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Q9STE8 (TC753_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein TOC75-3, chloroplastic
Alternative name(s):
75 kDa translocon at the outer-envelope-membrane of chloroplasts 3
Short name=AtTOC75-III
Gene names
Name:TOC75-3
Synonyms:TOC75
Ordered Locus Names:At3g46740
ORF Names:T6H20.230
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length818 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential protein. Mediates the insertion of proteins targeted to the outer membrane of chloroplasts By similarity. Required for the import of protein precursors into chloroplasts. Forms the voltage-dependent preprotein translocation channels (hydrophilic beta barrel) of the TOC complex in the chloroplastic outer membrane. Ref.12

Subunit structure

Part of the TOC core complex that includes a protein for the specific recognition of transit peptides surrounded by a ring composed of four proteins forming translocation channels, and four to five GTP-binding proteins providing energy. This core complex can interact with components of the TIC complex to form a larger import complex. Chloroplastic protein precursors such as prSS (precursor of the RuBisCO small subunit) also interact with these complexes. The TOC complex contains a specific subset of polar lipids such as digalactosyldiacylglyceride (DGDG), phosphatidylcholine (PC) and phosphatidylglycerol (PG). TOC75-3 interacts with TOC34/OEP34, TOC159/TOC86, TOC132 and TOC120. Ref.11

Subcellular location

Plastid › chloroplast outer membrane; Multi-pass membrane protein Ref.6 Ref.8 Ref.9 Ref.11.

Tissue specificity

Mostly expressed in young and actively dividing photosynthetic tissues and, to a lower extent, in old leaves and roots. Particularly low levels in leaves after etiolation. Ref.12

Developmental stage

Expressed predominantly during the early stages of plant growth, peaking at three weeks after germination (at protein level). Ref.11

Induction

Up-regulated by CIA2 in leaves. Ref.5

Domain

Transmembrane regions consist mainly of membrane-spanning sided beta-sheets, which are not predicted by sequence analysis tools.

Disruption phenotype

Plants have an embryo development arrested at the two cells stage. Ref.12

Sequence similarities

Belongs to the TOC75 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 7979Chloroplast Potential
Transit peptide80 – 14061Chloroplast; outer membrane Potential
Chain141 – 818678Protein TOC75-3, chloroplastic
PRO_0000042821

Regions

Topological domain141 – 15212Chloroplast intermembrane Potential
Transmembrane153 – 1619Beta stranded; Potential
Topological domain162 – 1698Cytoplasmic Potential
Transmembrane170 – 1789Beta stranded; Potential
Topological domain179 – 23456Chloroplast intermembrane Potential
Transmembrane235 – 2439Beta stranded; Potential
Topological domain244 – 25613Cytoplasmic Potential
Transmembrane257 – 2637Beta stranded; Potential
Topological domain264 – 366103Chloroplast intermembrane Potential
Transmembrane367 – 3748Beta stranded; Potential
Topological domain375 – 41945Cytoplasmic Potential
Transmembrane420 – 4278Beta stranded; Potential
Topological domain428 – 4369Chloroplast intermembrane Potential
Transmembrane437 – 4459Beta stranded; Potential
Topological domain446 – 4516Cytoplasmic Potential
Transmembrane452 – 46110Beta stranded; Potential
Topological domain462 – 47312Chloroplast intermembrane Potential
Transmembrane474 – 4829Beta stranded; Potential
Topological domain483 – 50927Cytoplasmic Potential
Transmembrane510 – 5189Beta stranded; Potential
Topological domain519 – 56244Chloroplast intermembrane Potential
Transmembrane563 – 5708Beta stranded; Potential
Topological domain571 – 5788Cytoplasmic Potential
Transmembrane579 – 5868Beta stranded; Potential
Topological domain587 – 693107Chloroplast intermembrane Potential
Transmembrane694 – 7029Beta stranded; Potential
Topological domain703 – 71412Cytoplasmic Potential
Transmembrane715 – 7239Beta stranded; Potential
Topological domain724 – 78562Chloroplast intermembrane Potential
Transmembrane786 – 7927Beta stranded; Potential
Topological domain793 – 80614Cytoplasmic Potential
Transmembrane807 – 8148Beta stranded; Potential
Topological domain815 – 8184Chloroplast intermembrane Potential
Compositional bias102 – 13029Gly-rich

