Q9STD3 (CALR_CHLRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Calreticulin
Organism	Chlamydomonas reinhardtii (Chlamydomonas smithii)
Taxonomic identifier	3055 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Chlorophyta › Chlorophyceae › Chlamydomonadales › Chlamydomonadaceae › Chlamydomonas

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin may interact transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER By similarity.
Subcellular location	Endoplasmic reticulum lumen By similarity.
Domain	Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity By similarity. The interaction with glycans occurs through a binding site in the globular lectin domain By similarity. The zinc binding sites are localized to the N-domain By similarity.
Sequence similarities	Belongs to the calreticulin family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Repeat Signal
Ligand	Calcium Lectin Metal-binding Zinc
Molecular function	Chaperone
PTM	Disulfide bond
Gene Ontology (GO)
Biological_process	anthocyanin-containing compound metabolic process Inferred from electronic annotation. Source: EnsemblPlants/Gramene calcium ion homeostasis Inferred from electronic annotation. Source: EnsemblPlants/Gramene defense response signaling pathway, resistance gene-independent Inferred from electronic annotation. Source: EnsemblPlants/Gramene defense response to bacterium Inferred from electronic annotation. Source: EnsemblPlants/Gramene plant-type hypersensitive response Inferred from electronic annotation. Source: EnsemblPlants/Gramene protein folding Inferred from electronic annotation. Source: InterPro response to cadmium ion Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to oxidative stress Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to salt stress Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Cellular_component	chloroplast Inferred from electronic annotation. Source: EnsemblPlants/Gramene endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum membrane Inferred from electronic annotation. Source: EnsemblPlants/Gramene mitochondrion Inferred from electronic annotation. Source: EnsemblPlants/Gramene plasma membrane Inferred from electronic annotation. Source: EnsemblPlants/Gramene vacuole Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 420	402	Calreticulin		PRO_0000004189
Regions
	Repeat	194 – 205	12	1-1
	Repeat	213 – 224	12	1-2
	Repeat	230 – 241	12	1-3
	Repeat	248 – 259	12	1-4
	Repeat	263 – 273	11	2-1
	Repeat	277 – 287	11	2-2
	Repeat	291 – 301	11	2-3
	Region	194 – 259	66	4 X approximate repeats
	Region	263 – 301	39	3 X approximate repeats
	Motif	417 – 420	4	Prevents secretion from ER Potential
	Compositional bias	355 – 414	60	Asp/Glu/Lys-rich
Sites
	Binding site	110	1	Carbohydrate By similarity
	Binding site	112	1	Carbohydrate By similarity
	Binding site	131	1	Carbohydrate By similarity
	Binding site	138	1	Carbohydrate By similarity
	Binding site	321	1	Carbohydrate By similarity
Amino acid modifications
	Disulfide bond	106 ↔ 140		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9STD3 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: DD3BA3AFFBF61C9B	FASTA	420	47,328
	10 20 30 40 50 60 MKWGVVAVLA TLVVAASAKD YFKETFDGSW ADRWTKSSWK VSDGSAGEFK LTAGKWYGDA 70 80 90 100 110 120 EADKGIQTGP DSKFFAISAP LATVFDNTGK DTVVQFSVKH EQDLDCGGGY IKVVPATSEK 130 140 150 160 170 180 QMGEFGGDTP YSIMFGPDIC GYSTRKVHVI LTYKGKNYLI KKDIKAETDQ LTHVYTLVIK 190 200 210 220 230 240 PDNTYQVLID LKEVASGSLY EDWDMLPPKT IKDPKASKPE DWDEREEIAD PEDKKPEGWD 250 260 270 280 290 300 DIPATIADKD AKKPEDWDDE EDGTWEPPMI PNPEYKGEWK AKMIKNPAYK GIWVAPDIDN 310 320 330 340 350 360 PDYVHDDKLY NFKDLKFVGF ELWQVKSGSI FDNILVTDDL EAAKKFAEDT WGKHKDEEKA 370 380 390 400 410 420 MFDKVKKEED EKKAKDAPPP PVDAEAAEEE DDEYEDKEEP SGMGSIKIPK EEEESGHDEL

References

[1]	"Cloning and characterization of a cDNA encoding Chlamydomonas reinhardtii calreticulin." Zuppini A., Kaydamov C. Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: 137c / CC-125.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ000765 mRNA. Translation: CAB54526.1.
RefSeq	XP_001689661.1. XM_001689609.1.
UniGene	Cre.14231.
3D structure databases
ProteinModelPortal	Q9STD3.
SMR	Q9STD3. Positions 209-306.
ModBase	Search...
Proteomic databases
PRIDE	Q9STD3.
ProMEX	Q9STD3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5715514.
KEGG	cre:CHLREDRAFT_78954.
Phylogenomic databases
eggNOG	NOG305105.
KO	K08057.
OMA	WGVTKEP.
ProtClustDB	CLSN2920675.
Family and domain databases
Gene3D	2.60.120.200. 1 hit.
InterPro	IPR001580. Calret/calnex. IPR018124. Calret/calnex_CS. IPR009169. Calreticulin. IPR009033. Calreticulin/calnexin_P_dom. IPR008985. ConA-like_lec_gl_sf. IPR013320. ConA-like_subgrp. [Graphical view]
PANTHER	PTHR11073. PTHR11073. 1 hit.
Pfam	PF00262. Calreticulin. 1 hit. [Graphical view]
PIRSF	PIRSF002356. Calreticulin. 1 hit.
PRINTS	PR00626. CALRETICULIN.
SUPFAM	SSF63887. Calret_calnex_P. 1 hit. SSF49899. ConA_like_lec_gl. 2 hits.
PROSITE	PS00803. CALRETICULIN_1. 1 hit. PS00804. CALRETICULIN_2. 1 hit. PS00805. CALRETICULIN_REPEAT. 1 hit. PS00014. ER_TARGET. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CALR_CHLRE

Accession

Primary (citable) accession number: Q9STD3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	April 3, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program