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Protein

5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 2

Gene

MS2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.1 Publication

Catalytic activityi

5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Kineticsi

  1. KM=60 µM for 5-methyltetrahydrofolate1 Publication
  1. Vmax=20.7 nmol/min/mg enzyme toward 5-methyltetrahydrofolate1 Publication

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes L-methionine from L-homocysteine (MetE route).
Proteins known to be involved in this subpathway in this organism are:
  1. 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 3, chloroplastic (MS3), 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 2 (MS2), 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1 (MS1)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from L-homocysteine (MetE route), the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei490L-homocysteineBy similarity1
Binding sitei5675-methyltetrahydrofolateBy similarity1
Binding sitei605L-homocysteineBy similarity1
Metal bindingi647Zinc 1By similarity1
Metal bindingi649Zinc 1By similarity1
Metal bindingi658Zinc 2By similarity1
Metal bindingi662Zinc 2By similarity1
Metal bindingi733Zinc 1By similarity1

GO - Molecular functioni

GO - Biological processi

  • homocysteine metabolic process Source: GO_Central
  • response to cadmium ion Source: TAIR
  • response to salt stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT3G03780-MONOMER.
MetaCyc:AT3G03780-MONOMER.
SABIO-RKQ9SRV5.
UniPathwayiUPA00051; UER00082.

Names & Taxonomyi

Protein namesi
Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 2 (EC:2.1.1.14)
Alternative name(s):
Cobalamin-independent methionine synthase 2
Short name:
AtMS2
Gene namesi
Name:MS2
Ordered Locus Names:At3g03780
ORF Names:F20H23.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G03780.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: TAIR
  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • cytosol Source: TAIR
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004243561 – 7655-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 2Add BLAST765

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki406Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ9SRV5.
PRIDEiQ9SRV5.

PTM databases

iPTMnetiQ9SRV5.

Expressioni

Tissue specificityi

Expressed in leaves, stems and siliques.1 Publication

Gene expression databases

GenevisibleiQ9SRV5. AT.

Interactioni

Protein-protein interaction databases

BioGridi6480. 2 interactors.
IntActiQ9SRV5. 1 interactor.
STRINGi3702.AT3G03780.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SRV5.
SMRiQ9SRV5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni437 – 439L-homocysteine bindingBy similarity3
Regioni521 – 5225-methyltetrahydrofolate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2263. Eukaryota.
COG0620. LUCA.
HOGENOMiHOG000246221.
InParanoidiQ9SRV5.
KOiK00549.
OMAiFVRAPEQ.
OrthoDBiEOG093602ZG.
PhylomeDBiQ9SRV5.

Family and domain databases

HAMAPiMF_00172. Meth_synth. 1 hit.
InterProiIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Met_Synth_C/arc.
[Graphical view]
PfamiPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsiTIGR01371. met_syn_B12ind. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9SRV5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASHIVGYPR MGPKRELKFA LESFWDGKSS ADDLQKVSAD LRSDIWKQMS
60 70 80 90 100
AAGIKYIPSN TFSHYDQVLD TTAMLGAVPS RYGFTSGEIG LDVYFSMARG
110 120 130 140 150
NASVPAMEMT KWFDTNYHYI VPELGPEVKF SYASHKAVNE YKEAKALGVE
160 170 180 190 200
TVPVLVGPVS YLLLSKLAKG VDKSFDLLSL LPKILPVYKE VIAELKAAGA
210 220 230 240 250
SWIQLDEPLF VMDLEGHKLQ AFSGAYAELE STLSGLNVLV ETYFADIPAE
260 270 280 290 300
AYKTLTSLKG VTAFGFDLVR GTKTIDLIKS GFPQGKYLFA GVVDGRNIWA
310 320 330 340 350
NDLAASLITL QSLEGVVGKD KLVVSTSCSL LHTAVDLINE TKLDAEIKSW
360 370 380 390 400
LAFAAQKVVE VDALAKALAG QTNESFFTAN ADALSSRRSS PRVTNESVQK
410 420 430 440 450
AAAALKGSDH RRTTEVSARL DAQQKKLNLP ILPTTTIGSF PQTVELRRVR
460 470 480 490 500
REYKAKKISE EDYVKAIKEE IKKVVDIQED LDIDVLVHGE PERNDMVEYF
510 520 530 540 550
GEQLSGFAFT ANGWVQSYGS RCVKPPVIYG DVSRPKPMTV FWSSTAQSMT
560 570 580 590 600
KRPMKGMLTG PVTILNWSFV RNDQPRHETC YQIALAIKDE VEDLEKGGIG
610 620 630 640 650
VIQIDEAALR EGLPLRKAEH SFYLDWAVHS FRITNCGVQD STQIHTHMCY
660 670 680 690 700
SNFNDIIHSI IDMDADVITI ENSRSDEKLL SVFREGVKYG AGIGPGVYDI
710 720 730 740 750
HSPRIPSTDE IADRINKMLA VLEQNILWVN PDCGLKTRKY TEVKPALKAM
760
VDAAKLIRSQ LGSAK
Length:765
Mass (Da):84,584
Last modified:May 1, 2000 - v1
Checksum:iEB478D815910E701
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti763S → N in AAK64167 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ608674 mRNA. Translation: CAE55864.1.
AC009540 Genomic DNA. Translation: AAF00639.1.
CP002686 Genomic DNA. Translation: AEE73989.1.
CP002686 Genomic DNA. Translation: AEE73990.1.
CP002686 Genomic DNA. Translation: AEE73991.1.
AY040010 mRNA. Translation: AAK64167.1.
AY150385 mRNA. Translation: AAN12930.1.
AY084559 mRNA. Translation: AAM61126.1.
RefSeqiNP_001118564.1. NM_001125092.1.
NP_187028.1. NM_111249.5.
NP_850507.1. NM_180176.4.
UniGeneiAt.18359.

