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Protein

DELLA protein DWARF8

Gene

D8

Organism
Zea mays (Maize)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Probably acts by participating in large multiprotein complexes that repress transcription of GA-inducible genes. Upon GA application, it is degraded by the proteasome, allowing the GA signaling pathway.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRepressor
Biological processGibberellin signaling pathway, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
DELLA protein DWARF8
Short name:
Protein dwarf-8
Gene namesi
Name:D8
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogonodaeAndropogoneaeTripsacinaeZea
Proteomesi
  • UP000007305 Componenti: Chromosome 1

Organism-specific databases

MaizeGDBi12143

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Biotechnological usei

This gene, also known as the 'green revolution gene' has been introduced by conventional breeding procedures into several cultivars. It produces shorter plants, increase grain yield at the expense of straw biomass, and are more resistant to damage by wind and rain.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001322461 – 630DELLA protein DWARF8Add BLAST630

Post-translational modificationi

Phosphorylated.By similarity
Ubiquitinated. Upon GA application it is ubiquitinated, leading to its subsequent degradation (Probable).Curated

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9ST48
PRIDEiQ9ST48

Expressioni

Gene expression databases

ExpressionAtlasiQ9ST48 baseline and differential
GenevisibleiQ9ST48 ZM

Interactioni

Protein-protein interaction databases

STRINGi4577.GRMZM2G144744_P01

Structurei

3D structure databases

SMRiQ9ST48
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini234 – 623GRASPROSITE-ProRule annotationAdd BLAST390

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni241 – 297Leucine repeat I (LRI)PROSITE-ProRule annotationAdd BLAST57
Regioni316 – 381VHIIDPROSITE-ProRule annotationAdd BLAST66
Regioni395 – 427Leucine repeat II (LRII)PROSITE-ProRule annotationAdd BLAST33
Regioni443 – 544PFYREPROSITE-ProRule annotationAdd BLAST102
Regioni547 – 623SAWPROSITE-ProRule annotationAdd BLAST77

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi38 – 42DELLA motif5
Motifi248 – 252LxCxE motifPROSITE-ProRule annotation5
Motifi347 – 351VHIIDPROSITE-ProRule annotation5
Motifi451 – 455LXXLL motifPROSITE-ProRule annotation5

Domaini

The DELLA motif is required for its GA-induced degradation.Curated

Sequence similaritiesi

Belongs to the GRAS family. DELLA subfamily.Curated

Phylogenomic databases

eggNOGiENOG410IQYH Eukaryota
ENOG410YBFY LUCA
OMAiVQQENFA
OrthoDBiEOG0936075R

Family and domain databases

Gene3Di1.10.10.1290, 1 hit
InterProiView protein in InterPro
IPR038088 DELLA_N_sf
IPR030006 TF_DELLA
IPR021914 TF_DELLA_N
IPR005202 TF_GRAS
PANTHERiPTHR31636:SF47 PTHR31636:SF47, 1 hit
PfamiView protein in Pfam
PF12041 DELLA, 1 hit
PF03514 GRAS, 1 hit
PROSITEiView protein in PROSITE
PS50985 GRAS, 1 hit

Sequencei

Sequence statusi: Complete.

Q9ST48-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKREYQDAGG SGGDMGSSKD KMMAAAAGAG EQEEEDVDEL LAALGYKVRS
60 70 80 90 100
SDMADVAQKL EQLEMAMGMG GVGGAGATAD DGFVSHLATD TVHYNPSDLS
110 120 130 140 150
SWVESMLSEL NAPPAPLPPA TPAPRLASTS STVTSGAAAG AGYFDLPPAV
160 170 180 190 200
DSSSSTYALK PIPSPVAAPS ADPSTDSARE PKRMRTGGGS TSSSSSSSSS
210 220 230 240 250
MDGGRTRSSV VEAAPPATQA SAAANGPAVP VVVVDTQEAG IRLVHALLAC
260 270 280 290 300
AEAVQQENFS AAEALVKQIP MLASSQGGAM RKVAAYFGEA LARRVYRFRP
310 320 330 340 350
PPDSSLLDAA FADLLHAHFY ESCPYLKFAH FTANQAILEA FAGCRRVHVV
360 370 380 390 400
DFGIKQGMQW PALLQALALR PGGPPSFRLT GVGPPQPDET DALQQVGWKL
410 420 430 440 450
AQFAHTIRVD FQYRGLVAAT LADLEPFMLQ PEGDDTDDEP EVIAVNSVFE
460 470 480 490 500
LHRLLAQPGA LEKVLGTVRA VRPRIVTVVE QEANHNSGTF LDRFTESLHY
510 520 530 540 550
YSTMFDSLEG AGAGSGQSTD ASPAAAGGTD QVMSEVYLGR QICNVVACEG
560 570 580 590 600
AERTERHETL GQWRSRLGGS GFAPVHLGSN AYKQASTLLA LFAGGDGYRV
610 620 630
EEKDGCLTLG WHTRPLIATS AWRVAAAAAP
Length:630
Mass (Da):66,029
Last modified:May 1, 2000 - v1
Checksum:i3D56851726C51042
GO

