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Q9STG6

- DUT_ARATH

UniProt

Q9STG6 - DUT_ARATH

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Protein
Deoxyuridine 5'-triphosphate nucleotidohydrolase
Gene
DUT, DUT1, At3g46940, T6H20.30
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP, preventing uracil incorporation into DNA.1 Publication

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.

Cofactori

Binds 1 magnesium per trimer.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi138 – 1381Magnesium; shared with trimeric partners

GO - Molecular functioni

  1. dUTP diphosphatase activity Source: UniProtKB-EC
  2. identical protein binding Source: UniProtKB
  3. magnesium ion binding Source: UniProtKB

GO - Biological processi

  1. DNA repair Source: TAIR
  2. dUMP biosynthetic process Source: UniProtKB-UniPathway
  3. dUTP metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT3G46940-MONOMER.
MetaCyc:AT3G46940-MONOMER.
UniPathwayiUPA00610; UER00666.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase (EC:3.6.1.23)
Short name:
dUTPase
Alternative name(s):
dUTP pyrophosphatase
dUTP-pyrophosphatase-like 1
Short name:
AtDUT1
Gene namesi
Name:DUT
Synonyms:DUT1
Ordered Locus Names:At3g46940
ORF Names:T6H20.30
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G46940.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 166166Deoxyuridine 5'-triphosphate nucleotidohydrolase
PRO_0000401366Add
BLAST

Proteomic databases

PaxDbiQ9STG6.
PRIDEiQ9STG6.

Expressioni

Gene expression databases

GenevestigatoriQ9STG6.

Interactioni

Subunit structurei

Homotrimer.1 Publication

Protein-protein interaction databases

STRINGi3702.AT3G46940.1-P.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi30 – 356
Beta strandi51 – 544
Beta strandi59 – 613
Beta strandi66 – 7611
Beta strandi81 – 866
Helixi89 – 957
Beta strandi97 – 1004
Beta strandi103 – 1053
Beta strandi112 – 1187
Beta strandi120 – 1223
Beta strandi124 – 1263
Beta strandi131 – 14111
Beta strandi145 – 1473

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2P9OX-ray2.00A/B/C1-166[»]
2PC5X-ray2.20A/B/C1-166[»]
ProteinModelPortaliQ9STG6.
SMRiQ9STG6. Positions 26-153.

Miscellaneous databases

EvolutionaryTraceiQ9STG6.

Family & Domainsi

Sequence similaritiesi

Belongs to the dUTPase family.

Phylogenomic databases

eggNOGiCOG0756.
HOGENOMiHOG000028966.
InParanoidiQ9STG6.
KOiK01520.
OMAiFERFDRI.
PhylomeDBiQ9STG6.

Family and domain databases

Gene3Di2.70.40.10. 1 hit.
InterProiIPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR008181. dUTPase_1.
[Graphical view]
PfamiPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMiSSF51283. SSF51283. 1 hit.
TIGRFAMsiTIGR00576. dut. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9STG6-1 [UniParc]FASTAAdd to Basket

« Hide

MACVNEPSPK LQKLDRNGIH GDSSPSPFFK VKKLSEKAVI PTRGSPLSAG    50
YDLSSAVDSK VPARGKALIP TDLSIAVPEG TYARIAPRSG LAWKHSIDVG 100
AGVIDADYRG PVGVILFNHS DADFEVKFGD RIAQLIIEKI VTPDVVEVDD 150
LDETVRGDGG FGSTGV 166
Length:166
Mass (Da):17,557
Last modified:May 1, 2000 - v1
Checksum:i5487738DF4A55BFF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL096859 Genomic DNA. Translation: CAB51171.1.
CP002686 Genomic DNA. Translation: AEE78222.1.
AF370334 mRNA. Translation: AAK44149.1.
AY062989 mRNA. Translation: AAL34163.1.
PIRiT12954.
RefSeqiNP_190278.1. NM_114561.3.
UniGeneiAt.48742.
At.71138.

Genome annotation databases

EnsemblPlantsiAT3G46940.1; AT3G46940.1; AT3G46940.
GeneIDi823847.
KEGGiath:AT3G46940.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL096859 Genomic DNA. Translation: CAB51171.1 .
CP002686 Genomic DNA. Translation: AEE78222.1 .
AF370334 mRNA. Translation: AAK44149.1 .
AY062989 mRNA. Translation: AAL34163.1 .
PIRi T12954.
RefSeqi NP_190278.1. NM_114561.3.
UniGenei At.48742.
At.71138.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2P9O X-ray 2.00 A/B/C 1-166 [» ]
2PC5 X-ray 2.20 A/B/C 1-166 [» ]
ProteinModelPortali Q9STG6.
SMRi Q9STG6. Positions 26-153.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT3G46940.1-P.

Proteomic databases

PaxDbi Q9STG6.
PRIDEi Q9STG6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G46940.1 ; AT3G46940.1 ; AT3G46940 .
GeneIDi 823847.
KEGGi ath:AT3G46940.

Organism-specific databases

TAIRi AT3G46940.

Phylogenomic databases

eggNOGi COG0756.
HOGENOMi HOG000028966.
InParanoidi Q9STG6.
KOi K01520.
OMAi FERFDRI.
PhylomeDBi Q9STG6.

Enzyme and pathway databases

UniPathwayi UPA00610 ; UER00666 .
BioCyci ARA:AT3G46940-MONOMER.
MetaCyc:AT3G46940-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q9STG6.
PROi Q9STG6.

Gene expression databases

Genevestigatori Q9STG6.

Family and domain databases

Gene3Di 2.70.40.10. 1 hit.
InterProi IPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR008181. dUTPase_1.
[Graphical view ]
Pfami PF00692. dUTPase. 1 hit.
[Graphical view ]
SUPFAMi SSF51283. SSF51283. 1 hit.
TIGRFAMsi TIGR00576. dut. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "The SOS screen in Arabidopsis: a search for functions involved in DNA metabolism."
    Siaud N., Dubois E., Massot S., Richaud A., Dray E., Collier J., Doutriaux M.P.
    DNA Repair 9:567-578(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Purification, crystallization and preliminary crystallographic analysis of deoxyuridine triphosphate nucleotidohydrolase from Arabidopsis thaliana."
    Bajaj M., Moriyama H.
    Acta Crystallogr. F 63:409-411(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) IN COMPLEX WITH MAGNESIUM, SUBUNIT.
  6. "Structure of dutpase from Arabidopsis thaliana."
    Bajaj M., Moriyama H.
    Submitted (FEB-2009) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS).

Entry informationi

Entry nameiDUT_ARATH
AccessioniPrimary (citable) accession number: Q9STG6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: May 1, 2000
Last modified: June 11, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Silencing of DUT leads to high seedling mortality and affects plant growth and flower organ morphology in surviving plants.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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