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Q9SRZ6

- ICDHC_ARATH

UniProt

Q9SRZ6 - ICDHC_ARATH

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Protein

Cytosolic isocitrate dehydrogenase [NADP]

Gene

CICDH

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

May supply 2-oxoglutarate for amino acid biosynthesis and ammonia assimilation via the glutamine synthetase/glutamate synthase (GS/GOGAT) pathway. May be involved in the production of NADPH to promote redox signaling or homeostasis in response to oxidative stress.1 Publication

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactori

Binds 1 magnesium or manganese ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei79 – 791SubstrateBy similarity
Binding sitei84 – 841NADPBy similarity
Binding sitei111 – 1111SubstrateBy similarity
Binding sitei134 – 1341SubstrateBy similarity
Sitei141 – 1411Critical for catalysisBy similarity
Sitei212 – 2121Critical for catalysisBy similarity
Metal bindingi252 – 2521Magnesium or manganeseBy similarity
Binding sitei260 – 2601NADPBy similarity
Metal bindingi275 – 2751Magnesium or manganeseBy similarity
Binding sitei328 – 3281NADP; via amide nitrogen and carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi77 – 793NADPBy similarity
Nucleotide bindingi310 – 3156NADPBy similarity

GO - Molecular functioni

  1. copper ion binding Source: TAIR
  2. isocitrate dehydrogenase (NADP+) activity Source: TAIR
  3. magnesium ion binding Source: InterPro
  4. NAD binding Source: InterPro

GO - Biological processi

  1. defense response to bacterium Source: TAIR
  2. isocitrate metabolic process Source: TAIR
  3. NADP metabolic process Source: TAIR
  4. response to cadmium ion Source: TAIR
  5. response to salt stress Source: TAIR
  6. response to zinc ion Source: TAIR
  7. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Plant defense, Stress response, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:AT1G65930-MONOMER.
ReactomeiREACT_214122. Abnormal conversion of 2-oxoglutarate to 2-hydroxyglutarate.
REACT_218202. NADPH regeneration.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic isocitrate dehydrogenase [NADP] (EC:1.1.1.42)
Gene namesi
Name:CICDH
Ordered Locus Names:At1g65930
ORF Names:F12P19.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G65930.

Subcellular locationi

GO - Cellular componenti

  1. apoplast Source: TAIR
  2. chloroplast stroma Source: TAIR
  3. cytosol Source: TAIR
  4. plasma membrane Source: TAIR
  5. plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Slight reduction in plant growth. Constitutive expression of pathogenesis-related genes and enhanced resistance to the bacterial pathogen P.syringae pv. tomato.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 410410Cytosolic isocitrate dehydrogenase [NADP]PRO_0000421963Add
BLAST

Proteomic databases

PRIDEiQ9SRZ6.

Expressioni

Gene expression databases

ExpressionAtlasiQ9SRZ6. baseline and differential.
GenevestigatoriQ9SRZ6.

Interactioni

Protein-protein interaction databases

IntActiQ9SRZ6. 1 interaction.
STRINGi3702.AT1G65930.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9SRZ6.
SMRiQ9SRZ6. Positions 5-404.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni96 – 1027Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

