Q9SRX3 (GUN1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase 1
EC=3.2.1.4

Alternative name(s):
Cellulase 2
Short name=AtCEL2
Endo-1,4-beta glucanase 1

Gene names

Name:	CEL2
Ordered Locus Names:	At1g02800
ORF Names:	F22D16.21

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Secreted Potential.
Developmental stage	Expressed in the developing septum and ovule primordia during the early stages of flower development. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cell wall biogenesis/degradation Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW pattern specification process Traceable author statement Ref.1. Source: TAIR response to nematode Inferred from expression pattern PubMed 18069944. Source: TAIR
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cellulase activity Traceable author statement Ref.1. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Potential
	Chain	30 – 501	472	Endoglucanase 1		PRO_0000249254
Sites
	Active site	422	1	By similarity
	Active site	473	1	By similarity
	Active site	482	1	By similarity
Experimental info
	Sequence conflict	330	1	L → I in AAC16418. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9SRX3 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 913F592A058505B7	FASTA	501	55,419
	10 20 30 40 50 60 MALYLSSSRL ITFLSFILLL SNGFSSSSSR PSIHHRHHLD NHNYKDALSK SILFFEGQRS 70 80 90 100 110 120 GKLPPNQRMT WRSNSGLSDG SALNVDLVGG YYDAGDNMKF GFPMAFTTTM LSWSLIEFGG 130 140 150 160 170 180 LMKSELPNAK DAIRWATDFL LKATSHPDTI YVQVGDPNMD HACWERPEDM DTPRSVFKVD 190 200 210 220 230 240 KNNPGSDIAG EIAAALAAAS IVFRKCDPSY SNHLLQRAIT VFTFADKYRG PYSAGLAPEV 250 260 270 280 290 300 CPFYCSYSGY QDELLWGAAW LQKATNNPTY LNYIKANGQI LGADEFDNMF SWDNKHVGAR 310 320 330 340 350 360 ILLSKEFLIQ KVKSLEEYKE HADSFICSVL PGASSSQYTP GGLLFKMGES NMQYVTSTSF 370 380 390 400 410 420 LLLTYAKYLT SARTVAYCGG SVVTPARLRS IAKKQVDYLL GGNPLKMSYM VGYGLKYPRR 430 440 450 460 470 480 IHHRGSSLPS VAVHPTRIQC HDGFSLFTSQ SPNPNDLVGA VVGGPDQNDQ FPDERSDYGR 490 500 SEPATYINAP LVGALAYLAR S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of genes expressed during early Arabidopsis carpel development by mRNA differential display: characterisation of ATCEL2, a novel endo-1,4-beta-D-glucanase gene." Yung M.-H., Schaffer R., Putterill J. Plant J. 17:203-208(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE. Strain: cv. Landsberg erecta.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family." Libertini E., Li Y., McQueen-Mason S.J. J. Mol. Evol. 58:506-515(2004) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF034573 mRNA. Translation: AAC16418.1. AC009525 Genomic DNA. Translation: AAF02887.1. CP002684 Genomic DNA. Translation: AEE27471.1. AK228979 mRNA. Translation: BAF00867.1.
IPI	IPI00532724.
PIR	A86158. T52135.
RefSeq	NP_171779.1. NM_100159.2.
UniGene	At.10575.
3D structure databases
HSSP	HSSP built from PDB template 1TF4 based on UniProtKB P26221.
ProteinModelPortal	Q9SRX3.
SMR	Q9SRX3. Positions 43-498.
ModBase	Search...
Protein family/group databases
CAZy	GH9. Glycoside Hydrolase Family 9.
Proteomic databases
PaxDb	Q9SRX3.
PRIDE	Q9SRX3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G02800.1; AT1G02800.1; AT1G02800.
GeneID	839385.
KEGG	ath:AT1G02800.
Organism-specific databases
TAIR	At1g02800.
Phylogenomic databases
eggNOG	NOG05134.
HOGENOM	HOG000021033.
InParanoid	Q9SRX3.
OMA	ERNDHEH.
PhylomeDB	Q9SRX3.
ProtClustDB	PLN02266.
Gene expression databases
Genevestigator	Q9SRX3.
GermOnline	AT1G02800. Arabidopsis thaliana.
Family and domain databases
Gene3D	1.50.10.10. 1 hit.
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view]
Pfam	PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit. PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN1_ARATH

Accession

Primary (citable) accession number: Q9SRX3
Secondary accession number(s): O64949

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program