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Q9ST48 (DWRF8_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DELLA protein DWARF8

Short name=Protein dwarf-8
Gene names
Name:D8
OrganismZea mays (Maize)
Taxonomic identifier4577 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

Protein attributes

Sequence length630 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Probably acts by participating in large multiprotein complexes that repress transcription of GA-inducible genes. Upon GA application, it is degraded by the proteasome, allowing the GA signaling pathway.

Subcellular location

Nucleus By similarity.

Domain

The DELLA motif is required for its GA-induced degradation Probable.

Post-translational modification

Phosphorylated By similarity.

Ubiquitinated. Upon GA application it is ubiquitinated, leading to its subsequent degradation Probable.

Polymorphism

Variations between cultivars affect flowering time and plant height.

Biotechnological use

This gene, also known as the 'green revolution gene' has been introduced by conventional breeding procedures into several cultivars. It produces shorter plants, increase grain yield at the expense of straw biomass, and are more resistant to damage by wind and rain.

Sequence similarities

Belongs to the GRAS family. DELLA subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 630630DELLA protein DWARF8
PRO_0000132246

Regions

Motif38 – 425DELLA motif
Motif347 – 3515VHIID
Motif451 – 4555LXXLL motif

Natural variations

Natural variant121G → D in strain: cv. CMV3.
Natural variant131G → GG in strain: cv. CML258, cv. CML5, cv. NC354 and cv. Q6199.
Natural variant281G → V in strain: cv. H99, cv. NC260, cv. PA91, cv. U267Y, cv. W64A and cv. WF9.
Natural variant55 – 595DVAQK → G in D8-d1; induces a dwarf phenotype.
Natural variant87 – 9812Missing in D8-2023; induces a dwarf phenotype.
Natural variant2311Missing in strain: cv. CM7.
Natural variant2551Q → H in strain: cv. MO24W.
Natural variant3041S → R in strain: cv. B103.
Natural variant3901T → I in strain: cv. EP1.
Natural variant4131Y → C in strain: cv. CMV3.
Natural variant4751I → T in strain: cv. GT112.
Natural variant4801E → K in strain: cv. H99.
Natural variant4841N → S in strain: cv. NC260.
Natural variant4861N → D in strain: cv. KUI43.
Natural variant5001Y → H in strain: cv. NC320.
Natural variant5051F → S in strain: cv. NC250.
Natural variant510 – 5112Missing in strain: cv. A619, cv. CM105, cv. CM174, cv. CMV3, cv. F7, cv. F2834T, cv. N192, cv. Ohio 43 and cv. P39.
Natural variant5191T → A in strain: cv. A619, cv. CM105, cv. CM7, cv. CM174, cv. CMV3, cv. F2834T, cv. F7, cv. N192, cv. Ohio 43 and cv. P39.
Natural variant5331M → K in strain: cv. F2.
Natural variant5781G → S in strain: cv. M37W and cv. Tzi10.

Sequences

Sequence LengthMass (Da)Tools
Q9ST48 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 3D56851726C51042

FASTA63066,029
        10         20         30         40         50         60 
MKREYQDAGG SGGDMGSSKD KMMAAAAGAG EQEEEDVDEL LAALGYKVRS SDMADVAQKL 

        70         80         90        100        110        120 
EQLEMAMGMG GVGGAGATAD DGFVSHLATD TVHYNPSDLS SWVESMLSEL NAPPAPLPPA 

       130        140        150        160        170        180 
TPAPRLASTS STVTSGAAAG AGYFDLPPAV DSSSSTYALK PIPSPVAAPS ADPSTDSARE 

       190        200        210        220        230        240 
PKRMRTGGGS TSSSSSSSSS MDGGRTRSSV VEAAPPATQA SAAANGPAVP VVVVDTQEAG 

       250        260        270        280        290        300 
IRLVHALLAC AEAVQQENFS AAEALVKQIP MLASSQGGAM RKVAAYFGEA LARRVYRFRP 

       310        320        330        340        350        360 
PPDSSLLDAA FADLLHAHFY ESCPYLKFAH FTANQAILEA FAGCRRVHVV DFGIKQGMQW 

