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Protein

Ethylene-responsive transcription factor RAP2-12

Gene

RAP2-12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor involved in the activation of hypoxic gene expression and in ethylene response. Partially redundant with RAP2-2. Acts as a downstream regulator in the ethylene signaling pathway.2 Publications

Enzyme regulationi

Ethylene-responsive transcription factor RAP2-12, N-terminally processed: The N-terminal cysteine residue of can be oxidized by PCO1 or PCO2, thus preparing the protein for N-end rule pathway-mediated proteasomal degradation.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi124 – 18158AP2/ERFPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • detection of hypoxia Source: TAIR
  • ethylene-activated signaling pathway Source: UniProtKB-KW
  • response to hypoxia Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Ethylene signaling pathway, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ethylene-responsive transcription factor RAP2-12
Alternative name(s):
Protein RELATED TO APETALA2 12
Gene namesi
Name:RAP2-12
Synonyms:ERF074
Ordered Locus Names:At1g53910
ORF Names:T18A20.14
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G53910.

Subcellular locationi

  • Cell membrane 1 Publication
  • Nucleus PROSITE-ProRule annotation1 Publication

  • Note: Localizes to the plasma membrane under aerobic conditions, but accumulates in the nucleus upon hypoxia.

GO - Cellular componenti

  • nucleus Source: TAIR
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi2 – 21C → A: Increased lifetime of the protein and accumulation in the nucleus. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 358358Ethylene-responsive transcription factor RAP2-12PRO_0000297941Add
BLAST

Proteomic databases

PaxDbiQ9SSA8.

Expressioni

Tissue specificityi

Highly expressed in seedlings, leaves, flowers, siliques and germinating seeds.1 Publication

Inductioni

Up-regulated by hypoxia but not by ethylene.1 Publication

Gene expression databases

ExpressionAtlasiQ9SSA8. baseline and differential.
GenevisibleiQ9SSA8. AT.

Interactioni

Subunit structurei

Interacts with ACBP1 and ACBP2.1 Publication

Protein-protein interaction databases

BioGridi27054. 2 interactions.
DIPiDIP-60469N.
IntActiQ9SSA8. 1 interaction.
STRINGi3702.AT1G53910.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SSA8.
SMRiQ9SSA8. Positions 124-181.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminus (1-13) is important for the regulation of the oxygen-dependent activation of the transcription factor activity, an internal region (123-177) is required for the interactions with ACPB1 and ACPB2, while the C-terminus (343-358) is required for positive regulation of gene transcription.1 Publication

Sequence similaritiesi

Contains 1 AP2/ERF DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IWFM. Eukaryota.
ENOG410YERP. LUCA.
HOGENOMiHOG000237624.
InParanoidiQ9SSA8.
KOiK09286.
OMAiWSIDEIH.
OrthoDBiEOG09360HNM.
PhylomeDBiQ9SSA8.

Family and domain databases

Gene3Di3.30.730.10. 1 hit.
InterProiIPR001471. AP2/ERF_dom.
IPR016177. DNA-bd_dom.
[Graphical view]
PfamiPF00847. AP2. 1 hit.
[Graphical view]
PRINTSiPR00367. ETHRSPELEMNT.
SMARTiSM00380. AP2. 1 hit.
[Graphical view]
SUPFAMiSSF54171. SSF54171. 1 hit.
PROSITEiPS51032. AP2_ERF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9SSA8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MCGGAIISDF IPPPRSRRVT SEFIWPDLKK NLKGSKKSSK NRSNFFDFDA
60 70 80 90 100
EFEADFQGFK DDSSIDCDDD FDVGDVFADV KPFVFTSTPK PAVSAAAEGS
110 120 130 140 150
VFGKKVTGLD GDAEKSANRK RKNQYRGIRQ RPWGKWAAEI RDPREGARIW
160 170 180 190 200
LGTFKTAEEA ARAYDAAARR IRGSKAKVNF PEENMKANSQ KRSVKANLQK
210 220 230 240 250
PVAKPNPNPS PALVQNSNIS FENMCFMEEK HQVSNNNNNQ FGMTNSVDAG
260 270 280 290 300
CNGYQYFSSD QGSNSFDCSE FGWSDQAPIT PDISSAVINN NNSALFFEEA
310 320 330 340 350
NPAKKLKSMD FETPYNNTEW DASLDFLNED AVTTQDNGAN PMDLWSIDEI

