Q9SS90 (RGLG1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
E3 ubiquitin-protein ligase RGLG1
EC=6.3.2.-

Alternative name(s):
RING domain ligase 1

Gene names

Name:	RGLG1
Ordered Locus Names:	At3g01650
ORF Names:	F4P13.19

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	489 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	E3 ubiquitin-protein ligase that mediates the formation of 'Lys-63'-linked multiubiquitin chains. Regulates apical dominance by acting on the auxin transport proteins abundance. Ref.4
Subunit structure	Interacts with the heterodimer UBC35/UEV1B. Ref.4
Subcellular location	Cell membrane; Lipid-anchor.
Tissue specificity	Ubiquitously expressed. Ref.4
Post-translational modification	N-myristoylated Probable.
Disruption phenotype	Plants do not show any phenotype alteration due to the redundancy with RGLG2. Double mutant RGLG1-RGLG2 shows a complete loss of apical dominance. Ref.4
Sequence similarities	Contains 1 RING-type zinc finger. Contains 1 VWFA domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cell membrane Membrane
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Ligase
PTM	Lipoprotein Myristate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	auxin metabolic process Inferred from genetic interaction Ref.4. Source: TAIR cytokinin metabolic process Inferred from genetic interaction Ref.4. Source: TAIR
Cellular component	plasma membrane Inferred from direct assay Ref.4. Source: TAIR
Molecular function	ligase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Potential
	Chain	2 – 489	488	E3 ubiquitin-protein ligase RGLG1		PRO_0000344783
Regions
	Domain	154 – 357	204	VWFA
	Zinc finger	446 – 479	34	RING-type
	Compositional bias	10 – 17	8	Poly-Ser
	Compositional bias	25 – 115	91	Pro-rich
Amino acid modifications
	Lipidation	2	1	N-myristoyl glycine Potential
Experimental info
	Sequence conflict	404	1	P → L in BX824117. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9SS90 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: C677530E1B20B518	FASTA	489	53,312
	10 20 30 40 50 60 MGGGNSKEES SSPSSSSWAS HQSYPQYGPD SYNYPPPPTY APAPSPAPAP APVPAPSPAS 70 80 90 100 110 120 SYGPQYSQEG YASQPNNPPP PTYAPAPSPA SSYGHQYSQE GYASAASQPN YPPPPSQSQV 130 140 150 160 170 180 ADRKKFDRRY SKISDNYSSL LQVSEALGRA GLESSNLIVG IDFTKSNEWT GAKSFNRKSL 190 200 210 220 230 240 HHLSNTPNPY EQAITIIGRT LAAFDEDNLI PCYGFGDAST HDQDVFSFYP EGRFCNGFEE 250 260 270 280 290 300 VLARYREIVP QLKLAGPTSF APIIEMAMTV VEQSSGQYHV LVIIADGQVT RSVDTEHGRL 310 320 330 340 350 360 SPQEQKTVDA IVKASTLPLS IVLVGVGDGP WDMMQEFDDN IPARAFDNFQ FVNFTEIMSK 370 380 390 400 410 420 NKDQSRKETE FALSALMEIP PQYKATIELN LLGVRNGNIP QRIPLPPPVQ SGSSFSSSRI 430 440 450 460 470 480 PNFEPSVPPY PFESKQMSSA DDIQLCPICL SNPKNMAFGC GHQTCCECGP DLKVCPICRA PIQTRIKLY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation." Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M. Genome Res. 14:406-413(2004) [PubMed: 14993207] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Ubiquitin lysine 63 chain forming ligases regulate apical dominance in Arabidopsis." Yin X.-J., Volk S., Ljung K., Mehlmer N., Dolezal K., Ditengou F., Hanano S., Davis S.J., Schmelzer E., Sandberg G., Teige M., Palme K., Pickart C., Bachmair A. Plant Cell 19:1898-1911(2007) [PubMed: 17586653] [Abstract] Cited for: IDENTIFICATION, FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH UBC35, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC009325 Genomic DNA. Translation: AAF01562.1. CP002686 Genomic DNA. Translation: AEE73699.1. BX824117 mRNA. No translation available.
IPI	IPI00531620.
RefSeq	NP_186814.1. NM_111031.3.
UniGene	At.22440.
3D structure databases
ProteinModelPortal	Q9SS90.
SMR	Q9SS90. Positions 443-489.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9SS90.
Proteomic databases
PRIDE	Q9SS90.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G01650.1; AT3G01650.1; AT3G01650.
GeneID	819911.
GenomeReviews	Gene locus AT3G01650 in contig BA000014_GR.
KEGG	ath:AT3G01650.
NMPDR	fig|3702.1.peg.12093.
Organism-specific databases
TAIR	At3g01650.
Phylogenomic databases
eggNOG	KOG1327.
GeneTree	EPGT00070000028159.
HOGENOM	HBG746179.
InParanoid	Q9SS90.
OMA	NRKSLHH.
PhylomeDB	Q9SS90.
ProtClustDB	CLSN2915600.
Gene expression databases
Genevestigator	Q9SS90.
Family and domain databases
InterPro	IPR010734. Copine. IPR002035. VWF_A. IPR001841. Znf_RING. IPR013083. Znf_RING/FYVE/PHD. [Graphical view]
Gene3D	G3DSA:3.30.40.10. Znf_RING/FYVE/PHD. 1 hit.
Pfam	PF07002. Copine. 1 hit. [Graphical view]
SMART	SM00184. RING. 1 hit. SM00327. VWA. 1 hit. [Graphical view]
PROSITE	PS50234. VWFA. False negative. PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RGLG1_ARATH

Accession

Primary (citable) accession number: Q9SS90

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program