ID   PSY_DAUCA               Reviewed;         398 AA.
AC   Q9SSU8;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   22-FEB-2023, entry version 89.
DE   RecName: Full=Phytoene synthase, chloroplastic;
DE            EC=2.5.1.32;
DE   Flags: Precursor;
GN   Name=PSY;
OS   Daucus carota (Wild carrot).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Apiales; Apiaceae; Apioideae; Scandiceae; Daucinae;
OC   Daucus; Daucus sect. Daucus.
OX   NCBI_TaxID=4039;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Root;
RA   Uno T., Sankawa U.;
RT   "Daucus carota phytoene synthase.";
RL   Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the reaction from prephytoene diphosphate to
CC       phytoene.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 (2E,6E,10E)-geranylgeranyl diphosphate = 15-cis-phytoene + 2
CC         diphosphate; Xref=Rhea:RHEA:34475, ChEBI:CHEBI:27787,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:58756; EC=2.5.1.32;
CC   -!- PATHWAY: Carotenoid biosynthesis; phytoene biosynthesis; all-trans-
CC       phytoene from geranylgeranyl diphosphate: step 1/1.
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the phytoene/squalene synthase family.
CC       {ECO:0000305}.
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DR   EMBL; AB032797; BAA84763.1; -; mRNA.
DR   AlphaFoldDB; Q9SSU8; -.
DR   SMR; Q9SSU8; -.
DR   EnsemblPlants; KZM89594; KZM89594; DCAR_023043.
DR   Gramene; KZM89594; KZM89594; DCAR_023043.
DR   OMA; KLYCYRV; -.
DR   UniPathway; UPA00799; UER00773.
DR   GO; GO:0010287; C:plastoglobule; IEA:EnsemblPlants.
DR   GO; GO:0046905; F:15-cis-phytoene synthase activity; IEA:EnsemblPlants.
DR   GO; GO:0004311; F:farnesyltranstransferase activity; IEA:InterPro.
DR   GO; GO:0016767; F:geranylgeranyl-diphosphate geranylgeranyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR   CDD; cd00683; Trans_IPPS_HH; 1.
DR   Gene3D; 1.10.600.10; Farnesyl Diphosphate Synthase; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR002060; Squ/phyt_synthse.
DR   InterPro; IPR019845; Squalene/phytoene_synthase_CS.
DR   InterPro; IPR044843; Trans_IPPS_bact-type.
DR   InterPro; IPR033904; Trans_IPPS_HH.
DR   PANTHER; PTHR31480; BIFUNCTIONAL LYCOPENE CYCLASE/PHYTOENE SYNTHASE; 1.
DR   PANTHER; PTHR31480:SF0; PHYTOENE SYNTHASE 2, CHLOROPLASTIC; 1.
DR   Pfam; PF00494; SQS_PSY; 1.
DR   SFLD; SFLDS00005; Isoprenoid_Synthase_Type_I; 1.
DR   SFLD; SFLDG01212; Phytoene_synthase_like; 1.
DR   SUPFAM; SSF48576; Terpenoid synthases; 1.
DR   PROSITE; PS01044; SQUALEN_PHYTOEN_SYN_1; 1.
DR   PROSITE; PS01045; SQUALEN_PHYTOEN_SYN_2; 1.
PE   2: Evidence at transcript level;
KW   Carotenoid biosynthesis; Chloroplast; Isoprene biosynthesis; Plastid;
KW   Transferase; Transit peptide.
FT   TRANSIT         1..?
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           ?..398
FT                   /note="Phytoene synthase, chloroplastic"
FT                   /id="PRO_0000029855"
SQ   SEQUENCE   398 AA;  45200 MW;  1D1E043824730615 CRC64;
     MACNFAVRVI YYPKEIHGVS VLNTNRSRKS RFSCRVMKLS TGVSAVAANP VRTSEERVYE
     VVLKQAALVR EEKRSSRGLC LDTKRTGSKS FDKSENDDAG MKSWNLLNEA YDRCGEVCAE
     YAKTFYLGTL LMTPERRRAV WAIYVWCRRT DELVDGPNAS HITPKALDRW EKRLNDLFDG
     QPYDMYDAAL ADTVSTYPVD IQPFKDMIDG MRMDLKKSRY QTFDELYLYC YYVAGTVGLM
     SVPVMGIAPE SKATTESVYS AALALGIANQ LTNILRDVGE DARRGRIYLP QEELKLAGIT
     PEYIFKGKVT DKWRSFMKGQ IKRARMFFDE AEKGVAELSS ASRWPVWASL LLYKQILDAI
     EANDYDNFTK RAYVGKAKKL VSLPLAYSRA LFAPSTVR
//