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Q9SSQ9

- PLDA2_ARATH

UniProt

Q9SSQ9 - PLDA2_ARATH

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Protein
Phospholipase D alpha 2
Gene
PLDALPHA2, PLD2, At1g52570, F6D8.21
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond. Plays an important role in various cellular processes, including phytohormone action and response to stress, characterized by acidification of the cell.

Catalytic activityi

A phosphatidylcholine + H2O = choline + a phosphatidate.

Cofactori

Calcium. Calcium requirement for activity depends on pH. Active either under acidic conditions with micromolar levels of calcium (PIP2-dependent) or at neutral pH with millimolar levels of calcium (PIP2-independent).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei332 – 3321 Reviewed prediction
Active sitei334 – 3341 Reviewed prediction
Active sitei339 – 3391 Reviewed prediction
Active sitei661 – 6611 Reviewed prediction
Active sitei663 – 6631 Reviewed prediction
Active sitei668 – 6681 Reviewed prediction

GO - Molecular functioni

  1. N-acylphosphatidylethanolamine-specific phospholipase D activity Source: UniProtKB-EC
  2. calcium ion binding Source: InterPro
  3. phospholipase D activity Source: UniProtKB-EC

GO - Biological processi

  1. abscisic acid-activated signaling pathway Source: UniProtKB-KW
  2. ethylene-activated signaling pathway Source: UniProtKB-KW
  3. lipid catabolic process Source: UniProtKB-KW
  4. phosphatidylcholine metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Abscisic acid signaling pathway, Ethylene signaling pathway, Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium

Enzyme and pathway databases

BioCyciARA:AT1G52570-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase D alpha 2 (EC:3.1.4.4)
Short name:
AtPLDalpha2
Short name:
PLD alpha 2
Alternative name(s):
Choline phosphatase 2
Phosphatidylcholine-hydrolyzing phospholipase D 2
Gene namesi
Name:PLDALPHA2
Synonyms:PLD2
Ordered Locus Names:At1g52570
ORF Names:F6D8.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G52570.

Subcellular locationi

Cytoplasm. Membrane; Peripheral membrane protein. Vacuole. Cytoplasmic vesicleclathrin-coated vesicle
Note: Found in vacuoles and also associated with plasma, microsomal and mitochondrial membranes and in clathrin-coated vesicles. Not found in chloroplast or nuclei. Activation increases association of preexisting enzyme with membranes. The distribution of this conventional PLD between membrane-associated and soluble fractions varied from organ to organ and is calcium-regulated.1 Publication

GO - Cellular componenti

  1. chloroplast envelope Source: TAIR
  2. clathrin-coated vesicle Source: UniProtKB-SubCell
  3. membrane Source: UniProtKB-SubCell
  4. vacuole Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 810810Phospholipase D alpha 2
PRO_0000218809Add
BLAST

Proteomic databases

PaxDbiQ9SSQ9.
PRIDEiQ9SSQ9.

Expressioni

Tissue specificityi

Highly expressed in roots, stems and flowers, moderately in leaves, seedlings and siliques. Not detected in dry seeds.

Inductioni

Activated by abscisic acid (ABA), ethylene, heavy metal, cold, salt and osmotic stresses.

Gene expression databases

GenevestigatoriQ9SSQ9.

Interactioni

Protein-protein interaction databases

BioGridi26914. 1 interaction.
STRINGi3702.AT1G52570.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9SSQ9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 110110C2
Add
BLAST
Domaini327 – 36539PLD phosphodiesterase 1
Add
BLAST
Domaini656 – 68328PLD phosphodiesterase 2
Add
BLAST

Domaini

C2 domain is a calcium-binding fold, and the binding promotes the protein association with membranes. A lower affinity toward calcium can be anticipated for PLD alpha due to the absence of two potential calcium ligands.

Sequence similaritiesi

Contains 1 C2 domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1502.
HOGENOMiHOG000240112.
InParanoidiQ9SSQ9.
KOiK01115.
OMAiRYPIGIA.
PhylomeDBiQ9SSQ9.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR000008. C2_dom.
IPR001736. PLipase_D/transphosphatidylase.
IPR024632. PLipase_D_C.
IPR015679. PLipase_D_fam.
IPR011402. PLipase_D_pln.
[Graphical view]
PANTHERiPTHR18896. PTHR18896. 1 hit.
PfamiPF00168. C2. 1 hit.
PF12357. PLD_C. 1 hit.
PF00614. PLDc. 2 hits.
[Graphical view]
PIRSFiPIRSF036470. PLD_plant. 1 hit.
SMARTiSM00239. C2. 1 hit.
SM00155. PLDc. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
PROSITEiPS50035. PLD. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SSQ9-1 [UniParc]FASTAAdd to Basket