Sequences

Sequence LengthMass (Da)Tools
Q9STE8 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 332E04D308F370F6

FASTA81889,189
        10         20         30         40         50         60 
MAAFSVNGQL IPTATSSTAS TSLSSRRKFL SPSSSRLPRI STQSPRVPSI KCSKSLPNRD 

        70         80         90        100        110        120 
TETSSKDSLL KNLAKPLAVA SVSSAASFFL FRISNLPSVL TGGGGGGDGN FGGFGGGGGG 

       130        140        150        160        170        180 
GDGNDGGFWG KLFSPSPAVA DEEQSPDWDS HGLPANIVVQ LNKLSGFKKY KVSDIMFFDR 

       190        200        210        220        230        240 
RRQTTIGTED SFFEMVSIRP GGVYTKAQLQ KELETLATCG MFEKVDLEGK TKPDGTLGVT 

       250        260        270        280        290        300 
ISFAESTWQS ADRFRCINVG LMVQSKPIEM DSDMTDKEKL EYYRSLEKDY KRRIDRARPC 

       310        320        330        340        350        360 
LLPAPVYGEV MQMLRDQGKV SARLLQRIRD RVQKWYHDEG YACAQVVNFG NLNTKEVVCE 

       370        380        390        400        410        420 
VVEGDITQLV IQFQDKLGNV VEGNTQVPVV RRELPKQLRQ GYVFNIEAGK KALSNINSLG 

       430        440        450        460        470        480 
LFSNIEVNPR PDEKNEGGII VEIKLKELEQ KSAEVSTEWS IVPGRGGAPT LASFQPGGSV 

       490        500        510        520        530        540 
TFEHRNLQGL NRSLMGSVTT SNFLNPQDDL SFKLEYVHPY LDGVYNPRNR TFKTSCFNSR 

       550        560        570        580        590        600 
KLSPVFTGGP GVEEVPPIWV DRAGVKANIT ENFTRQSKFT YGLVMEEITT RDESSHIAAN 

       610        620        630        640        650        660 
GQRLLPSGGI SADGPPTTLS GTGVDRMAFL QANITRDNTK FVNGAVVGQR TVFQVDQGLG 

       670        680        690        700        710        720 
IGSKFPFFNR HQLTMTKFIQ LREVEQGAGK SPPPVLVLHG HYGGCVGDLP SYDAFVLGGP 

       730        740        750        760        770        780 
YSVRGYNMGE LGAARNIAEV GAEIRIPVKN THVYAFVEHG NDLGSSKDVK GNPTAVYRRT 

