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Protein

Cytosolic isocitrate dehydrogenase [NADP]

Gene

CICDH

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

May supply 2-oxoglutarate for amino acid biosynthesis and ammonia assimilation via the glutamine synthetase/glutamate synthase (GS/GOGAT) pathway. May be involved in the production of NADPH to promote redox signaling or homeostasis in response to oxidative stress.1 Publication

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg2+ or Mn2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei79SubstrateBy similarity1
Binding sitei84NADPBy similarity1
Binding sitei111SubstrateBy similarity1
Binding sitei134SubstrateBy similarity1
Sitei141Critical for catalysisBy similarity1
Sitei212Critical for catalysisBy similarity1
Metal bindingi252Magnesium or manganeseBy similarity1
Binding sitei260NADPBy similarity1
Metal bindingi275Magnesium or manganeseBy similarity1
Binding sitei328NADP; via amide nitrogen and carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi77 – 79NADPBy similarity3
Nucleotide bindingi310 – 315NADPBy similarity6

GO - Molecular functioni

  • copper ion binding Source: TAIR
  • isocitrate dehydrogenase (NADP+) activity Source: TAIR
  • magnesium ion binding Source: InterPro
  • NAD binding Source: InterPro

GO - Biological processi

  • defense response to bacterium Source: TAIR
  • isocitrate metabolic process Source: TAIR
  • NADP metabolic process Source: TAIR
  • response to cadmium ion Source: TAIR
  • response to salt stress Source: TAIR
  • response to zinc ion Source: TAIR
  • tricarboxylic acid cycle Source: UniProtKB-KW

Keywordsi

Molecular functionOxidoreductase
Biological processPlant defense, Stress response, Tricarboxylic acid cycle
LigandMagnesium, Manganese, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:AT1G65930-MONOMER
BRENDAi1.1.1.42 399
ReactomeiR-ATH-389542 NADPH regeneration
R-ATH-6798695 Neutrophil degranulation
R-ATH-71403 Citric acid cycle (TCA cycle)

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic isocitrate dehydrogenase [NADP] (EC:1.1.1.42)
Gene namesi
Name:CICDH
Ordered Locus Names:At1g65930
ORF Names:F12P19.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G65930
TAIRilocus:2009759 AT1G65930

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Slight reduction in plant growth. Constitutive expression of pathogenesis-related genes and enhanced resistance to the bacterial pathogen P.syringae pv. tomato.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004219631 – 410Cytosolic isocitrate dehydrogenase [NADP]Add BLAST410

Proteomic databases

PaxDbiQ9SRZ6
PRIDEiQ9SRZ6

PTM databases

iPTMnetiQ9SRZ6

Expressioni

Gene expression databases

ExpressionAtlasiQ9SRZ6 baseline and differential
GenevisibleiQ9SRZ6 AT

Interactioni

Protein-protein interaction databases

BioGridi281262 interactors.
IntActiQ9SRZ6 1 interactor.
STRINGi3702.AT1G65930.1

Structurei

3D structure databases

ProteinModelPortaliQ9SRZ6
SMRiQ9SRZ6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni96 – 102Substrate bindingBy similarity7

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1526 Eukaryota
COG0538 LUCA
HOGENOMiHOG000019858
InParanoidiQ9SRZ6
KOiK00031
OMAiIQKRWPL
OrthoDBiEOG09360AQU
PhylomeDBiQ9SRZ6

Family and domain databases

InterProiView protein in InterPro
IPR019818 IsoCit/isopropylmalate_DH_CS
IPR004790 Isocitrate_DH_NADP
IPR024084 IsoPropMal-DH-like_dom
PANTHERiPTHR11822 PTHR11822, 1 hit
PfamiView protein in Pfam
PF00180 Iso_dh, 1 hit
PIRSFiPIRSF000108 IDH_NADP, 1 hit
SMARTiView protein in SMART
SM01329 Iso_dh, 1 hit
TIGRFAMsiTIGR00127 nadp_idh_euk, 1 hit
PROSITEiView protein in PROSITE
PS00470 IDH_IMDH, 1 hit

Sequencei

Sequence statusi: Complete.

Q9SRZ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFEKIKVAN PIVEMDGDEM TRVIWKSIKD KLITPFVELD IKYFDLGLPH
60 70 80 90 100
RDATDDKVTI ESAEATKKYN VAIKCATITP DEGRVTEFGL KQMWRSPNGT
110 120 130 140 150
IRNILNGTVF REPIICKNVP KLVPGWTKPI CIGRHAFGDQ YRATDAVIKG
160 170 180 190 200
PGKLTMTFEG KDGKTETEVF TFTGEGGVAM AMYNTDESIR AFADASMNTA
210 220 230 240 250
YEKKWPLYLS TKNTILKKYD GRFKDIFQEV YEASWKSKYD AAGIWYEHRL
260 270 280 290 300
IDDMVAYALK SEGGYVWACK NYDGDVQSDF LAQGFGSLGL MTSVLVCPDG
310 320 330 340 350
KTIEAEAAHG TVTRHFRVHQ KGGETSTNSI ASIFAWTRGL AHRAKLDDNA
360 370 380 390 400
KLLDFTEKLE AACVGTVESG KMTKDLALII HGSKLSRDTY LNTEEFIDAV
410
AAELKERLNA
Length:410
Mass (Da):45,746
Last modified:May 1, 2000 - v1
Checksum:i6CC3CA11AB631D28
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti181A → V in CAD24782 (Ref. 1) Curated1
Sequence conflicti262E → K in AAM65674 (Ref. 5) Curated1
Sequence conflicti280F → L in CAD24782 (Ref. 1) Curated1
Sequence conflicti302T → S in CAD24782 (Ref. 1) Curated1
Sequence conflicti322G → A in AAM65674 (Ref. 5) Curated1
Sequence conflicti395E → D in AAM13090 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ437268 mRNA Translation: CAD24782.1
AC009513 Genomic DNA Translation: AAF06054.1
CP002684 Genomic DNA Translation: AEE34442.1
AF419575 mRNA Translation: AAL31907.1
AY045631 mRNA Translation: AAK73989.1
AY093091 mRNA Translation: AAM13090.1
AY097340 mRNA Translation: AAM19856.1
BT002400 mRNA Translation: AAO00760.1
AY088129 mRNA Translation: AAM65674.1
PIRiF96683
RefSeqiNP_176768.1, NM_105265.5
UniGeneiAt.24713

Genome annotation databases

EnsemblPlantsiAT1G65930.1; AT1G65930.1; AT1G65930
GeneIDi842905
GrameneiAT1G65930.1; AT1G65930.1; AT1G65930
KEGGiath:AT1G65930

Similar proteinsi

Entry informationi

Entry nameiICDHC_ARATH
AccessioniPrimary (citable) accession number: Q9SRZ6
Secondary accession number(s): Q8L9Z4, Q8RWH2, Q8RYD5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2013
Last sequence update: May 1, 2000
Last modified: April 25, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome