Q9ST02 (NAATA_HORVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nicotianamine aminotransferase A
EC=2.6.1.80

Alternative name(s):
Nicotianamine aminotransferase III
Short name=NAAT-III

Gene names

Name:

naat-A

Organism

Hordeum vulgare (Barley)

Taxonomic identifier

4513 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in biosynthesis of mugineic acid family phytosiderophores. Ref.1
Catalytic activity	Nicotianamine + 2-oxoglutarate = 3''-deamino-3''-oxonicotianamine + L-glutamate. Ref.1
Cofactor	Pyridoxal phosphate By similarity. UniProtKB P04694
Tissue specificity	Expressed in roots, but not in leaves. Ref.1
Induction	By iron deficiency. No expression is detected when sufficient iron is present. Ref.1
Sequence similarities	Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	biosynthetic process Inferred from electronic annotation. Source: InterPro cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	nicotianamine aminotransferase activity Inferred from direct assay Ref.1. Source: UniProtKB pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 461	461	Nicotianamine aminotransferase A		PRO_0000405350
Regions
	Compositional bias	11 – 130	120	Ala-rich
Amino acid modifications
	Modified residue	289	1	N6-(pyridoxal phosphate)lysine By similarity UniProtKB P04694
Experimental info
	Sequence conflict	402	1	D → N in BAA87055. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9ST02 [UniParc]. Last modified March 8, 2011. Version 2. Checksum: 85326EA8F38B4449	FASTA	461	49,565
	10 20 30 40 50 60 MVHQSNGHGE AAAAAANGKS NGHAAAANGK SNGHAAAAAV EWNFARGKDG ILATTGAKNS 70 80 90 100 110 120 IRAIRYKISA SVEESGPRPV LPLAHGDPSV FPAFRTAVEA EDAVAAALRT GQFNCYAAGV 130 140 150 160 170 180 GLPAARSAVA EHLSQGVPYK LSADDVFLTA GGTQAIEVII PVLAQTAGAN ILLPRPGYPN 190 200 210 220 230 240 YEARAAFNKL EVRHFDLIPD KGWEIDIDSL ESIADKNTTA MVIINPNNPC GSVYSYDHLA 250 260 270 280 290 300 KVAEVARKLG ILVIADEVYG KLVLGSAPFI PMGVFGHIAP VLSIGSLSKS WIVPGWRLGW 310 320 330 340 350 360 VAVYDPTKIL EKTKISTSIT NYLNVSTDPA TFVQEALPKI LENTKADFFK RIIGLLKESS 370 380 390 400 410 420 EICYREIKEN KYITCPHKPE GSMFVMVKLN LHLLEEIHDD IDFCCKLAKE ESVILCPGSV 430 440 450 460 LGMENWVRIT FACVPSSLQD GLERVKSFCQ RNKKKNSING C

References

[1]

"Cloning two genes for nicotianamine aminotransferase, a critical enzyme in iron acquisition (Strategy II) in graminaceous plants." Takahashi M., Yamaguchi H., Nakanishi H., Shioiri T., Nishizawa N.K., Mori S. Plant Physiol. 121:947-956(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], PROTEIN SEQUENCE OF 33-47; 222-232; 273-280 AND 385-403, FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY, INDUCTION. Strain: cv. Ehimehadaka No.1. Tissue: Root.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB024006 Genomic DNA. Translation: BAA87055.1. D88273 mRNA. Translation: BAA87052.2.
3D structure databases
ProteinModelPortal	Q9ST02.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
KEGG	dosa:Os02t0306401-00.
Organism-specific databases
Gramene	Q9ST02.
Enzyme and pathway databases
BRENDA	2.6.1.80. 2687.
Gene expression databases
Genevestigator	Q9ST02.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 1 hit.
InterPro	IPR004839. Aminotransferase_I/II. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. IPR005958. TyrNic_aminoTrfase. IPR021178. Tyrosine_transaminase. [Graphical view]
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
PIRSF	PIRSF000517. Tyr_transaminase. 1 hit.
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR01265. tyr_nico_aTase. 1 hit.
PROSITE	PS00105. AA_TRANSFER_CLASS_1. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAATA_HORVU

Accession

Primary (citable) accession number: Q9ST02
Secondary accession number(s): Q9SMG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 8, 2011
Last sequence update:	March 8, 2011
Last modified:	April 3, 2013
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program