Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9SSQ9

- PLDA2_ARATH

UniProt

Q9SSQ9 - PLDA2_ARATH

Protein

Phospholipase D alpha 2

Gene

PLDALPHA2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond. Plays an important role in various cellular processes, including phytohormone action and response to stress, characterized by acidification of the cell.

    Catalytic activityi

    A phosphatidylcholine + H2O = choline + a phosphatidate.

    Cofactori

    Calcium. Calcium requirement for activity depends on pH. Active either under acidic conditions with micromolar levels of calcium (PIP2-dependent) or at neutral pH with millimolar levels of calcium (PIP2-independent).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei332 – 3321PROSITE-ProRule annotation
    Active sitei334 – 3341PROSITE-ProRule annotation
    Active sitei339 – 3391PROSITE-ProRule annotation
    Active sitei661 – 6611PROSITE-ProRule annotation
    Active sitei663 – 6631PROSITE-ProRule annotation
    Active sitei668 – 6681PROSITE-ProRule annotation

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. N-acylphosphatidylethanolamine-specific phospholipase D activity Source: UniProtKB-EC
    3. phospholipase D activity Source: UniProtKB-EC

    GO - Biological processi

    1. abscisic acid-activated signaling pathway Source: UniProtKB-KW
    2. ethylene-activated signaling pathway Source: UniProtKB-KW
    3. lipid catabolic process Source: UniProtKB-KW
    4. phosphatidylcholine metabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Abscisic acid signaling pathway, Ethylene signaling pathway, Lipid degradation, Lipid metabolism

    Keywords - Ligandi

    Calcium

    Enzyme and pathway databases

    BioCyciARA:AT1G52570-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phospholipase D alpha 2 (EC:3.1.4.4)
    Short name:
    AtPLDalpha2
    Short name:
    PLD alpha 2
    Alternative name(s):
    Choline phosphatase 2
    Phosphatidylcholine-hydrolyzing phospholipase D 2
    Gene namesi
    Name:PLDALPHA2
    Synonyms:PLD2
    Ordered Locus Names:At1g52570
    ORF Names:F6D8.21
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G52570.

    Subcellular locationi

    Cytoplasm 1 Publication. Membrane 1 Publication; Peripheral membrane protein 1 Publication. Vacuole 1 Publication. Cytoplasmic vesicleclathrin-coated vesicle 1 Publication
    Note: Found in vacuoles and also associated with plasma, microsomal and mitochondrial membranes and in clathrin-coated vesicles. Not found in chloroplast or nuclei. Activation increases association of preexisting enzyme with membranes. The distribution of this conventional PLD between membrane-associated and soluble fractions varied from organ to organ and is calcium-regulated.

    GO - Cellular componenti

    1. chloroplast envelope Source: TAIR
    2. clathrin-coated vesicle Source: UniProtKB-SubCell
    3. membrane Source: UniProtKB-SubCell
    4. vacuole Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Cytoplasmic vesicle, Membrane, Vacuole

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 810810Phospholipase D alpha 2PRO_0000218809Add
    BLAST

    Proteomic databases

    PaxDbiQ9SSQ9.
    PRIDEiQ9SSQ9.

    Expressioni

    Tissue specificityi

    Highly expressed in roots, stems and flowers, moderately in leaves, seedlings and siliques. Not detected in dry seeds.

    Inductioni

    Activated by abscisic acid (ABA), ethylene, heavy metal, cold, salt and osmotic stresses.

    Gene expression databases

    GenevestigatoriQ9SSQ9.

    Interactioni

    Protein-protein interaction databases

    BioGridi26914. 1 interaction.
    STRINGi3702.AT1G52570.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SSQ9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 110110C2Add
    BLAST
    Domaini327 – 36539PLD phosphodiesterase 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini656 – 68328PLD phosphodiesterase 2PROSITE-ProRule annotationAdd
    BLAST

    Domaini

    C2 domain is a calcium-binding fold, and the binding promotes the protein association with membranes. A lower affinity toward calcium can be anticipated for PLD alpha due to the absence of two potential calcium ligands.

    Sequence similaritiesi

    Contains 1 C2 domain.Curated
    Contains 2 PLD phosphodiesterase domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1502.
    HOGENOMiHOG000240112.
    InParanoidiQ9SSQ9.
    KOiK01115.
    OMAiRYPIGIA.
    PhylomeDBiQ9SSQ9.

