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Protein

Phospholipase D alpha 2

Gene

PLDALPHA2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond to generate phosphatidic acids (PA). Plays an important role in various cellular processes, including phytohormone action and response to stress, characterized by acidification of the cell.By similarity

Catalytic activityi

A phosphatidylcholine + H2O = choline + a phosphatidate.By similarity

Cofactori

Ca2+By similarityNote: Ca2+ requirement for activity depends on pH. Active either under acidic conditions with micromolar levels of calcium (PIP2-dependent) or at neutral pH with millimolar levels of calcium (PIP2-independent).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei332PROSITE-ProRule annotation1
Active sitei334PROSITE-ProRule annotation1
Active sitei339PROSITE-ProRule annotation1
Active sitei661PROSITE-ProRule annotation1
Active sitei663PROSITE-ProRule annotation1
Active sitei668PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Abscisic acid signaling pathway, Ethylene signaling pathway, Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium

Enzyme and pathway databases

BioCyciARA:AT1G52570-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase D alpha 21 Publication (EC:3.1.4.4By similarity)
Short name:
AtPLDalpha21 Publication
Short name:
PLD alpha 21 Publication
Alternative name(s):
Choline phosphatase 2
Phosphatidylcholine-hydrolyzing phospholipase D 2
Gene namesi
Name:PLDALPHA21 Publication
Synonyms:PLD2
Ordered Locus Names:At1g52570Imported
ORF Names:F6D8.21Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G52570.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Membrane 1 Publication; Peripheral membrane protein 1 Publication
  • Vacuole 1 Publication
  • Cytoplasmic vesicleclathrin-coated vesicle 1 Publication

  • Note: Found in vacuoles and also associated with plasma, microsomal and mitochondrial membranes and in clathrin-coated vesicles. Not found in chloroplast or nuclei. Activation increases association of preexisting enzyme with membranes. The distribution of this conventional PLD between membrane-associated and soluble fractions varied from organ to organ and is calcium-regulated.

GO - Cellular componenti

  • chloroplast envelope Source: TAIR
  • clathrin-coated vesicle Source: UniProtKB-SubCell
  • membrane Source: UniProtKB-SubCell
  • vacuole Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002188091 – 810Phospholipase D alpha 2Add BLAST810

Proteomic databases

PaxDbiQ9SSQ9.
PRIDEiQ9SSQ9.

PTM databases

iPTMnetiQ9SSQ9.

Expressioni

Tissue specificityi

Highly expressed in roots, stems and flowers, moderately in leaves, seedlings and siliques. Not detected in dry seeds.1 Publication

Inductioni

Activated by abscisic acid (ABA), ethylene, heavy metal, cold, salt and osmotic stresses.

Gene expression databases

GenevisibleiQ9SSQ9. AT.

Interactioni

Protein-protein interaction databases

BioGridi26914. 1 interactor.
STRINGi3702.AT1G52570.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SSQ9.
SMRiQ9SSQ9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 110C2Add BLAST110
Domaini327 – 365PLD phosphodiesterase 1PROSITE-ProRule annotationAdd BLAST39
Domaini656 – 683PLD phosphodiesterase 2PROSITE-ProRule annotationAdd BLAST28

Domaini

C2 domain is a calcium-binding fold, and the binding promotes the protein association with membranes. A lower affinity toward calcium can be anticipated for PLD alpha due to the absence of two potential calcium ligands.Curated

