Q9SS01 (AAE20_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Benzoate--CoA ligase, peroxisomal
EC=6.2.1.25

Alternative name(s):
Acyl-activating enzyme 20
Protein BENZOYLOXYGLUCOSINOLATE 1

Gene names

Name:	AAE20
Synonyms:	BZO1
Ordered Locus Names:	At1g65880
ORF Names:	F12P19.5

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	580 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Benzoate--CoA ligase involved in benzoyloxyglucosinolate biosynthesis in seeds. Glucosinolates are secondary metabolites involved in pathogen and insect defense of cruciferous plants. Ref.4
Catalytic activity	ATP + benzoate + CoA = AMP + diphosphate + benzoyl-CoA. Ref.4
Subcellular location	Peroxisome Potential.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Peroxisome
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	defense response Inferred from electronic annotation. Source: UniProtKB-KW glucosinolate biosynthetic process Inferred from mutant phenotype Ref.4. Source: TAIR
Cellular_component	peroxisome Inferred from sequence or structural similarity Ref.4. Source: TAIR
Molecular_function	benzoate-CoA ligase activity Inferred from direct assay Ref.4. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 580	580	Benzoate--CoA ligase, peroxisomal		PRO_0000415730
Regions
	Motif	578 – 580	3	Microbody targeting signal Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9SS01 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 3855327A59D6E3A7	FASTA	580	64,941
	10 20 30 40 50 60 MDDLALCEAN NVPLTPMTFL KRASECYPNR TSIIYGKTRF TWPQTYDRCC RLAASLISLN 70 80 90 100 110 120 ISKNDVVSVM APNTPALYEM HFAVPMAGAV LNPINTRLDA TSIAAILRHA KPKILFLDRS 130 140 150 160 170 180 FEALARESLH LLSSEDSNLN LPVIFIHEND FPKRASFEEL DYECLIQRGE PTPSMVARMF 190 200 210 220 230 240 RIQDEHDPIS LNYTSGTTAD PKGVVISHRG AYLCTLSAII GWEMGTCPVY LWTLPMFHCN 250 260 270 280 290 300 GWTFTWGTAA RGGTSVCMRH VTAPEIYKNI EMHNVTHMCC VPTVFNILLK GNSLDLSPRS 310 320 330 340 350 360 GPVHVLTGGS PPPAALVKKV QRLGFQVMHA YGQTEATGPI LFCEWQDEWN RLPENQQMEL 370 380 390 400 410 420 KARQGISILG LADVDVKNKE TQKSAPRDGK TMGEILIKGS SIMKGYLKNP KATFEAFKHG 430 440 450 460 470 480 WLNTGDVGVI HPDGHVEIKD RSKDIIISGG ENISSVEVEN VLYKYPKVLE TAVVAMPHPT 490 500 510 520 530 540 WGETPCAFVV LEKSETTIKE DRVDKFQTRE RNLIEYCREN LPHFMCPRKV VFLEELPKNG 550 560 570 580 NGKILKPKLR DIAKGLVVED EINVIAKEVK RPVGHFISRL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Arabidopsis contains a large superfamily of acyl-activating enzymes. Phylogenetic and biochemical analysis reveals a new class of acyl-coenzyme a synthetases." Shockey J.M., Fulda M.S., Browse J. Plant Physiol. 132:1065-1076(2003) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY.
[4]	"Characterization of seed-specific benzoyloxyglucosinolate mutations in Arabidopsis thaliana." Kliebenstein D.J., D'Auria J.C., Behere A.S., Kim J.H., Gunderson K.L., Breen J.N., Lee G., Gershenzon J., Last R.L., Jander G. Plant J. 51:1062-1076(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC009513 Genomic DNA. Translation: AAF06049.1. CP002684 Genomic DNA. Translation: AEE34436.1.
IPI	IPI00544157.
PIR	A96683.
RefSeq	NP_176763.1. NM_105260.2.
UniGene	At.35826.
3D structure databases
HSSP	HSSP built from PDB template 1LCI based on UniProtKB P08659.
ProteinModelPortal	Q9SS01.
SMR	Q9SS01. Positions 19-567.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT1G65880.1-P.
Proteomic databases
PRIDE	Q9SS01.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G65880.1; AT1G65880.1; AT1G65880.
GeneID	842900.
KEGG	ath:AT1G65880.
Organism-specific databases
TAIR	At1g65880.
Phylogenomic databases
HOGENOM	HOG000230005.
InParanoid	Q9SS01.
OMA	LRHGECK.
PhylomeDB	Q9SS01.
ProtClustDB	PLN03102.
Enzyme and pathway databases
BioCyc	MetaCyc:AT1G65880-MONOMER.
Gene expression databases
Genevestigator	Q9SS01.
Family and domain databases
InterPro	IPR000873. AMP-dep_Synth/Lig. IPR025110. DUF4009. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. PF13193. DUF4009. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AAE20_ARATH

Accession

Primary (citable) accession number: Q9SS01

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 22, 2012
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program