InParanoidiQ9SRZ6.
KOiK00031.
OMAiNEHYVIT.
PhylomeDBiQ9SRZ6.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SRZ6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAFEKIKVAN PIVEMDGDEM TRVIWKSIKD KLITPFVELD IKYFDLGLPH
60 70 80 90 100
RDATDDKVTI ESAEATKKYN VAIKCATITP DEGRVTEFGL KQMWRSPNGT
110 120 130 140 150
IRNILNGTVF REPIICKNVP KLVPGWTKPI CIGRHAFGDQ YRATDAVIKG
160 170 180 190 200
PGKLTMTFEG KDGKTETEVF TFTGEGGVAM AMYNTDESIR AFADASMNTA
210 220 230 240 250
YEKKWPLYLS TKNTILKKYD GRFKDIFQEV YEASWKSKYD AAGIWYEHRL
260 270 280 290 300
IDDMVAYALK SEGGYVWACK NYDGDVQSDF LAQGFGSLGL MTSVLVCPDG
310 320 330 340 350
KTIEAEAAHG TVTRHFRVHQ KGGETSTNSI ASIFAWTRGL AHRAKLDDNA
360 370 380 390 400
KLLDFTEKLE AACVGTVESG KMTKDLALII HGSKLSRDTY LNTEEFIDAV
410
AAELKERLNA
Length:410
Mass (Da):45,746
Last modified:May 1, 2000 - v1
Checksum:i6CC3CA11AB631D28
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti181 – 1811A → V in CAD24782. 1 PublicationCurated
Sequence conflicti262 – 2621E → K in AAM65674. 1 PublicationCurated
Sequence conflicti280 – 2801F → L in CAD24782. 1 PublicationCurated
Sequence conflicti302 – 3021T → S in CAD24782. 1 PublicationCurated
Sequence conflicti322 – 3221G → A in AAM65674. 1 PublicationCurated
Sequence conflicti395 – 3951E → D in AAM13090. (PubMed:14593172)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ437268 mRNA. Translation: CAD24782.1.
AC009513 Genomic DNA. Translation: AAF06054.1.
CP002684 Genomic DNA. Translation: AEE34442.1.
AF419575 mRNA. Translation: AAL31907.1.
AY045631 mRNA. Translation: AAK73989.1.
AY093091 mRNA. Translation: AAM13090.1.
AY097340 mRNA. Translation: AAM19856.1.
BT002400 mRNA. Translation: AAO00760.1.
AY088129 mRNA. Translation: AAM65674.1.
PIRiF96683.
RefSeqiNP_176768.1. NM_105265.4.
UniGeneiAt.24713.

Genome annotation databases

EnsemblPlantsiAT1G65930.1; AT1G65930.1; AT1G65930.
GeneIDi842905.
KEGGiath:AT1G65930.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ437268 mRNA. Translation: CAD24782.1 .
AC009513 Genomic DNA. Translation: AAF06054.1 .
CP002684 Genomic DNA. Translation: AEE34442.1 .
AF419575 mRNA. Translation: AAL31907.1 .
AY045631 mRNA. Translation: AAK73989.1 .
AY093091 mRNA. Translation: AAM13090.1 .
AY097340 mRNA. Translation: AAM19856.1 .
BT002400 mRNA. Translation: AAO00760.1 .
AY088129 mRNA. Translation: AAM65674.1 .
PIRi F96683.
RefSeqi NP_176768.1. NM_105265.4.
UniGenei At.24713.

3D structure databases

ProteinModelPortali Q9SRZ6.
SMRi Q9SRZ6. Positions 5-404.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9SRZ6. 1 interaction.
STRINGi 3702.AT1G65930.1-P.

Proteomic databases

PRIDEi Q9SRZ6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G65930.1 ; AT1G65930.1 ; AT1G65930 .
GeneIDi 842905.
KEGGi ath:AT1G65930.

Organism-specific databases

TAIRi AT1G65930.

Phylogenomic databases

InParanoidi Q9SRZ6.
KOi K00031.
OMAi NEHYVIT.
PhylomeDBi Q9SRZ6.

Enzyme and pathway databases

BioCyci MetaCyc:AT1G65930-MONOMER.
Reactomei REACT_214122. Abnormal conversion of 2-oxoglutarate to 2-hydroxyglutarate.
REACT_218202. NADPH regeneration.

Gene expression databases

ExpressionAtlasi Q9SRZ6. baseline and differential.
Genevestigatori Q9SRZ6.

Family and domain databases

Gene3Di 3.40.718.10. 1 hit.
InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view ]
PANTHERi PTHR11822. PTHR11822. 1 hit.
Pfami PF00180. Iso_dh. 1 hit.
[Graphical view ]
PIRSFi PIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsi TIGR00127. nadp_idh_euk. 1 hit.
PROSITEi PS00470. IDH_IMDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of a full lenght cDNA encoding for NADP+-isocitrate dehydrogenase from Arabidopsis thaliana."
    Pistelli L., De Bellis L., Alpi A., Gonzali S.
    Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "Cytosolic NADP-dependent isocitrate dehydrogenase contributes to redox homeostasis and the regulation of pathogen responses in Arabidopsis leaves."
    Mhamdi A., Mauve C., Gouia H., Saindrenan P., Hodges M., Noctor G.
    Plant Cell Environ. 33:1112-1123(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiICDHC_ARATH
AccessioniPrimary (citable) accession number: Q9SRZ6
Secondary accession number(s): Q8L9Z4, Q8RWH2, Q8RYD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2013
Last sequence update: May 1, 2000
Last modified: October 29, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3