« Hide

MEECLLHGRL HATIYEVDHL HAEGGRSGFL GSILANVEET IGVGKGETQL    50
YATIDLEKAR VGRTRKITKE PKNPKWFESF HIYCGHMAKH VIFTVKDANP 100
IGATLIGRGY IPVEDILHGE EVDRWVDILD NEKNPIAGGS KIHVKLQYFG 150
VEKDKNWNRG IKSAKFPGVP YTFFSQRRGC KVSLYQDAHI PGNFVPKIPL 200
AGGKNYEPHR CWEDIFDAIT NAKHLIYITG WSVYTEISLV RDSRRPKQGG 250
DVTVGELLKK KASEGVKVIL LVWDDRTSVD LLKKDGLMAT HDEETENFFR 300
GTDVNCILCP RNPDDGGSIV QNLQISTMFT HHQKIVVVDS EMPSGGSRSR 350
RIVSFVGGLD LCDGRYDTPF HSLFRTLDTA HHDDFHQPNF TGAAITKGGP 400
REPWHDIHCR LEGPIAWDVL YNFEQRWSRQ GGKDILVKMR ELGDIIIPPS 450
PVLFSEDHDV WNVQLFRSID GGAAAGFPDS PEAAAEAGLV SGKDNIIDRS 500
IQDAYIHAIR RAKDFIYIEN QYFLGSSFAW SADGIKPEEI NALHLIPKEL 550
SLKIVSKIKA GEKFKVYVVV PMWPEGIPES GSVQAILDWQ KRTMEMMYKD 600
VIKALRENGL EGEDPRDYLT FFCLGNREVK KDGEYEPSEK PEPDTDYIRA 650
QEARRFMIYV HTKMMIVDDE YIIIGSANIN QRSMDGARDS EIAMGGYQPY 700
HLSTRQPARG QIHGFRMSLW YEHLGMLDET FLDPSSQECI QKVNRVADKY 750
WDLYSSESLE HDLPGHLLRY PIGIASEGNI TELPGCEFFP DTKARILGVK 800
SDYMPPILTT 810
Length:810
Mass (Da):91,598
Last modified:May 1, 2000 - v1
Checksum:iF1FA36D8C9CFBCCA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC008016 Genomic DNA. Translation: AAD55607.1.
CP002684 Genomic DNA. Translation: AEE32825.1.
PIRiD96566.
RefSeqiNP_175666.1. NM_104135.2.
UniGeneiAt.52162.

Genome annotation databases

EnsemblPlantsiAT1G52570.1; AT1G52570.1; AT1G52570.
GeneIDi841689.
KEGGiath:AT1G52570.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC008016 Genomic DNA. Translation: AAD55607.1 .
CP002684 Genomic DNA. Translation: AEE32825.1 .
PIRi D96566.
RefSeqi NP_175666.1. NM_104135.2.
UniGenei At.52162.

3D structure databases

ProteinModelPortali Q9SSQ9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 26914. 1 interaction.
STRINGi 3702.AT1G52570.1-P.

Proteomic databases

PaxDbi Q9SSQ9.
PRIDEi Q9SSQ9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G52570.1 ; AT1G52570.1 ; AT1G52570 .
GeneIDi 841689.
KEGGi ath:AT1G52570.

Organism-specific databases

TAIRi AT1G52570.

Phylogenomic databases

eggNOGi COG1502.
HOGENOMi HOG000240112.
InParanoidi Q9SSQ9.
KOi K01115.
OMAi RYPIGIA.
PhylomeDBi Q9SSQ9.

Enzyme and pathway databases

BioCyci ARA:AT1G52570-MONOMER.

Gene expression databases

Genevestigatori Q9SSQ9.

Family and domain databases

Gene3Di 2.60.40.150. 1 hit.
InterProi IPR000008. C2_dom.
IPR001736. PLipase_D/transphosphatidylase.
IPR024632. PLipase_D_C.
IPR015679. PLipase_D_fam.
IPR011402. PLipase_D_pln.
[Graphical view ]
PANTHERi PTHR18896. PTHR18896. 1 hit.
Pfami PF00168. C2. 1 hit.
PF12357. PLD_C. 1 hit.
PF00614. PLDc. 2 hits.
[Graphical view ]
PIRSFi PIRSF036470. PLD_plant. 1 hit.
SMARTi SM00239. C2. 1 hit.
SM00155. PLDc. 2 hits.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 1 hit.
PROSITEi PS50035. PLD. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Subcellular distribution and tissue expression of phospholipase Dalpha, Dbeta, and Dgamma in Arabidopsis."
    Fan L., Zheng S., Cui D., Wang X.
    Plant Physiol. 119:1371-1378(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiPLDA2_ARATH
AccessioniPrimary (citable) accession number: Q9SSQ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 1, 2000
Last modified: June 11, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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