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Protein

Threonine synthase 2, chloroplastic

Gene

TS2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the gamma-elimination of phosphate from L-phosphohomoserine and the beta-addition of water to produce L-threonine.1 Publication

Miscellaneous

Binds 4 S-adenosyl-L-methionine (SAM) molecules per dimer. Although SAM3 and SAM4 have equivalent positions, their interactions with the protein are not identical. SAM3 interacts with Lys-172 and Asn-178 of monomer B, whereas SAM4 interacts only with Lys-172 of monomer A (By similarity).By similarity
Much less active than TS1 at physiological concentrations of S-adenosyl-methionine (20 µM).

Catalytic activityi

O-phospho-L-homoserine + H2O = L-threonine + phosphate.

Cofactori

Enzyme regulationi

Allosterically activated by S-adenosyl-methionine (SAM).

Pathwayi: L-threonine biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes L-threonine from L-aspartate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. AK1 (AXX17_At5g12690), Aspartokinase 2, chloroplastic (AK2), Aspartokinase (AXX17_At5g13510), Aspartokinase 1, chloroplastic (AK1), Aspartokinase (CARAB-AK-LYS), Aspartokinase 3, chloroplastic (AK3), Aspartokinase (AXX17_At3g01160), Bifunctional aspartokinase/homoserine dehydrogenase 2, chloroplastic (AKHSDH2), Aspartokinase (AXX17_At3g01160), Aspartokinase (AXX17_At3g01160), Bifunctional aspartokinase/homoserine dehydrogenase 1, chloroplastic (AKHSDH1)
  2. no protein annotated in this organism
  3. Homoserine dehydrogenase (AXX17_At5g21030), Homoserine dehydrogenase (At5g21060), Homoserine dehydrogenase (AXX17_At5g21030), Homoserine dehydrogenase (At5g21060), Homoserine dehydrogenase, Homoserine dehydrogenase (At5g21060), Bifunctional aspartokinase/homoserine dehydrogenase 2, chloroplastic (AKHSDH2), Bifunctional aspartokinase/homoserine dehydrogenase 1, chloroplastic (AKHSDH1)
  4. Homoserine kinase (HSK)
  5. Threonine synthase 2, chloroplastic (TS2), Threonine synthase 1, chloroplastic (TS1)
This subpathway is part of the pathway L-threonine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-threonine from L-aspartate, the pathway L-threonine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei163S-adenosyl-L-methionine 2; shared with dimeric partnerBy similarity1
Binding sitei164S-adenosyl-L-methionine 2; via amide nitrogen and carbonyl oxygen; shared with dimeric partnerBy similarity1
Binding sitei172S-adenosyl-L-methionine 3; in monomer BBy similarity1
Binding sitei172S-adenosyl-L-methionine 4; in monomer ABy similarity1
Binding sitei178S-adenosyl-L-methionine 3; in monomer BBy similarity1
Binding sitei464Pyridoxal phosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Lyase
Biological processAmino-acid biosynthesis, Threonine biosynthesis
LigandPyridoxal phosphate, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciARA:AT1G72810-MONOMER
UniPathwayiUPA00050; UER00065

Names & Taxonomyi

Protein namesi
Recommended name:
Threonine synthase 2, chloroplastic (EC:4.2.3.1)
Gene namesi
Name:TS2
Ordered Locus Names:At1g72810
ORF Names:F3N23.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G72810
TAIRilocus:2032632 AT1G72810

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 33ChloroplastCuratedAdd BLAST33
ChainiPRO_000037951634 – 516Threonine synthase 2, chloroplasticAdd BLAST483

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei194N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDbiQ9SSP5
PRIDEiQ9SSP5

Expressioni

Gene expression databases

ExpressionAtlasiQ9SSP5 baseline and differential
GenevisibleiQ9SSP5 AT

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT1G72810.1

Structurei

3D structure databases

ProteinModelPortaliQ9SSP5
SMRiQ9SSP5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni133 – 135S-adenosyl-L-methionine 1 binding; shared with dimeric partnerBy similarity3
Regioni156 – 158S-adenosyl-L-methionine 1 binding; shared with dimeric partnerBy similarity3
Regioni326 – 330Pyridoxal phosphate bindingBy similarity5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi150 – 153Poly-Asp4

Sequence similaritiesi

Belongs to the threonine synthase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IITN Eukaryota
COG0498 LUCA
HOGENOMiHOG000076502
InParanoidiQ9SSP5
KOiK01733
OMAiGTHLRAI
OrthoDBiEOG0936061W
PhylomeDBiQ9SSP5

Family and domain databases

InterProiView protein in InterPro
IPR001926 PLP-dep
IPR000634 Ser/Thr_deHydtase_PyrdxlP-BS
IPR004450 Thr_synthase-like
IPR036052 Trypto_synt_PLP_dependent
PfamiView protein in Pfam
PF00291 PALP, 1 hit
SUPFAMiSSF53686 SSF53686, 1 hit
TIGRFAMsiTIGR00260 thrC, 1 hit
PROSITEiView protein in PROSITE
PS00165 DEHYDRATASE_SER_THR, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SSP5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASFSLPHSA TYFPSHSETS LKPHSAASFT VRCTSASPAV PPQTPQKPRR
60 70 80 90 100
SPDENIRDEA RRRPHQLQNL SARYVPFNAP PSSTESYSLD EIVYRSQSGA
110 120 130 140 150
LLDVQHDFAA LKRYDGEFWR NLFDSRVGKT NWPYGSGVWS KKEWVLPEID
160 170 180 190 200
DDDIVSAFEG NSNLFWAERF GKQYLQMNDL WVKHCGISHT GSFKDLGMSV
210 220 230 240 250
LVSQVNRLRK MNKPVIGVGC ASTGDTSAAL SAYCASAGIP SIVFLPADKI
260 270 280 290 300
SMAQLVQPIA NGAFVLSIDT DFDGCMHLIR EVTAELPIYL ANSLNSLRLE
310 320 330 340 350
GQKTAAIEIL QQFNWQVPDW VIVPGGNLGN IYAFYKGFHM CKELGLVDRI
360 370 380 390 400
PRLVCAQAAN ANPLYLHYKS GFKEDFNPLK ANTTFASAIQ IGDPVSIDRA
410 420 430 440 450
VYALKKSNGI VEEATEEELM DATALADSTG MFICPHTGVA LTALMKLRKS
460 470 480 490 500
GVIGANDRTV VVSTAHGLKF TQSKIDYHSK NIKEMACRLA NPPVKVKAKF
510
GSVMDVLKEY LKSNDK
Length:516
Mass (Da):56,925
Last modified:May 1, 2000 - v1
Checksum:i6BB95F6CD125B1C9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC008017 Genomic DNA Translation: AAD55628.1
CP002684 Genomic DNA Translation: AEE35375.1
AY099629 mRNA Translation: AAM20480.1
BT002151 mRNA Translation: AAN72162.1
PIRiA96753
RefSeqiNP_565047.1, NM_105939.4
UniGeneiAt.11666

Genome annotation databases

EnsemblPlantsiAT1G72810.1; AT1G72810.1; AT1G72810
GeneIDi843612
GrameneiAT1G72810.1; AT1G72810.1; AT1G72810
KEGGiath:AT1G72810

Similar proteinsi

Entry informationi

Entry nameiTHRC2_ARATH
AccessioniPrimary (citable) accession number: Q9SSP5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: May 1, 2000
Last modified: April 25, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health