       790        800        810 
GQGSSYGAGV KLGLVRAEYA VDHNNGTGAL FFRFGERY

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed: 11130713] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"A consensus nomenclature for the protein-import components of the chloroplast envelope."
Schnell D.J., Blobel G., Keegstra K., Kessler F., Ko K., Soll J.
Trends Cell Biol. 7:303-304(1997)
Cited for: NOMENCLATURE.
[5]"Leaf-specific upregulation of chloroplast translocon genes by a CCT motif-containing protein, CIA2."
Sun C.-W., Chen L.-J., Lin L.-C., Li H.-M.
Plant Cell 13:2053-2061(2001) [PubMed: 11549763] [Abstract]
Cited for: INDUCTION.
[6]"Integral membrane proteins of the chloroplast envelope: identification and subcellular localization of new transporters."
Ferro M., Salvi D., Riviere-Rolland H., Vermat T., Seigneurin-Berny D., Grunwald D., Garin J., Joyard J., Rolland N.
Proc. Natl. Acad. Sci. U.S.A. 99:11487-11492(2002) [PubMed: 12177442] [Abstract]
Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
[7]"Characterization of the translocon of the outer envelope of chloroplasts."
Schleiff E., Soll J., Kuechler M., Kuehlbrandt W., Harrer R.
J. Cell Biol. 160:541-551(2003) [PubMed: 12591914] [Abstract]
Cited for: TOC CORE COMPLEX COMPOSITION AND ARCHITECTURE.
[8]"Proteomic study of the Arabidopsis thaliana chloroplastic envelope membrane utilizing alternatives to traditional two-dimensional electrophoresis."
Froehlich J.E., Wilkerson C.G., Ray W.K., McAndrew R.S., Osteryoung K.W., Gage D.A., Phinney B.S.
J. Proteome Res. 2:413-425(2003) [PubMed: 12938931] [Abstract]
Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
[9]"Proteomics of the chloroplast envelope membranes from Arabidopsis thaliana."
Ferro M., Salvi D., Brugiere S., Miras S., Kowalski S., Louwagie M., Garin J., Joyard J., Rolland N.
Mol. Cell. Proteomics 2:325-345(2003) [PubMed: 12766230] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
Strain: cv. Wassilewskija.
[10]"A polyglycine stretch is necessary for proper targeting of the protein translocation channel precursor to the outer envelope membrane of chloroplasts."
Inoue K., Keegstra K.
Plant J. 34:661-669(2003) [PubMed: 12787247] [Abstract]
Cited for: PROTEOLYTIC PROCESSING.
[11]"Members of the Toc159 import receptor family represent distinct pathways for protein targeting to plastids."
Ivanova Y., Smith M.D., Chen K., Schnell D.J.
Mol. Biol. Cell 15:3379-3392(2004) [PubMed: 15090618] [Abstract]
Cited for: SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, INTERACTION WITH TOC132; TOC120 AND TOC159.
[12]"A molecular-genetic study of the Arabidopsis toc75 gene family."
Baldwin A., Wardle A., Patel R., Dudley P., Park S.K., Twell D., Inoue K., Jarvis P.
Plant Physiol. 138:715-733(2005) [PubMed: 15908591] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
Strain: cv. Columbia.
Tissue: Seedling.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AL096859 Genomic DNA. Translation: CAB51191.1.
CP002686 Genomic DNA. Translation: AEE78199.1.
AY127014 mRNA. Translation: AAM83239.1.
BT006358 mRNA. Translation: AAP21166.1.
IPIIPI00518793.
PIRT12975.
RefSeqNP_190258.1. NM_114541.2.
UniGeneAt.3633.
At.67270.

3D structure databases

ProteinModelPortalQ9STE8.
SMRQ9STE8. Positions 168-451.
ModBaseSearch...

Protein-protein interaction databases

IntActQ9STE8. 2 interactions.
STRINGQ9STE8.

Proteomic databases

PRIDEQ9STE8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G46740.1; AT3G46740.1; AT3G46740.
GeneID823827.
GenomeReviewsGene locus AT3G46740 in contig BA000014_GR.
KEGGath:AT3G46740.
NMPDRfig|3702.1.peg.15890.

Organism-specific databases

TAIRAt3g46740.

Phylogenomic databases

GeneTreeEPGT00070000029755.
HOGENOMHBG317894.
InParanoidQ9STE8.
OMARDESSHI.
PhylomeDBQ9STE8.
ProtClustDBPLN03138.

Gene expression databases

GenevestigatorQ9STE8.
GermOnlineAT3G46740. Arabidopsis thaliana.

Family and domain databases

InterProIPR000184. Bac_surfAg_D15.
IPR005689. IAP75.
IPR010827. Surface_Ag_variable_number.
[Graphical view]
PfamPF01103. Bac_surface_Ag. 1 hit.
PF07244. Surf_Ag_VNR. 1 hit.
[Graphical view]
TIGRFAMsTIGR00992. 3a0901s03IAP75. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTC753_ARATH
AccessionPrimary (citable) accession number: Q9STE8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: May 1, 2000
Last modified: December 14, 2011
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

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