Q9ST59 (RHT1_WHEAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DELLA protein RHT-1

Alternative name(s):
Protein Rht-B1/Rht-D1
Reduced height protein 1

Gene names

Name:

RHT1

Organism

Triticum aestivum (Wheat)

Taxonomic identifier

4565 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Triticum

Protein attributes

Sequence length	623 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Probably acts by participating in large multiprotein complexes that repress transcription of GA-inducible genes. Upon GA application, it is degraded by the proteasome, allowing the GA signaling pathway.
Subcellular location	Nucleus By similarity.
Domain	The DELLA motif is required for its GA-induced degradation Probable.
Post-translational modification	Phosphorylated By similarity. Ubiquitinated. Upon GA application it is ubiquitinated, leading to its subsequent degradation Probable.
Biotechnological use	This gene, also known as the 'green revolution gene' has been introduced by conventional breeding procedures into several cultivars. It produces shorter plants, increase grain yield at the expense of straw biomass, and are more resistant to damage by wind and rain. These wheats are short because they respond abnormally to GA.
Sequence similarities	Belongs to the GRAS family. DELLA subfamily.

Ontologies

Keywords
Biological process	Gibberellin signaling pathway Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Repressor
PTM	Phosphoprotein Ubl conjugation
Gene Ontology (GO)
Biological_process	gibberellic acid mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 623	623	DELLA protein RHT-1		PRO_0000132249
Regions
	Motif	38 – 42	5	DELLA motif
	Motif	338 – 342	5	VHIID
Natural variations
	Natural variant	61 – 64	4	Missing in strain: cv. Rht-D1b; induces a dwarf phenotype.
	Natural variant	62 – 64	3	Missing in strain: cv. Rht-B1b; induces a dwarf phenotype.

Sequences

	Sequence		Length	Mass (Da)
	Q9ST59 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: F2BAC34996D0A84F	FASTA	623	65,337
	10 20 30 40 50 60 MKREYQDAGG SGGGGGGMGS SEDKMMVSAA AGEGEEVDEL LAALGYKVRA SDMADVAQKL 70 80 90 100 110 120 EQLEMAMGMG GVGAGAAPDD SFATHLATDT VHYNPTDLSS WVESMLSELN APPPPLPPAP 130 140 150 160 170 180 QLNASTSSTV TGSGGYFDLP PSVDSSSSIY ALRPIPSPAG ATAPADLSAD SVRDPKRMRT 190 200 210 220 230 240 GGSSTSSSSS SSSSLGGGAR SSVVEAAPPV AAAANATPAL PVVVVDTQEA GIRLVHALLA 250 260 270 280 290 300 CAEAVQQENL SAAEALVKQI PLLAASQGGA MRKVAAYFGE ALARRVFRFR PQPDSSLLDA 310 320 330 340 350 360 AFADLLHAHF YESCPYLKFA HFTANQAILE AFAGCRRVHV VDFGIKQGMQ WPALLQALAL 370 380 390 400 410 420 RPGGPPSFRL TGVGPPQPDE TDALQQVGWK LAQFAHTIRV DFQYRGLVAA TLADLEPFML 430 440 450 460 470 480 QPEGEEDPNE EPEVIAVNSV FEMHRLLAQP GALEKVLGTV RAVRPRIVTV VEQEANHNSG 490 500 510 520 530 540 TFLDRFTESL HYYSTMFDSL EGGSSGGGPS EVSSGAAAAP AAAGTDQVMS EVYLGRQICN 550 560 570 580 590 600 VVACEGAERT ERHETLGQWR NRLGNAGFET VHLGSNAYKQ ASTLLALFAG GDGYKVEEKE 610 620 GCLTLGWHTR PLIATSAWRL AGP

References

[1]

"'Green revolution' genes encode mutant gibberellin response modulators." Peng J., Richards D.E., Hartley N.M., Murphy G.P., Devos K.M., Flintham J.E., Beales J., Fish L.J., Worland A.J., Pelica F., Sudhakar D., Christou P., Snape J.W., Gale M.D., Harberd N.P. Nature 400:256-261(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS GLN-61--GLU-64 DEL AND GLN-62--GLU-64 DEL. Strain: cv. Rht-B1b and cv. Rht-D1b.

Web resources

Protein Spotlight

All things dwarfed and beautiful - Issue 70 of May 2006

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ242531 Genomic DNA. Translation: CAB51555.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	Q9ST59.
Family and domain databases
InterPro	IPR021914. TF_DELLA_N. IPR005202. TF_GRAS. [Graphical view]
Pfam	PF12041. DELLA. 1 hit. PF03514. GRAS. 1 hit. [Graphical view]
PROSITE	PS50985. GRAS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RHT1_WHEAT

Accession

Primary (citable) accession number: Q9ST59

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	May 1, 2000
Last modified:	April 3, 2013
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program