Reviewed, UniProtKB/Swiss-Prot Q9SSQ9 (PLDA2_ARATH)

Names and origin

Protein names

Recommended name:
    Phospholipase D alpha 2
      Short name=PLD alpha 2
      Short name=AtPLDalpha2
    EC=3.1.4.4
Alternative name(s):
    Choline phosphatase 2
    Phosphatidylcholine-hydrolyzing phospholipase D 2

Gene names

Name:	PLDALPHA2
Synonyms:	PLD2
Ordered Locus Names:	At1g52570
ORF Names:	F6D8.21

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	810 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond. Plays an important role in various cellular processes, including phytohormone action and response to stress, characterized by acidification of the cell.
Catalytic activity	A phosphatidylcholine + H2O = choline + a phosphatidate.
Cofactor	Calcium. Calcium requirement for activity depends on pH. Active either under acidic conditions with micromolar levels of calcium (PIP2-dependent) or at neutral pH with millimolar levels of calcium (PIP2-independent).
Subcellular location	Cytoplasm. Membrane; Peripheral membrane protein. Vacuole. Cytoplasmic vesicle › clathrin-coated vesicle. Note: Found in vacuoles and also associated with plasma, microsomal and mitochondrial membranes and in clathrin-coated vesicles. Not found in chloroplast or nuclei. Activation increases association of preexisting enzyme with membranes. The distribution of this conventional PLD between membrane-associated and soluble fractions varied from organ to organ and is calcium-regulated. Ref.2
Tissue specificity	Highly expressed in roots, stems and flowers, moderately in leaves, seedlings and siliques. Not detected in dry seeds.
Induction	Activated by abscisic acid (ABA), ethylene, heavy metal, cold, salt and osmotic stresses.
Domain	C2 domain is a calcium-binding fold, and the binding promotes the protein association with membranes. A lower affinity toward calcium can be anticipated for PLD alpha due to the absence of two potential calcium ligands.
Sequence similarities	Belongs to the phospholipase D family. C2-PLD subfamily. Contains 1 C2 domain. Contains 2 PLD phosphodiesterase domains.

Ontologies

Keywords
Biological process	Abscisic acid signaling pathway Ethylene signaling pathway Lipid degradation
Cellular component	Cytoplasm Cytoplasmic vesicle Membrane Vacuole
Domain	Repeat
Ligand	Calcium
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	abscisic acid mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW ethylene mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW phosphatidylcholine metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast envelope Inferred from direct assay. Source: TAIR clathrin-coated vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAPE-specific phospholipase D activity Inferred from electronic annotation. Source: EC calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipase D activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 810	810	Phospholipase D alpha 2		PRO_0000218809
Regions
	Domain	1 – 110	110	C2
	Domain	327 – 365	39	PLD phosphodiesterase 1
	Domain	656 – 683	28	PLD phosphodiesterase 2
Sites
	Active site	332	1	Potential
	Active site	334	1	Potential
	Active site	339	1	Potential
	Active site	661	1	Potential
	Active site	663	1	Potential
	Active site	668	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9SSQ9-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: F1FA36D8C9CFBCCA	FASTA	810	91,598
	10 20 30 40 50 60 MEECLLHGRL HATIYEVDHL HAEGGRSGFL GSILANVEET IGVGKGETQL YATIDLEKAR 70 80 90 100 110 120 VGRTRKITKE PKNPKWFESF HIYCGHMAKH VIFTVKDANP IGATLIGRGY IPVEDILHGE 130 140 150 160 170 180 EVDRWVDILD NEKNPIAGGS KIHVKLQYFG VEKDKNWNRG IKSAKFPGVP YTFFSQRRGC 190 200 210 220 230 240 KVSLYQDAHI PGNFVPKIPL AGGKNYEPHR CWEDIFDAIT NAKHLIYITG WSVYTEISLV 250 260 270 280 290 300 RDSRRPKQGG DVTVGELLKK KASEGVKVIL LVWDDRTSVD LLKKDGLMAT HDEETENFFR 310 320 330 340 350 360 GTDVNCILCP RNPDDGGSIV QNLQISTMFT HHQKIVVVDS EMPSGGSRSR RIVSFVGGLD 370 380 390 400 410 420 LCDGRYDTPF HSLFRTLDTA HHDDFHQPNF TGAAITKGGP REPWHDIHCR LEGPIAWDVL 430 440 450 460 470 480 YNFEQRWSRQ GGKDILVKMR ELGDIIIPPS PVLFSEDHDV WNVQLFRSID GGAAAGFPDS 490 500 510 520 530 540 PEAAAEAGLV SGKDNIIDRS IQDAYIHAIR RAKDFIYIEN QYFLGSSFAW SADGIKPEEI 550 560 570 580 590 600 NALHLIPKEL SLKIVSKIKA GEKFKVYVVV PMWPEGIPES GSVQAILDWQ KRTMEMMYKD 610 620 630 640 650 660 VIKALRENGL EGEDPRDYLT FFCLGNREVK KDGEYEPSEK PEPDTDYIRA QEARRFMIYV 670 680 690 700 710 720 HTKMMIVDDE YIIIGSANIN QRSMDGARDS EIAMGGYQPY HLSTRQPARG QIHGFRMSLW 730 740 750 760 770 780 YEHLGMLDET FLDPSSQECI QKVNRVADKY WDLYSSESLE HDLPGHLLRY PIGIASEGNI 790 800 810 TELPGCEFFP DTKARILGVK SDYMPPILTT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Subcellular distribution and tissue expression of phospholipase Dalpha, Dbeta, and Dgamma in Arabidopsis." Fan L., Zheng S., Cui D., Wang X. Plant Physiol. 119:1371-1378(1999) [PubMed: 10198096] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC008016 Genomic DNA. Translation: AAD55607.1.
IPI	IPI00542684.
PIR	D96566.
RefSeq	NP_175666.1.
UniGene	At.52162
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q9SSQ9.
Genome annotation databases
GeneID	841689.
GenomeReviews	Gene locus AT1G52570 in contig CT485782_GR.
KEGG	ath:AT1G52570.
NMPDR	fig|3702.1.peg.4742.
Organism-specific databases
TAIR	At1g52570.
Phylogenomic databases
OMA	DAITNAK.
Enzyme and pathway databases
BRENDA	3.1.4.4. 302.
Gene expression databases
ArrayExpress	Q9SSQ9.
Genevestigator	Q9SSQ9.
GermOnline	AT1G52570. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR015679. Phospholipase_D. IPR011402. PLD_pln. IPR001736. PLipase_D/transphosphatidylase. [Graphical view]
PANTHER	PTHR18896. Phospholipase_D. 1 hit.
Pfam	PF00168. C2. 1 hit. PF00614. PLDc. 2 hits. [Graphical view]
PIRSF	PIRSF036470. PLD_plant. 1 hit.
SMART	SM00239. C2. 1 hit. SM00155. PLDc. 2 hits. [Graphical view]
PROSITE	PS50004. C2. False negative. PS50035. PLD. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLDA2_ARATH

Accession

Primary (citable) accession number: Q9SSQ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	May 1, 2000
Last modified:	November 3, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)