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Protein

Phospholipase D alpha 2

Gene

PLDALPHA2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond to generate phosphatidic acids (PA). Plays an important role in various cellular processes, including phytohormone action and response to stress, characterized by acidification of the cell.By similarity

Catalytic activityi

A phosphatidylcholine + H2O = choline + a phosphatidate.By similarity

Cofactori

Ca2+By similarityNote: Ca2+ requirement for activity depends on pH. Active either under acidic conditions with micromolar levels of calcium (PIP2-dependent) or at neutral pH with millimolar levels of calcium (PIP2-independent).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei332PROSITE-ProRule annotation1
Active sitei334PROSITE-ProRule annotation1
Active sitei339PROSITE-ProRule annotation1
Active sitei661PROSITE-ProRule annotation1
Active sitei663PROSITE-ProRule annotation1
Active sitei668PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processAbscisic acid signaling pathway, Ethylene signaling pathway, Lipid degradation, Lipid metabolism
LigandCalcium

Enzyme and pathway databases

BioCyciARA:AT1G52570-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase D alpha 21 Publication (EC:3.1.4.4By similarity)
Short name:
AtPLDalpha21 Publication
Short name:
PLD alpha 21 Publication
Alternative name(s):
Choline phosphatase 2
Phosphatidylcholine-hydrolyzing phospholipase D 2
Gene namesi
Name:PLDALPHA21 Publication
Synonyms:PLD2
Ordered Locus Names:At1g52570Imported
ORF Names:F6D8.21Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G52570
TAIRilocus:2035211 AT1G52570

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002188091 – 810Phospholipase D alpha 2Add BLAST810

Proteomic databases

PaxDbiQ9SSQ9
PRIDEiQ9SSQ9

PTM databases

iPTMnetiQ9SSQ9

Expressioni

Tissue specificityi

Highly expressed in roots, stems and flowers, moderately in leaves, seedlings and siliques. Not detected in dry seeds.1 Publication

Inductioni

Activated by abscisic acid (ABA), ethylene, heavy metal, cold, salt and osmotic stresses.

Gene expression databases

ExpressionAtlasiQ9SSQ9 baseline and differential
GenevisibleiQ9SSQ9 AT

Interactioni

Protein-protein interaction databases

BioGridi26914, 1 interactor
STRINGi3702.AT1G52570.1

Structurei

3D structure databases

ProteinModelPortaliQ9SSQ9
SMRiQ9SSQ9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 110C2Add BLAST110
Domaini327 – 365PLD phosphodiesterase 1PROSITE-ProRule annotationAdd BLAST39
Domaini656 – 683PLD phosphodiesterase 2PROSITE-ProRule annotationAdd BLAST28

Domaini

C2 domain is a calcium-binding fold, and the binding promotes the protein association with membranes. A lower affinity toward calcium can be anticipated for PLD alpha due to the absence of two potential calcium ligands.Curated

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1329 Eukaryota
COG1502 LUCA
HOGENOMiHOG000240112
InParanoidiQ9SSQ9
KOiK01115
OMAiGTKSDYM
OrthoDBiEOG093601XZ
PhylomeDBiQ9SSQ9

Family and domain databases

Gene3Di2.60.40.150, 1 hit
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR025202 PLD-like_dom
IPR001736 PLipase_D/transphosphatidylase
IPR024632 PLipase_D_C
IPR015679 PLipase_D_fam
IPR011402 PLipase_D_pln
PANTHERiPTHR18896 PTHR18896, 1 hit
PfamiView protein in Pfam
PF00168 C2, 1 hit
PF12357 PLD_C, 1 hit
PF00614 PLDc, 1 hit
PF13091 PLDc_2, 1 hit
PIRSFiPIRSF036470 PLD_plant, 1 hit
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00155 PLDc, 2 hits
PROSITEiView protein in PROSITE
PS50035 PLD, 2 hits

Sequencei

Sequence statusi: Complete.

Q9SSQ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEECLLHGRL HATIYEVDHL HAEGGRSGFL GSILANVEET IGVGKGETQL
60 70 80 90 100
YATIDLEKAR VGRTRKITKE PKNPKWFESF HIYCGHMAKH VIFTVKDANP
110 120 130 140 150
IGATLIGRGY IPVEDILHGE EVDRWVDILD NEKNPIAGGS KIHVKLQYFG
160 170 180 190 200
VEKDKNWNRG IKSAKFPGVP YTFFSQRRGC KVSLYQDAHI PGNFVPKIPL
210 220 230 240 250
AGGKNYEPHR CWEDIFDAIT NAKHLIYITG WSVYTEISLV RDSRRPKQGG
260 270 280 290 300
DVTVGELLKK KASEGVKVIL LVWDDRTSVD LLKKDGLMAT HDEETENFFR
310 320 330 340 350
GTDVNCILCP RNPDDGGSIV QNLQISTMFT HHQKIVVVDS EMPSGGSRSR
360 370 380 390 400
RIVSFVGGLD LCDGRYDTPF HSLFRTLDTA HHDDFHQPNF TGAAITKGGP
410 420 430 440 450
REPWHDIHCR LEGPIAWDVL YNFEQRWSRQ GGKDILVKMR ELGDIIIPPS
460 470 480 490 500
PVLFSEDHDV WNVQLFRSID GGAAAGFPDS PEAAAEAGLV SGKDNIIDRS
510 520 530 540 550
IQDAYIHAIR RAKDFIYIEN QYFLGSSFAW SADGIKPEEI NALHLIPKEL
560 570 580 590 600
SLKIVSKIKA GEKFKVYVVV PMWPEGIPES GSVQAILDWQ KRTMEMMYKD
610 620 630 640 650
VIKALRENGL EGEDPRDYLT FFCLGNREVK KDGEYEPSEK PEPDTDYIRA
660 670 680 690 700
QEARRFMIYV HTKMMIVDDE YIIIGSANIN QRSMDGARDS EIAMGGYQPY
710 720 730 740 750
HLSTRQPARG QIHGFRMSLW YEHLGMLDET FLDPSSQECI QKVNRVADKY
760 770 780 790 800
WDLYSSESLE HDLPGHLLRY PIGIASEGNI TELPGCEFFP DTKARILGVK
810
SDYMPPILTT
Length:810
Mass (Da):91,598
Last modified:May 1, 2000 - v1
Checksum:iF1FA36D8C9CFBCCA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC008016 Genomic DNA Translation: AAD55607.1
CP002684 Genomic DNA Translation: AEE32825.1
CP002684 Genomic DNA Translation: ANM60648.1
CP002684 Genomic DNA Translation: ANM60649.1
PIRiD96566
RefSeqiNP_001322920.1, NM_001333554.1
NP_001322921.1, NM_001333553.1
NP_175666.1, NM_104135.3

Genome annotation databases

EnsemblPlantsiAT1G52570.1; AT1G52570.1; AT1G52570
AT1G52570.2; AT1G52570.2; AT1G52570
AT1G52570.3; AT1G52570.3; AT1G52570
GeneIDi841689
GrameneiAT1G52570.1; AT1G52570.1; AT1G52570
AT1G52570.2; AT1G52570.2; AT1G52570
AT1G52570.3; AT1G52570.3; AT1G52570
KEGGiath:AT1G52570

Similar proteinsi

Entry informationi

Entry nameiPLDA2_ARATH
AccessioniPrimary (citable) accession number: Q9SSQ9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 1, 2000
Last modified: April 25, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health