HSMIGGVF
Length:358
Mass (Da):39,801
Last modified:May 1, 2000 - v1
Checksum:iCF8232CBE3C9FE35
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti112 – 1121D → E in AAM65746 (Ref. 4) Curated
Sequence conflicti185 – 1851M → L in AAM65746 (Ref. 4) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC009324 Genomic DNA. Translation: AAF02863.1.
CP002684 Genomic DNA. Translation: AEE33022.1.
CP002684 Genomic DNA. Translation: AEE33023.1.
AY037260 mRNA. Translation: AAK59861.1.
AY057545 mRNA. Translation: AAL09785.1.
AY113051 mRNA. Translation: AAM47359.1.
AY088204 mRNA. Translation: AAM65746.1.
AF003105 mRNA. Translation: AAC49778.1.
PIRiD96579.
RefSeqiNP_001031185.1. NM_001036108.2. [Q9SSA8-1]
NP_175794.1. NM_104269.3. [Q9SSA8-1]
UniGeneiAt.20952.
At.21295.

Genome annotation databases

EnsemblPlantsiAT1G53910.1; AT1G53910.1; AT1G53910. [Q9SSA8-1]
AT1G53910.2; AT1G53910.2; AT1G53910. [Q9SSA8-1]
GeneIDi841829.
KEGGiath:AT1G53910.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC009324 Genomic DNA. Translation: AAF02863.1.
CP002684 Genomic DNA. Translation: AEE33022.1.
CP002684 Genomic DNA. Translation: AEE33023.1.
AY037260 mRNA. Translation: AAK59861.1.
AY057545 mRNA. Translation: AAL09785.1.
AY113051 mRNA. Translation: AAM47359.1.
AY088204 mRNA. Translation: AAM65746.1.
AF003105 mRNA. Translation: AAC49778.1.
PIRiD96579.
RefSeqiNP_001031185.1. NM_001036108.2. [Q9SSA8-1]
NP_175794.1. NM_104269.3. [Q9SSA8-1]
UniGeneiAt.20952.
At.21295.

3D structure databases

ProteinModelPortaliQ9SSA8.
SMRiQ9SSA8. Positions 124-181.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi27054. 2 interactions.
DIPiDIP-60469N.
IntActiQ9SSA8. 1 interaction.
STRINGi3702.AT1G53910.1.

Proteomic databases

PaxDbiQ9SSA8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G53910.1; AT1G53910.1; AT1G53910. [Q9SSA8-1]
AT1G53910.2; AT1G53910.2; AT1G53910. [Q9SSA8-1]
GeneIDi841829.
KEGGiath:AT1G53910.

Organism-specific databases

TAIRiAT1G53910.

Phylogenomic databases

eggNOGiENOG410IWFM. Eukaryota.
ENOG410YERP. LUCA.
HOGENOMiHOG000237624.
InParanoidiQ9SSA8.
KOiK09286.
OMAiWSIDEIH.
OrthoDBiEOG09360HNM.
PhylomeDBiQ9SSA8.

Miscellaneous databases

PROiQ9SSA8.

Gene expression databases

ExpressionAtlasiQ9SSA8. baseline and differential.
GenevisibleiQ9SSA8. AT.

Family and domain databases

Gene3Di3.30.730.10. 1 hit.
InterProiIPR001471. AP2/ERF_dom.
IPR016177. DNA-bd_dom.
[Graphical view]
PfamiPF00847. AP2. 1 hit.
[Graphical view]
PRINTSiPR00367. ETHRSPELEMNT.
SMARTiSM00380. AP2. 1 hit.
[Graphical view]
SUPFAMiSSF54171. SSF54171. 1 hit.
PROSITEiPS51032. AP2_ERF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRA212_ARATH
AccessioniPrimary (citable) accession number: Q9SSA8
Secondary accession number(s): O23113, Q8L9V3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 21, 2007
Last sequence update: May 1, 2000
Last modified: September 7, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The N-terminus qualifies RAP2-12 as candidate substrate of the N-end rule pathway for protein destabilization. The oxygen-dependent oxidation of Cys-2 prevents hypoxic gene expression via the destabilization of RAP2-12 in air. When the oxygen concentration decreases, Cys oxidation is prevented and an active RAP2.12 accumulates in the nucleus.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.