Polymorphismi

Variations between cultivars affect flowering time and plant height.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti12G → D in strain: cv. CMV3. 1
Natural varianti13G → GG in strain: cv. CML258, cv. CML5, cv. NC354 and cv. Q6199. 1
Natural varianti28G → V in strain: cv. H99, cv. NC260, cv. PA91, cv. U267Y, cv. W64A and cv. WF9. 1
Natural varianti55 – 59DVAQK → G in D8-d1; induces a dwarf phenotype. 1 Publication5
Natural varianti87 – 98Missing in D8-2023; induces a dwarf phenotype. 1 PublicationAdd BLAST12
Natural varianti231Missing in strain: cv. CM7. 1
Natural varianti255Q → H in strain: cv. MO24W. 1
Natural varianti304S → R in strain: cv. B103. 1
Natural varianti390T → I in strain: cv. EP1. 1
Natural varianti413Y → C in strain: cv. CMV3. 1
Natural varianti475I → T in strain: cv. GT112. 1
Natural varianti480E → K in strain: cv. H99. 1
Natural varianti484N → S in strain: cv. NC260. 1
Natural varianti486N → D in strain: cv. KUI43. 1
Natural varianti500Y → H in strain: cv. NC320. 1
Natural varianti505F → S in strain: cv. NC250. 1
Natural varianti510 – 511Missing in strain: cv. A619, cv. CM105, cv. CM174, cv. CMV3, cv. F7, cv. F2834T, cv. N192, cv. Ohio 43 and cv. P39. 2
Natural varianti519T → A in strain: cv. A619, cv. CM105, cv. CM7, cv. CM174, cv. CMV3, cv. F2834T, cv. F7, cv. N192, cv. Ohio 43 and cv. P39. 1
Natural varianti533M → K in strain: cv. F2. 1
Natural varianti578G → S in strain: cv. M37W and cv. Tzi10. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ242530 Genomic DNA Translation: CAB51557.1
AF413112 Genomic DNA Translation: AAL10302.1
AF413113 Genomic DNA Translation: AAL10303.1
AF413114 Genomic DNA Translation: AAL10304.1
AF413115 Genomic DNA Translation: AAL10305.1
AF413116 Genomic DNA Translation: AAL10306.1
AF413117 Genomic DNA Translation: AAL10307.1
AF413118 Genomic DNA Translation: AAL10308.1
AF413119 Genomic DNA Translation: AAL10309.1
AF413120 Genomic DNA Translation: AAL10310.1
AF413121 Genomic DNA Translation: AAL10311.1
AF413122 Genomic DNA Translation: AAL10312.1
AF413123 Genomic DNA Translation: AAL10313.1
AF413124 Genomic DNA Translation: AAL10314.1
AF413125 Genomic DNA Translation: AAL10315.1
AF413126 Genomic DNA Translation: AAL10316.1
AF413127 Genomic DNA Translation: AAL10317.1
AF413128 Genomic DNA Translation: AAL10318.1
AF413129 Genomic DNA Translation: AAL10319.1
AF413130 Genomic DNA Translation: AAL10320.1
AF413131 Genomic DNA Translation: AAL10321.1
AF413132 Genomic DNA Translation: AAL10322.1
AF413133 Genomic DNA Translation: AAL10323.1
AF413134 Genomic DNA Translation: AAL10324.1
AF413135 Genomic DNA Translation: AAL10325.1
AF413136 Genomic DNA Translation: AAL10326.1
AF413137 Genomic DNA Translation: AAL10327.1
AF413138 Genomic DNA Translation: AAL10328.1
AF413139 Genomic DNA Translation: AAL10329.1
AF413140 Genomic DNA Translation: AAL10330.1
AF413141 Genomic DNA Translation: AAL10331.1
AF413142 Genomic DNA Translation: AAL10332.1
AF413143 Genomic DNA Translation: AAL10333.1
AF413144 Genomic DNA Translation: AAL10334.1
AF413145 Genomic DNA Translation: AAL10335.1
AF413146 Genomic DNA Translation: AAL10336.1
AF413147 Genomic DNA Translation: AAL10337.1
AF413148 Genomic DNA Translation: AAL10338.1
AF413149 Genomic DNA Translation: AAL10339.1
AF413150 Genomic DNA Translation: AAL10340.1
AF413151 Genomic DNA Translation: AAL10341.1
AF413152 Genomic DNA Translation: AAL10342.1
AF413153 Genomic DNA Translation: AAL10343.1
AF413154 Genomic DNA Translation: AAL10344.1
AF413155 Genomic DNA Translation: AAL10345.1
AF413156 Genomic DNA Translation: AAL10346.1
AF413157 Genomic DNA Translation: AAL10347.1
AF413158 Genomic DNA Translation: AAL10348.1
AF413159 Genomic DNA Translation: AAL10349.1
AF413160 Genomic DNA Translation: AAL10350.1
