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Protein

Cytosolic endo-beta-N-acetylglucosaminidase 2

Gene

ENGASE2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Endoglycosidase that releases N-glycans from glycoproteins by cleaving the beta-1,4-glycosidic bond in the N,N'-diacetylchitobiose core. Involved in the production of high-mannose type N-glycans during plant development and fruit maturation.2 Publications

Catalytic activityi

Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)2)Asn-structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact.2 Publications

Kineticsi

  1. KM=0.1 mM for dabsyl-Asn-Man51 Publication

    pH dependencei

    Optimum pH is 6.0-7.0.1 Publication

    GO - Molecular functioni

    • mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity Source: TAIR

    GO - Biological processi

    • N-glycan processing Source: TAIR
    • protein deglycosylation Source: TAIR

    Keywordsi

    Molecular functionGlycosidase, Hydrolase

    Enzyme and pathway databases

    ReactomeiR-ATH-532668 N-glycan trimming in the ER and Calnexin/Calreticulin cycle

    Protein family/group databases

    CAZyiGH85 Glycoside Hydrolase Family 85

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytosolic endo-beta-N-acetylglucosaminidase 2Curated (EC:3.2.1.962 Publications)
    Short name:
    ENGase 2Curated
    Alternative name(s):
    Endo-beta-N-acetyglucosaminidase 85B1 Publication
    Short name:
    AtENGase85B1 Publication
    Gene namesi
    Name:ENGASE2Curated
    Synonyms:ENGASE85B1 Publication
    Ordered Locus Names:At3g11040Imported
    ORF Names:F9F8.14Imported
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 3

    Organism-specific databases

    AraportiAT3G11040
    TAIRilocus:2074598 AT3G11040

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype under normal growth conditions, but the double mutants engase85A and engase85B accumulate very high level of free N-glycans carrying two GlcNAc at the reducing end, but their counterparts with a single GlcNAc are completely absent.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004327571 – 701Cytosolic endo-beta-N-acetylglucosaminidase 2Add BLAST701

    Proteomic databases

    PaxDbiQ9SRL4

    Expressioni

    Gene expression databases

    ExpressionAtlasiQ9SRL4 baseline and differential
    GenevisibleiQ9SRL4 AT

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT3G11040.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SRL4
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 85 family.Curated

    Phylogenomic databases

    eggNOGiKOG2331 Eukaryota
    COG4724 LUCA
    HOGENOMiHOG000241368
    OMAiHEASLVM
    OrthoDBiEOG0936041J
    PhylomeDBiQ9SRL4

    Family and domain databases

    Gene3Di2.60.120.260, 1 hit
    InterProiView protein in InterPro
    IPR032979 ENGase
    IPR008979 Galactose-bd-like_sf
    IPR005201 Glyco_hydro_85
    IPR017853 Glycoside_hydrolase_SF
    PANTHERiPTHR13246 PTHR13246, 1 hit
    PfamiView protein in Pfam
    PF03644 Glyco_hydro_85, 1 hit
    SUPFAMiSSF51445 SSF51445, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q9SRL4-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MPKSNDDDVA QSEAVPLLDL VKPSLPISFP IKALQDLKSR SYFDSFHFQF
    60 70 80 90 100
    NRSTVPFRRN SDCLPNRPRV LVCHDMKGGY VDDKWVQGCE NEAGFAIWHW
    110 120 130 140 150
    YLMDIFVYFS HSLVTIPPPC WTNTAHRHGV KVLGTFITEW DEGKATCKEM
    160 170 180 190 200
    LATKESAQMY AERLAELATA LGFDGWLINI ENDIDEEQIP NMKEFVSHLK
    210 220 230 240 250
    KVLHLSTPGA LVIWYDSVTV RGNLQWQDQL TELNKPFFDL CDGIFMNYTW
    260 270 280 290 300
    KESYPNLSAE VAGDRKFDVY MGIDVFGRGS FGGGQWTVNA ALDLLKRNNV
    310 320 330 340 350
    SAAIFAPGWV YETAQPPNFH TAQNKWWSLV EKSWGIVQTY PQVLPFYSDF
    360 370 380 390 400
    NQGFGYHVSL EGRQLSDSPW YNISCQSLQP LLEFNEDNKD IIQVTVDQEG
    410 420 430 440 450
    KNVFDFSEQH LNNYYEYDSA REASFNGGGN IVFRGKLKGD AYFTTRLFKP
    460 470 480 490 500
    HLQLSSSPIT ISYSVKSDET SNLGILLSFS SPSLETKSIL VAPEDPIRRF
    510 520 530 540 550
    DDMSLQCLTT SVQTVSEWTV HEASLVMDGH TLTEISAFCY RPENSTKSAE
    560 570 580 590 600
    FVALLGHISV KDHVQNQQNP EILLPASSWV IEAHNVELVP GNSSSKILRV
    610 620 630 640 650
    KLEWRQKDLE DSAFTRYNVY AENVKSTDLR PRKVLEKPKS ETVLLGIAHV
    660 670 680 690 700
    PAYYVAELVV ESDVKAVRFM VQACGEDASL GKLDEALNLL VDLEGLSVNH

    D
    Length:701
    Mass (Da):79,325
    Last modified:May 1, 2000 - v1
    Checksum:iA9AFC5433BBBA12D
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC009991 Genomic DNA Translation: AAF01517.1
    CP002686 Genomic DNA No translation available.

    Genome annotation databases

    EnsemblPlantsiAT3G11040.3; AT3G11040.3; AT3G11040
    GrameneiAT3G11040.3; AT3G11040.3; AT3G11040

    Similar proteinsi

    Entry informationi

    Entry nameiENAS2_ARATH
    AccessioniPrimary (citable) accession number: Q9SRL4
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2015
    Last sequence update: May 1, 2000
    Last modified: May 23, 2018
    This is version 106 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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