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Q9SRZ6

- ICDHC_ARATH

UniProt

Q9SRZ6 - ICDHC_ARATH

Protein

Cytosolic isocitrate dehydrogenase [NADP]

Gene

CICDH

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    May supply 2-oxoglutarate for amino acid biosynthesis and ammonia assimilation via the glutamine synthetase/glutamate synthase (GS/GOGAT) pathway. May be involved in the production of NADPH to promote redox signaling or homeostasis in response to oxidative stress.1 Publication

    Catalytic activityi

    Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei79 – 791SubstrateBy similarity
    Binding sitei84 – 841NADPBy similarity
    Binding sitei111 – 1111SubstrateBy similarity
    Binding sitei134 – 1341SubstrateBy similarity
    Sitei141 – 1411Critical for catalysisBy similarity
    Sitei212 – 2121Critical for catalysisBy similarity
    Metal bindingi252 – 2521Magnesium or manganeseBy similarity
    Binding sitei260 – 2601NADPBy similarity
    Metal bindingi275 – 2751Magnesium or manganeseBy similarity
    Binding sitei328 – 3281NADP; via amide nitrogen and carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi77 – 793NADPBy similarity
    Nucleotide bindingi310 – 3156NADPBy similarity

    GO - Molecular functioni

    1. copper ion binding Source: TAIR
    2. isocitrate dehydrogenase (NADP+) activity Source: TAIR
    3. magnesium ion binding Source: InterPro
    4. NAD binding Source: InterPro

    GO - Biological processi

    1. defense response to bacterium Source: TAIR
    2. isocitrate metabolic process Source: TAIR
    3. NADP metabolic process Source: TAIR
    4. response to cadmium ion Source: TAIR
    5. response to salt stress Source: TAIR
    6. response to zinc ion Source: TAIR
    7. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Plant defense, Stress response, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:AT1G65930-MONOMER.
    ReactomeiREACT_214122. Abnormal conversion of 2-oxoglutarate to 2-hydroxyglutarate.
    REACT_218202. NADPH regeneration.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytosolic isocitrate dehydrogenase [NADP] (EC:1.1.1.42)
    Gene namesi
    Name:CICDH
    Ordered Locus Names:At1g65930
    ORF Names:F12P19.10
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G65930.

    Subcellular locationi

    GO - Cellular componenti

    1. apoplast Source: TAIR
    2. chloroplast stroma Source: TAIR
    3. cytosol Source: TAIR
    4. plasma membrane Source: TAIR
    5. plasmodesma Source: TAIR

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Slight reduction in plant growth. Constitutive expression of pathogenesis-related genes and enhanced resistance to the bacterial pathogen P.syringae pv. tomato.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 410410Cytosolic isocitrate dehydrogenase [NADP]PRO_0000421963Add
    BLAST

    Proteomic databases

    PRIDEiQ9SRZ6.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9SRZ6.
    GenevestigatoriQ9SRZ6.

    Interactioni

    Protein-protein interaction databases

    IntActiQ9SRZ6. 1 interaction.
    STRINGi3702.AT1G65930.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SRZ6.
    SMRiQ9SRZ6. Positions 5-404.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni96 – 1027Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    InParanoidiQ9SRZ6.
    KOiK00031.
    OMAiNEHYVIT.
    PhylomeDBiQ9SRZ6.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view]
    PANTHERiPTHR11822. PTHR11822. 1 hit.
    PfamiPF00180. Iso_dh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
    PROSITEiPS00470. IDH_IMDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9SRZ6-1 [UniParc]FASTAAdd to Basket