    Family and domain databases

    Gene3Di2.60.40.150. 1 hit.
    InterProiIPR000008. C2_dom.
    IPR001736. PLipase_D/transphosphatidylase.
    IPR024632. PLipase_D_C.
    IPR015679. PLipase_D_fam.
    IPR011402. PLipase_D_pln.
    [Graphical view]
    PANTHERiPTHR18896. PTHR18896. 1 hit.
    PfamiPF00168. C2. 1 hit.
    PF12357. PLD_C. 1 hit.
    PF00614. PLDc. 2 hits.
    [Graphical view]
    PIRSFiPIRSF036470. PLD_plant. 1 hit.
    SMARTiSM00239. C2. 1 hit.
    SM00155. PLDc. 2 hits.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 1 hit.
    PROSITEiPS50035. PLD. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9SSQ9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEECLLHGRL HATIYEVDHL HAEGGRSGFL GSILANVEET IGVGKGETQL    50
    YATIDLEKAR VGRTRKITKE PKNPKWFESF HIYCGHMAKH VIFTVKDANP 100
    IGATLIGRGY IPVEDILHGE EVDRWVDILD NEKNPIAGGS KIHVKLQYFG 150
    VEKDKNWNRG IKSAKFPGVP YTFFSQRRGC KVSLYQDAHI PGNFVPKIPL 200
    AGGKNYEPHR CWEDIFDAIT NAKHLIYITG WSVYTEISLV RDSRRPKQGG 250
    DVTVGELLKK KASEGVKVIL LVWDDRTSVD LLKKDGLMAT HDEETENFFR 300
    GTDVNCILCP RNPDDGGSIV QNLQISTMFT HHQKIVVVDS EMPSGGSRSR 350
    RIVSFVGGLD LCDGRYDTPF HSLFRTLDTA HHDDFHQPNF TGAAITKGGP 400
    REPWHDIHCR LEGPIAWDVL YNFEQRWSRQ GGKDILVKMR ELGDIIIPPS 450
    PVLFSEDHDV WNVQLFRSID GGAAAGFPDS PEAAAEAGLV SGKDNIIDRS 500
    IQDAYIHAIR RAKDFIYIEN QYFLGSSFAW SADGIKPEEI NALHLIPKEL 550
    SLKIVSKIKA GEKFKVYVVV PMWPEGIPES GSVQAILDWQ KRTMEMMYKD 600
    VIKALRENGL EGEDPRDYLT FFCLGNREVK KDGEYEPSEK PEPDTDYIRA 650
    QEARRFMIYV HTKMMIVDDE YIIIGSANIN QRSMDGARDS EIAMGGYQPY 700
    HLSTRQPARG QIHGFRMSLW YEHLGMLDET FLDPSSQECI QKVNRVADKY 750
    WDLYSSESLE HDLPGHLLRY PIGIASEGNI TELPGCEFFP DTKARILGVK 800
    SDYMPPILTT 810
    Length:810
    Mass (Da):91,598
    Last modified:May 1, 2000 - v1
    Checksum:iF1FA36D8C9CFBCCA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC008016 Genomic DNA. Translation: AAD55607.1.
    CP002684 Genomic DNA. Translation: AEE32825.1.
    PIRiD96566.
    RefSeqiNP_175666.1. NM_104135.2.
    UniGeneiAt.52162.

    Genome annotation databases

    EnsemblPlantsiAT1G52570.1; AT1G52570.1; AT1G52570.
    GeneIDi841689.
    KEGGiath:AT1G52570.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC008016 Genomic DNA. Translation: AAD55607.1 .
    CP002684 Genomic DNA. Translation: AEE32825.1 .
    PIRi D96566.
    RefSeqi NP_175666.1. NM_104135.2.
    UniGenei At.52162.

    3D structure databases

    ProteinModelPortali Q9SSQ9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 26914. 1 interaction.
    STRINGi 3702.AT1G52570.1-P.

    Proteomic databases

    PaxDbi Q9SSQ9.
    PRIDEi Q9SSQ9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G52570.1 ; AT1G52570.1 ; AT1G52570 .
    GeneIDi 841689.
    KEGGi ath:AT1G52570.

    Organism-specific databases

    TAIRi AT1G52570.

    Phylogenomic databases

    eggNOGi COG1502.
    HOGENOMi HOG000240112.
    InParanoidi Q9SSQ9.
    KOi K01115.
    OMAi RYPIGIA.
    PhylomeDBi Q9SSQ9.

    Enzyme and pathway databases

    BioCyci ARA:AT1G52570-MONOMER.

    Gene expression databases

    Genevestigatori Q9SSQ9.

    Family and domain databases

    Gene3Di 2.60.40.150. 1 hit.
    InterProi IPR000008. C2_dom.
    IPR001736. PLipase_D/transphosphatidylase.
    IPR024632. PLipase_D_C.
    IPR015679. PLipase_D_fam.
    IPR011402. PLipase_D_pln.
    [Graphical view ]
    PANTHERi PTHR18896. PTHR18896. 1 hit.
    Pfami PF00168. C2. 1 hit.
    PF12357. PLD_C. 1 hit.
    PF00614. PLDc. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF036470. PLD_plant. 1 hit.
    SMARTi SM00239. C2. 1 hit.
    SM00155. PLDc. 2 hits.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 1 hit.
    PROSITEi PS50035. PLD. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Subcellular distribution and tissue expression of phospholipase Dalpha, Dbeta, and Dgamma in Arabidopsis."
      Fan L., Zheng S., Cui D., Wang X.
      Plant Physiol. 119:1371-1378(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiPLDA2_ARATH
    AccessioniPrimary (citable) accession number: Q9SSQ9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3