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Protein

Zinc finger protein AZF2

Gene

AZF2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transcriptional repressor involved in the inhibition of plant growth under abiotic stress conditions. Can repress the expression of various genes, including osmotic stress and abscisic acid-repressive genes and auxin-inducible genes, by binding to their promoter regions in a DNA sequence-specific manner. Acts as a negative regulator of abscisic acid (ABA) signaling during seed germination. Probably involved in jasmonate (JA) early signaling response. May regulate the expression of the JA biosynthesis gene LOX3 and control the expression of TIFY10A/JAZ1, a key repressor in the JA signaling cascade. May act as a positive regulator of leaf senescence. Has been identified as a suppressor of the deficiency of yeast snf4 mutant to grow on non-fermentable carbon source.6 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri106 – 12823C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri165 – 18723C2H2-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: TAIR
  • metal ion binding Source: UniProtKB-KW
  • sequence-specific DNA binding Source: TAIR
  • transcription factor activity, sequence-specific DNA binding Source: TAIR
  • transcription regulatory region DNA binding Source: TAIR

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: UniProtKB-KW
  • embryo development ending in seed dormancy Source: TAIR
  • hyperosmotic salinity response Source: TAIR
  • negative regulation of transcription, DNA-templated Source: TAIR
  • response to abscisic acid Source: UniProtKB
  • response to chitin Source: TAIR
  • response to water deprivation Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Abscisic acid signaling pathway, Jasmonic acid signaling pathway, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein AZF2
Alternative name(s):
Zinc-finger protein 2
Gene namesi
Name:AZF2
Synonyms:ZF2
Ordered Locus Names:At3g19580
ORF Names:MMB12.27, MMB12.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G19580.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Delayed senescence and hypersensitivity to ABA during seed germination.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 273273Zinc finger protein AZF2PRO_0000421827Add
BLAST

Proteomic databases

PaxDbiQ9SSW2.
PRIDEiQ9SSW2.

Expressioni

Tissue specificityi

Expressed in roots, radicles, cotyledons, hypocotyls, leaf veins, stems, sepals, petals, stamens, placenta, funiculi and maturated seeds.3 Publications

Inductioni

By abscisic acid (ABA), ethylene, salt, cold, dehydration and the monovalent and divalent cations Li+, Na+, K+, Cs+, Ca2+ and Mg2+.4 Publications

Gene expression databases

GenevisibleiQ9SSW2. AT.

Interactioni

Protein-protein interaction databases

BioGridi6828. 1 interaction.
STRINGi3702.AT3G19580.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SSW2.
SMRiQ9SSW2. Positions 99-192.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi43 – 5210Poly-Ser
Compositional biasi193 – 1997Poly-Gly

Sequence similaritiesi

Contains 2 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri106 – 12823C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri165 – 18723C2H2-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
HOGENOMiHOG000240166.
OMAiVSEERSH.
PhylomeDBiQ9SSW2.

Family and domain databases

Gene3Di3.30.160.60. 1 hit.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF13912. zf-C2H2_6. 2 hits.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 2 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SSW2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALEAMNTPT SSFTRIETKE DLMNDAVFIE PWLKRKRSKR QRSHSPSSSS
60 70 80 90 100
SSPPRSRPKS QNQDLTEEEY LALCLLMLAK DQPSQTRFHQ QSQSLTPPPE
110 120 130 140 150
SKNLPYKCNV CEKAFPSYQA LGGHKASHRI KPPTVISTTA DDSTAPTISI
160 170 180 190 200
VAGEKHPIAA SGKIHECSIC HKVFPTGQAL GGHKRCHYEG NLGGGGGGGS
210 220 230 240 250
KSISHSGSVS STVSEERSHR GFIDLNLPAL PELSLHHNPI VDEEILSPLT
260 270
GKKPLLLTDH DQVIKKEDLS LKI
Length:273
Mass (Da):29,796
Last modified:May 1, 2000 - v1
Checksum:i010930062AAE70C9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB030730 Genomic DNA. Translation: BAA85107.1.
AF250337 mRNA. Translation: AAG10143.1.
AP000417 Genomic DNA. Translation: BAB02542.1.
CP002686 Genomic DNA. Translation: AEE76261.1.
CP002686 Genomic DNA. Translation: AEE76262.1.
BT003816 mRNA. Translation: AAO41869.1.
PIRiT52385.
RefSeqiNP_001118663.1. NM_001125191.1.
NP_188592.1. NM_112848.1.
UniGeneiAt.23023.