AF413161 Genomic DNA Translation: AAL10351.1
AF413162 Genomic DNA Translation: AAL10352.1
AF413163 Genomic DNA Translation: AAL10353.1
AF413164 Genomic DNA Translation: AAL10354.1
AF413165 Genomic DNA Translation: AAL10355.1
AF413166 Genomic DNA Translation: AAL10356.1
AF413167 Genomic DNA Translation: AAL10357.1
AF413168 Genomic DNA Translation: AAL10358.1
AF413169 Genomic DNA Translation: AAL10359.1
AF413170 Genomic DNA Translation: AAL10360.1
AF413171 Genomic DNA Translation: AAL10361.1
AF413172 Genomic DNA Translation: AAL10362.1
AF413173 Genomic DNA Translation: AAL10363.1
AF413174 Genomic DNA Translation: AAL10364.1
AF413175 Genomic DNA Translation: AAL10365.1
AF413176 Genomic DNA Translation: AAL10366.1
AF413177 Genomic DNA Translation: AAL10367.1
AF413178 Genomic DNA Translation: AAL10368.1
AF413179 Genomic DNA Translation: AAL10369.1
AF413180 Genomic DNA Translation: AAL10370.1
AF413181 Genomic DNA Translation: AAL10371.1
AF413182 Genomic DNA Translation: AAL10372.1
AF413183 Genomic DNA Translation: AAL10373.1
AF413184 Genomic DNA Translation: AAL10374.1
AF413185 Genomic DNA Translation: AAL10375.1
AF413186 Genomic DNA Translation: AAL10376.1
AF413187 Genomic DNA Translation: AAL10377.1
AF413188 Genomic DNA Translation: AAL10378.1
AF413189 Genomic DNA Translation: AAL10379.1
AF413190 Genomic DNA Translation: AAL10380.1
AF413191 Genomic DNA Translation: AAL10381.1
AF413192 Genomic DNA Translation: AAL10382.1
AF413193 Genomic DNA Translation: AAL10383.1
AF413194 Genomic DNA Translation: AAL10384.1
AF413195 Genomic DNA Translation: AAL10385.1
AF413196 Genomic DNA Translation: AAL10386.1
AF413197 Genomic DNA Translation: AAL10387.1
AF413198 Genomic DNA Translation: AAL10388.1
AF413199 Genomic DNA Translation: AAL10389.1
AF413200 Genomic DNA Translation: AAL10390.1
AF413201 Genomic DNA Translation: AAL10391.1
AF413202 Genomic DNA Translation: AAL10392.1
AF413203 Genomic DNA Translation: AAL10393.1
AF377621 Genomic DNA Translation: AAK59901.1
AF377622 Genomic DNA Translation: AAK59902.1
AF377623 Genomic DNA Translation: AAK59903.1
AF377624 Genomic DNA Translation: AAK59904.1
AF377625 Genomic DNA Translation: AAK59905.1
AF377626 Genomic DNA Translation: AAK59906.1
AF377627 Genomic DNA Translation: AAK59907.1
AF377628 Genomic DNA Translation: AAK59908.1
AF377629 Genomic DNA Translation: AAK59909.1
AF377630 Genomic DNA Translation: AAK59910.1
AF377631 Genomic DNA Translation: AAK59911.1
AF377632 Genomic DNA Translation: AAK59912.1
AF377633 Genomic DNA Translation: AAK59913.1
AF377634 Genomic DNA Translation: AAK59914.1
AF377635 Genomic DNA Translation: AAK59915.1
AF377636 Genomic DNA Translation: AAK59916.1
AF377637 Genomic DNA Translation: AAK59917.1
AF377638 Genomic DNA Translation: AAK59918.1
AF377639 Genomic DNA Translation: AAK59919.1
AF377640 Genomic DNA Translation: AAK59920.1
AF377641 Genomic DNA Translation: AAK59921.1
AF377642 Genomic DNA Translation: AAK59922.1
AF377643 Genomic DNA Translation: AAK59923.1
AF377644 Genomic DNA Translation: AAK59924.1
AF377645 Genomic DNA Translation: AAK59925.1
DR799058 mRNA No translation available.
UniGeneiZm.97982

Genome annotation databases

EnsemblPlantsiZm00001d033680_T001; Zm00001d033680_P001; Zm00001d033680
GrameneiZm00001d033680_T001; Zm00001d033680_P001; Zm00001d033680

Similar proteinsi

Entry informationi

Entry nameiDWRF8_MAIZE
AccessioniPrimary (citable) accession number: Q9ST48
Secondary accession number(s): Q93V57
, Q93VT0, Q93VT1, Q93VT2, Q93WG1, Q93WI7, Q945I5, Q945I6, Q945I7, Q945I8, Q945I9, Q945J0, Q945J1, Q945J2, Q945J3, Q945J4, Q945J5, Q945J6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: May 1, 2000
Last modified: March 28, 2018
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health