Reviewed, UniProtKB/Swiss-Prot Q9SSK1 (SYNC3_ARATH)

Names and origin

Protein names

Recommended name:
    Asparaginyl-tRNA synthetase, cytoplasmic 3
    EC=6.1.1.22
Alternative name(s):
    Asparagine--tRNA ligase 3
      Short name=AsnRS 3

Gene names

Name:	SYNC3
Ordered Locus Names:	At1g70980
ORF Names:	F15H11.17

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	571 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn).
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Contains 1 WHEP-TRS domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW asparagine-tRNA ligase activity Inferred from electronic annotation. Source: EC aspartate-tRNA ligase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 571	571	Asparaginyl-tRNA synthetase, cytoplasmic 3		PRO_0000176493
Regions
	Domain	233 – 289	57	WHEP-TRS

Sequences

	Sequence		Length	Mass (Da)
	Q9SSK1-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 1D5BF624B55FA1AE	FASTA	571	63,700
	10 20 30 40 50 60 MGDEIVPPAN QLAADNLEND GSTVQKAQFS DRVLIRSILG GGAKLAGQKV RIGGWVKTGR 70 80 90 100 110 120 QQGKGTFAFL EVNDGSCPAN LQVMVDSSLY DLSRLVATGT CVTVDGVLKI PPEGKGLKQS 130 140 150 160 170 180 IELSVETVIA VGTVDPTTYP LPKTKLTPEF LRDVLHLRSR TNLISAVARI RNALAFATHS 190 200 210 220 230 240 FFQEHSFLYI HTPIITTSDC EGAGEMFQVT TLINHTERVE QDLIDNPPPT EADVEAARLI 250 260 270 280 290 300 VKERGEAVAQ LKVAKASKEE ITASVAQLSV AKASLAHVEE RLRLKPGLPK NDGKIDYSND 310 320 330 340 350 360 FFGRQAFLTV SGQLQVETYA CALSSVYTFG PTFRAENSHT SRHLAEFWMV EPEIAFADIH 370 380 390 400 410 420 DDMNCAEAYV KYMCKWLMDK CGDDMELMDK NVDEGCTKRL NMVAKASFKR VTYTEAIERL 430 440 450 460 470 480 EKAVAQGKVV FDNKVEWGID LASEHERYLT EVEFDQKPII VYNYPKGIKA FYMRLNDDEK 490 500 510 520 530 540 TVAAMDVLVP KVGELIGGSQ REERYDVIKQ RIEEMGLPME PYEWYLDLRR YGTVKHCGFG 550 560 570 LGFERMIQFA TGIDNIRDVI PFPRYPGKAD L

References

[1]

"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC008148 Genomic DNA. Translation: AAD55509.1.
IPI	IPI00543324.
PIR	B96734.
RefSeq	NP_177254.1.
UniGene	At.35245
3D structure databases
HSSP	HSSP built from PDB template 1B8A based on UniProtKB Q52428.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9SSK1.
Proteomic databases
PRIDE	Q9SSK1.
Genome annotation databases
GeneID	843436.
GenomeReviews	Gene locus AT1G70980 in contig CT485782_GR.
KEGG	ath:AT1G70980.
NMPDR	fig|3702.1.peg.6584.
Organism-specific databases
GeneFarm	2805. 269.
TAIR	At1g70980.
Phylogenomic databases
OMA	LINHTER.
Enzyme and pathway databases
BRENDA	6.1.1.22. 302.
Gene expression databases
ArrayExpress	Q9SSK1.
Genevestigator	Q9SSK1.
GermOnline	AT1G70980. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR004365. NA_bd_OB_tRNA-helicase. IPR000738. WHEP-TRS. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00152. tRNA-synt_2. 2 hits. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00457. asnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. PS00762. WHEP_TRS_1. False negative. PS51185. WHEP_TRS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYNC3_ARATH

Accession

Primary (citable) accession number: Q9SSK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 31, 2002
Last sequence update:	May 1, 2000
Last modified:	November 3, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)