Genome annotation databases

EnsemblPlantsiAT3G19580.1; AT3G19580.1; AT3G19580.
AT3G19580.2; AT3G19580.2; AT3G19580.
GeneIDi821495.
GrameneiAT3G19580.1; AT3G19580.1; AT3G19580.
AT3G19580.2; AT3G19580.2; AT3G19580.
KEGGiath:AT3G19580.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB030730 Genomic DNA. Translation: BAA85107.1.
AF250337 mRNA. Translation: AAG10143.1.
AP000417 Genomic DNA. Translation: BAB02542.1.
CP002686 Genomic DNA. Translation: AEE76261.1.
CP002686 Genomic DNA. Translation: AEE76262.1.
BT003816 mRNA. Translation: AAO41869.1.
PIRiT52385.
RefSeqiNP_001118663.1. NM_001125191.1.
NP_188592.1. NM_112848.1.
UniGeneiAt.23023.

3D structure databases

ProteinModelPortaliQ9SSW2.
SMRiQ9SSW2. Positions 99-192.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi6828. 1 interaction.
STRINGi3702.AT3G19580.1.

Proteomic databases

PaxDbiQ9SSW2.
PRIDEiQ9SSW2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G19580.1; AT3G19580.1; AT3G19580.
AT3G19580.2; AT3G19580.2; AT3G19580.
GeneIDi821495.
GrameneiAT3G19580.1; AT3G19580.1; AT3G19580.
AT3G19580.2; AT3G19580.2; AT3G19580.
KEGGiath:AT3G19580.

Organism-specific databases

TAIRiAT3G19580.

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
HOGENOMiHOG000240166.
OMAiVSEERSH.
PhylomeDBiQ9SSW2.

Miscellaneous databases

PROiQ9SSW2.

Gene expression databases

GenevisibleiQ9SSW2. AT.

Family and domain databases

Gene3Di3.30.160.60. 1 hit.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF13912. zf-C2H2_6. 2 hits.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 2 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of a subset of the Arabidopsis Cys(2)/His(2)-type zinc-finger protein gene family under water stress."
    Sakamoto H., Araki T., Meshi T., Iwabuchi M.
    Gene 248:23-32(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, INDUCTION.
    Strain: cv. Columbia.
  2. "Functional identification of an Arabidopsis Snf4 ortholog by screening for heterologous multicopy suppressors of snf4 deficiency in yeast."
    Kleinow T., Bhalerao R., Breuer F., Umeda M., Salchert K., Koncz C.
    Plant J. 23:115-122(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    Strain: cv. Columbia.
  3. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
    DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Arabidopsis Cys2/His2-type zinc-finger proteins function as transcription repressors under drought, cold, and high-salinity stress conditions."
    Sakamoto H., Maruyama K., Sakuma Y., Meshi T., Iwabuchi M., Shinozaki K., Yamaguchi-Shinozaki K.
    Plant Physiol. 136:2734-2746(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
  7. "Mapping methyl jasmonate-mediated transcriptional reprogramming of metabolism and cell cycle progression in cultured Arabidopsis cells."
    Pauwels L., Morreel K., De Witte E., Lammertyn F., Van Montagu M., Boerjan W., Inze D., Goossens A.
    Proc. Natl. Acad. Sci. U.S.A. 105:1380-1385(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Arabidopsis zinc-finger protein 2 is a negative regulator of ABA signaling during seed germination."
    Drechsel G., Raab S., Hoth S.
    J. Plant Physiol. 167:1418-1421(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION, DISRUPTION PHENOTYPE.
  9. "Arabidopsis Cys2/His2 zinc-finger proteins AZF1 and AZF2 negatively regulate abscisic acid-repressive and auxin-inducible genes under abiotic stress conditions."
    Kodaira K.S., Qin F., Tran L.S., Maruyama K., Kidokoro S., Fujita Y., Shinozaki K., Yamaguchi-Shinozaki K.
    Plant Physiol. 157:742-756(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION.
  10. "Gene network analysis and functional studies of senescence-associated genes reveal novel regulators of Arabidopsis leaf senescence."
    Li Z., Peng J., Wen X., Guo H.
    J. Integr. Plant Biol. 54:526-539(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiAZF2_ARATH
AccessioniPrimary (citable) accession number: Q9SSW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 6, 2013
Last sequence update: May 1, 2000
Last modified: February 17, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Plants overexpressing AZF2 have increased sensitivity to salt stress and barely survive under high salt conditons.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.