Sequence similaritiesi

Contains 1 C2 domain.Curated
Contains 2 PLD phosphodiesterase domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1329. Eukaryota.
COG1502. LUCA.
HOGENOMiHOG000240112.
InParanoidiQ9SSQ9.
KOiK01115.
OMAiYPDDHET.
OrthoDBiEOG093601XZ.
PhylomeDBiQ9SSQ9.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR000008. C2_dom.
IPR025202. PLD-like_dom.
IPR001736. PLipase_D/transphosphatidylase.
IPR024632. PLipase_D_C.
IPR015679. PLipase_D_fam.
IPR011402. PLipase_D_pln.
[Graphical view]
PANTHERiPTHR18896. PTHR18896. 2 hits.
PfamiPF00168. C2. 1 hit.
PF12357. PLD_C. 1 hit.
PF00614. PLDc. 1 hit.
PF13091. PLDc_2. 1 hit.
[Graphical view]
PIRSFiPIRSF036470. PLD_plant. 1 hit.
SMARTiSM00239. C2. 1 hit.
SM00155. PLDc. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
PROSITEiPS50035. PLD. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SSQ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEECLLHGRL HATIYEVDHL HAEGGRSGFL GSILANVEET IGVGKGETQL
60 70 80 90 100
YATIDLEKAR VGRTRKITKE PKNPKWFESF HIYCGHMAKH VIFTVKDANP
110 120 130 140 150
IGATLIGRGY IPVEDILHGE EVDRWVDILD NEKNPIAGGS KIHVKLQYFG
160 170 180 190 200
VEKDKNWNRG IKSAKFPGVP YTFFSQRRGC KVSLYQDAHI PGNFVPKIPL
210 220 230 240 250
AGGKNYEPHR CWEDIFDAIT NAKHLIYITG WSVYTEISLV RDSRRPKQGG
260 270 280 290 300
DVTVGELLKK KASEGVKVIL LVWDDRTSVD LLKKDGLMAT HDEETENFFR
310 320 330 340 350
GTDVNCILCP RNPDDGGSIV QNLQISTMFT HHQKIVVVDS EMPSGGSRSR
360 370 380 390 400
RIVSFVGGLD LCDGRYDTPF HSLFRTLDTA HHDDFHQPNF TGAAITKGGP
410 420 430 440 450
REPWHDIHCR LEGPIAWDVL YNFEQRWSRQ GGKDILVKMR ELGDIIIPPS
460 470 480 490 500
PVLFSEDHDV WNVQLFRSID GGAAAGFPDS PEAAAEAGLV SGKDNIIDRS
510 520 530 540 550
IQDAYIHAIR RAKDFIYIEN QYFLGSSFAW SADGIKPEEI NALHLIPKEL
560 570 580 590 600
SLKIVSKIKA GEKFKVYVVV PMWPEGIPES GSVQAILDWQ KRTMEMMYKD
610 620 630 640 650
VIKALRENGL EGEDPRDYLT FFCLGNREVK KDGEYEPSEK PEPDTDYIRA
660 670 680 690 700
QEARRFMIYV HTKMMIVDDE YIIIGSANIN QRSMDGARDS EIAMGGYQPY
710 720 730 740 750
HLSTRQPARG QIHGFRMSLW YEHLGMLDET FLDPSSQECI QKVNRVADKY
760 770 780 790 800
WDLYSSESLE HDLPGHLLRY PIGIASEGNI TELPGCEFFP DTKARILGVK
810
SDYMPPILTT
Length:810
Mass (Da):91,598
Last modified:May 1, 2000 - v1
Checksum:iF1FA36D8C9CFBCCA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC008016 Genomic DNA. Translation: AAD55607.1.
CP002684 Genomic DNA. Translation: AEE32825.1.
PIRiD96566.
RefSeqiNP_175666.1. NM_104135.3.

Genome annotation databases

EnsemblPlantsiAT1G52570.1; AT1G52570.1; AT1G52570.
GeneIDi841689.
GrameneiAT1G52570.1; AT1G52570.1; AT1G52570.
KEGGiath:AT1G52570.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC008016 Genomic DNA. Translation: AAD55607.1.
CP002684 Genomic DNA. Translation: AEE32825.1.
PIRiD96566.
RefSeqiNP_175666.1. NM_104135.3.

3D structure databases

ProteinModelPortaliQ9SSQ9.
SMRiQ9SSQ9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi26914. 1 interactor.
STRINGi3702.AT1G52570.1.

PTM databases

iPTMnetiQ9SSQ9.

Proteomic databases

PaxDbiQ9SSQ9.
PRIDEiQ9SSQ9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G52570.1; AT1G52570.1; AT1G52570.
GeneIDi841689.
GrameneiAT1G52570.1; AT1G52570.1; AT1G52570.
KEGGiath:AT1G52570.

Organism-specific databases

TAIRiAT1G52570.

Phylogenomic databases

eggNOGiKOG1329. Eukaryota.
COG1502. LUCA.
HOGENOMiHOG000240112.
InParanoidiQ9SSQ9.
KOiK01115.
OMAiYPDDHET.
OrthoDBiEOG093601XZ.
PhylomeDBiQ9SSQ9.

Enzyme and pathway databases

BioCyciARA:AT1G52570-MONOMER.

Miscellaneous databases

PROiQ9SSQ9.

Gene expression databases

GenevisibleiQ9SSQ9. AT.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR000008. C2_dom.
IPR025202. PLD-like_dom.
IPR001736. PLipase_D/transphosphatidylase.
IPR024632. PLipase_D_C.
IPR015679. PLipase_D_fam.
IPR011402. PLipase_D_pln.
[Graphical view]
PANTHERiPTHR18896. PTHR18896. 2 hits.
PfamiPF00168. C2. 1 hit.
PF12357. PLD_C. 1 hit.
PF00614. PLDc. 1 hit.
PF13091. PLDc_2. 1 hit.
[Graphical view]
PIRSFiPIRSF036470. PLD_plant. 1 hit.
SMARTiSM00239. C2. 1 hit.
SM00155. PLDc. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
PROSITEiPS50035. PLD. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPLDA2_ARATH
AccessioniPrimary (citable) accession number: Q9SSQ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.