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Protein

Phospholipase D alpha 2

Gene

PLDALPHA2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond. Plays an important role in various cellular processes, including phytohormone action and response to stress, characterized by acidification of the cell.

Catalytic activityi

A phosphatidylcholine + H2O = choline + a phosphatidate.

Cofactori

Ca2+Note: Ca2+ requirement for activity depends on pH. Active either under acidic conditions with micromolar levels of calcium (PIP2-dependent) or at neutral pH with millimolar levels of calcium (PIP2-independent).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei332 – 3321PROSITE-ProRule annotation
Active sitei334 – 3341PROSITE-ProRule annotation
Active sitei339 – 3391PROSITE-ProRule annotation
Active sitei661 – 6611PROSITE-ProRule annotation
Active sitei663 – 6631PROSITE-ProRule annotation
Active sitei668 – 6681PROSITE-ProRule annotation

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. N-acylphosphatidylethanolamine-specific phospholipase D activity Source: UniProtKB-EC
  3. phospholipase D activity Source: UniProtKB-EC

GO - Biological processi

  1. abscisic acid-activated signaling pathway Source: UniProtKB-KW
  2. ethylene-activated signaling pathway Source: UniProtKB-KW
  3. lipid catabolic process Source: UniProtKB-KW
  4. phosphatidylcholine metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Abscisic acid signaling pathway, Ethylene signaling pathway, Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium

Enzyme and pathway databases

BioCyciARA:AT1G52570-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase D alpha 2 (EC:3.1.4.4)
Short name:
AtPLDalpha2
Short name:
PLD alpha 2
Alternative name(s):
Choline phosphatase 2
Phosphatidylcholine-hydrolyzing phospholipase D 2
Gene namesi
Name:PLDALPHA2
Synonyms:PLD2
Ordered Locus Names:At1g52570
ORF Names:F6D8.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G52570.

Subcellular locationi

Cytoplasm 1 Publication. Membrane 1 Publication; Peripheral membrane protein 1 Publication. Vacuole 1 Publication. Cytoplasmic vesicleclathrin-coated vesicle 1 Publication
Note: Found in vacuoles and also associated with plasma, microsomal and mitochondrial membranes and in clathrin-coated vesicles. Not found in chloroplast or nuclei. Activation increases association of preexisting enzyme with membranes. The distribution of this conventional PLD between membrane-associated and soluble fractions varied from organ to organ and is calcium-regulated.

GO - Cellular componenti

  1. chloroplast envelope Source: TAIR
  2. clathrin-coated vesicle Source: UniProtKB-SubCell
  3. membrane Source: UniProtKB-SubCell
  4. vacuole Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 810810Phospholipase D alpha 2PRO_0000218809Add
BLAST

Proteomic databases

PaxDbiQ9SSQ9.
PRIDEiQ9SSQ9.

Expressioni

Tissue specificityi

Highly expressed in roots, stems and flowers, moderately in leaves, seedlings and siliques. Not detected in dry seeds.

Inductioni

Activated by abscisic acid (ABA), ethylene, heavy metal, cold, salt and osmotic stresses.

Gene expression databases

GenevestigatoriQ9SSQ9.

Interactioni

Protein-protein interaction databases

BioGridi26914. 1 interaction.
STRINGi3702.AT1G52570.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9SSQ9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 110110C2Add
BLAST
Domaini327 – 36539PLD phosphodiesterase 1PROSITE-ProRule annotationAdd
BLAST
Domaini656 – 68328PLD phosphodiesterase 2PROSITE-ProRule annotationAdd
BLAST

Domaini

C2 domain is a calcium-binding fold, and the binding promotes the protein association with membranes. A lower affinity toward calcium can be anticipated for PLD alpha due to the absence of two potential calcium ligands.