       370        380        390        400        410        420 
PALLQALALR PGGPPSFRLT GVGPPQPDET DALQQVGWKL AQFAHTIRVD FQYRGLVAAT 

       430        440        450        460        470        480 
LADLEPFMLQ PEGDDTDDEP EVIAVNSVFE LHRLLAQPGA LEKVLGTVRA VRPRIVTVVE 

       490        500        510        520        530        540 
QEANHNSGTF LDRFTESLHY YSTMFDSLEG AGAGSGQSTD ASPAAAGGTD QVMSEVYLGR 

       550        560        570        580        590        600 
QICNVVACEG AERTERHETL GQWRSRLGGS GFAPVHLGSN AYKQASTLLA LFAGGDGYRV 

       610        620        630 
EEKDGCLTLG WHTRPLIATS AWRVAAAAAP 

« Hide

References

[1]"'Green revolution' genes encode mutant gibberellin response modulators."
Peng J., Richards D.E., Hartley N.M., Murphy G.P., Devos K.M., Flintham J.E., Beales J., Fish L.J., Worland A.J., Pelica F., Sudhakar D., Christou P., Snape J.W., Gale M.D., Harberd N.P.
Nature 400:256-261(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS D8-D1 55-ASP--LYS-59 DELINS GLY AND D8-2023 87-LEU--ASP-98 DEL.
[2]"Structure of linkage disequilibrium and phenotypic associations in the maize genome."
Remington D.L., Thornsberry J.M., Matsuoka Y., Wilson L.M., Whitt S.R., Doebley J., Kresovich S., Goodman M.M., Buckler E.S. IV
Proc. Natl. Acad. Sci. U.S.A. 98:11479-11484(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-581, VARIANTS.
Strain: cv. 38-11, cv. A272, cv. A619, cv. A632, cv. B103, cv. B104, cv. B37, cv. B68, cv. B73, cv. C103, cv. CM105, cv. CM174, cv. CML258, cv. CML281, cv. CML5, cv. CMV3, cv. EP1, cv. F2, cv. F2834T, cv. F7, cv. GT112, cv. H99, cv. I137TN, cv. I29, cv. KUI21, cv. KUI3, cv. KUI43, cv. M162W, cv. M37W, cv. Missouri 17, cv. Missouri 24W, cv. MS153, cv. N192, cv. ND246, cv. North Carolina 250, cv. North Carolina 260, cv. North Carolina 304, cv. North Carolina 320, cv. North Carolina 348, cv. North Carolina 350, cv. North Carolina 352, cv. North Carolina 354, cv. Ohio 43, cv. Ohio 7B, cv. P39, cv. PA91, cv. Q6199, cv. SA24, cv. SC213, cv. SG18, cv. Tennessee 232, cv. Texas 601, cv. Tzi10, cv. Tzi18, cv. U267Y, cv. WF9, cv. Wisconsin 117HT, cv. Wisconsin 182B and cv. Wisconsin 64A.
[3]"Patterns of DNA sequence polymorphism along chromosome 1 of maize (Zea mays ssp. mays L.)."
Tenaillon M.I., Sawkins M.C., Long A.D., Gaut R.L., Doebley J.F., Gaut B.S.
Proc. Natl. Acad. Sci. U.S.A. 98:9161-9166(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 169-434.
[4]"Maize full-length cDNA project."
Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., Haller K., Wing R., Soderlund C., Walbot V., Yu Y.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 477-630.
[5]"Dwarf8 polymorphisms associate with variation in flowering time."
Thornsberry J.M., Goodman M.M., Doebley J., Kresovich S., Nielsen D., Buckler E.S. IV
Nat. Genet. 28:286-289(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: POLYMORPHISM.
[6]"Validation of Dwarf8 polymorphisms associated with flowering time in elite European inbred lines of maize (Zea mays L.)."
Andersen J.R., Schrag T., Melchinger A.E., Zein I., Luebberstedt T.
Theor. Appl. Genet. 111:206-217(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: POLYMORPHISM.