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    MAFEKIKVAN PIVEMDGDEM TRVIWKSIKD KLITPFVELD IKYFDLGLPH    50
    RDATDDKVTI ESAEATKKYN VAIKCATITP DEGRVTEFGL KQMWRSPNGT 100
    IRNILNGTVF REPIICKNVP KLVPGWTKPI CIGRHAFGDQ YRATDAVIKG 150
    PGKLTMTFEG KDGKTETEVF TFTGEGGVAM AMYNTDESIR AFADASMNTA 200
    YEKKWPLYLS TKNTILKKYD GRFKDIFQEV YEASWKSKYD AAGIWYEHRL 250
    IDDMVAYALK SEGGYVWACK NYDGDVQSDF LAQGFGSLGL MTSVLVCPDG 300
    KTIEAEAAHG TVTRHFRVHQ KGGETSTNSI ASIFAWTRGL AHRAKLDDNA 350
    KLLDFTEKLE AACVGTVESG KMTKDLALII HGSKLSRDTY LNTEEFIDAV 400
    AAELKERLNA 410
    Length:410
    Mass (Da):45,746
    Last modified:May 1, 2000 - v1
    Checksum:i6CC3CA11AB631D28
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti181 – 1811A → V in CAD24782. 1 PublicationCurated
    Sequence conflicti262 – 2621E → K in AAM65674. 1 PublicationCurated
    Sequence conflicti280 – 2801F → L in CAD24782. 1 PublicationCurated
    Sequence conflicti302 – 3021T → S in CAD24782. 1 PublicationCurated
    Sequence conflicti322 – 3221G → A in AAM65674. 1 PublicationCurated
    Sequence conflicti395 – 3951E → D in AAM13090. (PubMed:14593172)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ437268 mRNA. Translation: CAD24782.1.
    AC009513 Genomic DNA. Translation: AAF06054.1.
    CP002684 Genomic DNA. Translation: AEE34442.1.
    AF419575 mRNA. Translation: AAL31907.1.
    AY045631 mRNA. Translation: AAK73989.1.
    AY093091 mRNA. Translation: AAM13090.1.
    AY097340 mRNA. Translation: AAM19856.1.
    BT002400 mRNA. Translation: AAO00760.1.
    AY088129 mRNA. Translation: AAM65674.1.
    PIRiF96683.
    RefSeqiNP_176768.1. NM_105265.4.
    UniGeneiAt.24713.

    Genome annotation databases

    EnsemblPlantsiAT1G65930.1; AT1G65930.1; AT1G65930.
    GeneIDi842905.
    KEGGiath:AT1G65930.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ437268 mRNA. Translation: CAD24782.1 .
    AC009513 Genomic DNA. Translation: AAF06054.1 .
    CP002684 Genomic DNA. Translation: AEE34442.1 .
    AF419575 mRNA. Translation: AAL31907.1 .
    AY045631 mRNA. Translation: AAK73989.1 .
    AY093091 mRNA. Translation: AAM13090.1 .
    AY097340 mRNA. Translation: AAM19856.1 .
    BT002400 mRNA. Translation: AAO00760.1 .
    AY088129 mRNA. Translation: AAM65674.1 .
    PIRi F96683.
    RefSeqi NP_176768.1. NM_105265.4.
    UniGenei At.24713.

    3D structure databases

    ProteinModelPortali Q9SRZ6.
    SMRi Q9SRZ6. Positions 5-404.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9SRZ6. 1 interaction.
    STRINGi 3702.AT1G65930.1-P.

    Proteomic databases

    PRIDEi Q9SRZ6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G65930.1 ; AT1G65930.1 ; AT1G65930 .
    GeneIDi 842905.
    KEGGi ath:AT1G65930.

    Organism-specific databases

    TAIRi AT1G65930.

    Phylogenomic databases

    InParanoidi Q9SRZ6.
    KOi K00031.
    OMAi NEHYVIT.
    PhylomeDBi Q9SRZ6.

    Enzyme and pathway databases

    BioCyci MetaCyc:AT1G65930-MONOMER.
    Reactomei REACT_214122. Abnormal conversion of 2-oxoglutarate to 2-hydroxyglutarate.
    REACT_218202. NADPH regeneration.

    Gene expression databases

    ArrayExpressi Q9SRZ6.
    Genevestigatori Q9SRZ6.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view ]
    PANTHERi PTHR11822. PTHR11822. 1 hit.
    Pfami PF00180. Iso_dh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsi TIGR00127. nadp_idh_euk. 1 hit.
    PROSITEi PS00470. IDH_IMDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of a full lenght cDNA encoding for NADP+-isocitrate dehydrogenase from Arabidopsis thaliana."
      Pistelli L., De Bellis L., Alpi A., Gonzali S.
      Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. "Cytosolic NADP-dependent isocitrate dehydrogenase contributes to redox homeostasis and the regulation of pathogen responses in Arabidopsis leaves."
      Mhamdi A., Mauve C., Gouia H., Saindrenan P., Hodges M., Noctor G.
      Plant Cell Environ. 33:1112-1123(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiICDHC_ARATH
    AccessioniPrimary (citable) accession number: Q9SRZ6
    Secondary accession number(s): Q8L9Z4, Q8RWH2, Q8RYD5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2013
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3