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Protein

5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 2

Gene

MS2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.1 Publication

Catalytic activityi

5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Kineticsi

  1. KM=60 µM for 5-methyltetrahydrofolate1 Publication
  1. Vmax=20.7 nmol/min/mg enzyme toward 5-methyltetrahydrofolate1 Publication

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes L-methionine from L-homocysteine (MetE route).
Proteins known to be involved in this subpathway in this organism are:
  1. 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 3, chloroplastic (MS3), 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 2 (MS2), 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1 (MS1)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from L-homocysteine (MetE route), the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei490L-homocysteineBy similarity1
Binding sitei5675-methyltetrahydrofolateBy similarity1
Binding sitei605L-homocysteineBy similarity1
Metal bindingi647Zinc 1By similarity1
Metal bindingi649Zinc 1By similarity1
Metal bindingi658Zinc 2By similarity1
Metal bindingi662Zinc 2By similarity1
Metal bindingi733Zinc 1By similarity1

GO - Molecular functioni

GO - Biological processi

  • homocysteine metabolic process Source: GO_Central
  • response to cadmium ion Source: TAIR
  • response to salt stress Source: TAIR

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processAmino-acid biosynthesis, Methionine biosynthesis
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT3G03780-MONOMER.
MetaCyc:AT3G03780-MONOMER.
SABIO-RKiQ9SRV5.
UniPathwayiUPA00051; UER00082.

Names & Taxonomyi

Protein namesi
Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 2 (EC:2.1.1.14)
Alternative name(s):
Cobalamin-independent methionine synthase 2
Short name:
AtMS2
Gene namesi
Name:MS2
Ordered Locus Names:At3g03780
ORF Names:F20H23.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G03780.
TAIRilocus:2079434. AT3G03780.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004243561 – 7655-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 2Add BLAST765

Proteomic databases

PaxDbiQ9SRV5.
PRIDEiQ9SRV5.

PTM databases

iPTMnetiQ9SRV5.

Expressioni

Tissue specificityi

Expressed in leaves, stems and siliques.1 Publication

Gene expression databases

ExpressionAtlasiQ9SRV5. baseline and differential.
GenevisibleiQ9SRV5. AT.

Interactioni

Protein-protein interaction databases

BioGridi6480. 2 interactors.
IntActiQ9SRV5. 1 interactor.
STRINGi3702.AT3G03780.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SRV5.
SMRiQ9SRV5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni437 – 439L-homocysteine bindingBy similarity3
Regioni521 – 5225-methyltetrahydrofolate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2263. Eukaryota.
COG0620. LUCA.
HOGENOMiHOG000246221.
InParanoidiQ9SRV5.
KOiK00549.
OMAiLESHILW.
OrthoDBiEOG093602ZG.
PhylomeDBiQ9SRV5.

Family and domain databases

HAMAPiMF_00172. Meth_synth. 1 hit.
InterProiView protein in InterPro
IPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Met_Synth_C/arc.
PfamiView protein in Pfam
PF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
PIRSFiPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsiTIGR01371. met_syn_B12ind. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9SRV5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASHIVGYPR MGPKRELKFA LESFWDGKSS ADDLQKVSAD LRSDIWKQMS
60 70 80 90 100
AAGIKYIPSN TFSHYDQVLD TTAMLGAVPS RYGFTSGEIG LDVYFSMARG
110 120 130 140 150
NASVPAMEMT KWFDTNYHYI VPELGPEVKF SYASHKAVNE YKEAKALGVE
160 170 180 190 200
TVPVLVGPVS YLLLSKLAKG VDKSFDLLSL LPKILPVYKE VIAELKAAGA
210 220 230 240 250
SWIQLDEPLF VMDLEGHKLQ AFSGAYAELE STLSGLNVLV ETYFADIPAE
260 270 280 290 300
AYKTLTSLKG VTAFGFDLVR GTKTIDLIKS GFPQGKYLFA GVVDGRNIWA
310 320 330 340 350
NDLAASLITL QSLEGVVGKD KLVVSTSCSL LHTAVDLINE TKLDAEIKSW
360 370 380 390 400
LAFAAQKVVE VDALAKALAG QTNESFFTAN ADALSSRRSS PRVTNESVQK
410 420 430 440 450
AAAALKGSDH RRTTEVSARL DAQQKKLNLP ILPTTTIGSF PQTVELRRVR
460 470 480 490 500
REYKAKKISE EDYVKAIKEE IKKVVDIQED LDIDVLVHGE PERNDMVEYF
510 520 530 540 550
GEQLSGFAFT ANGWVQSYGS RCVKPPVIYG DVSRPKPMTV FWSSTAQSMT
560 570 580 590 600
KRPMKGMLTG PVTILNWSFV RNDQPRHETC YQIALAIKDE VEDLEKGGIG
610 620 630 640 650
VIQIDEAALR EGLPLRKAEH SFYLDWAVHS FRITNCGVQD STQIHTHMCY
660 670 680 690 700
SNFNDIIHSI IDMDADVITI ENSRSDEKLL SVFREGVKYG AGIGPGVYDI
710 720 730 740 750
HSPRIPSTDE IADRINKMLA VLEQNILWVN PDCGLKTRKY TEVKPALKAM
760
VDAAKLIRSQ LGSAK
Length:765
Mass (Da):84,584
Last modified:May 1, 2000 - v1
Checksum:iEB478D815910E701
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti763S → N in AAK64167 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ608674 mRNA. Translation: CAE55864.1.
AC009540 Genomic DNA. Translation: AAF00639.1.
CP002686 Genomic DNA. Translation: AEE73989.1.
CP002686 Genomic DNA. Translation: AEE73990.1.
CP002686 Genomic DNA. Translation: AEE73991.1.
AY040010 mRNA. Translation: AAK64167.1.
AY150385 mRNA. Translation: AAN12930.1.
AY084559 mRNA. Translation: AAM61126.1.
RefSeqiNP_001118564.1. NM_001125092.1.
NP_187028.1. NM_111249.5.
NP_850507.1. NM_180176.4.
UniGeneiAt.18359.

Genome annotation databases

EnsemblPlantsiAT3G03780.1; AT3G03780.1; AT3G03780.
AT3G03780.2; AT3G03780.2; AT3G03780.
AT3G03780.3; AT3G03780.3; AT3G03780.
GeneIDi821147.
GrameneiAT3G03780.1; AT3G03780.1; AT3G03780.
AT3G03780.2; AT3G03780.2; AT3G03780.
AT3G03780.3; AT3G03780.3; AT3G03780.
KEGGiath:AT3G03780.

Similar proteinsi

Entry informationi

Entry nameiMETE2_ARATH
AccessioniPrimary (citable) accession number: Q9SRV5
Secondary accession number(s): Q94BN4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2013
Last sequence update: May 1, 2000
Last modified: August 30, 2017
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families