Q9SS38 (GYRBP_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase subunit B, chloroplastic
EC=5.99.1.3

Gene names

Name:	GYRBC
Ordered Locus Names:	At3g10270
ORF Names:	F14P13.13

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	661 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner. Ref.2
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA.
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis By similarity.
Subcellular location	Plastid › chloroplast Ref.2.
Sequence similarities	Belongs to the type II topoisomerase family.
Sequence caution	The sequence AAF02815.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast Potential
	Chain	? – 661		DNA gyrase subunit B, chloroplastic		PRO_0000247948

Sequences

	Sequence		Length	Mass (Da)
	Q9SS38 [UniParc]. Last modified September 1, 2009. Version 2. Checksum: 1DD7537564D9668F	FASTA	661	72,899
	10 20 30 40 50 60 MSSTMESLQE SSTSKDYSSE HIQVLEGLDP VRKRPGMYIG STGSRGLHHL VYEILDNAID 70 80 90 100 110 120 EAQAGFASKI DVVLHSDDSV SISDNGRGIP TDLHPATGKS SLETVLTVLH AGGKFGGKSS 130 140 150 160 170 180 GYSVSGGLHG VGLSVVNALS EALEVIVRRD GMEFQQKYSR GKPVTTLTCH VLPPESRGTQ 190 200 210 220 230 240 GTCIRFWPDK EGFALFTTAI QFDHNTIAGR IRELAFLNPK VTISLKKEDD DPERDVYSEY 250 260 270 280 290 300 FYAGGLTEYV SWLNTDKKPL HDVLGFRKEI NGSTVDVSLQ WCSDAYSDTM LGYANSIRTI 310 320 330 340 350 360 DGGTHIEGVK ASLTRTLNSL AKKLKVIKEK DISLSGEHVR EGLTCIVSVK VPNPEFEGQT 370 380 390 400 410 420 KTRLGNPEVR KIVDQSVQEY LTEYLELHPD VLESIISKSL NAYKAALAAK RARELVRSKS 430 440 450 460 470 480 VLKSSSLPGK LADCSSTDPA ESEIFIVEGD SAGGSAKQGR DRRFQAILPL RGKILNIERK 490 500 510 520 530 540 DEAAMYKNEE IQNLILGLGL GVKGEDFNKE NLRYHKIIIL TDADVDGAHI RTLLLTFFFR 550 560 570 580 590 600 YQRALFDAGC IYVGVPPLFK VERGKQAHYC YDDAALKKIT ASFPGNASYN IQRFKGLGEM 610 620 630 640 650 660 MPAQLWETTM NPDTRILKQL VVDDAAETNM VFSSLMGARV DVRKELIKSA ATRMNLENLD I

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Arabidopsis thaliana DNA gyrase is targeted to chloroplasts and mitochondria." Wall M.K., Mitchenall L.A., Maxwell A. Proc. Natl. Acad. Sci. U.S.A. 101:7821-7826(2004) [PubMed: 15136745] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC009400 Genomic DNA. Translation: AAF02815.1. Different initiation.
IPI	IPI00547129.
3D structure databases
ProteinModelPortal	Q9SS38.
SMR	Q9SS38. Positions 21-416, 429-660.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9SS38.
Proteomic databases
PRIDE	Q9SS38.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GenomeReviews	Gene locus AT3G10270 in contig BA000014_GR.
NMPDR	fig|3702.1.peg.13053.
Organism-specific databases
GeneFarm	4079. 413.
TAIR	At3g10270.
Phylogenomic databases
eggNOG	KOG0355.
HOGENOM	HBG304889.
InParanoid	Q9SS38.
Gene expression databases
Genevestigator	Q9SS38.
GermOnline	AT3G10270. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR003594. ATPase-like_ATP-bd. IPR011557. GyrB. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR001241. Topo_IIA. IPR013759. Topo_IIA_B/N_ab. IPR002288. Topo_IIA_B_C. IPR013506. Topo_IIA_bsu_dom2. IPR013760. Topo_IIA_cen. IPR018522. TopoIIA_CS. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit. G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit. G3DSA:3.40.50.670. Topo_IIA_B/N_ab. 1 hit.
Pfam	PF00204. DNA_gyraseB. 1 hit. PF00986. DNA_gyraseB_C. 1 hit. PF02518. HATPase_c. 1 hit. [Graphical view]
PRINTS	PR00418. TPI2FAMILY.
SMART	SM00387. HATPase_c. 1 hit. SM00433. TOP2c. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01059. GyrB. 1 hit.
PROSITE	PS00177. TOPOISOMERASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GYRBP_ARATH

Accession

Primary (citable) accession number: Q9SS38

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	September 1, 2009
Last modified:	January 25, 2012
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program