Sequence similaritiesi

Contains 1 C2 domain.Curated
Contains 2 PLD phosphodiesterase domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1502.
HOGENOMiHOG000240112.
InParanoidiQ9SSQ9.
KOiK01115.
OMAiDHEMPNQ.
PhylomeDBiQ9SSQ9.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR000008. C2_dom.
IPR001736. PLipase_D/transphosphatidylase.
IPR024632. PLipase_D_C.
IPR015679. PLipase_D_fam.
IPR011402. PLipase_D_pln.
[Graphical view]
PANTHERiPTHR18896. PTHR18896. 1 hit.
PfamiPF00168. C2. 1 hit.
PF12357. PLD_C. 1 hit.
PF00614. PLDc. 2 hits.
[Graphical view]
PIRSFiPIRSF036470. PLD_plant. 1 hit.
SMARTiSM00239. C2. 1 hit.
SM00155. PLDc. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
PROSITEiPS50035. PLD. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SSQ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEECLLHGRL HATIYEVDHL HAEGGRSGFL GSILANVEET IGVGKGETQL
60 70 80 90 100
YATIDLEKAR VGRTRKITKE PKNPKWFESF HIYCGHMAKH VIFTVKDANP
110 120 130 140 150
IGATLIGRGY IPVEDILHGE EVDRWVDILD NEKNPIAGGS KIHVKLQYFG
160 170 180 190 200
VEKDKNWNRG IKSAKFPGVP YTFFSQRRGC KVSLYQDAHI PGNFVPKIPL
210 220 230 240 250
AGGKNYEPHR CWEDIFDAIT NAKHLIYITG WSVYTEISLV RDSRRPKQGG
260 270 280 290 300
DVTVGELLKK KASEGVKVIL LVWDDRTSVD LLKKDGLMAT HDEETENFFR
310 320 330 340 350
GTDVNCILCP RNPDDGGSIV QNLQISTMFT HHQKIVVVDS EMPSGGSRSR
360 370 380 390 400
RIVSFVGGLD LCDGRYDTPF HSLFRTLDTA HHDDFHQPNF TGAAITKGGP
410 420 430 440 450
REPWHDIHCR LEGPIAWDVL YNFEQRWSRQ GGKDILVKMR ELGDIIIPPS
460 470 480 490 500
PVLFSEDHDV WNVQLFRSID GGAAAGFPDS PEAAAEAGLV SGKDNIIDRS
510 520 530 540 550
IQDAYIHAIR RAKDFIYIEN QYFLGSSFAW SADGIKPEEI NALHLIPKEL
560 570 580 590 600
SLKIVSKIKA GEKFKVYVVV PMWPEGIPES GSVQAILDWQ KRTMEMMYKD
610 620 630 640 650
VIKALRENGL EGEDPRDYLT FFCLGNREVK KDGEYEPSEK PEPDTDYIRA
660 670 680 690 700
QEARRFMIYV HTKMMIVDDE YIIIGSANIN QRSMDGARDS EIAMGGYQPY
710 720 730 740 750
HLSTRQPARG QIHGFRMSLW YEHLGMLDET FLDPSSQECI QKVNRVADKY
760 770 780 790 800
WDLYSSESLE HDLPGHLLRY PIGIASEGNI TELPGCEFFP DTKARILGVK
810
SDYMPPILTT
Length:810
Mass (Da):91,598
Last modified:April 30, 2000 - v1
Checksum:iF1FA36D8C9CFBCCA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC008016 Genomic DNA. Translation: AAD55607.1.
CP002684 Genomic DNA. Translation: AEE32825.1.
PIRiD96566.
RefSeqiNP_175666.1. NM_104135.2.
UniGeneiAt.52162.

Genome annotation databases

EnsemblPlantsiAT1G52570.1; AT1G52570.1; AT1G52570.
GeneIDi841689.
KEGGiath:AT1G52570.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC008016 Genomic DNA. Translation: AAD55607.1.
CP002684 Genomic DNA. Translation: AEE32825.1.
PIRiD96566.
RefSeqiNP_175666.1. NM_104135.2.
UniGeneiAt.52162.

3D structure databases

ProteinModelPortaliQ9SSQ9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi26914. 1 interaction.
STRINGi3702.AT1G52570.1-P.

Proteomic databases

PaxDbiQ9SSQ9.
PRIDEiQ9SSQ9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G52570.1; AT1G52570.1; AT1G52570.
GeneIDi841689.
KEGGiath:AT1G52570.

Organism-specific databases

TAIRiAT1G52570.

Phylogenomic databases

eggNOGiCOG1502.
HOGENOMiHOG000240112.
InParanoidiQ9SSQ9.
KOiK01115.
OMAiDHEMPNQ.
PhylomeDBiQ9SSQ9.

Enzyme and pathway databases

BioCyciARA:AT1G52570-MONOMER.

Gene expression databases

GenevestigatoriQ9SSQ9.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR000008. C2_dom.
IPR001736. PLipase_D/transphosphatidylase.
IPR024632. PLipase_D_C.
IPR015679. PLipase_D_fam.
IPR011402. PLipase_D_pln.
[Graphical view]
PANTHERiPTHR18896. PTHR18896. 1 hit.
PfamiPF00168. C2. 1 hit.
PF12357. PLD_C. 1 hit.
PF00614. PLDc. 2 hits.
[Graphical view]
PIRSFiPIRSF036470. PLD_plant. 1 hit.
SMARTiSM00239. C2. 1 hit.
SM00155. PLDc. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
PROSITEiPS50035. PLD. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Subcellular distribution and tissue expression of phospholipase Dalpha, Dbeta, and Dgamma in Arabidopsis."
    Fan L., Zheng S., Cui D., Wang X.
    Plant Physiol. 119:1371-1378(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiPLDA2_ARATH
AccessioniPrimary (citable) accession number: Q9SSQ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2001
Last sequence update: April 30, 2000
Last modified: March 31, 2015
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.