Genome annotation databases

EnsemblPlantsiAT3G03780.1; AT3G03780.1; AT3G03780.
AT3G03780.2; AT3G03780.2; AT3G03780.
AT3G03780.3; AT3G03780.3; AT3G03780.
GeneIDi821147.
GrameneiAT3G03780.1; AT3G03780.1; AT3G03780.
AT3G03780.2; AT3G03780.2; AT3G03780.
AT3G03780.3; AT3G03780.3; AT3G03780.
KEGGiath:AT3G03780.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ608674 mRNA. Translation: CAE55864.1.
AC009540 Genomic DNA. Translation: AAF00639.1.
CP002686 Genomic DNA. Translation: AEE73989.1.
CP002686 Genomic DNA. Translation: AEE73990.1.
CP002686 Genomic DNA. Translation: AEE73991.1.
AY040010 mRNA. Translation: AAK64167.1.
AY150385 mRNA. Translation: AAN12930.1.
AY084559 mRNA. Translation: AAM61126.1.
RefSeqiNP_001118564.1. NM_001125092.1.
NP_187028.1. NM_111249.5.
NP_850507.1. NM_180176.4.
UniGeneiAt.18359.

3D structure databases

ProteinModelPortaliQ9SRV5.
SMRiQ9SRV5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi6480. 2 interactors.
IntActiQ9SRV5. 1 interactor.
STRINGi3702.AT3G03780.1.

PTM databases

iPTMnetiQ9SRV5.

Proteomic databases

PaxDbiQ9SRV5.
PRIDEiQ9SRV5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G03780.1; AT3G03780.1; AT3G03780.
AT3G03780.2; AT3G03780.2; AT3G03780.
AT3G03780.3; AT3G03780.3; AT3G03780.
GeneIDi821147.
GrameneiAT3G03780.1; AT3G03780.1; AT3G03780.
AT3G03780.2; AT3G03780.2; AT3G03780.
AT3G03780.3; AT3G03780.3; AT3G03780.
KEGGiath:AT3G03780.

Organism-specific databases

TAIRiAT3G03780.

Phylogenomic databases

eggNOGiKOG2263. Eukaryota.
COG0620. LUCA.
HOGENOMiHOG000246221.
InParanoidiQ9SRV5.
KOiK00549.
OMAiFVRAPEQ.
OrthoDBiEOG093602ZG.
PhylomeDBiQ9SRV5.

Enzyme and pathway databases

UniPathwayiUPA00051; UER00082.
BioCyciARA:AT3G03780-MONOMER.
MetaCyc:AT3G03780-MONOMER.
SABIO-RKQ9SRV5.

Miscellaneous databases

PROiQ9SRV5.

Gene expression databases

GenevisibleiQ9SRV5. AT.

Family and domain databases

HAMAPiMF_00172. Meth_synth. 1 hit.
InterProiIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Met_Synth_C/arc.
[Graphical view]
PfamiPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsiTIGR01371. met_syn_B12ind. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMETE2_ARATH
AccessioniPrimary (citable) accession number: Q9SRV5
Secondary accession number(s): Q94BN4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2013
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.