Q9SRQ2 (CHAT_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
(Z)-3-hexen-1-ol acetyltransferase
EC=2.3.1.195

Gene names

Name:	CHAT
Ordered Locus Names:	At3g03480
ORF Names:	T21P5.10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	454 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acyltransferase involved in the production of green leaf volatiles. Uses acetyl-CoA as substrate, but not malonyl-CoA or benzoyl-CoA. Prefers primary, medium-chain-length, aliphatic alcohols. Ref.2
Catalytic activity	Acetyl-CoA + (3Z)-hex-3-en-ol = (3Z)-hex-3-en-1-yl acetate + CoA. Ref.1 Ref.2
Enzyme regulation	Inhibited by magnesium, calcium, cobalt, zinc and copper. Ref.2
Tissue specificity	Expressed in leaves and stems. Lower levels in flowers and barely detected in roots and siliques. Ref.2
Induction	Up-regulated by wounding with a peak 3 hours after wounding. Ref.2
Disruption phenotype	Loss of (3Z)-hex-3-en-1-yl acetate production. Ref.2
Sequence similarities	Belongs to the plant acetyltransferase family.
Biophysicochemical properties	Kinetic parameters: KM=10.5 µM for acetyl CoA Ref.1 Ref.2 KM=165 µM for (3Z)-hex-3-en-ol KM=174 µM for 1-octanol pH dependence: Optimum pH is 7.1-7.3.

Ontologies

Keywords
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	green leaf volatile biosynthetic process Traceable author statement Ref.2. Source: TAIR
Molecular_function	acetyl CoA:(Z)-3-hexen-1-ol acetyltransferase activity Inferred from direct assay Ref.2. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 454	454	(Z)-3-hexen-1-ol acetyltransferase		PRO_0000409589
Experimental info
	Sequence conflict	338	1	A → V in AAM61186. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q9SRQ2 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: C684495199FCCFB2	FASTA	454	50,290
	10 20 30 40 50 60 MDHQVSLPQS TTTGLSFKVH RQQRELVTPA KPTPRELKPL SDIDDQQGLR FQIPVIFFYR 70 80 90 100 110 120 PNLSSDLDPV QVIKKALADA LVYYYPFAGR LRELSNRKLA VDCTGEGVLF IEAEADVALA 130 140 150 160 170 180 ELEEADALLP PFPFLEELLF DVEGSSDVLN TPLLLVQVTR LKCCGFIFAL RFNHTMTDGA 190 200 210 220 230 240 GLSLFLKSLC ELACGLHAPS VPPVWNRHLL TVSASEARVT HTHREYDDQV GIDVVATGHP 250 260 270 280 290 300 LVSRSFFFRA EEISAIRKLL PPDLHNTSFE ALSSFLWRCR TIALNPDPNT EMRLTCIINS 310 320 330 340 350 360 RSKLRNPPLE PGYYGNVFVI PAAIATARDL IEKPLEFALR LIQETKSSVT EDYVRSVTAL 370 380 390 400 410 420 MATRGRPMFV ASGNYIISDL RHFDLGKIDF GPWGKPVYGG TAKAGIALFP GVSFYVPFKN 430 440 450 KKGETGTVVA ISLPVRAMET FVAELNGVLN VSKG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of an acyltransferase capable of synthesizing benzylbenzoate and other volatile esters in flowers and damaged leaves of Clarkia breweri." D'Auria J.C., Chen F., Pichersky E. Plant Physiol. 130:466-476(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
[2]	"Characterization of a BAHD acyltransferase responsible for producing the green leaf volatile (Z)-3-hexen-1-yl acetate in Arabidopsis thaliana." D'Auria J.C., Pichersky E., Schaub A., Hansel A., Gershenzon J. Plant J. 49:194-207(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, TISSUE SPECIFICITY, INDUCTION BY WOUNDING, FUNCTION. Strain: cv. Landsberg erecta.
[3]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF500201 mRNA. Translation: AAN09797.1. AC009895 Genomic DNA. Translation: AAF01587.1. CP002686 Genomic DNA. Translation: AEE73949.1. AY084623 mRNA. Translation: AAM61186.1.
IPI	IPI00537056.
RefSeq	NP_186998.1. NM_111219.2.
UniGene	At.40974.
3D structure databases
ProteinModelPortal	Q9SRQ2.
SMR	Q9SRQ2. Positions 18-441.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT3G03480.1-P.
Proteomic databases
PRIDE	Q9SRQ2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	821249.
KEGG	ath:AT3G03480.
Organism-specific databases
TAIR	At3g03480.
Phylogenomic databases
eggNOG	NOG323053.
HOGENOM	HOG000237410.
InParanoid	Q9SRQ2.
OMA	IPVIQFY.
PhylomeDB	Q9SRQ2.
ProtClustDB	CLSN2684090.
Enzyme and pathway databases
BioCyc	MetaCyc:AT3G03480-MONOMER.
Gene expression databases
Genevestigator	Q9SRQ2.
Family and domain databases
Gene3D	3.30.559.10. 2 hits.
InterPro	IPR023213. CAT-like_dom. IPR003480. Transferase. [Graphical view]
Pfam	PF02458. Transferase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHAT_ARATH

Accession

Primary (citable) accession number: Q9SRQ2
Secondary accession number(s): Q8LFV6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 28, 2011
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program