OMAiFERFDRI.
    PhylomeDBiQ9STG6.

    Family and domain databases

    Gene3Di2.70.40.10. 1 hit.
    InterProiIPR029054. dUTPase-like.
    IPR008180. dUTPase/dCTP_deaminase.
    IPR008181. dUTPase_1.
    [Graphical view]
    PfamiPF00692. dUTPase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51283. SSF51283. 1 hit.
    TIGRFAMsiTIGR00576. dut. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9STG6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MACVNEPSPK LQKLDRNGIH GDSSPSPFFK VKKLSEKAVI PTRGSPLSAG    50
    YDLSSAVDSK VPARGKALIP TDLSIAVPEG TYARIAPRSG LAWKHSIDVG 100
    AGVIDADYRG PVGVILFNHS DADFEVKFGD RIAQLIIEKI VTPDVVEVDD 150
    LDETVRGDGG FGSTGV 166
    Length:166
    Mass (Da):17,557
    Last modified:May 1, 2000 - v1
    Checksum:i5487738DF4A55BFF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL096859 Genomic DNA. Translation: CAB51171.1.
    CP002686 Genomic DNA. Translation: AEE78222.1.
    AF370334 mRNA. Translation: AAK44149.1.
    AY062989 mRNA. Translation: AAL34163.1.
    PIRiT12954.
    RefSeqiNP_190278.1. NM_114561.3.
    UniGeneiAt.48742.
    At.71138.

    Genome annotation databases

    EnsemblPlantsiAT3G46940.1; AT3G46940.1; AT3G46940.
    GeneIDi823847.
    KEGGiath:AT3G46940.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL096859 Genomic DNA. Translation: CAB51171.1 .
    CP002686 Genomic DNA. Translation: AEE78222.1 .
    AF370334 mRNA. Translation: AAK44149.1 .
    AY062989 mRNA. Translation: AAL34163.1 .
    PIRi T12954.
    RefSeqi NP_190278.1. NM_114561.3.
    UniGenei At.48742.
    At.71138.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2P9O X-ray 2.00 A/B/C 1-166 [» ]
    2PC5 X-ray 2.20 A/B/C 1-166 [» ]
    ProteinModelPortali Q9STG6.
    SMRi Q9STG6. Positions 26-153.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT3G46940.1-P.

    Proteomic databases

    PaxDbi Q9STG6.
    PRIDEi Q9STG6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G46940.1 ; AT3G46940.1 ; AT3G46940 .
    GeneIDi 823847.
    KEGGi ath:AT3G46940.

    Organism-specific databases

    TAIRi AT3G46940.

    Phylogenomic databases

    eggNOGi COG0756.
    HOGENOMi HOG000028966.
    InParanoidi Q9STG6.
    KOi K01520.
    OMAi FERFDRI.
    PhylomeDBi Q9STG6.

    Enzyme and pathway databases

    UniPathwayi UPA00610 ; UER00666 .
    BioCyci ARA:AT3G46940-MONOMER.
    MetaCyc:AT3G46940-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q9STG6.
    PROi Q9STG6.

    Gene expression databases

    Genevestigatori Q9STG6.

    Family and domain databases

    Gene3Di 2.70.40.10. 1 hit.
    InterProi IPR029054. dUTPase-like.
    IPR008180. dUTPase/dCTP_deaminase.
    IPR008181. dUTPase_1.
    [Graphical view ]
    Pfami PF00692. dUTPase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51283. SSF51283. 1 hit.
    TIGRFAMsi TIGR00576. dut. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "The SOS screen in Arabidopsis: a search for functions involved in DNA metabolism."
      Siaud N., Dubois E., Massot S., Richaud A., Dray E., Collier J., Doutriaux M.P.
      DNA Repair 9:567-578(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "Purification, crystallization and preliminary crystallographic analysis of deoxyuridine triphosphate nucleotidohydrolase from Arabidopsis thaliana."
      Bajaj M., Moriyama H.
      Acta Crystallogr. F 63:409-411(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) IN COMPLEX WITH MAGNESIUM, SUBUNIT.
    6. "Structure of dutpase from Arabidopsis thaliana."
      Bajaj M., Moriyama H.
      Submitted (FEB-2009) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS).

    Entry informationi

    Entry nameiDUT_ARATH
    AccessioniPrimary (citable) accession number: Q9STG6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 30, 2010
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Silencing of DUT leads to high seedling mortality and affects plant growth and flower organ morphology in surviving plants.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3