Web resources

Protein Spotlight

All things dwarfed and beautiful - Issue 70 of May 2006

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ242530 Genomic DNA. Translation: CAB51557.1.
AF413112 Genomic DNA. Translation: AAL10302.1.
AF413113 Genomic DNA. Translation: AAL10303.1.
AF413114 Genomic DNA. Translation: AAL10304.1.
AF413115 Genomic DNA. Translation: AAL10305.1.
AF413116 Genomic DNA. Translation: AAL10306.1.
AF413117 Genomic DNA. Translation: AAL10307.1.
AF413118 Genomic DNA. Translation: AAL10308.1.
AF413119 Genomic DNA. Translation: AAL10309.1.
AF413120 Genomic DNA. Translation: AAL10310.1.
AF413121 Genomic DNA. Translation: AAL10311.1.
AF413122 Genomic DNA. Translation: AAL10312.1.
AF413123 Genomic DNA. Translation: AAL10313.1.
AF413124 Genomic DNA. Translation: AAL10314.1.
AF413125 Genomic DNA. Translation: AAL10315.1.
AF413126 Genomic DNA. Translation: AAL10316.1.
AF413127 Genomic DNA. Translation: AAL10317.1.
AF413128 Genomic DNA. Translation: AAL10318.1.
AF413129 Genomic DNA. Translation: AAL10319.1.
AF413130 Genomic DNA. Translation: AAL10320.1.
AF413131 Genomic DNA. Translation: AAL10321.1.
AF413132 Genomic DNA. Translation: AAL10322.1.
AF413133 Genomic DNA. Translation: AAL10323.1.
AF413134 Genomic DNA. Translation: AAL10324.1.
AF413135 Genomic DNA. Translation: AAL10325.1.
AF413136 Genomic DNA. Translation: AAL10326.1.
AF413137 Genomic DNA. Translation: AAL10327.1.
AF413138 Genomic DNA. Translation: AAL10328.1.
AF413139 Genomic DNA. Translation: AAL10329.1.
AF413140 Genomic DNA. Translation: AAL10330.1.
AF413141 Genomic DNA. Translation: AAL10331.1.
AF413142 Genomic DNA. Translation: AAL10332.1.
AF413143 Genomic DNA. Translation: AAL10333.1.
AF413144 Genomic DNA. Translation: AAL10334.1.
AF413145 Genomic DNA. Translation: AAL10335.1.
AF413146 Genomic DNA. Translation: AAL10336.1.
AF413147 Genomic DNA. Translation: AAL10337.1.
AF413148 Genomic DNA. Translation: AAL10338.1.
AF413149 Genomic DNA. Translation: AAL10339.1.
AF413150 Genomic DNA. Translation: AAL10340.1.
AF413151 Genomic DNA. Translation: AAL10341.1.
AF413152 Genomic DNA. Translation: AAL10342.1.
AF413153 Genomic DNA. Translation: AAL10343.1.
AF413154 Genomic DNA. Translation: AAL10344.1.
AF413155 Genomic DNA. Translation: AAL10345.1.
AF413156 Genomic DNA. Translation: AAL10346.1.
AF413157 Genomic DNA. Translation: AAL10347.1.
AF413158 Genomic DNA. Translation: AAL10348.1.
AF413159 Genomic DNA. Translation: AAL10349.1.
AF413160 Genomic DNA. Translation: AAL10350.1.
AF413161 Genomic DNA. Translation: AAL10351.1.
AF413162 Genomic DNA. Translation: AAL10352.1.
AF413163 Genomic DNA. Translation: AAL10353.1.
AF413164 Genomic DNA. Translation: AAL10354.1.
AF413165 Genomic DNA. Translation: AAL10355.1.
AF413166 Genomic DNA. Translation: AAL10356.1.
AF413167 Genomic DNA. Translation: AAL10357.1.
AF413168 Genomic DNA. Translation: AAL10358.1.
AF413169 Genomic DNA. Translation: AAL10359.1.
AF413170 Genomic DNA. Translation: AAL10360.1.
AF413171 Genomic DNA. Translation: AAL10361.1.
AF413172 Genomic DNA. Translation: AAL10362.1.
AF413173 Genomic DNA. Translation: AAL10363.1.
AF413174 Genomic DNA. Translation: AAL10364.1.
AF413175 Genomic DNA. Translation: AAL10365.1.
AF413176 Genomic DNA. Translation: AAL10366.1.
AF413177 Genomic DNA. Translation: AAL10367.1.
