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Protein

(R)-mandelonitrile lyase-like

Gene

At1g73050

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

(R)-mandelonitrile = cyanide + benzaldehyde.

Cofactori

FADBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei492Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi55 – 82FADBy similarityAdd BLAST28

GO - Molecular functioni

Keywordsi

Molecular functionLyase
LigandFAD, Flavoprotein

Enzyme and pathway databases

BioCyciARA:AT1G73050-MONOMER.
ReactomeiR-ATH-6798163. Choline catabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
(R)-mandelonitrile lyase-like (EC:4.1.2.10)
Alternative name(s):
Hydroxynitrile lyase-like
Short name:
(R)-oxynitrilase-like
Gene namesi
Ordered Locus Names:At1g73050
ORF Names:F3N23.25
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G73050.
TAIRilocus:2032642. AT1G73050.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Sequence analysisAdd BLAST28
ChainiPRO_000041256229 – 552(R)-mandelonitrile lyase-likeAdd BLAST524

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi44N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi162N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi259N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi434N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Glycosylated.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9SSM2.
PRIDEiQ9SSM2.

Expressioni

Gene expression databases

GenevisibleiQ9SSM2. AT.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi3702.AT1G73050.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SSM2.
SMRiQ9SSM2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GMC oxidoreductase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1238. Eukaryota.
COG2303. LUCA.
HOGENOMiHOG000239349.
InParanoidiQ9SSM2.
KOiK08248.
OMAiIRQYTMT.
OrthoDBiEOG093609XW.
PhylomeDBiQ9SSM2.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
InterProiView protein in InterPro
IPR023753. FAD/NAD-binding_dom.
IPR012132. GMC_OxRdtase.
IPR000172. GMC_OxRdtase_N.
IPR007867. GMC_OxRtase_C.
PfamiView protein in Pfam
PF05199. GMC_oxred_C. 1 hit.
PF00732. GMC_oxred_N. 1 hit.
PIRSFiPIRSF000137. Alcohol_oxidase. 2 hits.
SUPFAMiSSF51905. SSF51905. 1 hit.
PROSITEiView protein in PROSITE
PS00623. GMC_OXRED_1. 1 hit.
PS00624. GMC_OXRED_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SSM2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKRIDSSLL YTALVVLLLL GVVHRSNARP RVNRPPGFMR FISNATDFAS
60 70 80 90 100
EDYYDYIIVG GGTAGCPLAA TLSQSFRVLL LERGGVPYNR PNVMSHDGFL
110 120 130 140 150
TTLTDVNNFD SPAQSFISEE GVPNARGRVL GGSSAINAGF YSRADKQFFE
160 170 180 190 200
NSGLVWDLSS VNQSYEWVER AIVFRPQLRT WQTAIRDALL EVGVHPFNGF
210 220 230 240 250
TLEHKVGTKI GGSTFDRTGR RHSSADLLRY ARSSNIRVAV YATVERVLLA
260 270 280 290 300
SSPSVSGSNV SAIGVVYRDQ LGRFHHALIR DRGEVILSAG ALGSPQLLFL
310 320 330 340 350
SGIGPRSYLS TWGIPVALDQ PHVGDFVYDN PRNGISIVPP VPMENSLIQV
360 370 380 390 400
VGVTEDGAFL EAASNVIPFA SPLHSVFIRA PASPLYVPVT TIMEKILGPV
410 420 430 440 450
SIGLLRLAST DVRINPVVRF NYFSDPQDLE RCVNGTRKIG EILRSRAMQD
460 470 480 490 500
FMIREWFGNR RFRFVGAPLP VDQSNDLVMA DFCRRTVSTI WHYHGGAVVG
510 520 530 540 550
KVVDSDLKVI GVNSLRLVDG STFNISPGTN PQATLMMLGR YMGLKMLRER

MR
Length:552
Mass (Da):60,783
Last modified:May 1, 2000 - v1
Checksum:i7747A5D5C2DA7C7A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti257G → R in ABE65766 (PubMed:17147637).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC008017 Genomic DNA. Translation: AAD55652.1.
CP002684 Genomic DNA. Translation: AEE35408.1.
DQ446423 mRNA. Translation: ABE65766.1.
PIRiA96756.
RefSeqiNP_177448.1. NM_105963.1.
UniGeneiAt.52489.

Genome annotation databases

EnsemblPlantsiAT1G73050.1; AT1G73050.1; AT1G73050.
GeneIDi843636.
GrameneiAT1G73050.1; AT1G73050.1; AT1G73050.
KEGGiath:AT1G73050.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiMDLL_ARATH
AccessioniPrimary (citable) accession number: Q9SSM2
Secondary accession number(s): Q1PFE0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: May 1, 2000
Last modified: July 5, 2017
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families