AF413178 Genomic DNA. Translation: AAL10368.1.
AF413179 Genomic DNA. Translation: AAL10369.1.
AF413180 Genomic DNA. Translation: AAL10370.1.
AF413181 Genomic DNA. Translation: AAL10371.1.
AF413182 Genomic DNA. Translation: AAL10372.1.
AF413183 Genomic DNA. Translation: AAL10373.1.
AF413184 Genomic DNA. Translation: AAL10374.1.
AF413185 Genomic DNA. Translation: AAL10375.1.
AF413186 Genomic DNA. Translation: AAL10376.1.
AF413187 Genomic DNA. Translation: AAL10377.1.
AF413188 Genomic DNA. Translation: AAL10378.1.
AF413189 Genomic DNA. Translation: AAL10379.1.
AF413190 Genomic DNA. Translation: AAL10380.1.
AF413191 Genomic DNA. Translation: AAL10381.1.
AF413192 Genomic DNA. Translation: AAL10382.1.
AF413193 Genomic DNA. Translation: AAL10383.1.
AF413194 Genomic DNA. Translation: AAL10384.1.
AF413195 Genomic DNA. Translation: AAL10385.1.
AF413196 Genomic DNA. Translation: AAL10386.1.
AF413197 Genomic DNA. Translation: AAL10387.1.
AF413198 Genomic DNA. Translation: AAL10388.1.
AF413199 Genomic DNA. Translation: AAL10389.1.
AF413200 Genomic DNA. Translation: AAL10390.1.
AF413201 Genomic DNA. Translation: AAL10391.1.
AF413202 Genomic DNA. Translation: AAL10392.1.
AF413203 Genomic DNA. Translation: AAL10393.1.
AF377621 Genomic DNA. Translation: AAK59901.1.
AF377622 Genomic DNA. Translation: AAK59902.1.
AF377623 Genomic DNA. Translation: AAK59903.1.
AF377624 Genomic DNA. Translation: AAK59904.1.
AF377625 Genomic DNA. Translation: AAK59905.1.
AF377626 Genomic DNA. Translation: AAK59906.1.
AF377627 Genomic DNA. Translation: AAK59907.1.
AF377628 Genomic DNA. Translation: AAK59908.1.
AF377629 Genomic DNA. Translation: AAK59909.1.
AF377630 Genomic DNA. Translation: AAK59910.1.
AF377631 Genomic DNA. Translation: AAK59911.1.
AF377632 Genomic DNA. Translation: AAK59912.1.
AF377633 Genomic DNA. Translation: AAK59913.1.
AF377634 Genomic DNA. Translation: AAK59914.1.
AF377635 Genomic DNA. Translation: AAK59915.1.
AF377636 Genomic DNA. Translation: AAK59916.1.
AF377637 Genomic DNA. Translation: AAK59917.1.
AF377638 Genomic DNA. Translation: AAK59918.1.
AF377639 Genomic DNA. Translation: AAK59919.1.
AF377640 Genomic DNA. Translation: AAK59920.1.
AF377641 Genomic DNA. Translation: AAK59921.1.
AF377642 Genomic DNA. Translation: AAK59922.1.
AF377643 Genomic DNA. Translation: AAK59923.1.
AF377644 Genomic DNA. Translation: AAK59924.1.
AF377645 Genomic DNA. Translation: AAK59925.1.
DR799058 mRNA. No translation available.
UniGeneZm.97982.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ9ST48.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneQ9ST48.
MaizeGDB12143.

Phylogenomic databases

OMARRVYRFR.

Family and domain databases

InterProIPR021914. TF_DELLA_N.
IPR005202. TF_GRAS.
[Graphical view]
PfamPF12041. DELLA. 1 hit.
PF03514. GRAS. 1 hit.
[Graphical view]
PROSITEPS50985. GRAS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDWRF8_MAIZE
AccessionPrimary (citable) accession number: Q9ST48
Secondary accession number(s): Q93V57 expand/collapse secondary AC list , Q93VT0, Q93VT1, Q93VT2, Q93WG1, Q93WI7, Q945I5, Q945I6, Q945I7, Q945I8, Q945I9, Q945J0, Q945J1, Q945J2, Q945J3, Q945J4, Q945J5, Q945J6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: May 1, 2000
Last modified: July 9, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries